Publikationer från Uppsala Universitet
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Stability estimates for radial basis function methods applied to linear scalar conservation laws
No full textWe derive stability estimates for three commonly used radial basis function (RBF) methods to solve hyperbolic time-dependent PDEs: the RBF generated finite difference (RBF-FD) method, the RBF partition of unity method (RBF-PUM) and Kansa's (global) RBF method. We give the estimates in the discrete l(2)-norm 2-norm intrinsic to each of the three methods. The results show that Kansa's method and RBF-PUM can be l(2)-stable 2-stable in time under a sufficiently large oversampling of the discretized system of equations. The RBF-FD method in addition requires stabilization of the spurious jump terms due to the discontinuous RBF-FD cardinal basis functions. Numerical experiments show an agreement with our theoretical observations
Experiences and Fundamental Care Needs in Patients With Small Intestinal Neuroendocrine Tumours : An Interview Study in a Surgical Context
No full textAims The study aimed to describe patients' fundamental care needs and their experiences of nursing care, throughout surgical treatment of small intestinal neuroendocrine tumours. Design A qualitative descriptive study was performed. Methods Patients' interviews (n = 19) were conducted in Sweden from May 2021 to January 2022 and analysed using directed qualitative content analysis guided by the Fundamentals of Care framework. Results The results are presented in three descriptive categories chronologically throughout the care chain. In the preoperative phase of care, the category was ‘Feeling safe but lonely and frightened, and struggling with existential thoughts’; experiences in the postoperative phase of care resulted in the category ‘Feeling cared for but suffering from physical symptoms and feelings of loneliness’; and the category in the discharge phase was ‘Lacking self-care information and feeling worried about the future’. Conclusion There were deficiencies in the delivery of fundamental care for patients with a rare tumour diagnosis throughout surgical treatment. Nursing care is mostly task focused and fragmented, and there is a lack of psychosocial and relational care across the care chain. Registered nurses and nursing managers need to take responsibility for their leadership in nursing care to fulfil patients' fundamental care needs. The Fundamentals of Care framework could be used for work improvements to include all aspects of nursing care. Implications for the Profession and/or Patient Care High-quality nursing care is needed throughout the care chain, including self-care after discharge, for patients with this rare tumour diagnosis. A higher awareness of patients' experiences and the importance of psychosocial support is warranted. Registered nurses and nursing managers must revise and improve routines to support patients' psychosocial needs. Registered nurses need to take responsibility for their leadership in nursing care to fulfil patients' fundamental care needs. Impact What problem did the study address? This study highlights patients' fundamental care needs and experiences of nursing care throughout surgical treatment of small intestinal neuroendocrine tumours. What were the main findings? There are deficiencies in fulfilling patients' fundamental care needs across the care chain and in all dimensions of the Fundamental of Care framework throughout surgical treatment of small intestinal neuroendocrine tumours. Patients struggled with loneliness and existential thoughts, as well as worries about the future. Patients experienced a lack of information about plans for the day, self-care, and follow-ups. Where and on whom will the research have an impact? For clinicians to develop an understanding of, and improve, fundamental care needs for patients with small intestinal neuroendocrine tumours in a surgical context. For registered nurses to understand the importance of their leadership and nursing responsibility to fulfil fundamental care needs. Reporting Method The consolidated criteria for reporting qualitative research (COREQ): a 32-item checklist for interviews and focus groups. Patient Contribution The patients shared their experiences during the interviews, which has contributed to a deeper knowledge and understanding of the phenomena under study
Uncovering Sophisticated Discrimination with the Help of Credence Goods Markups : Evidence from a Natural Field Experiment
No full textCredence goods, such as repair and healthcare services, are characterized by profound information asymmetries between less-informed customers and better-informed expert sellers. These information asymmetries open the door for fraudulent behavior on the seller side. In a preregistered natural field experiment, we vary in one dimension the seller’s perception of whether the service is an ordinary or a credence good service and in the second dimension whether the customer is a member of a minority or a member of the majority. This allows us to measure the size of the induced credence goods markup and to address the question whether it interacts systematically with discrimination. We document the existence of a large credence goods markup, on average. Moreover, we find that members of the minority pay a sizeable discriminatory markup if the good is perceived as a credence good but not if it is perceived as an ordinary good. Our results show that sellers engage in sophisticated discrimination where informational asymmetries are used to hide discriminatory (fraudulent) behavior. With the help of an ex post survey, we derive a possible explanation for our results
