Jurnal Ilmu Ternak dan Veteriner
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Effectiveness of bioactive combinations of several plant substances to inhibit the growth of Escherichia coli and Salmonella sp.
Get PDFThe use of antibiotic growth promoters (AGP) has been banned as feed additives in many countries, therefore the alternatives need to be found. An in vitro experiment was conducted to study the potential of combination of some plant extract to inhibit growth of pathogen bateria that normally occur in the poultry gastro intestinal tract.The combination of three plants bioactives (liquid smoke of cashew shells of Anacardium occidentaleor CLS, Phyllanthus niruri L. extract (EM), and Synzygium aromaticum extract (EDC) were formulated and evaluated for its effectiveness to inhibit growth of Escherichia coli and Salmonella sp. in vitro.The mixtures (KE) were then made in 3 different concentrations, i.e. 100 % KE, 50% KE, and 25% KE and studied their effectiveness to inhibit growth of E. coli or Salmonella sp. using microplate reader method. The results showed that the higher the concentration of the bioactive combination (KE100) the higher the ability to inhibit the growth of E. coli or Salmonella sp. The combination of bioactive substances CAM, EM, and EDC more effectively than Zn-bacitracin antibiotics to inhibit the growth of E. coli and Salmonella sp. The optimum concentration of KE with the similar effectiveness as the AGP was 25%. It was concluded that the combination of CAM, EM, and EDC was able to inhibit the growth of E. coli and even capable to eliminate the presence of Salmonella sp. In the biological assay, a combination of CAM, EM, and EDC either extract or powder form, high dose, medium or low does not affect the weight of the spleen, bursa fabricius, and blood profile. The best combination of CAM, EM, and EDC extracts to decreases the total bacterial population and E. coli was middle dose ie 0.0625% (extract) and 0.625% (powder). Likewise for live weight gain that was a good dose of extract or powder form can replace antibiotics. It was concluded that combinations of CAM, EM, and EDC had potential as a substitute for AGP in poultry feed, especially chickens
Growth and feed utilization of Boer x Kacang crossbred goats offered total mixed rations of different protein and energy levels
Get PDFThe aim of this study was to evaluate the growth of and feed utilization by Boer x Kacang crosses goats fed on total mixed ration differing in protein and energy levels. Four total mixed rations combination were formulated to contain 16 and 18% crude protein and 2650 and 2850 Kcal ME/ kg DM (dried matter) energy density. Twenty-eight male Boer x Kacang crosses goats (14.5 ± 1.14 kg) and of age ranging from 4 to 5 months were randomly allocated to one of these four TMRs (total mixed rations) (7 animals/TMR). The effects of dietary treatment were assessed using the general linear model and significance of the diet effects was detected using Duncan’s multiple range test. Dry matter intake increased as metabolizable energy density of diet increased from 2650 to 2850 Kcal/kg DM, but it is not affected by increasing crude protein level from 16 to 18%. The average daily gains were not improved (P0.05) as the crude protein levels and metabolizable energy density of diet increased. Crude protein levels and ME density did not affect (P0.05) the DM, OM and energy digestibility, but NDF digestibility was affected by the ME density of diets (P0.05). Daily N intakes were greater (P0.0%) in goats received diets higher in the crude protein and metabolizable energy levels. At this high rate of feed intake this type of goats are able to gain optimally when offered feed with crude protein level of 16% and metabolizable energy density of 2850 Kcal/kg DM
Performance of dairy calves fed diet containing Ca-palm oil fatty acid and Sapindusrarak fruit
Get PDFCalcium salts of palm oil fatty acid (Ca-FA) is a high dense energy source that is protected from degradation in the rumen. Sapindusrarak fruits (SrF) contain high level of saponin and have been reported to increase body weight sheep or cattle and reduced protozoa population in the rumen. This experiment used a combination of Ca-FA and SrF to improve the performance of weaned dairy calves. Thirty two heads of weaned Indonesian Holstein were used in factorial block design experiment (2 x 2). The first factor was Ca-FA(with 2.5% and without addition of Ca-FA) while the second factor was SrF (with 0.3% and without addition of SrF in total diet) and sex of the animal as block was applied. The feed as total mixed ration contained crude protein (CP)17%, total digestible