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Le Yondélis dans le traitement des sarcomes des tisuus mous. Etude de six cas patients à l'Institut Jean Godinot
No full textREIMS-BU Santé (514542104) / SudocSudocFranceF
Implantation immédiate post-extractionelle avec mise en charge en secteur antérieur unitaire (dernières évolutions)
No full textLYON1-BU Santé Odontologie (693882213) / SudocSudocFranceF
Les soins dentaires dans l'Antiquité gréco-romaine à travers six grands médecins
No full textREIMS-BU Santé (514542104) / SudocSudocFranceF
Caractérisation de nouvelles enzymes impliquées dans la biosynthèse de cofacteurs de microorganismes. Mécanismes des tyrosine lyases à radical SAM
No full textLe cofacteur F420 est un coenzyme d oxydoréduction essentiel pour la méthanogenèse chez les archées, un processus qui influence fortement les interactions métaboliques au sein du microbiote intestinal ; en outre, il joue un rôle important dans la pathogénicité de la bactérie Mycobacterium tuberculosis. L étude de sa biosynthèse présente donc un intérêt majeur en Biologie.La formation du chromophore du F420 est catalysée par la F0-synthase, qui contient, de façon unique, deux domaines caractéristiques des enzymes à radical SAM (rSAM). Ces enzymes catalysent le clivage de la S-adénosylméthionine (SAM) pour former un radical 5 déoxyadénosyle, capable d initier un grand nombre de réactions radicalaires.Nous avons réussi à identifier les substrats de la F0-synthase et à reconstituer la synthèse du F0 in vitro. Nous avons également démontré que cette enzyme contient deux centre [4Fe-4S] 2+/1+ rSAM fonctionnels et caractérisé les étapes de la synthèse du F0. Ceci nous a permis de proposer un mécanisme réactionnel pour la F0 synthase. Nous avons ensuite entrepris la comparaison de la F0 synthase avec les deux autres enzymes rSAM tyrosine lyases connues à ce jour : ThiH, impliquée dans la biosynthèse de la vitamine B1, et HydG, impliquée dans la biosynthèse du cofacteur métallique de l hydrogénase à fer-fer. Nous avons ainsi découvert de nouveaux aspects de la réaction de clivage de la tyrosine par ces enzymes, permettant une meilleure compréhension de ce groupe émergent au sein de la superfamille des enzymes rSAM.Cofactor F420 is a redox coenzyme crucial for methanogenesis in Archaea, a process which plays a major role in metabolic interactions in the gut microbiota ; It also constitutes a key pathogenicity factor for Mycobacterium tuberculosis. Understanding the biosynthesis of this cofactor is thus of major interest.The biosynthesis of the chromophore of F420 is catalyzed by F0 synthase, which comprises, in a unique manner, two radical SAM (rSAM) domains. These enzymes catalyze the cleavage of S adenosylmethionine (SAM) to produce a 5 -deoxyadenosyl radical, which can initiate a broad range of radical reactions.We succeeded to identify the substrates of F0-synthase and to perform the biosynthesis of F0 in vitro. We ascertained that F0-synthase contains two functional [4Fe-4S]2+/1+ rSAM clusters, and characterized the steps of the reaction of F0 synthesis. Based on these date, we proposed a mechanism for the F0-synthase reaction. Furthermore, we compared F0 synthase with the two other radical SAM tyrosine lyases identified to date: ThiH, which is involved in vitamin B1 biosynthesis, and HydG, which is involved in the biosynthesis of the metal cofactor of iron-iron hydrogenases. We obtained novel insights of the reaction of tyrosine cleavage catalyzed by these enzymes, providing a better understanding of this emerging group in the rSAM enzyme superfamily.PARIS11-SCD-Bib. électronique (914719901) / SudocSudocFranceF
La luminothérapie (apports possibles sur les désordres chronobiologiques en médecine de ville)
No full textRésumé françaisDIJON-BU Médecine Pharmacie (212312103) / SudocSudocFranceF
L' apnée (contraintes et adaptations physiologiques, conseils à l'officine)
No full textAIX-MARSEILLE2-BU Pharmacie (130552105) / SudocSudocFranceF
Règlementation cosmétique en 2013 et mise en conformité d'une société multinationale
No full textAIX-MARSEILLE2-BU Pharmacie (130552105) / SudocSudocFranceF
"Training for success?" : an anlaysis of the Irish Garda Siochana trainee programmes from 1922 to present day
