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Electrochemical genoassays on gold-coated magnetic nanoparticles to quantify genetically modified organisms (GMOs) in food and feed as GMO percentage
Get PDFThe integration of nanomaterials in the field of (bio)sensors has allowed developing
strategies with improved analytical performance. In this work, ultrasmall core-shell
Fe3O4@Au magnetic nanoparticles (MNPs) were used as the platform for the
immobilization of event-specific Roundup Ready (RR) soybean and taxon-specific
DNA sequences. Firstly, monodisperse Fe3O4 MNPs were synthesized by thermal decomposition and subsequently coated with a gold shell through reduction of Au(III)
precursor on the surface of the MNPs in the presence of an organic capping agent. This
nanosupport exhibited high colloidal stability, average particle size of 10.2±1.3 nm, and
spherical shape. The covalent immobilization of ssDNA probe onto the Au shell of the
Fe3O4@Au MNPs was achieved through, a self-assembled monolayer (SAM) created
from mixtures of alkane thiols (6-mercapto-1-hexanol and mercaptohexanoic acid). The
influence of the thiols ratio on the electrochemical performance of the resulting
electrochemical genoassays was studied, and remarkably, the best analytical
performance was achieved for a pure mercaptohexanoic acid SAM. Two quantification
assays were designed; one targeting an RR sequence and a second targeting a reference
soybean gene, both with a sandwich format for hybridization, signaling probes labelled
with fluorescein isothiocyanate (FITC), enzymatic amplification and
chronoamperometric detection at screen-printed carbon electrodes (SPCE). The
magnetogenoassays exhibited linear ranges from 0.1 to 10.0 nM and from 0.1 to 5.0 nM
with similar detection limits of 0.02 nM and 0.05 nM for the event-specific (RR) and
the taxon-specific (lectin) targets, respectively. The usefulness of the approach was
demonstrated by its application to detect genetically modified organisms (GMOs) in
feed and food.Instituto de Investigación Sanitaria del Principado de Asturias (ISPA
Pinzamiento tardío del cordón umbilical. Hospital Universitario Central de Asturias
Get PDFProtocolo del Servicio de Obstetricia y Ginecología el HUCA. Área Sanitaria I
Focusing aptamer selection on the glycan structure of Prostate-Specific Antigen: toward more specific detection of prostate cancer
Get PDFThe development of chemical sensors capable of detecting the specific glycosylation patterns of proteins offers a powerful mean for the early detection of cancer. Unfortunately, this strategy is scarcely explored because receptors recognizing the glycans linked to proteins are challenging to discover. In this work, we describe a simple method for directing the selection of aptamers toward the glycan structure of the glycoproteins, with prostate-specific antigen (PSA) as a model target. Using this strategy, we identified one aptamer (PSA-1) that binds the glycan moiety of PSA with reasonable affinity (a dissociation constant of 177 ± 65 nM). Interestingly, an electrochemical sensor with a sandwich format employing the identified aptamer as a signaling receptor, provides a tool of discriminating human PSA from the unglycosylated protein, with a limit of detection of 0.66 ng/mL. The sensor responds to different levels of PSA in serum, correlating well with chemiluminescence ELISA used in hospitals even with higher potential to discriminate clinically meaningful prostate cancer. Although validation on a larger cohort is needed, this is the first demonstration of an aptamer-based sensor to detect PSA by focusing in its glycan moiety.Instituto de Investigación Sanitaria del Principado de Asturias (ISPA
Manejo de metrorragia premenopaúsica. Hospital Universitario Central de Asturias
No full textProtocolo del Servicio de Obstetricia y Ginecología el HUCA. Área Sanitaria I
Soldeo robotizado para fabricación de piezas complejas por soldadura aditiva
Get PDFProyecto financiado dentro de la Convocatoria del Programa Asturias de ayudas a Centros Tecnológicos en el Principado de Asturias durante el periodo 2016-2017 de la Consejería de Empleo, Industria y Turismo. Nº de referencia IDI/2016/00029
Análisis estadísticos para determinar el grado de estandarización y las unidades de medida en la producción cerámica
No full textThrough different samples of pottery from European archaeological sites with disparate cultures
and chronologies, this research tries to outline a methodology to determine the standardisation degree of
these samples and to discover the measurement systems used in the production process. The morphometric
characterization of each fragment, allows us to create different groups with clustering analysis. The
principal component analysis provided information that led us to a deeper understanding of the obtained
groups. The coefficient of variation brought us to the standardisation degree of the productions. Once all
these data are known, we can approach the possible units of measure that governed the analysed production
systems.
Keywords:Gobierno del Principado de Asturias, programa “Severo Ochoa” de ayudas predoctorales FICYT.Resumen: A través de diferentes ejemplos de lotes cerámicos -procedentes de yacimientos europeos de culturas
y cronologías dispares- se tratará de esbozar una metodología para estimar el diferente grado de
estandarización de esas producciones y llegar a discernir los sistemas de medida utilizados en su elaboración.