Aurora kinase B is required for growth and expansion of medulloblastoma cells in the tissue context
No full textThe impact of the tissue context on tumor growth and drug response in medulloblastoma (MB) is poorly understood. To gain insights into the growth and dissemination behavior of the MB tumor cells under treatment, we combined three-dimensional cell culture screening with ex vivo organotypic cerebellum slice co-culture (OCSC), which allowed the assessment of tumor cell behavior in the tissue context. To identify druggable kinase pathways involved in invasion, we screened a panel of 274 kinase inhibitors and identified aurora kinase B (AURKB) as a potential anti-invasion drug target in MB. We validated tumor suppressive activities of the AURKB inhibitor (AURKBi) Barasertib (AZD1152-HQPA) and the structurally unrelated compound GSK-1070916 in cerebellum slice culture models for SHH, and Grp3 MB. Importantly, AURKBi are tumor suppressive in the tissue context, also in MB tumor cells that are in vitro resistant to the same treatment. We confirmed the requirement of AURKB for tumor growth and expansion in the tissue context through genetic suppression of AURKB by siRNA. We revealed that the combination of AURKBi with the SRC/BCR-ABL inhibitor Dasatinib acts synergistically to repress tumor growth and expansion in the highly invasive MB cell model ONS-76, but not in Grp3 MB cells. We demonstrate that tumor growth in the tissue context is suppressed by pharmacological inhibition of AURKB, comparable to the growth reduction observed after X-ray irradiation, which was used as the positive control. Finally, we show that exposure to µM concentrations of Barasertib does not cause developmental toxicity in fish larvae. In conclusion, we demonstrate that AURKB is essential for MB tumor growth and expansion in the tissue context and the inhibition of AURKB is equally efficient as irradiation in repressing tumor cell growth. In patients younger than three years, pharmacological targeting of AURKB may thus constitute a novel means to overcome radiotherapy limitations
Multiporate Poaceae pollen grains observed in the recent fossil record from the Greater Serengeti Ecosystem and Lake Victoria region
No full textThe analysis of fossil pollen from sediments is used to understand past vegetation and land cover variability. The observations of multiporate Poaceae pollen from sediments have received little attention in the literature and causes and rates of occurrence have few estimates, and the rates observed in the sediments are much lower than estimates observed from modern plants in Asia. Pollen analysis of the uppermost sediments from Speke Gulf, Lake Victoria, eastern Africa, showed relative abundances of Poaceae between 65 and 75% during the past centuries. A total of 19 of the ∼11,000 Poaceae pollen grains observed had conspicuous morphological variations and were documented. More consistent presence of abnormal grains occurred since the mid twentieth century, at the same time of increased anthropogenic environmental stressors. Multiporate pollen grains of Poaceae have been previously observed in Asia, South America, and northern Africa, predominantly in the Panicoideae subfamily. Morphological variations may present an added challenge for automated pollen identification techniques and descriptions of fossil pollen
Development of an SPH-based numerical wave-current tank and application to wave energy converters
No full textThis research proposes a high-fidelity based numerical tank designed to analyze the modified hydrodynamics that develops in waves-current fields, aimed at generating power matrices for wave energy converters (WEC). This tank is developed within the open source DualSPHysics Lagrangian framework using the Smoothed Particle Hydrodynamics (SPH) method, validated with physical data, and applied to simulate a point-absorber WEC. Our proposed numerical facility implements open boundary conditions, employing third-order consistent wave theory for direct generation, with flow field constrained by a Doppler correlation function. Reference data is collected from dedicated physical tests for monochromatic waves; the wave-current numerical basin demonstrates very high accuracy in terms of wave transformation and velocity field. In the second segment of this paper, a current-aware power transfer function is computed for the taut-moored point-absorber Uppsala University WEC (UUWEC). Parametrically defined regular waves with uniform currents are utilized to map an operational sea state featuring currents of different directions and intensities. In terms of power capture capabilities, the modified dynamics observed in presence of currents translates in a dependence of the WEC's power matrix not only on wave parameters, but also on current layouts. The UUWEC's power output has revealed that regardless of current directionality, annual output consistently decreases, with a registered power drop as high as 10% when an expected current field is introduced
