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The hepatic phenotype of Niemann-Pick type C disease exhibits stard1 upregulation independently of endoplasmic reticulum stress
No full textTrabajo presentado en AASLD - The Liver Meeting 2019, celebrado en Boston, Massachusetts (Estados Unidos), del 8 al 12 de noviembre de 2019Niemann-Pick type C (NPC) disease is
an inherited lysosomal storage disorder that results in
neurodegeneration and liver disease The hepatic phenotype
can result in liver failure and premature death preceding
the neurodegenerative symptoms Due to defective NPC1
function, the primary biochemical feature of NPC disease
translates in lysosomal cholesterol and glycosphingolipids
accumulation Moreover, mitochondrial cholesterol (mChol)
accumulation and GSH depletion have been reported as
well, but the underlying mechanism remains unknown
MLN64 (StARD3) provides cholesterol to the mitochondrial
outer membrane from endosomes, while StARD1 plays a
crucial role in the trafficking of cholesterol to mitochondrial
inner membrane. Previous findings showed that endoplasmic
reticulum (ER) stress contributes to the upregulation of
StARD1 Furthermore, acid ceramidase (ACDase), an
enzyme that regulates ceramide homeostasis, has been
shown to repress StARD1 expression through binding to the
nuclear transcription steroidogenic factor SF¿1 Therefore,
our aim was to investigate the mechanisms underlying
StARD1 upregulation in NPC disease and if this effect is
regulated by ACDas
Stop Motion XSCAPE Project. Material Minds Lab. INCIPIT-CSIC
No full textGuion: Silvana Cabrera-Guillen, Sara Lede-Caamaño; Fotos y edición: Rubén Vuelta-Santín, Servicio de Medios Audiovisuales del INCIPIT-CSIC; Música: The Hardest Button to Button - The White Stripes.Vídeo stop motion promocional para redes sociales, sobre los experimentos de percepción visual del proyecto XSCAPE, realizados en el Material Minds Lab del INCIPIT-CSIC.Peer reviewe
Enhancing Soil Carbon Sequestration with C-Rich Carrier Materials from Spent Mushroom Substrate and Composted Wheat Straw: Implications for Smart Fertilizer Design
Get PDF16 Páginas.-- 6 Figuras.-- 4 TablasThis study aimed to evaluate the potential of two carbon (C)-rich carrier materials derived from agricultural residues, spent mushroom substrate (SMS) and composted wheat straw (CWS) for enhancing soil carbon stabilization in an Andisol, with a view towards their future application in smart fertilizer design. We investigated how their contrasting nitrogen contents and application rates affect soil organic carbon dynamics and contribute to sustainable soil management and climate change mitigation. Soil incubations were conducted over 365 days, during which C mineralization, enzymatic activities, and C and N sequestration were assessed. SMS addition at both low and high rates (0.5% and 1% soil C increase, respectively) enhanced soil organic carbon (SOC) stabilization, increasing mean residence times (40.6 and 48.8 years) and half-life times (28.1 and 33.8 years) of the stable C pool compared to unamended soil (35.9 and 24.9 years). High-rate CWS application (1% soil C increase) promoted native SOC decomposition, increasing C losses (5.8%) and reducing C sequestration potential (96%). However, low-rate CWS application (0.5% soil C increase) showed promise, increasing mean residence time (46.8 years) and half-life time (32.4 years) of the stable C pool. Spearman correlations revealed positive associations between electrical conductivity, total N, humification indices, and C stabilization parameters, highlighting the importance of nutrient availability and humification potential for C stabilization. Incorporating C-rich carrier materials with balanced nutrient content, such as SMS, can enhance soil C stabilization and support climate-smart agriculture goals. Low-rate CWS application also shows potential as an alternative C-rich carrier material. However, careful consideration of application rates and material properties is crucial to avoid adverse effects on native SOC mineralization.This research was funded by Chilean ANID (Agencia Nacional de Investigación y Desarrollo), through the FONDECYT Regular project Nº 1201375 and FONDECYT initiation project Nº 11201107
