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Low-level predation by lytic phage phiIPLA-RODI promotes biofilm formation and triggers the stringent response in Staphylococcus aureus.
Get PDFAn important lesson from the war on pathogenic bacteria has been the need to understand the physiological responses and evolution of natural microbial communities. Bacterial populations in the environment are generally forming biofilms subject to some level of phage predation. These multicellular communities are notoriously resistant to antimicrobials and, consequently, very difficult to eradicate. This has sparked the search for new therapeutic alternatives, including phage therapy. This study demonstrates that S. aureus biofilms formed in the presence of a non-lethal dose of phage phiIPLA-RODI exhibit a unique physiological state that could potentially benefit both the host and the predator. Thus, biofilms formed under phage pressure are thicker and have a greater DNA content. Also, the virus-infected biofilm displayed major transcriptional differences compared to an untreated control. Significantly, RNA-seq data revealed activation of the stringent response, which could slow down the advance of the bacteriophage within the biofilm. The end result would be an equilibrium that would help bacterial cells to withstand environmental challenges, while maintaining a reservoir of sensitive bacterial cells available to the phage upon reactivation of the dormant carrier population.This study was supported by grants AGL2012-40194-C02-01 (Ministry of Science and Innovation, Spain), AGL2015-65673-R (Program of Science, Technology and Innovation 2013-2017), GRUPIN14-139 (FEDER EU funds, Principado de Asturias, Spain). L.F. was awarded a “Marie Curie Clarin-Cofund” grant (ACB14-01)
Calcium looping CO2 capture system for back-up power plants
Get PDFThis paper analyses a CO2 capture system based on calcium looping, designed for power plants that operate with very low capacity factors and large load fluctuations, including shut-down and start-up periods. This can be achieved by decoupling the operation of the carbonator and calciner reactors and connecting them to piles filled with CaO or CaCO3. When the power plant enters into operation, calcined solids are fed into the carbonator to provide the necessary flow of CaO for capturing CO2 and storing the carbonated solids. An oxy-CFB calciner designed to have a modest thermal capacity and operate continuously refills the CaO pile. Mass and energy balances of the entire system, combined with state-of-the-art performance criteria for reactor design, have been solved to identify suitable operating windows. An analysis of the effect of the CaO reactivity of the material stored in the piles indicates that temperatures of around 500–600 °C in the carbonator are compatible with the storage of solids at low temperature (<250 °C). This, together with the low inherent cost of the material, allows large piles of stored material. Electricity costs between 0.13–0.15 per kWhe when forced to operate at low capacity. The proposed concept could be integrated into existing power plants operating as back-ups in renewable energy systems in preference to other CO2 capture technologies that are heavily penalized when forced to operate under low capacity factors
La Segunda Balada de Liszt: Una resolución técnica y pedagógica para jóvenes pianistas.
Get PDFTrabajo fin de másterThe second ballad of Franz Liszt is a work of study appropriate for students of advanced level given the wide range of technical and musical resources that its interpretation requires; on the other hand, it favors an interesting approach to the pianistic literature of the composer and the romantic repertoire. The analysis of this ballad is presented as a complement to an interpretative study of it. As part of this analysis, we propose a technical and musical solution to it that may be useful to future students.La segunda balada de Franz Liszt resulta una obra de estudio apropiado para alumnos de nivel avanzado dado el amplio abanico de recursos técnicos y musicales que requiere su interpretación; por otra parte, favorece un interesante acercamiento a la literatura pianística del compositor y del repertorio romántico. Se presenta el análisis de esta balada como complemento a un estudio interpretativo de la misma. Como parte de dicho análisis, se propone una solución técnica y musical a la misma que pueda ser de utilidad a futuros estudiantes
Variedades de maíz : actualización año 2017
Get PDFEl ensayo de evaluación de variedades de maíz en Asturias se lleva a cabo por separado
en las cuatro zonas edafoclimáticas que son aptas para el cultivo de este forraje:
zona costera occidental, zona costera oriental, zona interior de baja altitud y zona interior
alta, utilizando un diseño experimental en parcela dividida con 4 repeticiones, en el cual
la “parcela principal” representa al ciclo de cultivo (largo, medio y corto) y la “subparcela”
a la variedad a ensayar. El tratamiento estadístico de los datos diferencia entre lo que es
imputable a cada variedad en sí y lo que es consecuencia del “ambiente”, es decir, de
las variaciones derivadas del año climático y de la propia parcela experimental. En cada
campo experimental, el proceso comienza con la preparación del terreno, fertilización,
delimitación de parcelas, siembra de las variedades a ensayar y aplicación de tratamientos
fitosanitarios. La siembra se realiza siempre de forma manual y de forma que las
variedades de un mismo ciclo queden agrupadas
[BCCB] Block Chain for Consumers and Businesses
Get PDFResultados del proyecto BCCBResults of BCCB projec
Gold(I)-Catalyzed Reaction of Ferrocene and Propargylic Esters: Synthesis of Functionalized Ferrocene Derivatives
Get PDFThe reaction of propargylic esters with ferrocene in the presence of gold catalysts provided ferrocene derivatives containing a functionalized vinyl moiety. This ferrocene C-H bond functionalization takes place with complete regio- and stereoselectivity. The reaction is proposed to proceed through an electrophilic substitution in which a vinylgold carbene intermediate arising from a 1,2-rearrangement of the starting propargylic ester would behave as the electrophilic counterpart
Evaluation of autochthonous Saccharomyces bayanus strains under stress conditions for making ice ciders
Get PDFThe product diversification policy being carried out by the Asturian cider industry includes the development
of beverages similar to so-called ice ciders. During apple juice fermentations, yeast cells are
affected by several stress conditions. In this study, 74 Saccharomyces bayanus strains isolated from
Asturian cider, have been analyzed in synthetic media for their growth ability, sugar fermentation capacity
and acetic acid production under the stress conditions that occurr during the production of ice
cider. According to the data obtained 23 strains high proliferation capabilities, sugar fermentation capacity
and low acetic acid production have been differentiated. Ten strains were chosen to produce ice
ciders from apple juice (31.8 ºBrix) at 12 C. The products obtained were characterized by not having
developed the malolactic conversion and their low contents of acetic acid, methanol and ethyl acetate.
