19683 research outputs found
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Prophylactic and therapeutic potential of vitamin D in asthma during the COVID-19 pandemic: the new hope?
No full textOver the last two decades, the emergence of lethal virulent strains of coronavirus (CoV), including the severe acute respiratory syndrome CoV 2 (SARS-CoV-2), which is responsible for the coronavirus disease 2019 (COVID-19) pandemic, has become a matter of great attention to the scientific community. Despite the implementation of preventive measures throughout the world, the spread of this disease and associated co-morbidities and mortality continue in all countries, continents, and populations of all ages. COVID-19 is highly contagious. Clinical manifestations are diverse and range from asymptomatic, mild to severe, life-threatening complications in the elderly and patients with underlying conditions such as cardiovascular disease, diabetes, obesity, and asthma. In addition, viral infections can trigger asthma attacks. To date, there is no specific treatment schema to combat COVID-19 disease. Current patient care revolves around disease severity and supportive treatment of symptoms from home-rest in mild disease to anti-viral therapy, oxygen support, anti-inflammatories, and anti-coagulants in severe COVID-19. Regarding prevention, the World Health Organization recommends vaccination, social distancing, quarantine, the wearing of surgical masks, and handwashing. In many countries, vaccination is optional, and given that parents are often reluctant to vaccinate themselves and their children for fear of side effects, identifying ways to enhance or support the immune system to prevent infection or improve recovery in vulnerable populations is worth investigating. Furthermore, research has focused on the pharmacological management of COVID-19 symptoms and much less has been published on nutrition therapy. Therefore, the scope of this review is to summarize the latest evidence on the use of vitamin D to support the metabolism and the immune system of asthma patients during the COVID-19 pandemic. A brief overview of asthma and COVID-19 pathophysiology, COVID-19 treatment guidelines for asthma patients, and the role of vitamin D in lung health, including the optimal blood level required to enhance immunity, will be suggested
Assessment of the Guardianship System for Persons with Psychosocial Disability in Indonesia
No full textThis article examines Indonesia’s guardianship system for persons with psychosocial disabilities (PPDs) following the country’s ratification of the Convention on the Rights of Persons with Disabilities (CRPD). Despite this ratification, Indonesia continues using a substitute decision-making framework, violating CRPD principles. Analyzing 49 court decisions from 2015 to 2018, the study identifies issues such as outdated criteria for guardianship, inappropriate involvement of religious courts, and the use of insufficient evidence during hearings. Focus group discussions with PPDs, caregivers, and experts reveal fears and hardships faced under the current system. The findings indicate most applications are granted without thorough consideration, often based on inadequate evidence. The article calls for legal reforms to align with CRPD mandates, advocating for a supported decision-making framework to protect PPDs’ rights and autonomy. Indonesia must adopt a contemporary understanding of capacity to ensure PPDs retain their legal capacity and receive necessary support
Effect of Data-Processing Methods on Acceleration Summary Metrics of GNSS Devices in Elite Australian Football
No full textThis study aimed to measure the differences in commonly used summary acceleration metrics during elite Australian football games under three different data processing protocols (raw, custom-processed, manufacturer-processed). Estimates of distance, speed and acceleration were collected with a 10-Hz GNSS tracking technology device from fourteen matches of 38 elite Australian football players from one team. Raw and manufacturer-processed data were exported from respective proprietary software and two common summary acceleration metrics (number of efforts and distance within medium/high-intensity zone) were calculated for the three processing methods. To estimate the effect of the three different data processing methods on the summary metrics, linear mixed models were used. The main findings demonstrated that there were substantial differences between the three processing methods; the manufacturer-processed acceleration data had the lowest reported distance (up to 184 times lower) and efforts (up to 89 times lower), followed by the custom-processed distance (up to 3.3 times lower) and efforts (up to 4.3 times lower), where raw data had the highest reported distance and efforts. The results indicated that different processing methods changed the metric output and in turn alters the quantification of the demands of a sport (volume, intensity and frequency of the metrics). Coaches, practitioners and researchers need to understand that various processing methods alter the summary metrics of acceleration data. By being informed about how these metrics are affected by processing methods, they can better interpret the data available and effectively tailor their training programs to match the demands of competition