nutrien (TDN)minimum 69%, Net energy for maintenance 1.70Mcal/kg and Net energy for gain 1.03Mcal/kg. It was fed 3% of body weight of the animals for 14 weeks included 2 weeks of adaptation period. Feces collection for measuring digestibility of feed was conducted at the end of experiment. Average daily gain (ADG) of calves received SrF was higher than without SrF (896.9 vs 853.7 g/day) while ADG received CaFA was lower than without CaFA (860.6 vs 890 g/day) but both effects were not significantly different (P0.05). DM intake due to SrF treatment tended to be higher than control treatment (4.4 vs 4.3 kg/day). DM digestibility was not different among treatments. In conclusion,calves received supplementation of 0.3% SrF fruit in the diet had similar average daily gain with those given unsupplemented diet but had reduced diarrhea cases
Evaluation on performance of some Sorghum bicolor cultivars as forage resources in the dry land with dry climate
Get PDFThe aim of this study was to evaluate the performance of several Sorghum bicolor cultivars as forage on a dry land with pH of 5.4, N of 0.08%; C/N of 9%, P of 0.06% and K of 0.01%. Nine cultivars of S. bicolor (Super 1, Super 2, Numbu, Kawali, G2, G5, PAC 537, PAC 593 and PAC 501) were evaluated. Plot size was 16 m2 with space planting of 15 x 75 cm. The experimental design used was randomized block design with three replications. Parameters observed were plant height, time of flowering, forage production and quality. The result showed that the primary plant growth was not different in all cultivars. In the 65 days old primary plant, the Super 2, PAC 537 and Kawali had no flower yet. Biomass production varied in primary plant between cultivars of 11.35 - 26.17 kg/16 m2. The highest biomass production was obtained in PAC 537 of 26.17 kg/16 m2 (16.34 t/ha) which were significantly higher than G2 of 11.35 kg/16 m2 (7.09 ton/ha) and was not significantly different with other cultivars. In the 45 days ratoon I, Super 2, G5 and Super 1 showed faster growth. Biomass production increased in the ratoon I around 19.88 kg/16 m2 (12.42 ton/ha). PAC 537 produced the highest biomass of 30.14 kg/16 m2 (18.84 ton/ha) and was not significantly different with other cultivars, except with the G2. Biomass production of ratoon II decreased around 1.83 kg/16 m2 (1.14 t/ha)–4.77 kg/16 m2 (2.98 t/ha) and increased in the ratoon III of 15.72 kg/16 m2 (9.82 t/ha)–26.05 kg/16 m2 (16.28 t/ha). The quality of forage ratoon I was better compared to the primary plant with the lowest one was in ratoon II. It could be concluded that Super 1, Super 2 and PAC 537 cultivars might be recommended as potential forage
Effectiveness of DMSO concentration on recovery rate and viability of primordial germ cell of Gaok chicken
Get PDFRecent technological developments to produce germ line chimeras with primordial germ cell (PGC) transfer into the recipient embryo provide an opportunity to conserve and retrieval of chicken genetic resources in complete form. The study was conducted to obtain the most effective DMSO percentage to recovery rate and viability of Gaok chicken PGC after freezing which will later be feasible to be transferred. In this study, the eggs of Gaok chicken were incubated for about 2.5 - 3 days to obtain embryos at stages 14 - 16. Blood retrieval was done through the dorsal aorta using micropipettes under microscope. The procedure of PGC isolation of Gaok chicken with centrifugation gradient was using nycodenz as a substance. Commercially available cryoprotectants (dimethyl sulfoxide = DMSO) were used for PGC freezing. Isolated and frozen PGCs of Gaok chicken were diluted with cryoprotectants containing 2.5; 5 and 10% DMSO in fetal bovine serum (FBS). The recovery rate of 2.5; 5 and 10% DMSO concentration were 36.4; 48.2 and 48 % respectively. The viability of PGC after freezing was significantly higher for 5% DMSO compared with 2.5% DMSO (P0.05), but not different from 10% DMSO. It can be concluded that the concentration DMSO of 5 % was effectve contration in freezing Gaok chicken PGC
Anticoccidial activity of Artemisinin and Extract of Artemesia annua leaves in chicken infected by Eimeria tenella
