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ANALISA KADAR PROTEIN CRUDE ENZIM SELULASE DARI KAPANG Rhizopuz Sp PADA SUBSTRAT AMPAS TEBU HASIL ISOLASI DARI KEBUN CENGKEH, KARE, MADIUN
Get PDFKapang Rhizopus sp merupakan salah satu mikroorganisme yang memiliki kemampuan tinggi untuk menghasilkan enzim selulase.Enzim selulase merupakan enzim yang dapat menghidrolisis selulosa. Hidrolisis meliputi proses pemecahan polisakarida di dalam biomassa lignoselulosa, yaitu: selulosa dan hemiselulosa menjadi monomer gula penyususnnya. Penelitian ini bertujuan untuk mengetahui produksi dan aktivitas enzim selulase terhadap aktivitas crude enzim selulase dari kapang Rhizopus sp dengan subsrtat ampas tebu (bagase). Metode penelitian menggunakan kuantitatif eksperimen dengan pola rancangan acak lengkap (RAL) dua faktorial. Perlakuan penelitian meliputi perbedan inokulum (K) yaitu 5% (K1), 15% (K2), 25% (K3) dan lama fermentasi (T) yaitu 3hari (T1), 6hari (T2), 9hari (T3), dan 12hari (T4). Data yang diambil dari perlakuan tersebut adalah kadar protein dengan metode brownstead lowry. Analisis data menggunakan variansi anava dua jalur dengan taraf signifikansi 5% setelah itu dilanjutkan dengan uji Beda Nyata Terkecil (BNT ). Hasil penelitian menunjukkan bahwa: Fhit > Ftab sehingga ada pengaruh antara konsentrasi inokulum dan lama fermentasi terhadap aktivitas crude enzim selulase dari kapang Rhizopus sp, Perlakuan perbedaan konsentrasi dan lama fermentasi mendapatkan kadar protein tertinggi 0,715 dengan konsentrasi 25% dan lama fementasi 25
SKRINING AKTIVITAS ANTIBAKTERI DAUN KELOR (Moringa oleifera), DAUN BIDARA LAUT (Strychnos ligustrina Blume), DAN AMOXICILIN TERHADAP BAKTERI PATOGEN Staphylococcus aureus
Get PDFExploration of a variety of plants as a source of antibacterial has been done, but it is not all plants effective to inhibit the growth of bacteria. This study aims to determine the difference between the ability of the antibacterial activity of plant leaves Moringa oleifera and Strychnos ligustrina Blume against Staphylococcus aureus bacteria growth. This research is an experiment with a completely randomized factorial design (CRD) 3x4 with four replicates. The materials use is ethanol extract of the leaves of Moringa leaf extract, S. ligustrina, and amoxicillin. Testing the antibacterial activity performed on Mueller-Hinton agar media with the diffusion method. The analysis and interpretation of results were done by ANOVA 95% (α: 0.05). Susceptibility bacteria to antibiotics, and plant extracts assessed according to standard NCCLS (National Committee for Clinical Laboratory Standards). The antibacterial agent (ethanol extract of Moringa leaf, ethanol extract of S. ligustrina leaf, and amoxicillin) and concentrations influence significantly inhibit the growth of S. aureus bacteria. S. aureus bacteria are sensitive only at 100% concentration with inhibition zone diameter 21.5 ± 5:35 mm, when given the leaf extract of S. ligustrina, and at a concentration of 75% with a diameter of 14.3 ± 1.32 mm is intermediate. The bacteria behave-resistant to the compound of M. oleifera leaf extract and amoxicillin at all concentrations. Diameter of zones of inhibition amoxicillin equal 9.99 ± 2.02 mm with 100% concentration treatment. While the treatment of M. oleifera leaf extract, at a concentration of 75% and 100%, resulting in the largest inhibition zone each is 3.9 ± 0.64 and 3.82±0.57 mm
PEMANFAATAN KULIT BUAH JERUK NIPIS (Citrus aurantifolia) SEBAGAI LARVASIDA Aedes aegypti INSTAR III
Get PDFDHF (Dengue) disease is transmitted through mosquito vectorAedes aegypti.One way to do control the Dengue vector is to use insecticide made from natural materials and is environmentally friendly with lemon peel extract (Citrusaurantifolia). This study aims to determine the effectiveness of extracts of lemon peel (Citrus aurantifolia) in killing the third instar larvae Aedesaegypti with various concentrations and time variations. This were an experimental laboratory researchs using various concentrations of extract limes peel (Citrusaurantifolia) 1%, 2%, 3%, 4%, 5%, 6%, and 7% in which there 25 larvae Aedes aegypti and using a variation of 30, 60, 90, 120, 180, 240, 300, and 1440 minutes after contact with the extracts limes peel (Citrusaurantifolia). Effective concentration to kill larvae of Aedes aegypti by 50% (IC50) was the concentration of 3.419%
SKRINING BAKTERI SELULOLITIK ASAL TANAH KEBUN PISANG
Get PDFCellulose is the most abundant biomass in nature. Utilization of organic wastes can be improved by changing the cellulose from wastes into products which have the economic value with the help of cellulolytic bacteria. Cellulolytic bacteria are bacteria that have the ability to produce cellulase. Cellulase can degrade cellulose as the substrate. The aim of this study were to obtain cellulolytic bacteria, and to provide an information about the test of cellulolytic bacterial activity. Stages of this study are as follows: isolation and purification, morphological and physiological characterization of cellulolytic bacteria, tests enzyme activity. Results showed that there were two types of cellulolitic bacteria which had been obtained, namely isolate A1 and A2 that were able to grow on CMC media and each isolate has the ability to form a clear zone of 1.3 and 1.25 mm, respectively. Isolate A1 belonged to genus Pseudomonas with enzyme activity of 1.248 unit/ml
EFEKTIFITAS LARUTAN TEMULAWAK (Curcuma xanthorrhiza Roxb.) TERHADAP PENINGKATAN JUMLAH LEUKOSIT AYAM BROILER (Gallus gallus Domestica sp.)
