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Metabolism of 2,2ʼ,3,4,4ʼ,5,6ʼ-Heptachlorobiphenyl (CB182) by Rat, Guinea Pig and Human Liver Microsomes
No full textThe in vitro metabolism of 2,2ʼ,3,4,4ʼ,5,6ʼ-heptachlorobiphenyl (CB182) by rat, guinea pig and human liver microsomes was compared and the effects of cytochrome P450 (CYP) inducers, phenobarbital (PB) and 3-methylcholanthrene (MC), on CB182 metabolism were examined. Only one metabolite was produced by rat, guinea pig and human liver microsomes and the order of the activity was rat (PB-treated)＞＞ guinea pig (PB-treated)＞ guinea pig (untreated, MC-treated)＞ human ＞ rat (untreated). Pretreatment of PB resulted in the remarkable increase of the metabolite in rats (1,370 pmol/hr/mg protein) and a slight increase in guinea pigs (27 pmol/hr/mg protein). In contrast, MC treatment to rats and guinea pigs decreased M-1. By comparison of GC-MS data of the methylated M-1 with a synthesized authentic sample, M-1 was determined to be 3ʼ-hydroxy (OH)-CB182. These results suggest that 3ʼ-OH-CB182 is a major metabolite formed by PB-inducible CYP2B enzymes in both animals and rat CYP2B enzymes possess much higher activity to hydroxylate CB182 than guinea pig and human CYP2B enzymes
Inter-Laboratory Cross-Comparison Study of Dioxins and PCBs Analyses in Human Blood Samples (Part 2)
No full textWe developed an analytical method for accurately determining the concentrations of dioxins and polychlorinated biphenyls (PCBs) in a blood volume of 5 g. Inter-laboratory cross-comparison is available for maintaining the quality of dioxins and PCBs analyses. We carried out the cross-comparison of dioxin and PCBs analyses in the blood with domestic analysis organizations. Two cross-comparison studies each of dioxins and PCBs analyses were carried out from 2012 through 2015. As a result, the findings of our analysis accorded well with the analyses of the other organizations. It was found that our analytical method is sufficiently reliable
現代中国の著名作家 冰心(謝冰心)の自筆完全原稿を発見
No full text現代中国の著名作家 冰心(謝冰心)の自筆完全原稿を発見 : 九州大学広報室記者発表会 : 2017年6月16日 : 九州大学附属図書館Kyushu University Press Release : Tuesday, June 20, 2017 : Kyushu Univesity Library, Fukuoka, Japa
Thyroid Function in Patients with Yusho
No full textTo investigate chronic effects of polychlorinated biphenyls (PCBs) and polychlorinated dibenzofurans (PCDFs) on thyroid functions, serum concentrations of thyroid stimulating hormone (TSH), triiodothyronine (T_3), thyroxine (T_4) and free T_4 were studied in serum of 197 patients with Yusho and 42 control subjects in 2015. Serum levels of T_3 were decreased in patients with Yusho compared with controls. A significant relationship between concentrations of PCB in the blood and serum levels of T_3 was observed, while there was no correlation between blood PCB concentrations and TSH, T_4 or free T_4 levels. Serum levels of T_3 were significantly decreased in the subjects with high PCB concentrations compared with those with low PCB concentrations. Furthermore, serum levels of T_3 were significantly decreased in the subjects with high 2,3,4,7,8-pentachlorodibenzofuran (PeCDF) concentrations compared with those with low 2,3,4,7,8-PeCDF concentrations. We conclude that serum T_3 levels decrease in patients with Yusho, and it may be associated with PCBs and 2,3,4,7,8-PeCDF in the blood
Peripheral Membrane Interactions Boost the Engagement by an Anti-HIV-1 Broadly Neutralizing Antibody.
No full textThe 4E10 antibody displays an extreme breadth of HIV-1 neutralization and therefore constitutes a suitable model system for structure-guided vaccine design and immunotherapeutics against AIDS. In this regard, the relevance of autoreactivity with membrane lipids for the biological function of this antibody is still a subject of controversy. To address this dispute, herein we have compared the membrane partitioning ability of the 4E10 antibody and several of its variants, which were mutated at the region of the paratope surface in contact with the membrane interface. We first employed a physical separation approach (vesicle flotation) and subsequently carried out quantitative fluorescence measurements in an intact system (spectroscopic titration), using 4E10 Fab labeled with a polarity-sensitive fluorescent probe. Moreover, recognition of epitope peptide in membrane was demonstrated by photo-cross-linking assays using a Fab that incorporated the genetically encoded unnatural amino acid p-benzoylphenylalanine. The experimental data ruled out that the proposed stereospecific recognition of viral lipids was necessary for the function of the antibody. In contrast, our data suggest that nonspecific electrostatic interactions between basic residues of 4E10 and acidic phospholipids in the membranes contribute to the observed biological function. Moreover, the energetics of membrane partitioning indicated that 4E10 behaves as a peripheral membrane protein, tightening the binding to the ligand epitope inserted in the viral membrane. The implications of these findings for the natural production and biological function of this antibody are discussed
Development of junction temperature measurement system for blue LED in operation using pulsed-laser Raman scattering
No full textA novel method by using a pulsed-laser Raman scattering technique was employed to estimate junction temperatures of a phosphor-less blue-LED module. Observation of GaN-E_2^H Raman spectra was done successfully and temperature dependent Raman spectra of GaN were detected. This technique was applied to simultaneous estimation of the junction temperatures of the several chips in the phosphor-less blue LED module in operation. It is concluded that this technique would be a useful method for remoteness and simultaneous multipoint measurement of the junction temperature of a LED module in which several chips are integrated
Recent Real Estate Transactions under Easing Monetary Policy
No full text1.はじめに 2.近年の金融・経済情勢 3.経済活動と不動産価格 4.不動産価格の変動に関する検証 5.経済活動と不動産取
日本十進分類法新訂10版の概要
No full text1.10版刊行までの経緯 2.10版の外観 3.10版の構成 4.10版の内容 5.10版の今後平成28年度国立大学図書館協会地区協会助成事業九州地区講演会「2020年の目録システムは？」 : 主催:九州地区国立大学図書館協会 共催:九州大学附属図書館 後援:九州地区大学図書館協議会 : 平成29年3月10日(金)13:00～17:00 : 九州大学（箱崎）中央図書館 4F視聴覚ホー
CAT2020 : NACSIS-CAT検討作業部会の検討状況について
No full text1.NACSIS-CAT概要 2.NACSIS-CAT再構築についての検討経緯 3.NACSIS-CAT検討作業部会について 4.CAT2020のポイント 5.NACSIS-CAT詳細案 6.今後に向けて平成28年度国立大学図書館協会地区協会助成事業九州地区講演会「2020年の目録システムは？」 : 主催:九州地区国立大学図書館協会 共催:九州大学附属図書館 後援:九州地区大学図書館協議会 : 平成29年3月10日(金)13:00～17:00 : 九州大学（箱崎）中央図書館 4F視聴覚ホー