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    DIT 686/24 - Elevate UltraPly TPO

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    Los sistemas de impermeabilización Elevate UltraPly TPO, formados por láminas de caucho sintético autoprotegida aptas a la intemperie, están destinados a la impermeabilización de cubiertas con y sin pendiente (en caso de renovaciones) tanto en obra nueva como en rehabilitación. Este sistema ha sido evaluado para su uso en cubiertas: accesibles para el mantenimiento de las mismas, con o sin aislamiento, lastradas- ajardinadas (extensivas, semi-intensivas e intensivas), vistas (adheridas o fijadas mecánicamente), con pendientes ≥ 1 % en obras nuevas y ≥ 0 % en obras de rehabilitación

    An outline on the chemical phenotype flexibility of forest species: an eco-metabolomics study of Pinus uncinata along an altitudinal gradient

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    The altitudinal distribution of plant populations is mainly determined by a set of environmental variables, including temperature, water availability, UV radiation, among others, which gradually shift with elevation. Therefore, altitudinal gradients in ecology could serve as “natural laboratories” providing insights into the phenotypic flexibility of natural plant populations. Plants can adjust their phenotypes to cope with specific environments. However, the adjustment capacity directly depends on the plasticity and flexibility of plant phenotypes. Plants growing at the edges of their distribution gradients may present limited flexibility due to the sub-optimal environmental conditions they experience. We analyzed the foliar metabolomes of a mountain pine population in the Pyrenees to assess their chemical phenotypic flexibility along an altitudinal gradient. We found significant changes in foliar metabolomes across different altitudes, with the most contrasting foliar metabolomes observed at the lowest and highest altitudes. Trees growing at the boundaries of the altitudinal distribution considerably shifted their foliar metabolome compared to those at more central locations with an overall upregulation of sugars, amino acids, and antioxidants. Metabolomics analyses suggested higher oxidative activity at lower altitude, presumably due to the drier and warmer conditions. However, oxidative stress indicators were also detected at the tree line, potentially associated with chilling, UV, and tropospheric O3 exposure. In addition to the inability of many species to keep pace with the rapid speed of climate change by migrating upward in altitude or latitude to find more optimum environments, their migration to higher elevations may be hindered by the presence of other environmental factors at high altitudes. Eco-metabolomics studies along environmental gradients can provide crucial insights into the chemical phenotypic flexibility of natural plant populations while providing pivotal clues regarding which plant metabolic pathway are prioritized to cope with specific environments.This research was supported by a research fellowship (JAE) from the CSIC, the Autonomous Agency of National Parks of Spain (OAPN) project 2948/2022 (MetForDec), the Spanish Government grants PID2022–142746NB-I00, PID2020–115770RB-I, PID2022–140808NB-I00, and TED2021–132627B–I00 funded by MCIN, AEI/10.13039/ 501100011033 European Union Next Generation EU/PRTR, the Fundación Ramón Areces grant CIVP20A6621, and the Catalan Government grants SGR 2021–01333 and AGAUR2023 CLIMA 00118. MO and OU were supported by the project of the Ministry of Education, Youth and Sports of the Czech Republic (AdAgriF; CZ.02.01.01/00/22_008/0004635).Peer reviewe

    IFI27-HA-bound proteins identified by mass spectrometry

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    HEK-293T cells were transfected with a pCAGGS plasmid encoding for IFI27 fused to HA tag (pCAGGS-IFI27-HA) or with a pCAGGS empty plasmid, as control. 24 hours later, cells were treated with Poly(I:C) at 3000 ng/ml. At 24h after poly(I:C) transfection, protein extracts were obtained by lysis and were incubated with HA-bound agarose beads to retain IFI27-HA and all its associated proteins, which were then identified by MS. The proteins identified only in the IFI27-HA-overexpressing cells are shown. Protein accession number (prot_acc), protein description (prot_desc), prot_score, prot_mass, prot_matches_sig, prot_sequences_sig, prot_coverage and prot_PI (isoelectric point) are indicated, being these terms previously explained in (https://www.matrixscience.com/help/csv_headers.html).Peer reviewe