Two-Layered Multi-Factor Authentication Using Decentralized Blockchain in an IoT Environment
No full textInternet of Things (IoT) technology is evolving over the peak of smart infrastructure with the participation of IoT devices in a wide range of applications. Traditional IoT authentication methods are vulnerable to threats due to wireless data transmission. However, IoT devices are resource- and energy-constrained, so building lightweight security that provides stronger authentication is essential. This paper proposes a novel, two-layered multi-factor authentication (2L-MFA) framework using blockchain to enhance IoT devices and user security. The first level of authentication is for IoT devices, one that considers secret keys, geographical location, and physically unclonable function (PUF). Proof-of-authentication (PoAh) and elliptic curve Diffie–Hellman are followed for lightweight and low latency support. Second-level authentication for IoT users, which are sub-categorized into four levels, each defined by specific factors such as identity, password, and biometrics. The first level involves a matrix-based password; the second level utilizes the elliptic curve digital signature algorithm (ECDSA); and levels 3 and 4 are secured with iris and finger vein, providing comprehensive and robust authentication. We deployed fuzzy logic to validate the authentication and make the system more robust. The 2L-MFA model significantly improves performance, reducing registration, login, and authentication times by up to 25%, 50%, and 25%, respectively, facilitating quicker cloud access post-authentication and enhancing overall efficiency
Resolving Nonequilibrium Shape Variations among Millions of Gold Nanoparticles
No full textNanoparticles, exhibiting functionally relevant structural heterogeneity, are at the forefront of cutting-edge research. Now, high-throughput single-particle imaging (SPI) with X-ray free-electron lasers (XFELs) creates opportunities for recovering the shape distributions of millions of particles that exhibit functionally relevant structural heterogeneity. To realize this potential, three challenges have to be overcome: (1) simultaneous parametrization of structural variability in real and reciprocal spaces; (2) efficiently inferring the latent parameters of each SPI measurement; (3) scaling up comparisons between 105 structural models and 106 XFEL-SPI measurements. Here, we describe how we overcame these three challenges to resolve the nonequilibrium shape distributions within millions of gold nanoparticles imaged at the European XFEL. These shape distributions allowed us to quantify the degree of asymmetry in these particles, discover a relatively stable “shape envelope” among nanoparticles, discern finite-size effects related to shape-controlling surfactants, and extrapolate nanoparticles’ shapes to their idealized thermodynamic limit. Ultimately, these demonstrations show that XFEL SPI can help transform nanoparticle shape characterization from anecdotally interesting to statistically meaningful
Zinc mediates control of nitrogen fixation via transcription factor filamentation
No full textAbstract: Plants adapt to fluctuating environmental conditions by adjusting their metabolism and gene expression to maintain fitness. In legumes, nitrogen homeostasis is maintained by balancing nitrogen acquired from soil resources with nitrogen fixation by symbiotic bacteria in root nodules. Here we show that zinc, an essential plant micronutrient, acts as an intracellular second messenger that connects environmental changes to transcription factor control of metabolic activity in root nodules. We identify a transcriptional regulator, FIXATION UNDER NITRATE (FUN), which acts as a sensor, with zinc controlling the transition between an inactive filamentous megastructure and an active transcriptional regulator. Lower zinc concentrations in the nodule, which we show occur in response to higher levels of soil nitrate, dissociates the filament and activates FUN. FUN then directly targets multiple pathways to initiate breakdown of the nodule. The zinc-dependent filamentation mechanism thus establishes a concentration readout to adapt nodule function to the environmental nitrogen conditions. In a wider perspective, these results have implications for understanding the roles of metal ions in integration of environmental signals with plant development and optimizing delivery of fixed nitrogen in legume crops.</p
Navigating tensions in climate change-related planned relocation
No full textThe planned relocation of communities away from areas of climate-related risk has emerged as a critical strategy to adapt to the impacts of climate change. Empirical examples from around the world show, however, that such relocations often lead to poor outcomes for affected communities. To address this challenge, and contribute to developing guidelines for just and sustainable relocation processes, this paper calls attention to three fundamental tensions in planned relocation processes: (1) conceptualizations of risk and habitability; (2) community consultation and ownership; and (3) siloed policy frameworks and funding mechanisms. Drawing on the collective experience of 29 researchers, policymakers and practitioners from around the world working on planned relocations in the context of a changing climate, we provide strategies for collectively and collaboratively acknowledging and navigating these tensions among actors at all levels, to foster more equitable and sustainable relocation processes and outcomes
