Jurnal Biota
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Perilaku Merokok di Dalam Rumah dan Kejadian Penyakit Tuberculosis Paru Sebagai Sumber Pembelajaran
Get PDFThis research is motivated by smoking behavior in the house with the incidence of pulmonary tuberculosis. The formulation of the problem in this research is How the smoking behavior in the house and the incidence of pulmonary tuberculosis disease as a source of a learning. The purpose of this research was to determine of smoking behavior in the house with the incidence of the pulmonary tuberculosis disease as a source of a learning. Research method is used descriptive qualitative. Population and sample in this research is community in outbreak region 3-4 Ulu Palembang which is patient with tuberculosis lung disease based on data obtained from puskesmas 4 Ulu Palembang on between 2016 and 2017 and from doctor practice in region of 3-4 Ulu Palembang. Data collection techniques in this study using a questionnaire, while data analysis techniques using statistics descriptive. Based on the results of research and discussion of research, description of smoking behavior in the house and the incidence of pulmonary tuberculosis is true and done by respondents in Village 3-4 Ulu Palembang which is a patient of pulmonary tuberculosis disease obtained from the primary data in this study and it can be concluded that the behavior of smoking in the house can cause epidemic pulmonary tuberculosis disease although there are also respondents who are not active smokers but they are exposed to pulmonary tuberculosis disease which is probably caused by anything else obtained from the secondary data in this study
ANALISIS FENOTIPE DAN PLOIDI TANAMAN MELON (Cucumis melo L.) HASIL PERLAKUAN EKSTRAK ETANOLIK DAUN TAPAK DARA (Catharanthus roseus [L] G. Don.)
Get PDFAnti-mitotic agents such as colchicine have been used to induce polyploidy in various plants. On the other hand, vincristine and vinblastine are also antimitotic agent extracted from Periwincle (Catharanthus roseus [L] G. Don) were previously studied to produce autotetraploid on shallot tuber (Allium cepa L.). Therefore, in this study phenotype character and ploidy of muskmelon (Cucumis melo L.) produced by etanolic extract of periwikle leaves were determined. The effects of different concentration of etanolic extract of periwinkle leaves on polyploidy induction in muskmelon were examined. Melon seedling of two days old were immersed in 0.5%, 0.1%, and 0.05% for 8 hours. Then seedling was grown on the polybag and a drop of each concentration of periwinkle leaves’s etanolic extract was added into apical shoot. Melon seedling of ten days old were moved and cultivated and harvested on 60 days after cultivation. Phenotypic character such as: plant high, stem diameter, leaf area, fruit weight, fruit area around, flesh fruit thickness, skin fruit thickness, fruit horizontal diameter, fruit, vertical diameter, number of seeds, weight of 100 gram of seed, seed leght, seed width, and seed thickness were examined. The ploidy degree was determined by count of chromosome number root tips of second generation muskmelon sprout. Result of this study revealed that 0.05% etanolic extract of periwinkle leaves for 8 hours immersed is optimum concentration to induce autotetraploid muskmelon (4n=48). Autotetraploid phenotypic character of muskmelon produced by 0.05% etanolic extract of periwinkle leaves were generally bigger than control plants statistically significant in stem diameter, leaf area, and fruit horizontal diameter. The result also showed that the chromosome number of second generation autotetraploid muskmelon sprout was tetraploid (4n=48)
ANALYSIS OF FLOOD DISTRIBUTION IN SUB-WATERSHED BENDUNG BY USING GEOGRAPHIC INFORMATION SYSTEM (GIS)