    Biological process organization of mass spectrometry data

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    All proteins found on IFI27 interactome with a score higher than 40 (S1 Table) were analysed by Gene Ontology, using NIH DAVID software (https://davidbioinformatics.nih.gov/). Proteins were classified by biological process, and we selected all those groups of proteins whose function showed a False Discovery Rate (FDR) lower than 0.05. Category of classification, term of biological process, count of proteins within that biological process, percentage of the total of proteins represented by that biological process, pvalue, Fold Enrichment and False Discovery Rate (FDR) are indicated.Peer reviewe

    ETE 18/1081 - PURTOP EASY SYSTEM ROOF

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    PURTOP EASY SYSTEM ROOF es un impermeabilizante líquido mono-componente basado en poliuretano puro, constituido por resinas de poliuretano mono-componente elastomérico, no armado (sólo en puntos singulares: desagües, elementos pasantes, etc.) de aplicación «in situ»; el cual una vez polimerizado conforma un revestimiento elástico, formando una capa continua y totalmente adherida al soporte (hormigón, mortero, metal, cerámica, espuma de poliuretano (PU) y otras membranas impermeabilizantes como PVC. EPDM y bituminosas). El espesor mínimo del producto aplicado y seco debe ser de 1,2 mm

    ETE 20/0086 - Sistema GEOPET NOISEFREE

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    Esta evaluación técnica europea se aplica a los productos térmicos de fibras de poliéster elaborados en la planta de fabricación denominados como: «GEOPET NOISEFREE». Este producto se instala de acuerdo con las instrucciones de diseño e instalación del fabricante, depositadas en IETcc([1]). El fabricante es responsable en última instancia de GEOPET NOISEFREE, que consiste en mantas hechas de fibras de poliester

    ETE 20/0896 - PULMOR SATE

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    Esta ETE se aplica a los Sistemas de Aislamiento Térmico por el Exterior (SATE/ETICS) con revestimientos continuos “PULMOR SATE” para ser aplicados como aislamiento térmico por el exterior en las fachadas de los edificios. Las fachadas son de mampostería (ladrillos, bloques, piedras) o de hormigón (preparado in situ o como paneles prefabricados) con o sin sistemas de enfoscados. Dependiendo de los métodos de fijación, el sistema se define como: SATE adherido con fijaciones mecánicas suplementarias con panel EPS y XPS y, SATE fijado mecánicamente con adhesivo suplementario en panel MW, El número mínimo de fijaciones es de 6 para EPS, XPS y MW y 8 en edificios con una altura superior a 24 m

    ETE 21/0365 - WT1 ST, WT1 SH, WT1, WT1 SST

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    El anclaje WT1 ST en el rango M8, M10, M12, M16, M20 y M24 es un anclaje fabricado en acero cincado. El anclaje WT1 SH en el rango M8, M10, M12, M16 y M20 es un anclaje fabricado en acero sheradizado. El anclaje WT1 en el rango M8, M10, M12, M16 y M20 es un anclaje fabricado en acero cincado. El anclaje WT1 SST en el rango M8, M10, M12, M16 y M20 es un anclaje fabricado en acero inoxidable. El anclaje se instala en un agujero previo cilíndrico y se fija mediante expansión por par controlado. La fijación está caracterizada por fricción entre la grapa de expansión y el hormigón.Peer reviewe

    Microbiological hazards associated with the use of water in the post-harvest handling and processing operations of fresh and frozen fruits, vegetables and herbs (ffFVHs)

