1,721,673 research outputs found
Statistics of variant analysis in heterozygous sites in genome of CBS 11270 and between genomes of CBS 11270 and CBS 2499.
No full textStatistics of variant analysis in heterozygous sites in genome of CBS 11270 and between genomes of CBS 11270 and CBS 2499.</p
Counts of different types of nucleotide transversions and transitions in heterozygous sites in the genome of CBS 11270 and between the genomes of CBS 11270 and CBS 2499.
No full textCounts of different types of nucleotide transversions and transitions in heterozygous sites in the genome of CBS 11270 and between the genomes of CBS 11270 and CBS 2499.</p
MEN 11270, a novel selective constrained peptide antagonist with high affinity at the human B-2 kinin receptor
No full textWe investigated the pharmacological profile of MEN 11270, or H-D-Arg-Arg-Pro-Hyp-Gly-Thi-c(Dab-DTic-Oic-Arg)c(7gamma-10 alpha), a conformationally constrained derivative of the B2 kinin receptor antagonist Icatibant. MEN 11270 bound with high-affinity to the B2 kinin receptor constitutively expressed by WI38 human fibroblasts, inhibiting 3H-bradykinin (BK) with a pKi value of 10.3 +/- 0.08 (n = 5). The rank order of affinity of several peptide and nonpeptide antagonists was also assessed: Icatibant (pKi = 10.6) approximately MEN 11270 (pKi = 10.3) approximately B9430 (pKi = 10.0) > B9858 (pKi = 8.0) > FR173657 (pKi = 7.6) > WIN64338 (pKi = 7.2) > Lys-[des-Arg9, Leu8]-BK (pKi [des-Arg9,Leu8]-BK (pKi < 5). MEN 11270 showed a low affinity in inhibiting 3H-Lys-[des-Arg9]-BK binding at the human B1 kinin receptor constitutively expressed by the same cells (pKi 6.0 +/- 0.33; n = 3). MEN 11270 showed no binding affinity (pIC50 < 5.5) at 29 different receptors and ion channels. In the human umbilical vein contraction assay, MEN 11270, shifted the concentration-response curve to BK to the right in a concentration-dependent manner (pA2 8.14 +/- 0.22, n = 7). The Schild plot was linear (slope 0.95 +/- 0.11), consistent with a competitive antagonism. In the same bioassay, MEN 11270 (10 microM) did not affect the concentration-response curve to the B1 agonist Lys-[des-Arg9]-BK nor the contractile responses elicited by noradrenaline or serotonin. These findings indicate MEN 11270 as an antagonist at the human B2 kinin receptor, with potency and selectivity comparable to those of the linear peptide antagonist, supporting the hypothesis that a constrained C-terminal beta-turn conformation preserves a high affinity for the interaction of Icatibant with the B2 kinin receptor
Chromosomal genome assembly of the ethanol production strain CBS 11270 indicates a highly dynamic genome structure in the yeast species <i>Brettanomyces bruxellensis</i> - Fig 4
No full textDistribution of indels of different size in heterozygous sites in the genome of CBS 11270 (A) and between genomes of CBS 11270 and CBS 2499 (B).</p
Survey of the genes with the highest numbers of SNPs in CBS 11270 and CBS 2499.
No full textSurvey of the genes with the highest numbers of SNPs in CBS 11270 and CBS 2499.</p
Annotation details of the <i>B</i>. <i>bruxellensis</i> CBS 11270 nuclear genome.
No full textAnnotation details of the B. bruxellensis CBS 11270 nuclear genome.</p
Genes in the <i>B</i>. <i>bruxellensis</i> CBS 2499 genome absent in the CBS 11270 genome.
No full textGenes in the B. bruxellensis CBS 2499 genome absent in the CBS 11270 genome.</p
Chromosomal genome assembly of the ethanol production strain CBS 11270 indicates a highly dynamic genome structure in the yeast species <i>Brettanomyces bruxellensis</i> - Fig 2
No full textLocation of certain genes (A) and duplicated genes (B) on the chromosomes of CBS 11270.</p
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
- …
