1,720,955 research outputs found
Ekstrakcija kolagena iz meduza: optimizacija metode
No full textJellyfish are a novel source for collagen extraction, and it is derived from
multiple Medusozoa species, including Rhizostoma pulmo. Their collagen is
evolutionary older and simpler than mammalian collagens making them
compatible with human biology. Its specific response to macrophages - a
lower M1 macrophage response and a higher M2 macrophage response -
triggers regeneration in tissue, making jellyfish collagen a candidate
biomaterial for tissue engineering. To compare the collagen composition,
multiple sequence alignment was performed between different types of
human collagen and between murine collagen using SALIGN. Sequence
alignment of fibrillar collagens showed similarities in characteristic glycine
and proline regions, and in lysine sites in the sequence. Amino acid analysis
showed several differences in composition between different collagen
sources. The extraction of collagen from jellyfish consists of three main
steps: sample preparation, extraction, and recovery, and can last up to 7
days. This lengthy procedure can lead to collagen degradation and a
decrease in final yield. Current methods consist of tissue cutting and
chemical pretreatment during sample preparation, acid and enzymatic
extraction, and dialysis. The goal of this research was to see how changes
in the extraction steps affect collagen yield, purity, and the methods
duration. For this purpose, samples from Rhizostoma pulmo were first
lyophilized, followed by acid extraction with 0,5 M acetic acid, salting out
with NaCl, and purification with C18 cartridges. The obtained collagen
powder was analyzed using MALDI-TOF-MS to confirm the successful
extraction of collagen. In addition, FTIR and SDS-PAGE were performed to
further confirm the presence of collagen. The collagen yields that were
obtained are similar to those recorded in previous studies. Based on these
results, it can be concluded that the use of C18 cartridges can be an
alternative for dialysis and could shorten the extraction process.Meduze su nov izvor kolagena, a kolagen se dobiva iz nekoliko vrsta
Medusozoa, uključujući Rhizostoma pulmo. Kolagen iz meduza je evolucijski
stariji i jednostavniji od kolagena sisavaca, što ga čini kompatibilnim s
ljudskom biologijom. Njegov specifičan odgovor na makrofage - niži
odgovor M1 makrofaga i viši odgovor M2 makrofaga - potiče regeneraciju
tkiva, što čini kolagen meduza potencijalnim biomaterijalom u tkivnom
inženjerstvu. Da bi se usporedio sastav kolagena, višestruko poravnanje
sekvenci (multiple sequence alignment) je izvršeno između različitih tipova
ljudskog kolagena i između mišjeg kolagena koristeći SALIGN. Poravnanje
sekvenci fibrilarnih kolagena pokazalo je sličnosti u karakterističnim
glicinskim i prolinskim regijama te u mjestima na kojima su lizini u
sekvencama. Analiza aminokiselina pokazala je nekoliko razlika u sastavu
između različitih izvora kolagena.
Ekstrakcija kolagena iz meduza sastoji se od tri glavna koraka: pripreme
uzorka, ekstrakcije i oporavka, i može trajati do 7 dana. Ovaj dugotrajan
postupak može dovesti do degradacije kolagena i smanjenja konačnog
prinosa. Trenutne metode uključuju usitnjavanje tkiva i kemijsko tretiranje
tokom pripreme uzorka, kiselinsku i enzimsku ekstrakciju, kao i dijalizu. Cilj
ovog istraživanja bio je utvrditi kako promjene u koracima ekstrakcije utiču
na prinos, čistoću kolagena i trajanje metode. U tu svrhu, uzorci vrste
Rhizostoma pulmo su prvo liofilizirani, nakon čega je uslijedila ekstrakcija
kiselinom koristeći 0,5 M octenu kiselinu, isoljavanje pomoću NaCl i
pročišćavanje uz pomoć C18 kolona. Dobiveni kolagen je analiziran pomoću
MALDI-a kako bi se potvrdila uspješna ekstrakcija kolagena. Također, FTIR
i SDS-PAGE su odrađeni radi dodatne potvrde prisustva kolagena u izolatu.