Get PDFThe continuous use of anticoccidial drug in chicken often continuously generates drug resistance and tissue residue; so thatconsequently, a safe alternative anticoccidial drug based on herb is fundamentally required. The aim of thise study was to examine anticcocidial activity of artemisinin and extract of Artemesia annua leaves in chicken infected by Eimeria tenella. A total of 35 chickens of Cobb strain was divided into seven groups with five replicates birds per group, i.e. uninfected chicken group (P I), infected but untreated chicken group (P II), infected and treated chicken group with 8.5 ppm, 17 ppm, 34 ppm, for P III, P IV and P V respectively, infected and treated chicken with 17 ppm of A. annua extract (P VI) and infected and treated chicken with Sulfa (P VII). All chicken, except the uninfected group, whereas infected with 2000 infective oocyst of E. tenella except the uninfected group. Treatment was delivered by oral, once per day for eight days. The criteria observed were clinical manifestation of chickens, number of oocyst in feces, body weight, cecal lesion score, haematocrit (packed cell volume) and haemoglobin value. The results showed that extract of A. annua leaves (P VI) was the most effective treatment to reduce the number of oocyst in feces (74.18%), followed by 34 ppm of artemisinin group (P VII). In addition, application of A. annua extract and artemisinin was significantly able to decreased the cecal lesion score (P0.05). Even though body weight and Hb value were not indifferent significantly different (p0.05), however A. annua extract and artemisinin treatments were significantly able to hold PCV value on normal level compared to P II and P IV (P0.05). It concluded that extract A. annua leaves and artemisinin could be used an alternative anticoccidial in chickens
Plant extract activities as antioxidant and antibiofilm against chicken gut bacteria
Get PDFThe occurrence of microbial resistance against antibiotic due to the subtherapeutic dosage of antibiotic growth promoter (AGP) in poultry can be prevented by the antibiofilm substance. Plant secondary compounds have some activities like antioxidant, antimicrobial, and antibiofilm. This research was conducted to obtain the plant with the highest activity of antibiofilm and also antioxidant by analyzing several plant secondary compounds as antioxidant and antibiofilm against chicken’s gut bacteria. The tested plants were clove leaves, leaffruit plants, mangosteen peel, cashew nut shell, guava leaves, and bay leaves. These plants were extracted with methanol or n-hexane using sonication method. The antioxidant activity as the IC50 value of the plant methanol extracts were determined using α,α-diphenyl-β-picrylhydrazyl (DPPH) assay. The biofilm inhibition activity was tested against Escherichia coli, Salmonella enteritidis, and Staphylococcus aureus ATCC® 29213TM using methanol and n-hexane extracts. All of the samples had antioxidant activity. The clove leaves and leaffruit plants had the highest antioxidant activity, while mangosteen peel extract in methanol had the highest antibiofilm activity against all tested bacteria. The species of bacteria also affected the antibiofilm activity. E. coli and S. enteritidis were more resistant to antibiofilm then S. aureus. Mangosteen peel extract which showed high antioxidant and antibiofilm activity is potential to be used as a feed additive to control the pathogenic bacteria
Application of BS4-enzyme on the methane production from mixture of cattle manures and waste paper
Get PDFCellulose from abundant newspaper waste could be transformed into methane through anaerobic fermentation. This research was carried out to compare the gas production including methane between samples containing feces and waste paper mixture as inoculum and substrate, respectively and added with and without BS4 enzyme. The enzyme was produced in Indonesian Research Institute of Animal Produce (IRIAP) by growing Eupenicillium javanicum BS4 in coconut meals. There were three treatments, i.e., 30% manure (M30); 15 % manure + 15 % paper waste (MP 30); MP30 + 3 mL BS4 enzyme equal to 0.42 U/g dry matter (MPE30) The percentage of waste papers addition in feces was calculated on dry matter (DM) basis and every treatment had five replications. Total gas and methane productions were measured weekly, while dry matter losses were determined during 5 week fermentation. Interactions between treatments and incubation time were analyzed using completely randomized design each week. Kind of substrates influenced both total gas and methane productions during incubation time. Both waste papers and enzyme addition enhanced gas production. The highest total gas and methane productions for five weeks incubation were highly significantly observed (P0.01) in MP30 and MPE30 compared to M30. Addition of enzyme significantly increased total gas and methane productions in the first week. The highest methane and total gas yield/g dry matter were obtained by BS4 enzyme addition. It was concluded that BS4 enzyme is good in accelerating and increasing the transformation efficiency of waste paper and manure mixture for biogas production.