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    504 pages.-- External Scientific Report.-- Open accessThis tender, developed under a self-task mandate from the BIOHAZ Panel, analysed the characteristics of the water and the practices followed by the European food business operators (FBOs) to maintain process water quality used during the post-harvest handling and processing operations for fresh and frozen fruits, vegetables and herbs (ffFVHs) using: information and data obtained from FBOs, experimental data extracted from literature and dynamic mass balance modelling. Quantitative data were obtained from 61 FBO scenarios (29 from the fresh-whole sector, 19 from the fresh-cut sector, and 13 from the frozen sector). The impact of no water treatment was evaluated in 17 scenarios, while in 44, the challenges of maintaining the microbiological quality with water disinfection agents were examined, including chlorine, peroxyacetic acid, and hydrogen peroxide. The findings highlighted that when no water disinfection treatment was used Listeria monocytogenes was detected in some scenarios of the fresh-whole and frozen FVH sectors as well as Salmonella, pathogenic Escherichia coli and norovirus in the fresh-cut and frozen FVH sector. Additionally, inadequate or improper monitoring systems resulted in either excessively high or insufficient disinfectant concentrations in the water, which, when too low, failed to sufficiently reduce the microbial load. The literature review revealed a tendency to: focus on leafy greens, use chlorine-based disinfectants, and employ chemical oxygen demand (COD) as the primary physico-chemical parameter, with total dissolved solids (TDS) and turbidity considered to a lesser extent, as indicators of water quality. Additionally, dynamic mass balance modelling was used to interpret experimental data from literature and FBOs. The model was fundamental to estimate key unknown parameters, predict the microbial contamination and accumulation of organic matter and allowing to simulate “what-if scenarios.”Peer reviewe

    Circulating progesterone concentrations and pregnancy outcomes in high-producing lactating dairy cows treated with human chorionic gonadotropin on day 2 of the estrus cycle

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    Suboptimal concentrations of circulating progesterone (P4) in the early postovulatory period have been associated with low fertility observed in high-producing lactating dairy cows. The administration of human chorionic gonadotropin (hCG) increases P4 in cattle by stimulating its endogenous production by the corpus luteum (CL) and creating an accessory CL if administered at an appropriate stage of the cycle. The aim of this study was to evaluate the effect of a single administration of hCG on d 2 of the estrus cycle on circulating P4 concentrations and pregnancy per artificial insemination (P/AI) in high-producing lactating dairy cows in confinement systems. To that end, 796 lactating Holstein-Friesian dairy cows from 15 farms were enrolled on this study. The mean ± SD parity and DIM at enrollment were 2.3 ± 1.4 and 86.7 ± 17.8, respectively. After a voluntary waiting period (50-60 d after calving), cows underwent fixed-time artificial insemination (FTAI) following a routine protocol for synchronization of ovulation (Double Ovsynch, G6G, or 7-d Ovsynch + P4). Cows on each farm were blocked on parity, DIM, BCS, and synchronization protocol and were randomly assigned to receive either 3,000 IU of hCG (n = 420) or an equivalent volume of saline solution (control; n = 376) on d 2 after estimated estrus (∼16 h before FTAI = d 0). Blood samples were collected from a subset of cows from both treatments (control n = 65 and hCG n = 65) on d 0, 7, and 14 of the estrus cycle to measure serum P4 concentrations. Pregnancy per artificial insemination on d 30 after FTAI was affected by hCG treatment and parity. Moreover, there was a tendency for an interaction between treatment and parity. Overall, treatment with hCG on d 2 of the estrus cycle increased P/AI (45.2% vs. control 38.8%). In first- or second-lactation cows, P/AI was similar between hCG-treated and control cows (47.1% vs. control 45.9%). Conversely, cows in third or greater lactation treated with hCG had greater P/AI (42.1% vs. control 27.3%). The overall incidence of pregnancy loss between d 30 and d 70 was 14.7% and was not affected by treatment. Cows treated with hCG had higher P4 concentrations on d 7 and 14 compared with control cows (3.4 ± 0.66 vs. 3.0 ± 0.58 ng/mL and 6.6 ± 1.28 vs. 5.3 ± 1.02 ng/mL, respectively). Moreover, an interaction between treatment and parity revealed that P4 concentrations were higher in hCG-treated cows in third or greater lactation compared with control cows in the same parity group on d 7 and 14, while no differences were observed in first- or second-lactation cows. In conclusion, administration of hCG on d 2 of the estrus cycle increased fertility in high-producing dairy cows in third or greater lactation, in association with an early increase of circulating P4.This work was funded by research projects RYC2021-033574-I and TED2021-129764B-I00 funded by MCIN/AEI/10.13039/501100011033 (Spain) and by European Union Next GenerationEU/PRT.Peer reviewe

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