Y-chromosomal insights into the breeding history and sire line genealogies of two traditional Baroque horse breeds : Lipizzaner and Kladruber
No full textThe paternally inherited, male-specific part of the Y chromosome (MSY) is an ideal marker for studying the origin, genealogies, and historical connections of horse patrilines. Here, we performed fine-scaled MSY haplotype (HT) analysis in two Baroque horse breeds, the Lipizzaner and the Kladruber, both known for their long-standing tradition of sire line breeding and interconnected genealogies. We genotyped 95 MSY markers using KASPTM technology in 90 stallions representing all patrilines of both breeds. We identified 14 HTs across eight Lipizzaner sire lines and six HTs in eight Kladruber lines. Y-chromosomal analysis confirmed the presumed Arabian and Iberian origins of two Lipizzaner and two Kladruber foundation sires. Interestingly, six Lipizzaner sire lines clustered into the MSY haplogroup (HG) daC_Tb, a HG previously associated with the Turkoman horse, suggesting a Turkish origin of several Lipizzaner foundation sires, contrary to documented records. We also found evidence for an undocumented Arabian founder in the Lipizzaner breed. Furthermore, three private HTs were detected in Kladruber horses, highlighting the uniqueness of their foundation sires. HTs in shared patrilines between Lipizzaner and Kladruber were consistent, while three Lipizzaner sublines showed discrepancies between MSY results and recorded pedigree data. In conclusion, MSY haplotyping validated historical breed documentation, revealed new insights into the origins and distribution of sire lines, and proved effective in resolving parentage issues across generations
“….det är fantastiskt, när man använder det rätt” : En intervju- och observationsstudie om användning av surfplattor och digitala verktyg i förskoleverksamheter
No full textSymplectic Cuts and Open/Closed Strings I
No full textThis paper introduces a concrete relation between genus zero closed Gromov-Witten invariants of Calabi-Yau threefolds and genus zero open Gromov-Witten invariants of a Lagrangian A-brane in the same threefold. Symplectic cutting is a natural operation that decomposes a symplectic manifold (X,ω) with a Hamiltonian U(1) action into two pieces glued along an invariant divisor. In this paper we study a quantum uplift of the cut construction defined in terms of equivariant gauged linear sigma models. The nexus between closed and open Gromov-Witten invariants is a quantum Lebesgue measure associated to a choice of cut, that we introduce and study. Integration of this measure recovers the equivariant quantum volume of the whole CY3, thereby encoding closed Gromov-Witten invariants. Conversely, the monodromies of the quantum measure around cycles in Kähler moduli space encode open Gromov-Witten invariants of a Lagrangian A-brane associated to the cut. Both in the closed and the open string sector we find a remarkable interplay between worldsheet instantons and semiclassical volumes regularized by equivariance. This leads to equivariant generating functions of GW invariants that extend smoothly across the entire moduli space, and which provide a unifying description of standard GW potentials. The latter are recovered in the non-equivariant limit in each of the different phases of the geometry
Sharing of cmeRABC alleles between C. coli and C. jejuni associated with extensive drug resistance in Campylobacter isolates from infants and poultry in the Peruvian Amazon
No full textCampylobacter is a serious health threat because of the rapid progressive evolution of antimicrobial resistance and efficient transmission from zoonotic as well as human sources. Resistance to fluoroquinolones and macrolides is particularly concerning as this compromises the two most effective oral antibiotic agents currently available for human campylobacteriosis. Here, we report on the prevalence and worldwide distribution of the operon cmeRABC, which encodes an efflux pump conferring high levels of combined resistance to fluoroquinolones and macrolides in Campylobacter strains isolated from poultry (n = 75) and children (n = 177). These mutations were found to be highly prevalent in isolates from poultry (62.7%) and children (29.4%) in Iquitos, Peru. We investigated the population structure of genes in the cmeRABC operon and identified a potential genetic bottleneck for the cmeA and cmeB genes. While most cmeB alleles segregate by species, alleles associated with high resistance to fluoroquinolones and macrolides were found in both Campylobacter jejuni and Campylobacter coli. We inferred that the likely ancestry of these alleles was from C. jejuni and was later acquired by C. coli through recombination. Publicly accessible global genomic data from 16,120 Campylobacter genomes identified these mutations in approximately 6% of C. jejuni and C. coli isolates globally, with higher prevalence in samples from poultry in many countries, including Peru. Our findings suggest that these extensively drug-resistant Campylobacter strains originated from C. jejuni in poultry