Get PDFThe research was conducted to map the level of flood vulnerability in the area around Sub DAS Bendung and Analyzing the influence of natural factors causing flood and land cover factors on flood vulnerability in the area around Sub DAS Bendung. In the rainy there are floods in some locations. The Flood of Sub DAS Bendung are influenced by several factors including natural factors such as river characteristics, which have small river channel capacity, some areas that are swamp, sea tides that resist the flow of river, and relatively flat topography conditions, so some Sub DAS Bendung area is a flood plain. Studies of the main causes of flooding in Palembang City or in other areas are very important. The use of GIS as an analytical tool will make it easier to examine the factors causing flooding to classify areas with high flood vulnerability. Thus expected to profide solutions and reduce the impacts caused by flood
EFEKTIVITAS BERBAGAI KONSENTRASI EKSTRAK DAUN KAYU MANIS (Cinnamomum burmannii [Ness.] BI) TERHADAP DIAMETER ZONA HAMBAT PERTUMBUHAN BAKTERI Staphylococcus epidermidis
Get PDFStaphylococcus epidermidis is one of the bacteria that can cause infectious diseases. In general Staphylococcus epidermidis can cause diseases of swelling (abscess) such as skin infections or acne. Cinnamon (Cinnamomum burmannii [Ness.] BI) leaf extract has a substance which gives antimicrobial effects that can be used as an inhibitor of the Staphylococcus epidermidis bacteria’s growth because it contains substances such as essential oils, flavonoids, saponins, tannins, and alkaloids. The aim of this research is to analyse the effect of the concentration of cinnamon leaf extract and to determine the concentration of extract which has the best influence to the inhibition zone diameter of Staphylococcus epidermidis bacteria. This research was conducted in Biology Laboratory of University of Muhammadiyah Malang, on 17-18 May 2017. The results showed that there was an effect of giving various concentrations of cinnamon leaf extract to the inhibition zone diameter of Staphylococcus epidermidis bacteria’s growth. The concentration of cinnamon leaf extract which has the best influence to the inhibitory zone diameter of Staphylococcus epidermidis bacteria is 100% with the mean of inhibitory zone diameter 15.16 mm
ISOLASI DAN IDENTIFIKASI BAKTERI TERMOFILIK PEREDUKSI SULFAT DI AIR PANAS SARONGSONG KOTA TOMOHON
Get PDFABSTRAK.Bakteri Pereduksi Sulfat (BPS) berperan dalam biodegradasi lahan tercemar. Identifikasi berdasarkan karakter fenotipik perlu dilakukan untuk mengetahui jenis bakteri ini. Penelitian ini bertujuan untuk mendapatkan beberapa isolat bakteri pereduksi sulfat di lokasi pemandian air panas Saronsong kota Tomohon. Identifikasi dilakukan dengan menggunakan metode profile matching. Bakteri termofilik pereduksi sulfat diisolasi dari lokasi pemandian air panas Sarongsong kota Tomohon menggunakan media cair postgate b yang telah dimodifikasi dan selanjutnya dilakukan pemurnian menggunakan metode pengenceran. Karakter fenotipik isolat bakteri termofilik pereduksi sulfat yang diuji meliputi morfologi koloni, morfologi sel dan karakter biokimia. Hasil isolasi pada kawasan air panas Sarongsong diperoleh 4 isolat bakteri termofilik pereduksi sulfat yang dilihat dari perubahan warna media selektif. Berdasarkan identifikasi dengan menggunakan metode profile matching menunjukkan bahwa keempat isolat tersebut diduga termasuk dalam anggota genus Desulfotomaculum (isolat AK4D3 dan L4D1), genus Desulfomicrobium (isolat AJ4D5) dan genus Desulfobulbus (isolat L5D2).Kata Kunci:, fenotipik, identifikasi, isolasi, karakterisasi ABSTRACT. Sulfate Reducing bacteria play a role in biodegradation of land contaminated. Identification based on phenotype character needs to be done to know this type of bacteria. The objective of this research is to get some isolate of sulfate reducing bacteria in clear water, cloudy water and mud in Tomohon hot spring water location. Identification is done by using profile matching method. The sulfate reducing thermophilic bacteria was isolated from the Tomohon Sarongsong