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    Kontribusi pendapatan usaha ternak sapi potong rakyat pada pola usahatani terpadu

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    Kontribusi pendapatan usaha ternak sapi potong rakyat pada pola usahatani terpad

    Dampak krisis moneter terhadap daya saing agribisnis ayam ras petelur ditinjau dari sumberdaya domestik

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    Dampak krisis moneter terhadap daya saing agribisnis ayam ras petelur ditinjau dari sumberdaya domesti

    Meat Characteristic of Crossbred Local Chicken Fed Inulin of Dahlia Tuber and Lactobacillus sp.

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    This study was conducted to evaluate the meat characteristic of crossbred local chicken fed diet containing both dahlia tuber powder as inulin source and probiotic Lactobacillus sp. The experimental animals were 168 crossbred local chickens which were randomly divided into 6 groups of treatment (4 replications each) when they were 21-d old. A completely randomized design with 2 x 3 factorial pattern consisted of 2 levels of prebiotic [(0.8% (D1) and 1.2% (D2)] and 3 levels of probiotic [without probiotic (L0), 1.2 mL (L1), and 2.4 mL (L2)] was arranged in the present study. One mL probiotic (Lactobacillus sp.) was equal to 108 cfu. Results showed that the supplementation of prebiotic and probiotic significantly (P<0.05) affected breast meat color in terms of L* (lightness) and b* (yellowness). The meat fat mass and cholesterol was significantly (P<0.05) decreased by the combination of prebiotic and probiotic. The hardness of meat was not affected significantly by all treatments. The conclusion is that breast meat color could be improved, and both meat fat mass and cholesterol content could be decreased by feeding a combination of 1.2% dahlia tuber powder as inulin source and 1.2 mL probiotic Lactobacillus sp

    Pertumbuhan persilangan pelung X kampung pada pemeliharaan intensif

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    Pertumbuhan persilangan pelung X kampung pada pemeliharaan intensi

    Nutrient Content, Protein Fractionation, and Utilization of Some Beans as Potential Alternatives to Soybean for Ruminant Feeding

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    This experiment aimed to determine nutrient content, protein fraction, and in vitro rumen fermentation of some alternative beans in comparison to soybean. Samples used were napier grass, soybean, redbean, groundnut, pigeonpea, cowpea, bambarabean, and mungbean. Samples were determined for their proximate composition, Van Soest’s fiber fraction, and Cornell protein fraction. The samples were subsequently evaluated for their fermentation characteristics and digestibility by using a two-stage in vitro rumen fermentation technique, maintained at 39 oC for 2 × 48 h. The in vitro incubation was performed in three consecutive runs by following a randomized complete block design in which each sample per run was represented by four fermentation tubes. Results revealed that all experimental beans contained high crude protein (CP), i.e. above 200 g/kg dry matter (DM), but only soybean and groundnut had CP contents higher than 300 g/kg DM. Redbean had the lowest crude fiber and acid detergent fiber contents among the beans. Soybean contained high proportion of rapidly degraded CP fraction, but low in slowly degraded and unavailable CP fractions. High proportion of slowly degraded CP fraction was found in redbean and bambarabean. Redbean, pigeonpea, cowpea, and mungbean were better than soybean, groundnut, and bambarabean with regard to DM degradability and DM digestibility values (P<0.05). Concentration of total VFA was the highest in the incubation of redbean. It was concluded that groundnut, redbean, pigeonpea, cowpea, and mungbean have the potency to be used to substitute soybean for ruminant feeding

    Blood Profile of Quails (Coturnix coturnix japonica) Fed Ration Containing Silkworm Pupae (Bombyx mori) Powder Extract