Impregnating multi-walled carbon nanotubes with Cyanex 923 extractant to improve harmful chromium(VI) adsorption from aqueous media
No full textThe performance of C923-impregnated multi-walled carbon nanotubes on harmful Cr(VI) adsorption from acidic solutions was investigated. C923 (Cyanex 923) is a liquid–liquid extraction reagent of the phosphine oxide type. The adsorption was investigated at various experimental conditions: stirring speed (250–1,500 min, temperature (20°C–60°C), HCl (0.5–2 M) and metal (0.01–0.2 g/L) concentrations in the aqueous phase, ionic strength of this phase, and adsorbent dosage (0.5–1 g/L). It was found that the metal adsorption is endothermic and spontaneous, responded to the moving boundary rate law and to the Freundlich isotherm. Adsorption dosage responded to two different models, at 0.5 g/L, data were fitted to the first order kinetic equation, whereas at 1 g/L, pseudo- second-order model fitted to the experimental results. Metal loaded onto the impregnated nano-tubes can be desorbed by the use of hydrazine sulphate solutions, at the same time, chromium was recovered in the solutions in the less harmful Cr(III) oxidation state.To the CSIC (Spain) for Project 202250E01
Expanding the targeted protein degradation approach with small molecule chimeras directed to the 26S proteasome
Get PDFAll data is in a zipped folder named "all_data.zip". In this zipped folder, there is one folder named Computational Data, that contains the molecular dynamics simulations results and the input files from where they were generated.Peer reviewe
Tetrahedral DNA nanostructures, graphene and carbon nanodots-based electrochemiluminescent biosensor for BRCA1 gene mutation detection
No full textIn this study, we present a novel electrochemiluminescent DNA biosensor designed for detecting breast cancer type 1 (BRCA1) gene mutations. The biosensor integrates graphene nanosheets (Graph-NS), tetrahedral DNA nanostructures (TDNs), and carbon nanodots (CNDs) to enhance sensitivity and specificity. Graph-NS are employed to structure the transducer and serve as a platform for DNA immobilization. TDNs are engineered with a BRCA1 gene-specific capture probe located at the apex (TDN-BRCA1), facilitating efficient biorecognition. Additionally, the basal vertices of TDNs are functionalized with amino groups, enabling their attachment to the CSPE/Graph-NS surface via amino-graphene interaction. This platform effectively identifies single-base mutations in the BRCA1 gene utilizing synthesized CNDs as a coreactant and [Ru(bpy)3]2+ as the luminophore through the coreactant pathway. The developed biosensor demonstrates exceptional sensitivity and can detect a single mutation in the BRCA1 gene. Furthermore, it has been successfully validated in real samples obtained from breast cancer patients, showcasing a remarkable detection limit of 1.41 aM.The Spanish Ministry of Economy and Competitiveness has financially supported this work (PID2020-116728RB-I00, PID2023-150844OB-I00, RED2022-134120-T and TED2021-129738B–I00). Spanish Ministry of Universities supported Laura Gutiérrez-Gálvez with a FPU (FPU19/06309). European Union, through Autonomous Community of Madrid supported Daniel García-Fernández with a Programa Investigo grant in from the Plan de Recuperación, Transformación y Resiliencia– NextGenerationEU (09-PIN1-00013.4/2022-Puesto 83). The authors acknowledge the support of the EU and Horizon 2020 through Instruct Proposal PID: 21859, and the CRIOMECORR project (ESFRI-2019-01-CSIC-16) to the cryoEM CNB-CSIC facility. The authors also appreciate the service from the MiNa Laboratory at IMN, and funding from EU (FEDER, FSE), MINECO (project CSIC13-4E-1794) and CM (project S2018/NMT-4291 TEC2SPACE).Peer reviewe
A Regulatory Framework for Legal Ecosystems in the Context of Emerging Web-Based Systems and the European AI Value Chain Regulations