Prinosi kolagena koji su dobiveni slični su onima zabilježenim u prethodnim
studijama. Na osnovu ovih rezultata, može se zaključiti da upotreba C18
kolona može biti alternativa za dijalizu i mogla bi skratiti proces ekstrakcije
Ekstrakcija kolagena iz meduza: optimizacija metode
No full textJellyfish are a novel source for collagen extraction, and it is derived from
multiple Medusozoa species, including Rhizostoma pulmo. Their collagen is
evolutionary older and simpler than mammalian collagens making them
compatible with human biology. Its specific response to macrophages - a
lower M1 macrophage response and a higher M2 macrophage response -
triggers regeneration in tissue, making jellyfish collagen a candidate
biomaterial for tissue engineering. To compare the collagen composition,
multiple sequence alignment was performed between different types of
human collagen and between murine collagen using SALIGN. Sequence
alignment of fibrillar collagens showed similarities in characteristic glycine
and proline regions, and in lysine sites in the sequence. Amino acid analysis
showed several differences in composition between different collagen
sources. The extraction of collagen from jellyfish consists of three main
steps: sample preparation, extraction, and recovery, and can last up to 7
days. This lengthy procedure can lead to collagen degradation and a
decrease in final yield. Current methods consist of tissue cutting and
chemical pretreatment during sample preparation, acid and enzymatic
extraction, and dialysis. The goal of this research was to see how changes
in the extraction steps affect collagen yield, purity, and the methods
duration. For this purpose, samples from Rhizostoma pulmo were first
lyophilized, followed by acid extraction with 0,5 M acetic acid, salting out
with NaCl, and purification with C18 cartridges. The obtained collagen
powder was analyzed using MALDI-TOF-MS to confirm the successful
extraction of collagen. In addition, FTIR and SDS-PAGE were performed to
further confirm the presence of collagen. The collagen yields that were
obtained are similar to those recorded in previous studies. Based on these
results, it can be concluded that the use of C18 cartridges can be an
alternative for dialysis and could shorten the extraction process.Meduze su nov izvor kolagena, a kolagen se dobiva iz nekoliko vrsta
Medusozoa, uključujući Rhizostoma pulmo. Kolagen iz meduza je evolucijski
stariji i jednostavniji od kolagena sisavaca, što ga čini kompatibilnim s
ljudskom biologijom. Njegov specifičan odgovor na makrofage - niži
odgovor M1 makrofaga i viši odgovor M2 makrofaga - potiče regeneraciju
tkiva, što čini kolagen meduza potencijalnim biomaterijalom u tkivnom
inženjerstvu. Da bi se usporedio sastav kolagena, višestruko poravnanje
sekvenci (multiple sequence alignment) je izvršeno između različitih tipova
ljudskog kolagena i između mišjeg kolagena koristeći SALIGN. Poravnanje
sekvenci fibrilarnih kolagena pokazalo je sličnosti u karakterističnim
glicinskim i prolinskim regijama te u mjestima na kojima su lizini u
sekvencama. Analiza aminokiselina pokazala je nekoliko razlika u sastavu
između različitih izvora kolagena.
Ekstrakcija kolagena iz meduza sastoji se od tri glavna koraka: pripreme
uzorka, ekstrakcije i oporavka, i može trajati do 7 dana. Ovaj dugotrajan
postupak može dovesti do degradacije kolagena i smanjenja konačnog
prinosa. Trenutne metode uključuju usitnjavanje tkiva i kemijsko tretiranje
tokom pripreme uzorka, kiselinsku i enzimsku ekstrakciju, kao i dijalizu. Cilj
ovog istraživanja bio je utvrditi kako promjene u koracima ekstrakcije utiču
na prinos, čistoću kolagena i trajanje metode. U tu svrhu, uzorci vrste
Rhizostoma pulmo su prvo liofilizirani, nakon čega je uslijedila ekstrakcija
kiselinom koristeći 0,5 M octenu kiselinu, isoljavanje pomoću NaCl i
pročišćavanje uz pomoć C18 kolona. Dobiveni kolagen je analiziran pomoću
MALDI-a kako bi se potvrdila uspješna ekstrakcija kolagena. Također, FTIR
i SDS-PAGE su odrađeni radi dodatne potvrde prisustva kolagena u izolatu.
