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Influence of diet and growth conditions on the carbon and nitrogen stable isotopic composition of Aspergillus niger Mycelium: insights for fungal chitosan characterization
Get PDFThis study investigates, for the first time, the relationship between carbon (δ 13C) and nitrogen stable isotopic composition of Aspergillus niger mycelium, used as chitin and chitosan sources, and the fungus diet under controlled cultivation conditions. Four diets were tested, combining different carbon (C3- and C4-glucose) and nitrogen sources (KNO3 and NH4Cl). Results showed that carbon sources significantly influenced δ 13C values of the mycelium: C4-glucose diets led to more negative ∆ 13C values (δ 13CMYCELIUM-δ 13CDIET) compared to C3-glucose diets. Nitrogen sources also affected isotopic fractionation, with KNO3 leading to negative ∆ 15N (δ 15NMYCELIUM-δ 15NDIET) and NH4Cl yielding positive ∆ 15N. Conversely, pH and temperature showed negligible effects on δ 15N, while continuous aeration during growth significantly decreased δ 15N, possibly due to partial assimilation of atmospheric nitrogen. These findings demonstrate that both nutrient and cultivation parameters can modulate the isotopic fractionation in A. niger, particularly for nitrogen. Although a direct correlation between diet composition and δ 15N could not be established, this work provides the first experimental link between fungal metabolism and its isotopic fingerprint. The results offer a scientific foundation for applying stable isotope ratio analysis to authenticate and trace fungal-derived chitin and chitosan, with potential applications in food and winemaking industries
Thermal patterns in a proglacial pond create Windows of Opportunity for periphyton growth (Cevedale glacier, Italy)
Get PDFIn high mountain areas, deglaciation is the most evident effect of anthropogenic climatic changes. Glacier retreat is inducing worldwide an increase of both number and size of proglacial lakes and ponds, i.e., lentic water bodies located in the proglacial area and directly linked to the glacier activity: the depressions carved in the land surface allow meltwater impoundment and accumulation of glacier sediment. Over the past decades, glacier-fed lakes have become an increasingly represented ecosystem in the Alpine landscape. However, their ecological characteristics are only partially known. Glacial runoff determines cascade effects in glacier-fed standing waters. It influences both water temperature, by delivering cold meltwater to the system, and water transparency, because of the high amount of inorganic suspended solids (so called “glacial flour”) that determine high water turbidity. Therefore, proglacial lakes are highly selective habitats, where planktonic communities are quantitatively scarce and taxonomically simplified. On the other hand, given the low input of allochthonous organic matter from bare proglacial forefields, benthic primary producers (periphyton) are the major autochthonous carbon source sustaining food webs in glacially fed water bodies. Studies on glacial streams show that periphyton growth is concentrated in “Windows of Opportunity” (WOs), mainly occurring in periods of reduced glacial runoff, i.e., autumn. In the euphotic zone along the littoral area of proglacial lakes, local conditions can allow algal growth (e.g., cyanobacteria), and the abrasive impact of glacial flour is low due to scarce water turbulence. Furthermore, in lentic proglacial ecosystems, periphyton ecological niches appear to be influenced also by water thermal stratification dynamics. Previous studies observed different mixing patterns in proglacial and clear mountain lakes (i.e., without glacial influence); high-altitude ponds (surface area < 2 ha) are expected to show even different patterns of response to physical environmental setting. To better understand the link between thermal dynamics and ecology of proglacial lentic systems, we investigated temperature dynamics in the water column of a proglacial pond located in the Eastern Italian Alps (South Tyrol, Italy) and compared them with density and taxonomic composition of PPNW 2024. Universitat de Girona (Spain) benthic diatom communities, which are key components of littoral periphyton in lakes and are useful bioindicators of environmental changes. Diatoms are eukaryotic photosynthetic microalgae, characterised by a cell wall composed of silica (frustule), whose morphological characteristics are used for taxonomical identification. Specific aims of the study were: (i) to characterise the temperature dynamics in a shallow proglacial pond; (ii) to investigate how thermal dynamics can influence the presence of WOs for periphyton growth. The proglacial pond is located at 2850 m a.s.l. in the Martell valley (Stelvio National Park, CE Italian Alps). It is moraine-dammed and originated from the retreat of the Cevedale glacier about 10 years ago. Its surface area is about 4270.5 m2 with a maximum depth of around 3 m. In the ice-free seasons 2022 and 2023, we installed water level sensors and performed water discharge measurements at the pond outlet with the salt dilution method, to build a flow rating curve and estimate outlet and inlet discharge time series. In summer 2023, we installed two buoys, one in the upstream part of the pond and one in the downstream part. Each buoy was equipped with 5 temperature sensors, located at 0, 0.2, 0.4, 0.8, 1.6 m depth, recording water temperature at 5-minute intervals. We applied the CE-QUAL-W2 model, a 2-D hydrodynamic laterally averaged model, to reconstruct the temperature time series in the water column for both ice-free seasons 2022 and 2023. We calibrated the parameters of the model based on field buoy data. Meteorological data and discharge time series were used as boundary conditions for the model. We developed a simplified numerical model to estimate the inflow water temperature (i.e., glacial runoff running on debriscovered ice) as a function of air temperature and solar radiation. Preliminary results show good agreement between the observed and modelled temperature data (RMSE < 1.5°C). During the Alpine glacial summer, we observed periods of pronounced daily thermal stratification. In these periods, shallow layers showed daily fluctuations, while deeper layers were colder. Total mixing and cooling of the water column followed intense precipitation events, with lower air temperature and solar radiation. In 2022 and 2023, we analysed the benthic diatom