Get PDFTemulawak (Curcuma xanthoriza Roxb.) is one of zingibereceae family medicinal plants that grow in Indonesia. Temulawak is a medicinal plant which also has a function as an immunomodulator. The present experiment was conducted in order to study the effect of temulawak extracts on the total of leucocytes in broiler chickens. The research was conducted in the Laboratory of Biology UIN Raden Fatah Palembang. The research was done by looking at the amount of the average increase before, 8 hours, and 22 days were treated with 3 replications. Twenty seven broiler chickens, 2 weeks old were used in this experiment, and were devided into 9 groups treatments, there were: (X0) negative control without the temulawak extract, (A1) 0,5 ml temulawak extract, (A2) 1 ml temulawak extract, (A3) 1,5 ml temulawak extract, (A4) 2 ml temulawak extract, (B1) 0,5 ml chemical antibiotics, (B2) 1 ml chemical antibiotics, (B3) 1,5 ml chemical antibiotics, (B4) chemical antibiotics. The observations showed an increase in the total of leucocytes after 8 hours of the treated solution A3 ginger in the treatment dose of 1,5 ml temulawak extract of 30016,5 μl and after 22 days treatment were given a solution of ginger at dose 1,5 temulawak extract of 29250 μl. In conclusion, temulawak extract has an activity as an immunostimulant for non specific immune respons
PENGARUH KUANTITAS GARAM TERHADAP KUALITAS BEKASAM
Get PDFFish is one of the animal protein sources consumed by people because it is relatively easy to obtain and affordable. The abundant of the fish needs some ways to preserve it, one of them is by making bekasam. Bekasam is fish fermented product which is procesed with the addition of rice and salt. One of the function of the salt is to select microorganisms that produce proteolytic enzymes.The purpose ofthis study was to know influance of different salt concentrations to ward the quality of fish bekasam.The research was conducted in August 2015 in Chemistry Laboratory of Biology Education Study Program, Teaching Training and Tarbiyah Faculty UIN Raden Fatah Palembang. The research method was an experimental laboratoryusing a CRD with 4 treatments and 3 replication. The treatments were salt concentrationK0 (10% b/v), K1 (20% b/v), K2 (30% b/v) and K3 (40% b/v). The test parameters observed were theacidity and hedonic test (color, smell and taste). The parametric data for the acidity was analyzed by ANOVA test, while nonparametric data(hedonic) used the organoleptic test. The results showed that based on ANOVA, the different salt concentration gave a very significant different influance (P=0,01) toward the acidity value, that Fcount> Ftable is 26,16 > 7,59. Based on research result, the acidity of each K0, K1, K2and K3 was 1,13 N, 1,00 N, 1,02 Nand 0,42N.Treatment of 10% salt concentration resulted the highest acidity 1,13 N.While from hedonic test showed that bekasam with 20%salt concentration was the most preferre
ANALISIS KLORIN PADA BERAS DI PASAR INDUK JAKABARING DAN SUMBANGSIHNYA TERHADAP MATA PELAJARAN BIOLOGI PADA MATERI MAKANAN BERGIZI DAN MENU SEIMBANG DI KELAS XI SMA/MA
Get PDFRice is a staple food that easily processed easy to prepare, delicious and contains carbohydrate and protein as energy sources. Chlorine is not permitted to be used for rice because it can harm the respiratory system and the liquid can damage humans skin. The Health Ministry of Indonesian Republic already ruled about Ingredient Added Food (IAF) in rule No.722/Menkes/Per/IX/88, it said that Chlorine forbidden to used for rice because Chlorine is not the list of IAF as whitening and cooking wheat group. The purpose of this research is identify and count how much Chlorine in branded rice and inbranded rice that sold in Center Market of Jakabaring Palembang. There are 14 samples of rice in consist of 7 branded rice samples and 7 inbranded rice samples that took from 4 shops and 3 rice sell distributor in Center Market of Jakabaring Palembang. This research was done on 18- August 2015 at Chemical Laboratory of UIN Raden Fatah Palembang. Chlorine was tested by using Colour Reaction Method, Iodometry Titration Method and Organoleptic Test to identify rice physic that contain Chlorine. The result of this research showed that all samples are negative Chlorine. Based on Organoleptic Test, the sampel without Chlorine has characteristic in pure