La caracterización morfométrica de cada una de las piezas nos permitirá identificar diferentes grupos
mediante un análisis de agrupamiento (i.e. clustering). El análisis de componentes principales dará información
para entender mejor los grupos obtenidos. El coeficiente de variación nos acercará al grado de
estandarización de las producciones. Una vez que obtengamos dichos datos, podremos investigar las posibles
unidades de medida que rigieron los sistemas productivos de los lotes cerámicos bajo estudio
Archaeometric analysis of Asturian pottery in XVI-XVII centuries
No full textWe offer in this paper the results of the first archaeometric analysis performed on pottery productions elaborate in Asturias (Spain) between the early sixteenth century and the mid-seventeenth century. 10 samples were selected from the most representative types of Asturian potteries from this period. The samples were analyzed using different characterization techniques: X-Ray Fluorescence (XRF), for elementary quantification of the components of the different samples and Glow Discharge Time-of-Flight Mass Spectrometry (GD-ToFMS) to meet qualitatively of minor elements present. The supplied data will allow us to characterize and learn about productions that were made in Asturias in the late Middle Ages from a physical-chemical view.Gobierno del Principado de Asturias, Programa “Severo Ochoa” de ayudas predoctorales (FICYT).Actas del XIth Congress AIECM3 on Medieval and Modern Period Mediterranean CeramicsOfrecemos en este trabajo los resultados de los primeros análisis arqueométricos realizados sobre producciones cerámicas elaboradas en Asturias (España) entre principios del siglo XVI y mediados del siglo XVII. Se han seleccionado 10 muestras pertenecientes a las tipologías más representativas de la cerámica asturiana de esta época. Las muestras han sido estudiadas utilizando diversas técnicas de análisis: Fluorescencia de Rayos X (XRF), para la cuantificación elemental de los componentes de cada muestra y Glow Discharge Time of Flight Mass Spectrometry (GD-ToFMS) para conocer los elementos minoritarios presentes. Los datos aportados nos permitirán caracterizar y conocer desde un punto de vista físico-químico las producciones que se elaboraban en Asturias a finales de la Edad Media
Guía de actuación desde el ámbito de servicios sociales: centros de servicios sociales y unidades de trabajo social
No full textEsta guía alude a la diferenciación de dos ámbitos en los que pueden detectarse situaciones de maltrato a personas mayores y sobre los que procede intervenir de manera diferenciada: Ámbito relacional-domiciliario, aquel que se produce en un entorno de convivencia con otras personas con las cuales puede existir o no vínculo de parentesco. Ámbito institucional: hace referencia a los establecimientos prestadores de servicios y cuidados, a corto o largo plazo, públicos o privados (residencias geriátricas, centros de día, etc
Support Vector Machines to Accelerate Reflectarray Analysis and Optimization
Get PDFThe analysis of reflectarray antennas is substantially accelerated by using Support Vector Machines (SVMs) to model the matrix of reflection coefficients in substitution of a full-wave analysis based on local periodicity, the Method of Moments (MoM-LP) in this work. The SVM model takes as input variables two geometrical variables which control the phase-shift for two different polarizations. Thus, the employed model is able to analyze shaped-beam dual-linear polarized reflectarrays. As test case, a shaped-beam reflectarray radiating a pattern for Local Multipoint Distribution Service applications, presenting a squared-cosecant cut in elevation and sectored-beam in azimuth, is presented, showing a high degree of agreement in both the copolar and crosspolar patterns between the simulations of SVM and MoM-LP. Furthermore, the acceleration with regard to the MoM-LP is between three and four orders of magnitude, which demonstrates the suitability of the SVM model approach for reflectarray direct optimization.This work was supported in part by the European Space Agency (ESA) under contract ESTEC/AO/1-7064/12/NL/MH; by the Ministerio de Economía y Competitividad (Spanish Government), under projects TEC2014-54005-P (MIRIIEM) and TEC2016-75103-C2-1-R (MYRADA); by the Gobierno del Principado de Asturias / FEDER under project GRUPIN14-114; and by the Gobierno del Principado de Asturias through Programa "Clarín" de Ayudas Postdoctorales / Marie Curie-Cofund under project ACA17-0912th European Conference on Antennas and Propagation (EuCAP
Uso y utilidades de la herramienta para la revisión participativa AVANZAR-AGCP
Get PDFInforme que presenta los resultados de la evaluación realizada sobre el uso y aplicaciones de la Herramienta Avanzar en AGCP, un instrumento para la revisión participativa de centros que quieren avanzar en la aplicación de un modelo de Atención Centrada en la Persona