hot spring location using the modified postgate b liquid medium and then purified by dilution method. Phenotypic characteristics of sulfate reducing thermophilic bacteria isolates tested included colony morphology, cell morphology and biochemical character. The result of isolation at Sarongsong hot bath obtained 4 isolates of sulfate reducing thermophilic bacteria seen from selective media color change. Based on the identification using the profile matching method showed that the four isolates were suspected to be included in the members of the genus Desulfotomaculum (isolates of AK4D3 and L4D1), genus Desulfomicrobium (isolate AJ4D5) and genus Desulfobulbus (isolate L5D2). Keywords:, characteristics, Identification, Isolation, Phenotypi
KEANEKARAGAMAN ZOOPLANKTON DI RAWA BANJIRAN DESA SEDANG KECAMATAN SUAK TAPEH KABUPATEN BANYUASIN
Get PDFThe purpose of this research is to determine the diversity of zooplankton in the swamp flood waters in Sedang Village, Subdistrict Suak Tapeh, District Banyuasin. This research was conducted in Juli in the swamp flood waters in Sedang Village, Subdistrict Suak Tapeh, District Banyuasin. Determination of sampling stationsbased on purposive random sampling. Sampling of fitoplankton was done in 3 stations, they are: Swamp Nebong Kuning (station 1), Swamp Poron (station 2) and Swamp Banan (station 3). Based on the results of the research was found that the type of zooplankton in swamp flood waters in Sedang Village, Subdistrict Suak Tapeh, District Banyuasin consist of 3 classes, they are Monogonota, MastigophoradanCiliata. Diversity index of zooplankton species in the swamp flood waters in Sedang Village, Subdistrict Suak Tapeh, District Banyuasin in Swamp Nebong Kuning was medium, whereas in the Swamp of Poron and the Swamp of Banan was low
ANALISIS KERAPATAN EKOSISTEM MANGROVE DI PULAU PANIKIANG DAN DESA TONGKE-TONGKE SULAWESI SELATAN
Get PDFThe mangrove ecosystem has different density levels per location. It can be influenced by environmental factors or because of human assistance. This research was conducted in Tongke-tongke Village, Sinjai Regency and Pannikiang Island, Barru District. Observations were conducted on three groups, namely groups of trees, stakes, and seedlings. Tree group used plot size 10 m x 10 m, stakes group used plot size 5m x 5m, and the group of seedlings used plot size 1 m x 1m. The result of tree group density will be compared with the standard criteria of mangrove damage of the Minister of Environment to know the criteria and the level of density. The mangrove ecosystem in Tongke-tongke village is overgrown by Rhizophora mucronata and Avicennia sp. and is dominated by R. mucronata with very dense density. In the tree group, R. mucronata has a density of 8020 Ind Ha-1. The mangrove ecosystem in Pannikiang Island is overgrown with Rhizophora apiculata, Rhizophora stylosa, Sonneratia alba, Aegiceras floridum, Ceriops tagal, Avicennia sp., Excoearia agallocha, and Lumnitzera racemosa and has a rare and moderate density. In the tree group, the highest density in C. tagal with a density of 1270 Ind Ha-1 and indicated moderate density. Competition is always there in the ecosystem. The species of this ecosystem is fighting for space and nutrients. Therefore, competition can determine the density of plants in it
KONSENTRASI HAMBAT MINIMUM FRAKSI BIOAKTIF RIMPANG TEMULAWAK TERHADAP JAMUR Candida albicans