Significant differences among ciders were detected for alcoholic degree, total acidity, and contents of
glycerol, pyruvic, fumaric and shikimic acids due to the yeast strain used. A Multiple Correspondence
Analysis showed four strains associated with sensory quality variables. Our results open up the possibility
of using autochthonous S. bayanus strains as starters in the making of Asturian ice ciders.National Institute of Research and Agro-Food Technology (INIA) and cofinanced with ERDF and ESF funds Project RTA-2012-00075-00-00 and RTA-2013-00110-00-0
BVgcSalud: biblioteca virtual del Sistema Sanitario Público Asturiano
Get PDFBVgcSalud is the virtual library of the Asturian Public Sanitary System (SESPA), it is the tool that serves as a support to the healthcare activity, teacher and researcher of health professionals. There are several resources available: access to databases, evidence-based medicine resources, image banks and catalogs of magazines and books, both electronic and paper, from the Network of Libraries of the Health Service. It also offers a series of services: the Document Obtaining Service (SOD), which allows users to request articles that are not accessible to each user's reference library and the Bibliographic Search Service that supports users when they need them. The BVgcSalud has its own search engine, called BuscaBvgcSalud, which searches within the resources contracted by the institution. And, thanks to the implementation of remote access, it allows its users to connect to the library from home or from any network. All this is accessible from the Astursalud Institutional Portal.BVgcSalud es la biblioteca virtual del Sistema Sanitario Público Asturiano (SESPA), es la herramienta que sirve de apoyo a la actividad asistencial, docente e investigadora de los profesionales sanitarios. Son varios los recursos que ofrece: acceso a bases de datos, recursos de medicina basada en la evidencia, bancos de imágenes y catálogos de revistas y libros, tanto electrónicos como en papel, de la Red de Bibliotecas del Servicio de Salud. También ofrece una serie de servicios: el Servicio de obtención de documentos (SOD) que permite solicitar artículos no accesibles a la biblioteca de referencia de cada usuario y el Servicio de Búsqueda Bibliográfica que apoya a los usuarios cuando lo necesitan. La BVgcSalud dispone de un buscador propio, denominado BuscaBvgcSalud que busca dentro de los recursos contratados por la institución. Y, gracias a la implementación del acceso remoto, permite a sus usuarios conectarse a la biblioteca desde casa o desde cualquier red. Todo ello está accesible desde el Portal Institucional Astursalud
[REAL] Plataforma de Realidad Aumentada integrada en dispositivo visual interactivo para aplicaciones logísticas
Get PDFResultados del proyecto REAL.Results of REAL project
Cisplatin resistance in cell models: evaluation of metallomic and biological predictive biomarkers to address early therapy failure
Get PDFCisplatin, one of the most extensively used metallodrugs in cancer treatment, presents the important drawback of patient resistance. This resistance is the consequence of different processes including those preventing the formation of DNA adducts and/or their quick removal. Thus, a tool for the accurate detection and quantitation of cisplatin-induced adducts might be valuable for predicting patient resistance. To prove the validity of such an assumption, highly sensitive plasma mass spectrometry (ICP-MS) strategies were applied to determine DNA adduct levels and intracellular Pt concentrations. These two metal-relative parameters were combined with an evaluation of biological responses in terms of genomic stability (with the Comet assay) and cell cycle progression (by flow cytometry) in four human cell lines of different origins and cisplatin sensitivities (A549, GM04312, A2780 and A2780cis), treated with low cisplatin doses (5, 10 and 20 mM for 3 hours). Cell viability and apoptosis were determined as resistance indicators. Univariate linear regression analyses indicated that quantitation of cisplatin-induced G–G intra-strand adducts, measured 1 h after treatment, was the best predictor for viability and apoptosis in all of the cell lines. Multivariate linear regression analyses revealed that the prediction improved when the intracellular Pt content or the Comet data were included in the analysis, for all sensitive cell lines and for the A2780 and A2780cis cell lines, respectively. Thus, a reliable cisplatin resistance predictive model, which combines the quantitation of adducts by HPLC-ICP-MS, and their repair, with the intracellular Pt content and induced genomic instability, might be essential to identify early therapy failure.Este trabajo fue patrocinado por el Ministerio de Educación y Ciencia español (MEC, número de protecto CTQ2010-16638); el Ministerio de Economía y Comercio (MINECO, número de proyecto: CTQ2013-39032-C2-1-R); y el Programa Regional de Investigación del Principado de Asturias (número de proyecto: FC-15-GRUPIN14-010). Marta Espina fue patrocinada por una beca FPU (MEC, España) y Mario Corte Rodríguez por la beca predoctoral Severo Ochoa (BP13114, Plan Regional de Investigación del Principado de Asturias, FICYT