1,721,843 research outputs found
Viability of mouse peritoneal cells after challenge with <i>B. cereus</i> ATCC 10876 secreted proteins.
No full text<p>(A) Flow cytometric assessment of the percentage of propidium iodide-stained cells isolated from the peritoneal cavity of mice (n = 4/group) injected with 500 µl of diluted supernatants of <i>B. cereus</i> ATCC 10876 or <i>B. cereus</i> PlcR mutant, or with heat-treated supernatants of <i>B. cereus</i> 10876. Bars labeled with “10876” represent 10 min (dark blue) and 45 min (lighter blue) incubation times after injection. (B) Representative forward vs. side scatter plots of cells retrieved from the peritoneal cavity of mice 45 min after injection with supernatants of <i>B. cereus</i> ATCC 10876 (left panel), the <i>B. cereus</i> PlcR mutant (middle panel), or heat-treated supernatants of <i>B. cereus</i> 10876 (right panel).</p
Expression profile of Hbl proteins in <i>B. cereus</i> ATCC 10876.
No full text<p>(A) Growth measurements (OD at 600 nm) of <i>B. cereus</i> ATCC 10876 grown in Brain Heart Infusion broth over time. Arrows indicate points at which samples were withdrawn for analyses performed in (B) and (C). (B) Viability assessment of macrophages incubated for 90 min with <i>B. cereus</i> ATCC 10876 sterile, serially-diluted culture supernatants. (C) Western blot analysis of growth-phase dependent expression and accumulation of Hbl toxin components B, L<sub>1</sub>, and L<sub>2</sub> of <i>B. cereus</i> ATCC 10876 in sterile culture supernatants. Toxin proteins were detected using mouse serum containing antibodies raised against single toxin components.</p
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Identification of the toxic components present in <i>B. cereus</i> ATCC 10876 culture supernatants.
No full text<p>(A) RAW264.7 macrophage toxicity measurement of protein-containing <i>B. cereus</i> ATCC 10876 culture supernatant fractions eluted from anion exchange column. FT, flow through fraction; Input, unfractionated supernatant. (B) Coomassie-stained gel of combined, toxic anion exchange fractions. Arrows indicate bands subjected to mass spectrometric analysis.</p
Cytotoxicity of proteins secreted by <i>B. cereus</i> ATCC 10876.
No full text<p>Cells of different type and origin were challenged for 90<i>B. cereus</i> ATCC 10876 (solid lines) or an isogenic mutant defective in the expression of the global regulator PlcR (dotted lines). Viability was assessed by the ability of cells to convert 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide to purple formazan and is expressed as % of the untreated control.</p
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
- …