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    The aim of this research was to assess the use of silkworm pupae (Bombyx mori) powder extract on the blood cells and hematological variables of quails (Coturnix coturnix japonica). The treatments consisted of R0= isoenergy and isoprotein ration without pupae powder extract, R1= isoenergy and isoprotein ration + 1% of pupae powder extract, R2= isoenergy and isoprotein ration + 10% of pupae powder extract, R3= isoenergy and isoprotein ration + 1% of residue of pupae powder extract, R4= isoenergy and isoprotein ration + 10% of residue of pupae powder extract, R5= isoenergy and isoprotein ration + 1% of pupae powder, and R6= isoenergy and isoprotein ration + 10% of pupae powder. The variables measured were blood cell and hematological profiles including the number of leukocytes, erythrocytes, and leukocytes differentiation. Immune response was observed at 12, 24, and 48 h by counting the white blood cells type. The results showed that feed consumption ranged 79.19-154.70 g/quail/wk and the body weight was 71.45-149 g/quail. The addition of pupae extract at a dose of 10% in the diet significantly (P<0.05) increased the number of quail white blood cells. The addition of pupae extract, pupae powder residue, and pupae powder in the ration did not significantly affect the number of quail erythrocytes, hemoglobin, and PCV (packed cell volume). Mean number of white blood cells increased during 24 h after treatment of 10% pupae powder extract (R2) and decreased at the 48th h. It is concluded that the value of leukocytes, lymphocytes, monocytes, and heterophile increased in quails supplemented with 10% pupae powder extract (R2)

    Development of Fermented Mare’s Milk Using Mixed Probiotic Cultures

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    Mare’s milk has good nutrient composition for human being in the form of natural milk or milk products. It can be used to replace cow’s milk, especially in the regions outside of Java Island which are rarely found dairy cow. This study had an objective to develop fermented mare’s milk by using mixed cultures of probiotic bacteria i.e. Lactobacillus acidophilus (A), Bifidobacterium longum (B), and Lactobacillus casei (C). The cultures of two probiotic bacteria AB and BC had been developed as well as three probiotic bacteria ABC culture. The mixed cultures (AB, BC and ABC) were prepared in single culture then cultivated in mixed culture as total 10% v/v of mare’s milk used and was incubated at 39°C for 9 h. The pH, acidity and bacterial count, each of them was analyzed in every 3 h of incubation time, while the organic acid and sensory tests were conducted at the end of 9 h of incubation. The results showed that the growth of mixed probiotic bacteria culture BC and ABC had better pH value that was around 3 compared with AB around 4.5. This showed that the cooperation between bacteria was different in each different combinations. The number of bacteria also increased sharply between 3-6 h of incubation time and 6-9 incubation time (AB and BC) along with the increased lactic acid, but the acetic acid decreased from 1750 mg/L to 1500 mg/L. The result of sensory test showed low acceptability of trained panelists. It is concluded that mixed cultures, using two or three probiotic bacteria (L. acidophilus, B. longum, and L. casei) could grow in mare’s milk. The change of the biochemical patterns indicated a commensalism cooperation among bacteria used. It was therefore the fermented mare’s milk using these probiotic bacteria were not well accepted by entrained panelists

    Partial Sequencing of 16S rRNA Gene of Selected Staphylococcus aureus Isolates and its Antibiotic Resistance

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    The choice of primer used in 16S rRNA sequencing for identification of Staphylococcus species found in food is important. This study aimed to characterize Staphylococcus aureus isolates by partial sequencing based on 16S rRNA gene employing primers 16sF, 63F or 1387R. The isolates were isolated from milk, egg dishes and chicken dishes and selected based on the presence of sea gene that responsible for formation of enterotoxin-A. Antibiotic susceptibility of the isolates towards six antibiotics was also tested. The use of 16sF resulted generally in higher identity percentage and query coverage compared to the sequencing by 63F or 1387R. BLAST results of all isolates, sequenced by 16sF, showed 99% homology to complete genome of four S. aureus strains, with different characteristics on enterotoxin production and antibiotic resistance. Considering that all isolates were carrying sea gene, indicated by the occurence of 120 bp amplicon after PCR amplification using primer SEA1/SEA2,  the isolates were most in agreeing to S. aureus subsp. aureus ST288. This study indicated that 4 out of 8 selected isolates were resistant towards streptomycin. The 16S rRNA gene sequencing using 16sF is useful for identification of S. aureus. However, additional analysis such as PCR employing specific gene target, should give a valuable supplementary information, when specific characteristic is expected

    Karakteristik Daging Sapi yang Difermentasi oleh Lactobacillus plantarum dan Terfermentasi Alamiah

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    Karakteristik daging sapi yang difermentasi oleh LACTOBACILLUS PLANTARUM  dan terfermentasi alamia

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    DOI: 10.5398/medpet.2016.39.1.fm.

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