Effect of supplementation of BS4-enzyme levels in rice-bran based rations on performance of growing PMp broiler duck
Get PDFThe purpose of enzymes supplementation in feeds is to improve nutrient digestibility through degradation of anti-nutrition and crude fiber, which are commonly found in rice bran. The aim of the study was to see performance response of PMp broiler ducks to the supplementation of BS4-enzyme levels in rice-bran based rations. Two hundred and twenty four day-old ducks were allocated to 8 dietary treatments with 4 replicates, consisted of 7 ducks in each replicate. The composition of the feed treatments arranged as follows: T1 to T4 were rations with 30% of rice bran content with enzyme levels of 0, 50, 100, and 150 Unit/kg rice bran respectively. T5 to T8 were rations with 60% of rice bran content with the same enzyme levels as for T1 to T4 treatments. The ducklings were subjected to the treatments for the first four weeks. The variables observed were feed intake, weight gain and FCR. The results showed that the supplementation of BS4-enzymes on rice-bran based rations significantly affected (P0.05) feed intake and FCR, but not for weight gain (P0.05). The most effective rations for feed consumption and FCR of PMp broiler duck were obtained on T4 treatment resulting in the highest body weight gain of 998 g/bird and lowest FCR of 2,64. It was concluded that the supplementation of 150 Unit/kg of BS4 enzyme in 30% rice-bran diet was the best combination level to be implemented in feeding PMP broiler ducks for the first four weeks period
Productivity of Brachiaria decumbens in a Mixed-Culture with Pueraria phaseloides in Different Manure and Dolomite Administration Level into Suboptimal Land
Get PDFProductivity improvement of forage in suboptimal land can be done in various ways, such as through manure and ameliorant treatment. Manure and dolomite is required by soil on suboptimal land, a combination of both can supply nutrients and improve soil biophysics. The research materials used in this study were B. decumbens, Pueraria phaseloides, manure, and dolomite. Randomized Block Design (RBD) in 3x3 factorial was applied. The dose of manure and dolomite are the first and second factors respectively, each treatment has 3 replications times. Variables measured were biomass production, dry matter production, carrying capacity, and nutrient content of forage. Results showed that the highest (P0.05) biomass production produced by the 10 t/ha manure and 2 ton/ha dolomite treatment by 1,638.94 g/m2 compared to the other treatments, and the lowest was achieved in the control by 543.75 g/m2. Carrying capacity of B. decumbens and P. phaseloides on 10 ton/ha manure was the highest (P0.05) by 0.08 AU compared to the other treatments. Super dolomite administration by 1 ton/ha significantly (P0.05) produced the highest carrying capacity by amount of 0.83 AU. Manure and dolomite administration significantly (P0.05) improved the nutritional content of B. decumbens and P. phaseloides