No full textThe findings of a longitudinal, qualitative investigation of educating and training Irish trainees in a competency development framework and the meanings of training, and education and integration of both are explored. Themes' emerging from the analysis of in-depth interviews and surveys with police trainees, police and civilian teacher/trainers, police administrative and operational members suggests the induction programme for new entrants to the Irish . police is not achieving its intended development for trainees. Police trainees, police personnel and police and civilian teacher/trainers have their own views on the quality of the induction programme which do not always match that of the police organisations leadership policies, directives or mission statement. The findings inform on ways in which the accelerated recruitment campaign between 2004 and 2008 impacts on the quality of the education and training programme, initiatives and have implications for a wide range of policing services.EThOS - Electronic Theses Online ServiceGBUnited Kingdo
Mapping relational databases to semantic web using domain-specific knowledge
No full textEThOS - Electronic Theses Online ServiceGBUnited Kingdo
Molecular studies of plant-pathogen interactions in strawberry
No full textPowdery mildew is an important disease of crop plants. In strawberry, the disease is caused by the obligate biotrophic fungus Podosphoera ophonis (syn: Sphoeratheco mocu/oris f. sp. Fragorioe) and causes significant economic loss in the cultivated strawberry. This study used the diploid species Fragorio vesco as a model to study plant pathogen interactions. Initial studies involved in the identification of mildew resistance locus 0 (MLO) genes in the diploid species, F. vesco f. vesco and identified 17 FvMLO genes. Real-time quantitative PCR (qRT-PCR) results showed differential expression of 12 FvMLO genes in four strawberry varieties, F. vesco f. vesco (Fv), F. vesco ssp. vesco accession Hawaii 4 (HW), F. vesco f. semperf/orens line "Yellow Wonder 5AF7" (YW) and Eluica (octoploid strawberry). The FvML03 gene which is orthologous to the Arabidopsis AtML02, AtML06 and AtML012 and tomato S/ML01 genes required for powdery mildew susceptibility was highly expressed (164 fold) in YW compared to other FvMLO genes across varieties. The results showed that FvMLO genes can be used as potential candidates to engineer powdery mildew resistance in strawberry based on MLO suppression. To investigate the molecular mechanism underlying strawberry-powdery mildew interaction, RNA-seq was employed to generate a large transcriptome dataset in HW and YW at 1 d and 8 d after powdery mildew infection. We identified about 999 million (92%) reads mapped to the F. vesco genome. Transcripts were identified from a total of 23,470 and 23,464 genes in HW and YW, respectively at all three stages (control, 1DAI and 8DAI). Differential gene expression analysis identified 1,567, 1,846 and 1,145 upregulated genes between control and 1DAI, control and 8DAI, and 1DAI and ! lDAI, respectively in HW. Similarly, 1,336, 1,619 and 968 genes were upregulated in YW. Also 646, 1,098 and 624 down regulated genes were identified in HW, while 571, 754 and 627 genes were downregulated in YW between all three stages, respectively. The study also investigated differentially expressed genes (log2 fold changes <:5) between control and 1DAI in both HW and YW. A large number of genes related to secondary metabolism, signal transduction, transcriptional regulation and disease resistance were expressed predominantly either in HW or YW. These included flavonoid 3' -monooxygenase, peroxidase 15, glucan endo-1, 3-beta-glucosidase 2, receptor-like kinases, transcription factors, germin-like proteins, F-box proteins, NB-ARC and NBS-LRR proteins. This first application of RNA-seq to any pathogen interaction in strawberry provides wealth of genomic information for future studies in understanding molecular and cellular processes of the strawberry defence response to powdery mildew. It was also shown that metabolic lSN hydroponic isotope labelling of entire plants (HILEP) can be achieved in strawberry for quantitative proteomics analysis.EThOS - Electronic Theses Online ServiceGBUnited Kingdo