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TRANSFORMASI GEN ARABIDOPSIS ASYMETRIC LEAVES2 (AS2) PADA ANGGREK PHALAENOPSIS AMABILIS (L.) Blume DENGAN MEDIATOR AGROBAKTERIUM TUMEFACIENS
No full textThe Asymmetric leaves2 (AS2) gene is an important gene of a model plant, Arabidopsis thaliana, that involved in leaf development. To understand the molecular mechanism of leaf development in orchid, we transformed the AS2 gene into the Phalaenopsis amabilis orchid protocorms (developing orchid embryos). Agrobacterium tumefaciens LBA4404 that contained T-DNA with 355:: AS2 construct and pGreen vector were used for transformation. The-21-day-old protocorms that co-cultivated with Agrobacterium culture containing pG35S::AS2/LBA 4404 and pG35S/LBA4404 were maintained on 100 mg/1 kanamycin containing medium. The growing plants were selected as candidate of transformants. The putative transformants were analysed by PCR with a pair of AS2 gene specific primers (ORF15 F2 and ORF I 5 R2), that amplifying 600 by fragment of AS2 gene. The result showed that 79 plants out of 7966 protocorms transformant p35S: : AS2 and 64 plants out of 4622 protocorms transformant pG35S were kanamycin resistants, the efficiency of transformations were 0.99% and 1.38%, respectively. Phenotype characterization of the transformation showed that they produced various abnormal leaves shapes: oval (34.18%), wildtype-like (30.38%), rectangular (26.58%), lobed (3.80%), fused (2.53%) and trumpet (2.53%). These data indicate that abnormal shape of leaves might be due to the expressions of AS2 transgene in P amabilis.
DETERMINASI CESIUM-137 DARI AIR LAUT MENGGUNAKAN RESIN HEKSASIANOFERAT
No full textDetermination of cesium-137 in seawater was done using the hexacyanoferrate resin as ion exchanger from 3 point samplings of surface seawater. The hexacyanoferrate ion exchanger matrix was prepared by performing the reaction ofsilica gel supported with potassium hexacyanoferrate and copper (II) chloride. In order to test the performance of the ion exchanger, a batch experiment was performed. A known activity of cesium-137 was used to spike seawater then the hexacyanoferrate resin was added. The separated hexacyanoferrate resin was then counted with gamma spectrometer The cesium-I 37 concentration used in the work varies between 0.5 Bq-20 Bq while the results of the adsorbing efficiency varies between 58-96%. In time variation between 0.5-20 minutes, the adsorbing efficiency varies between 77-96% with highest efficiency at contact time 60 minutes. The hexacyanoferrate ion exchange columns were used to process the Jepara's seawater The calculated cesium-137 activities were 1.37 Bq/m3-2.76 Bq/m3.
PENINGKATAN USAHA EKONOMI TRADISIONAL: STUDI KASUS PETANI MADU HUTAN DI DESANANGA LEBOYAN, KAPUAS HULU
No full textThis research presents the success story of the honey harvesters at Nanga Leboyan Village, Selimbau District, Kapuas Hulu Regency, West Kalimantan Province in improving their traditional economic activity. The aims of the study are to describe changes in techniques and treatments in honey business management and to explain the effects of the changes. Qualitative approach was applied in this study. Data collecting was conducted by applying participant observer and in-depth interview technique. Informants were selected based on snowballing method. The results of this research indicate that the changes in techniques and treatments in honey business management bring about positive impacts for the honey harvesters. The effects are: (1) Increasing of honey quality that cause increasing in the product price. (2) Mortality of honey bees becomes less that lead to increasing amount of honey that can be produced.
HUBUNGAN ANTARA KEMAMPUAN BERBICARA BAHASA INGGRIS DENGAN HASIL TOEFL PADA TARUNA SEKOLAH TINGGI ILMU PELAYARAN (STIP) JAKARTA
No full textThe purpose of this research was to describe the correlation between speaking ability and TOEFL (Test of English as a Foreign Language) score of cadets in Sekolah Tinggi Ilmu Pelayaran (STIP) Jakarta. The research was conducted in STIP because STIP has declared to produce internationally qualified seafarers. In line with its declaration, STIP always evaluates the cadets' English proficiency by Speaking test and TOEFL (Paper-Based TOEFL) every semester Population of this research was the first year cadets, batch 50. The respondents consisted of 20 cadets every major, so there were 60 cadets altogether. The instruments used in this research were interview form and TOEFL question set. This research showed that there was low positive correlation between speaking ability and TOEFL score. The correlation coefficient was 0.276. Therefore, it can be concluded that there are other factors influence TOEFL score. They are readiness, experience, and strategy.
METODE PEMBUKTIAN KESAHIHAN HADIS
No full text Everyone who will convey Tradition of The Prophet (hadits) must check his integrity so that not everybody can convey hadits. Appearance fake haditses push hadits experts to specify a method for proving hadits authenticity, by checking sanad (the transmission of hadits) and matan (text of hadits). Sanad and matan are two fundamental elements on every Hadits. There is a very tightly bearing that cannot be disassociated between sanad and matan. One saying can be called as Hadits, if founded the existence of transmission of rawi (transmitterr) which waschronically up to the first source (Prophet Muhamad). On the contrary, the network of carriers, though to Prophet, without existence of message (text) which informed can not be called as Hadits. Existence of method for proving hadits authenticity is expected to become reference for everyone wishing to convey hadits correctly.