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Early Software Defects Density Prediction: Training the International Software Benchmarking Cross Projects Data Using Supervised Learning
No full text"You're Going to Go into Some Really Dark, Dark Places in Your Mind." Loss and Disillusionment of Being Shunned From the Jehovah's Witnesses Community and Its Impact
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Serum concentration impacts myosin heavy chain expression but not cellular respiration in human LHCN-M2 myoblasts undergoing differentiation
No full textNEW FINDINGS: What is the central question of this study? Does the concentration of human serum affect skeletal muscle differentiation and cellular respiration of LHCN-M2 myoblasts? What is the main finding and its importance? The concentration of serum used to differentiate LHCN-M2 skeletal muscle cells impacts the coverage of myosin heavy chain, a marker of terminally differentiated myotubes. Normalisation of mitochondrial function data to total protein negates the differences observed in absolute values, which differ as a result of increased protein content when differentiation occurs with increasing concentration of serum.ABSTRACT: The human LHCN-M2 myoblast cell line has the potential to be used to investigate skeletal muscle development and metabolism. Experiments were performed to determine how different concentrations of human serum affect myogenic differentiation and mitochondrial function of LHCN-M2 cells. LHCN-M2 myoblasts were differentiated in serum-free medium, 0.5% or 2% human serum for 5 and 10 days. Myotube formation was assessed by immunofluorescence staining of myosin heavy chain (MHC) and molecularly by mRNA expression of Myogenic differentiation 1 (MYOD1) and Myoregulatory factor 5 (MYF5). Following differentiation, mitochondrial function was assessed to establish the impact of serum concentration on mitochondrial function. Time in differentiation increased mRNA expression of MYOD1 (day 5, 6.58 ± 1.33-fold; and day 10, 4.28 ± 1.71-fold) (P = 0.012), while suppressing the expression of MYF5 (day 5, 0.21 ± 0.11-fold; and day 10, 0.06 ± 0.03-fold) (P = 0.001), regardless of the serum concentration. Higher serum concentrations increased MHC area (serum free, 11.92 ± 0.85%; 0.5%, 23.10 ± 5.82%; 2%, 43.94 ± 8.92%) (P = 0.001). Absolute basal respiration approached significance (P = 0.06) with significant differences in baseline oxygen consumption rate (P = 0.025) and proton leak (P = 0.006) when differentiated in 2% human serum, but these were not different between conditions when normalised to total protein. Our findings show that increasing concentrations of serum of LHCN-M2 skeletal muscle cells into multinucleated myotubes, but this does not affect relative mitochondrial function.</p
Spectral and cross-spectral analysis—A tutorial for psychologists and social scientists.
No full textA Genetic Algorithm-Based Virtual Machine Allocation Policy for Load Balancing Using Actual Asymmetric Workload Traces
No full textUnderstanding the Rights of Nature: Working Together Across and Beyond Disciplines
No full textRecognising the rights of nature is seen by many as the paradigm shift needed to truly embed ecology and the environment into nature-based policy and management solutions to address biodiversity loss, climate change, and sustainable development. However, despite its potential, research across and beyond disciplinary boundaries remains very limited, with most located in the humanities and social sciences and often lacking connection with environmental sciences. Based on a multi-, inter-, and transdisciplinary project, we identify some critical common themes among the humanities, social sciences, and environmental sciences to support future research on the potential of the rights of nature to address contemporary social-environmental challenges. We argue that future research needs to be not only interdisciplinary but also transdisciplinary since the movement of rights of nature is often driven by and based on knowledge emerging outside of academic disciplines
Relationships, technology and the role of living arrangements during social lockdown related to COVID-19
Get PDF© 2023,Bristol University Press. This is an author produced version of a paper published in Families, Relationships and Societies uploaded in accordance with the publisher’s self- archiving policy. The final published version (version of record) is available online at the link. Some minor differences between this version and the final published version may remain. We suggest you refer to the final published version should you wish to cite from it. COVID-19-related social lockdowns had profound consequences in all aspects of social life, yet the role of technology to mediate relationships during lockdown has received little attention. Drawing on a survey of 565 young adults in the UK, we used mixed methods to explore differences in technology use by people in serious romantic relationships (co-habiting vs. living apart together), casual relationships, or single and (b) how COVID-19 influenced long-term, serious relationships. For participants in a serious relationship, technology was used as a strategy to facilitate ongoing communication, enabling partners to achieve “intimacy from afar.” Qualitative analysis revealed five reasons (e.g., more free time, navigating lockdown restrictions, greater boredom, desire for love, miscellaneous) for changes in online dating profile usage. People in serious relationships perceived deeper intimate bonds, boundary issues, less physical intimacy, difficulty with lockdown separation, and greater negative impact as a result of COVID-19. Limitations and implications are discussed