communities collected from a known area of colonised substrata (stones covered by a layer of consolidated sediment). In the laboratory, we eliminated the organic matter in the samples by chemical oxidation, to allow the morphological observation of diatom frustules. We equalised the sample volumes at 6 ml and added an aliquot (1 ml) of solution containing a known concentration of divinylbenzene microspheres, which served as reference to compare diatom densities in the different samples. Permanent diatom mounts were prepared, and diatom frustules and microspheres were counted under the optical microscopy. Diatom communities in the Cevedale proglacial pond reached higher density values (1-43 and 2.8-404.9 × 103 N valves/cm2, respectively) than in glacier-fed streams investigated in the same geographical area (Vulcano 2020, unpublished data). Moreover, we observed a density peak in August 2022 (404.9 × 103 N valves/cm2), and not in autumn as expected. In all samples, the community was numerically dominated by the pioneer species Achnanthidium minutissimum s.l. The observed diatom density patterns suggest that periphyton PPNW 2024. Universitat de Girona (Spain) growth in the proglacial pond can be sustained also in periods of high glacial runoff. Accordingly, the model results suggest the presence of additional temperature-driven WOs for periphyton growth during the Alpine summer, with respect to the ones described in glacier-fed streams. The presence and temporal extension of the WOs in the proglacial pond depend on meteorological conditions, as thermal gradients form during dry and warm periods. In a climate change perspective, this implies that colonisation processes in the periphyton of newly formed proglacial ponds may be accelerated by prolonged periods of drought, high air temperatures and increased glacial runoff. Consequently, the natural ecological evolution of proglacial ponds may be accelerated by global warming
Una Apis nulla Apis: le colonie selvatiche e la conservazione delle sottospecie e delle popolazioni locali
No full textEvidence of the relationship between the Oxygen Radical Absorbance Capacity (ORAC) and the potential exposure of white wines to Atypical Aging (ATA)
No full textAtypical aging (ATA) is a well-known wine defect that leads to unacceptable off-odors of wet mop, soap, waxy, furniture varnish, and dish rag. The main compound that is responsible for these undesired scents, 2-aminoacetophenone (AAP), results from the oxidative degradation of 3-indole acetic acid. This study aimed to investigate different technological factors for a possible effect on the oxygen radical absorption capacity (ORAC) and ATA development in white wine. Oxygenation during fermentation did not result in a significant effect on the development of ORAC or ATA development. Instead, the addition of ascorbic acid after vinification led to increased ORAC and decreased AAP formation. The first evidence of a tendency toward a correlation between ORAC and potential ATA development was found, with r = 0.365. This study showed the potential of the ORAC assay for an early assessment of the possible formation of ATA during wine storag
Predicting vegetables freshness perceived by consumers by using sensory and instrumental analysis
No full textMonoglucoside versus diglucoside anthocyanin evolution of red wine produced using a fungus-resistant grape cultivar (downy mildew and powdery mildew) under oxidative conditions
No full textThe need to reduce the use of pesticides in viticulture is increasing the interest in wines produced using fungal-resistant grapevine varieties, which are characterized by relevant contents of both monoglucoside and diglucoside anthocyanins. Aging in wooden barrels induces oxygen permeation into wine, but little is known about diglucoside anthocyanin evolution. Cabernet cortis wine was subjected to addition of oxygen and oak chips, and the anthocyanin changes were followed for 1 month. Decreases of 90% total monoglucosides, 80% acylated monoglucosides, 65% diglucosides, and 90% acylated diglucosides were observed. Monoglucosides formed pyranoanthocyanins, and the lower steric hindrance favored their polymerization with flavanols. Instead, the decrease in diglucosides was correlated to the number of hydroxyl groups of ring B, indicating the predominant oxidation of aglycones. However, three flavonol–anthocyanin–diglucoside derivatives named (epi)catechin-ethyl-Mv-dihexoside, (epi)catechin-ethyl-Pn-dihexoside, and (epi)catechin-Mv-dihexoside A-type were identified in wine for the first time. These research findings are useful for tuning suitable oenological practices to stabilize the color of these wines (type of barrel, aging times, oxygenation practices) and lower the malvin content, which currently is recommended by the OIV at a maximum of 15 mg/L and is a critical issue for their commercializatio
Il moscerino dei piccoli frutti Drosophila suzukii: l’insetto e il paesaggio, diffusione e gestione nel Nord Italia
Get PDFTaxonomic and functional metagenomic assessment of a Dolichospermum bloom in a large and deep lake south of the Alps
Get PDFUntargeted genetic approaches can be used to explore the high metabolic versatility of cyanobacteria. In this context, a comprehensive metagenomic shotgun analysis was performed on a population of Dolichospermum lemmermannii collected during a surface bloom in Lake Garda in the summer of 2020. Using a phylogenomic approach, the almost complete metagenome-assembled genome obtained from the analysis allowed to clarify the taxonomic position of the species within the genus Dolichospermum and contributed to frame the taxonomy of this genus within the ADA group (Anabaena/Dolichospermum/Aphanizomenon). In addition to common functional traits represented in the central metabolism of photosynthetic cyanobacteria, the genome annotation uncovered some distinctive and adaptive traits that helped define the factors that promote and maintain bloom-forming heterocytous nitrogen-fixing Nostocales in oligotrophic lakes. In addition, genetic clusters were identified that potentially encode several secondary metabolites that were previously unknown in the populations evolving in the southern Alpine Lake district. These included geosmin, anabaenopetins, and other bioactive compounds. The results expanded the knowledge of the distinctive competitive traits that drive algal blooms and provided guidance for more targeted analyses of cyanobacterial metabolites with implications for human health and water resource us