Exploring the Experiences of Regional and Rural Revictimized Women in a Group Empowerment Program
No full textSexual revictimization can have a negative impact on many facets of women’s wellbeing, yet limited evidence exists regarding specific interventions that support healing and the reduction of further revictimization. This paper will explore regional and rural women’s experience of a group-based empowerment program, the Shark Cage program, in Victoria, Australia. The “Shark Cage” program aims to address revictimization by empowering women and girls to build personal boundaries and assertiveness within the context of gender equality and human rights. Data were collected via participant observations across the 8-week program, in combination with semi-structured interviews with participants (N = 11) pre and post intervention. All participants had access to therapeutic support outside of the program. Findings indicate that the program fostered connections among women with shared experiences of sexual revictimization, reducing feelings of isolation. Participants detailed the benefit of developing and practicing skills in reducing revictimization, such as assertiveness and boundary setting. Program learning and recovery was embedded within a network of embodied emotions, social connections, cultural norms and place-based relations that influenced how participants recovery could be understood, processed and addressed
1H NMR spectroscopic characterisation of HepG2 cells as a model metabolic system for toxicology studies
No full textThe immortalised human hepatocellular HepG2 cell line is commonly used for toxicology studies as an alternative to animal testing due to its characteristic liver-distinctive functions. However, little is known about the baseline metabolic changes within these cells upon toxin exposure. We have applied 1H Nuclear Magnetic Resonance (NMR) spectroscopy to characterise the biochemical composition of HepG2 cells at baseline and post-exposure to hydrogen peroxide (H2O2). Metabolic profiles of live cells, cell extracts, and their spent media supernatants were obtained using 1H high-resolution magic angle spinning (HR-MAS) NMR and 1H NMR spectroscopic techniques. Orthogonal partial least squares discriminant analysis (O-PLS-DA) was used to characterise the metabolites that differed between the baseline and H2O2 treated groups. The results showed that H2O2 caused alterations to 10 metabolites, including acetate, glutamate, lipids, phosphocholine, and creatine in the live cells; 25 metabolites, including acetate, alanine, adenosine diphosphate (ADP), aspartate, citrate, creatine, glucose, glutamine, glutathione, and lactate in the cell extracts, and 22 metabolites, including acetate, alanine, formate, glucose, pyruvate, phenylalanine, threonine, tryptophan, tyrosine, and valine in the cell supernatants. At least 10 biochemical pathways associated with these metabolites were disrupted upon toxin exposure, including those involved in energy, lipid, and amino acid metabolism. Our findings illustrate the ability of NMR-based metabolic profiling of immortalised human cells to detect metabolic effects on central metabolism due to toxin exposure. The established data sets will enable more subtle biochemical changes in the HepG2 model cell system to be identified in future toxicity testing
Resilience to summer bushfire in the threatened orchid, Caladenia tessellata, in terms of pollination success, herbivory, and mycorrhizal associations
No full textIn some biogeographic regions, many threatened plant species occur in habitats that periodically experience bushfire. However, we currently have relatively little information on how important plant-animal and plant-fungus interactions are affected by these fires. For the threatened sexually deceptive orchid Caladenia tessellata we test whether pollination rate, frequency of florivory, and the species of mycorrhizae the plant associates with differ between burnt and unburnt sites. Interestingly, pollination rates were unaffected by fire, demonstrating that populations of the thynnine wasp pollinator can persist post-fire. However, there was a significant negative relationship between number of flowers in a population and pollination success, which is likely a by-product of a deceptive pollination strategy. Despite the presence of vertebrate herbivores, florivory rates were low in both burnt and unburnt sites. Caladenia tessellata associated primarily with the mycorrhiza Serendiptia australiana, regardless of fire history. While our results suggest resilience to a one-off summer fire for the ecological interactions that we measured, it would be interesting to investigate the effects of fire frequency and time of year. High reproductive rates in small populations of C. tessellata suggest these populations may be viable and that retaining them is a high priority for conservation