Get PDFCandida albicans is a microbiota in the human body that is opportunistic. The infection caused by C.albicans is called candidiasis. People in some areas in Indonesia take advantage of rhizome temulawak for the traditional treatment of various diseases such as whiteness. The objective of the study was to determine the Minimum Barrier Concentration of active fraction of methanol extract of temulawak rhizome on Candida albicans. The ginger rhizomes were extracted in stages using soxhlet, then fractionation was done using Liquid Chromatography (KCV) method after the active fraction was then determined the minimum inhibitory concentration of the active fraction against C.albicans. The result showed that the minimum inhibitory concentration of the active fraction of temulawak rhizome was 62,5 μg / ml to C.albicans. The KHM value of 125 μg/ml is included in a very strong antifungal activity meaning that the temulawak rhizome has good potential to be a phytopharmaca.Jamur Candida albicans merupakan mikrobiota pada tubuh manusia yang bersifat oportunistik. Infeksi yang disebabkan oleh C.albicans disebut kandidiasis. Masyarakat di beberapa daerah di Indonesia memanfaatkan rimpang temulawak untuk pengobatan tradisional berbagai penyakit diantaranya untuk keputihan. Tujuan penelitian untuk menentukan Konsentrasi Hambat Minimum dari fraksi aktif ekstrak metanol rimpang temulawak terhadap Candida albicans. Rimpang temulawak diekstraksi secara bertingkat menggunakan soxlet, selanjutnya dilakukan fraksinasi menggunakan metode Kromatografi Cair Vakum (KCV), setelah didafatkan fraksi aktif maka dilakukan penentuan konsentrasi hambat minimum dari fraksi aktif tersebut terhadap terhadap C.albicans. hasil penelitian didapatkan bahwa konsentrasi hambat minimum dari fraksi aktif rimpang temulawak adalah 125 µg/ml terhadap C.albicans. Nilai KHM 125 μg/ml termasuk ke dalam aktivitas anti jamur yang kuat artinya rimpang temulawak memiliki potensi yang baik untuk dijadikan bahan fitofarmaka
PENGARUH AIR LIMBAH INDUSTRI SUSU TERHADAP PERTUMBUHAN DAN HASIL TANAM TANAMAN BAWANG MERAH (Allium cepa L.)
Get PDFDuring this time the handling of waste water in dairy industry (ALIS) in Indonesia is done by dumping into the river. This activity causes damage to rice fields which are located around the river flow. The yield losses due to ALIS is up to 50%. The friendly method is needed for handling ALIS. Since ALIS is known to contain beneficial macro nutrients for plants growing so potents as an organic fertilizer. This study aimed to optimize the ALIS concentrations (0, 20, 40, 60, 80 and 100% v / v) for the growth and yield of onion (Allium cepa L.) var. Buto Ijo. The results showed, treatment ALIS 60% (v / v) increasedheight ofonion leaves until 24.45 cm. Whereas ALIS treatment on various concentrations had no effect on the number of onion shoots. The yields showed, the highest of fresh weight of biomass, number of bulbs, fresh weight and dry weight of bulbs per clump approximatly 13.03 g g; 12,33 bulbs, 7.79 g and 5.38 g was achieved by treatment of ALIS 80% (v/v). Based on the result ofgrowth and crop yields, ALIS 80% (v/v) was selected as the optimum concentration for onion crop. The ALIS was given100 mL and three times during the growing season
PENGARUH EKSTRAK BUAH PISANG dan EKSTRAK BUAH ALPUKAT TERHADAP KADAR KOLESTEROL MENCIT BETINA
Get PDFPenelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak daging buah alpukat (Persea americana Mill) dan ekstrak daging buah pisang cavendish (Musa cavendishii) terhadap kadar kolesterol total mencit. Penelitian ini menggunakan 24 ekor mencit betina yang memiliki berat badan antara 35-45 g. Hewan coba dikelompokkan menjadi empat kelompok sebagai berikut, P1:Kontrol diberi aquades; P2: diberi ekstrak buah pisang; P3: perlakuan yang diberi ekstrak buah alpukat; P4; perlakuan yang diberi ekstrak buah pisang dan ekstrak buah alpukat. Ekstrak diberikan selama 5 hari secara per oral sebanyak 2 ml dengan dosis masing-masing ekstrak 6,6 g. Darah diambil dari jantung mencit kemudian diukur kadar kolesterol menggunakan alat cholesterol check . Hasil menunjukkan bahwa tidak terjadi peningkatan yang berarti pada kadar kolesterol total mencit. Kadar kolesterol mencit yaitu P1: 148,33mg/dL; P2: 148,83mg/dL; P3: 148,83mg/dL; P4: 148,83mg/dL. Hal tersebut mengindikasi bahwa tidak ada pengaruh pemberian ekstrak daging buah alpukat (Persea americana Mill) dan ekstrak daging buah pisang cavendish (Musa cavendishii) terhadap kadar kolesterol mencit