Prinosi kolagena koji su dobiveni slični su onima zabilježenim u prethodnim
studijama. Na osnovu ovih rezultata, može se zaključiti da upotreba C18
kolona može biti alternativa za dijalizu i mogla bi skratiti proces ekstrakcije
Ekstrakcija kolagena iz meduza: optimizacija metode
No full textJellyfish are a novel source for collagen extraction, and it is derived from
multiple Medusozoa species, including Rhizostoma pulmo. Their collagen is
evolutionary older and simpler than mammalian collagens making them
compatible with human biology. Its specific response to macrophages - a
lower M1 macrophage response and a higher M2 macrophage response -
triggers regeneration in tissue, making jellyfish collagen a candidate
biomaterial for tissue engineering. To compare the collagen composition,
multiple sequence alignment was performed between different types of
human collagen and between murine collagen using SALIGN. Sequence
alignment of fibrillar collagens showed similarities in characteristic glycine
and proline regions, and in lysine sites in the sequence. Amino acid analysis
showed several differences in composition between different collagen
sources. The extraction of collagen from jellyfish consists of three main
steps: sample preparation, extraction, and recovery, and can last up to 7
days. This lengthy procedure can lead to collagen degradation and a
decrease in final yield. Current methods consist of tissue cutting and
chemical pretreatment during sample preparation, acid and enzymatic
extraction, and dialysis. The goal of this research was to see how changes
in the extraction steps affect collagen yield, purity, and the methods
duration. For this purpose, samples from Rhizostoma pulmo were first
lyophilized, followed by acid extraction with 0,5 M acetic acid, salting out
with NaCl, and purification with C18 cartridges. The obtained collagen
powder was analyzed using MALDI-TOF-MS to confirm the successful
extraction of collagen. In addition, FTIR and SDS-PAGE were performed to
further confirm the presence of collagen. The collagen yields that were
obtained are similar to those recorded in previous studies. Based on these
results, it can be concluded that the use of C18 cartridges can be an
alternative for dialysis and could shorten the extraction process.Meduze su nov izvor kolagena, a kolagen se dobiva iz nekoliko vrsta
Medusozoa, uključujući Rhizostoma pulmo. Kolagen iz meduza je evolucijski
stariji i jednostavniji od kolagena sisavaca, što ga čini kompatibilnim s
ljudskom biologijom. Njegov specifičan odgovor na makrofage - niži
odgovor M1 makrofaga i viši odgovor M2 makrofaga - potiče regeneraciju
tkiva, što čini kolagen meduza potencijalnim biomaterijalom u tkivnom
inženjerstvu. Da bi se usporedio sastav kolagena, višestruko poravnanje
sekvenci (multiple sequence alignment) je izvršeno između različitih tipova
ljudskog kolagena i između mišjeg kolagena koristeći SALIGN. Poravnanje
sekvenci fibrilarnih kolagena pokazalo je sličnosti u karakterističnim
glicinskim i prolinskim regijama te u mjestima na kojima su lizini u
sekvencama. Analiza aminokiselina pokazala je nekoliko razlika u sastavu
između različitih izvora kolagena.
Ekstrakcija kolagena iz meduza sastoji se od tri glavna koraka: pripreme
uzorka, ekstrakcije i oporavka, i može trajati do 7 dana. Ovaj dugotrajan
postupak može dovesti do degradacije kolagena i smanjenja konačnog
prinosa. Trenutne metode uključuju usitnjavanje tkiva i kemijsko tretiranje
tokom pripreme uzorka, kiselinsku i enzimsku ekstrakciju, kao i dijalizu. Cilj
ovog istraživanja bio je utvrditi kako promjene u koracima ekstrakcije utiču
na prinos, čistoću kolagena i trajanje metode. U tu svrhu, uzorci vrste
Rhizostoma pulmo su prvo liofilizirani, nakon čega je uslijedila ekstrakcija
kiselinom koristeći 0,5 M octenu kiselinu, isoljavanje pomoću NaCl i
pročišćavanje uz pomoć C18 kolona. Dobiveni kolagen je analiziran pomoću
MALDI-a kako bi se potvrdila uspješna ekstrakcija kolagena. Također, FTIR
i SDS-PAGE su odrađeni radi dodatne potvrde prisustva kolagena u izolatu.
Prinosi kolagena koji su dobiveni slični su onima zabilježenim u prethodnim
studijama. Na osnovu ovih rezultata, može se zaključiti da upotreba C18
kolona može biti alternativa za dijalizu i mogla bi skratiti proces ekstrakcije
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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