1,721,705 research outputs found
The (-)10431-10566 RNA fragment is a functional template for the initiation of RNA synthesis.
No full text(A) EMSA showing the interaction between the (-)10431-10566 RNA with the purified recombinant RdRp protein. Uniformly DIG-labeled (-)10431-10566 RNA (0.25 pmol) or nonspecific RNA (nsRNA) from 5’ 152-nt of bovine coronavirus genome were titrated with increasing concentrations of RdRp (or BSA) from 0.25, 0.5, 1 to 2.5 μM. The complexes were resolved on a 5% native polyacrylamide gel. Position of the free probe and RNA-protein complexes (RPC) are indicated. (B) Specific interaction of RdRp with the (-)10431-10566 RNA. All reactions contained the same amount of DIG-labeled (-)10431-10566 RNA (0.25 pmol). (Lane 1) free probe; (lanes 2–11) 2.5 μM of RdRp were used; (lane 2) no competitor; (lanes 3–11) unlabeled RNA competitors, representing either (-)10431-10566, tRNA, or nsRNA (10-, 50-, 100-fold molar excess) as indicated, were added to the gel-shift assay to compete against complex formation. The complexes were resolved on a 5% native polyacrylamide gel. (C) Template specificity of the purified RdRp. Templates tested were nsRNA and (+)1-160 RNA (the SLA-containing promoter element) as indicated on the top. (D) Transcripts driven by T7 RNA polymerase used as size markers (lanes 1–5) or RNA templates used for in vitro RdRp assays (lanes 6–10) are shown on the top. The products were resolved on 6% polyacrylamide gel containing 6M urea. (E) Schematic diagram of the minus RNA fragments used as templates for the in vitro RdRp assays. The numbers correspond to the nucleotide positions in the JEV genome. The products are depicted as de novo synthesis or transcription products with size (nts) indicated. (F) Diagram of the secondary structure of the 3’-UTR up to nt 10566 with relative position marked for the templates used by in vitro RdRp assays.</p
Human Capital and Economic Growth in Pakistan
Get PDFPakistan’s economy has grown faster on average than many other low- and middleincome countries over the past two decades. But several countries in Southeast Asia have fared even better. This paper focuses on factors that explain Pakistan’s relative growth performance. In addition to more traditional factors believed to determine growth, this paper looks particularly at the role of differences in the quality of human capital. The cross-country empirical results suggest that accumulation of physical capital and improvements in the quality of institutions have the largest pay-offs in terms of achieving higher growth, but that better education and health care also have a significant impact. Investment in these areas will increase the possibility of Pakistan entering a virtuous cycle of high growth and improved living conditions for the population.
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
A Sketch of the Life of Elizabeth T. Stone, and of Her Persecution, with an Appendix of Her Treatment and Sufferings While in the Charleston McLean Asylum Where She was Confined Under the Pretense of Insanity
No full texthttps://kent-islandora.s3.us-east-2.amazonaws.com/node/10566/10896-thumbnail.jpgPrinted for the Author, Boston: The Autho
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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