No full textThe European Commission is making significant efforts to regulate and harmonise the digital single market, covering areas such as data processing, data flows, interoperability, exchanges, and the roles, responsibilities, and potential liabilities of owners, designers, and service providers. This Chapter takes an internal perspective of legal governance, exploring the regulatory models and instruments crucial to constructing legal ecosystems. Initially, there were nine European common data spaces to be regulated in real-time, with an additional tenth space related to the European Open Science Cloud (EOSC). Four more spaces (including human heritage and tourism) have been recently added. This Chapter delves into theproposed instruments for regulation. The central argument is that the regulation and development of platforms within the platform-driven economy, particularly in the banking and financial sectors, go beyond traditional frameworks of hetero, co-, and self-regulation. Instead, these processes involve intra-technological and computational regulation. Therefore, regulatory approaches must incorporate a formal inside-outside and a middle-out/inside-out approach derived from intelligent information systems. A comprehensive framework will be outlined to encompass hard and soft law, policies, and ethics within the context of emerging scenarios fostered by Web 3.0, Industries 4.0 and 5.0. This Chapter will delve into the common and specific regulatory instruments and mechanisms proposed for these data spaces, thoroughly examining the technical and legal frameworks required to incept and flesh out these digital ecosystems.This research has been partially funded by the EU Project OPTIMAI. Optimizing Manufacturing Processes through Artificial Intelligence and Virtualization. Grant agreement ID: 958264 (2021-2024); and by the national project SGR-506 (AQU, Research Group of Excellence at UAB, SGR 00532 2022-24)Peer reviewe
Cambios en la microbiota intestinal y en la dieta desde la edad adulta hasta la vejez
No full textResumen del trabajo presentado a la 13ª Reunión de la Red Española de Bacterias Lácticas (RedBAL), celebrada en el Centro de Investigaciones Biológicas (CIB-CSIC) en Madrid del 17 al 18 de junio de 2019
Estrategias dietéticas para modular la microbiota intestinal: probióticos y prebióticos
No full textTrabajo presentado en el V Congreso de los Jóvenes Biotecnólogos del Norte de España, celebrado en Oviedo (España), del 15 al 17 de noviembre de 201
Monitoring of volatile fatty acids during anaerobic digestion of olive pomace by means of a hand held near infrared spectrometer
No full text3 Figuras.-- 2 TablasThe accumulation of volatile fatty acids (VFAs) over anaerobic digestion (AD) leads to malfunctioning of industrial reactors, hence decreasing biogas production. Real-time monitoring of VFAs is a challenge due to the complexity and high cost of current methods for their quantification. For this reason, this research evaluated the application of near infrared (NIR) spectroscopy to quantify volatile fatty acids as a tool for AD reactors monitoring. To do that, 129 samples from various AD reactors fed with olive oil pomace were taken and their NIR spectra were acquired with a hand-held spectrometer. After performing grid search, three spectral variable selection methods, namely competitive adaptive reweighted sampling, uninformative variable elimination (UVE) and successive projections algorithm, were assayed before developing PLRS models to correlate the NIR light transmittance through the samples at the wavelengths selected by those methods with their VFAs concentrations. UVE led to the best performance for all the VFAs assayed. Thus, R of validation of UVE-PLSR models for acetic, propionic, butyric, valeric and total VFAs were 0.895, 0.622, 0.866, 0.898 and 0.871, respectively. The predictive model for total VFAs achieved the highest accuracy (RMSEV = 539.5 mg/L), explained by the correlation between the light absorption at the wavelengths selected by UVE and the chemical characteristics of VFAs. All in all, the prediction errors achieved suggest that a portable near infrared spectrometer can be used for monitoring VFAs in AD processes.The authors would like to thank to the Spanish Ministry of Economy, Industry and Competitiveness for funding this research through the projects PID2022-137299OB-100 and PID2020-116698RB-100. The authors also acknowledge Danae Abigail Candelera Juracan (C2 Proficiency qualification) for revising and improving the language of this manuscript and her insightful suggestions. Fangchen Ding would like to thank the China Scholarship Council for the PhD scholarship No. 202306850014.Peer reviewe