white colour, no medicine smell, not slippery and unfragile, meanwhile from the positive control that analized contain Chlorine 3,99 mg, showed that rice with Chlorine has characteristic more shine untransparant white colour, has medicine smell, slippery and fragile. In this regard it can be concluded that all sample are not contained Chlorine and save to consumed. This research after to be appropriate to the syllabus concept of K13 it can be as gift as education sector of Biology Education Subject in form Studying Execution Planning (RPP), Student Work Sheet (LKS) were have done validated by 2 validators and the result is valid, and in form Student Studying Video, too
UJI KANDUNGAN FORMALIN PADA BUAH PEPAYA (Carica papaya L.) DAN BUAH NANAS (Ananas comosus L.) YANG DI JUAL DILINGKUNGAN UIN RADEN FATAH PALEMBANG DENGAN METODE SPEKTROFOTOMETRI
Get PDFFruit is a natural product that has benefits such as giving nutrient for society, incoming source, pervading labor if it is afforded intensively. The purpose of this research is to measure whether there is formalin or not in papaya and pineapple which sold at UIN Raden Fatah environtment. The kind of this research uses qualitative descriptive appearance organolaptic test and colour test and experiment method appearance quantitative test by using spectrophotometer. The result of measuring organolaptic with color of parameter, taste, and tekstur surely, there is difference to fruit sample of different fruit seller. Colour test is done by using Schiff reagen. The result of Colour test shows all sample don’t contain formalin, noted with yellow liquid. Quantitative test uses spectrophotometer. The result of quantitative test shows all sample examinee identified formalin. Formalin level of each sample is different. The smallest formalin level is 0,0007 ppm at papaya in A seller and the highest is 0,0025 ppm at pineapple in A seller
UJI KANDUNGAN SIKLAMAT PADA LEGEN JAMU GENDONG DI KELURAHAN SEKIP JAYA PALEMBANG
Get PDFLegen is neutralizing bitter taste of herbs commonly used by herbalist. The sweet taste at the legen suspected of using artificial sweeteners. Cyclamate is one of the most common artificial sweetener used in food or beverages. In the world of education cyclamate included in class VIII SMP biology syllabus. The purpose of this research is in the legen contained cyclamate or not and the application of the experimental method (practicum) legen testing on student learning outcomes. Cyclamate content test study conducted in June 2015 and the application of the experimental method in August 2015. The method used in the testing of cyclamate is a deposition method while in the learning process used an experimental method (practicum). The results show that all samples tested legen containing cyclamate and exceeding the threshold is an average of the sample 1 (6 g / L), sample 2 (5.33 g / L), sample 3 (5.46 g / L), samples 4 (8.93 g / L) and sample 5 (6.26 g / L) while research using experimental method (practicum) menunjukka F count> F table (3.73> 2.66). The conclusion from this research that all traders use cyclamate exceeds the specified threshold and use of the experimental method affects the learning process on learning outcomes
PENGARUH PEMBERIAN KEONG SAWAH DAN AIR CUCIAN BERAS TERHADAP PERTUMBUHAN BELUT (Monopterus albus Zuieuw)
Get PDFSnail and rice water has a very high protein content so the snail meat and rice water can be used as an additional food that is good for eel. Giving snail and rice water as a feed supplement in the eel (Monopterus albus) is expected to boost growth in the eel. This study aimed to determine the effect of snails and rice water on the growth of eel (Monopterus albus) and know Where feed to get optimal results on the growth of eel (Monopterus albus). Research conducted at the Laboratory of MIPA IAIN Raden Fatah Palembang. This study used a Rancangan Acak Lengkap (RAL) with 4 treatments and 6 replications. Such treatment is A : pellets 4.5 grams, B : 150 ml of rice water + 3 grams of snail, C : 100 ml rice water + 3.5 grams of snail, D : 50 ml rice water + 4 grams of snail. The analysis showed that the highest growth in treatment D is 50 ml rice water + 4 grams of snail. The conclusion is giving snail and rice water in the eel (Monopterus albus) significant effect on the growth of the body\u27s weight and length of the eel