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The Growth of Jackfruit (Artocarpus heterophyllus L.) Shoots on Various Concentration Benzylamino Purine (BAP) in Vitro
Get PDFThe development of Central Sulawesi superior commodity,which is Tulo-5, the drough-resistant cultivars, through tissue culture technique can be used as an alternative, in addition to conventional breeding. This study aims to determine the growth of jackfruit shoots in various concentrations of benzylamino purine in vitro. The research has been conducted at the Laboratory of Plant Biotechnology, Agriculture Faculty of Tadulako University, Palu. The research began on December 2017 and ended up on February 2018. This research used Completely Randomised Design of one-factor treatment which is BAP concentrations consist of 4 stage which is1,5 ppm BAP, 2,0 ppm BAP, 2,5 ppm BAP, 3,0 ppm BAP. The result showed that 6 weeks after planting, BAP concentration had significant effect on shoots time to emerge and shoots number but no significant effect on leaf number. The result of Tukey’s HSD test showed that concentration2,5 ppm gave the fastest shoot emergence with an average of 2,875 days after planting, in concentration 2,0 ppm gave the highest number of shoots with an average of 2.125 shoots per explant, and for the highest number of leaves found in the concentration of 2,0 ppm with the average number of leaves is 1 strand per explant. 
Competitiveness of Soybean Business Farming Analysis in Grobogan Regency Central Java Province
Get PDFSoybean is one of the strategic commodities. To fulfill the increasing of domestic demand, it must be supplied from imports because domestic production is unsufficient. In addition, lower price of imported soybean has effected in competition of domestic farmers to get profit. Demand of soybean in the international and domestic market has always increased. Indonesia has imported soybeans to fill domestic soybean demand. This condition will have an impact on the decline in farm income due to soybean import. The aims of this study were to analyze the level of private profitability and competitiveness of soybean farming in Grobogan Regency.This research used survey research method with number of samples taken through simple random sampling of 100 soybean farmers. Data were analyzed by Policy Analysis Matrix (PAM). The results showed that private profitability of soybean farming in Grobogan Regency was Rp1.690.393,22 per MT. Two indicators to measure the competitiveness were Private Cost Ratio (PCR) and Domestic Resources Cost Ratio (DRCR). PCR value was 0,75 which showed that soybean farming in Grobogan Regency was more competitive and Domestic Resources Cost Ratio (DRCR) showed 0,88. It showed that soybean farming had a comparative advantage
Antibacterial and Antioxidant Activities of Cocoa Pod that Associated in Maltodextrin in Various Concentration
Get PDFCocoa pod extract ((Theobroma cacao L.) has antioxidant and antimicrobial activity that has the potential as a natural food preservative. However, in its use the cocoa fruit skin extract has a disadvantage because the short shelf time and its application to food are limited, efforts are needed to prevent damage and extend shelf life, one of the efforts that can be done is by encapsulating the extract.This study aims to determine the antibacterial activity and antioxidant encapsulation of cocoa peel extract, this study begins with the extraction of cocoa pods with ethanol solvent by comparing cocoa pods : solvent 1: 4 The skin of cacao cocoa fruit used is yellow harvested cocoa fruit, then chopped and dried to form flour.The sample is extracted by maceration with ethanol solvent Antioxidant test is done by DPPH method, while antibacterial test is carried out by the well diffusion method. This study used a completely randomized design method (CRD) with 5 treatments using a maltodextrin concentration of 20% (M1); 30% (M2); 40% (M3); 50% (M4) and 60% (M5). The results showed that the treatment gave the highest yield in the treatment of 60% maltodextrin concentration (M5), while the highest antioxidant activity was obtained in the treatment of 20% maltodextrin (M1) with IC50 75.98 µg / mL and the treatment with the lowest antioxidant activity was obtained at treatment of 60% maltodextrin concentration (M5) with IC50 value 114.89 µg / mL. While for the antimicrobial activity also obtained with the same results, namely treatment of 20% (M1) obtained a higher inhibition diameter compared to treatment at 30%; 40%; 50% and 60% for all types of bacteria. The inhibition diameter in the treatment of the concentration of maltodextrin 20% (M1) for E. coli bacteria is between 4.12 mm - 10.95 mm, Salmonella sp is 2.85 mm - 8 , 25 mm and for Staphylococcusaureus of 5.15 mm - 13.90 mm and the lowest inhibition diameter was obtained in the treatment of 60% maltodextrin concentration (M5) for E. coli bacteria of between 2.0 mm - 4.79 mm, Salmonella sp of 1.15 mm - 4.35 mm and for Staphylococcusaureusat 2.76 mm - 5.17 mm.This study concluded that the encapsulation of cocoa peel extract using 20% maltodextrin had the highest antioxidant and antimicrobial activity when compared with other treatments namely 30% concentration; 40%; 50% and 60% but for the treatment of 20% and 30% there is no difference. Ethanol extract of cocoa pods can be made in the form of encapsulates which are very likely to be used as natural preservatives
Effect of Immersion Time of Shallot Extract and Atonicon Seed Germination of Shallot
Get PDFThe need for raw materials of fried onions derived from \u27lembah palu Shallot tubers variety is high enough and can not be fulfilled, because the productivity is still low. This study aimed to find the type of plant growth regulator substances (PGR) with a long period of proper immersion to improve the germination of Shallot tuber. The research was conducted on Mei until June 2017 in Bulupontou Village, Sigi Regency. The study used two factorial Randomized Block Design (RBD). First factor of type RGS: (Z1) onion extract 100 g/l water, and (Z2) atonic 5 ml/l water. The second factor was long seed immersion in PGR: (T1)=30 min; (T2)=60 minutes; (T3)=90 minutes, and (T4)=120 minutes. Each treatment was represented 20 plants and repeated 3 times, so it was used 480 plants. The results showed that (i) red onion tubers soaked with Shallot extract 100 g/liter of water for 30-90 minutes yielded 100% Germinations, and tubers soaked in red onion extract for 30 minutes resulted in germination rate 31.3%/etmal, (ii) the type of growth regulator of shallot extract and atonic (PGR) effect was not significant on the germination of shallot; and (iii) the 30 minute long growth regulator immersion produced the highest hypothetical vigor index
PHYSICOCHEMICAL AND MICROBIOLOGICAL CHARACTERISTICS OF BEEF MEATBALLS IN PALU CITY
Get PDFThe aim of the study is to evaluate the physicochemical and microbiological characteristics of beef meatball produced and circulated in Palu City. The study used a completely randomized design with 8 micro, small and medium enterprises (MSMEs) where sampling was repeated three times to obtain 24 research samples. Parameters of analysis included water, ash, protein, fat, borax, formalin and total microbial content. The results show that beef meatball circulating in Palu City had water content (66.56 - 72.99%), ash content (1.28 - 1.99%), protein content (8.93– 10.76%). ), fat content (1.16 - 5.84%) and total microbes (1.92–7.97 log cfu / g) and borax content and beef meatball formalin showed negative values
The Effect of Application of Rice Dishwater and Manure as Organic Fertilizer to the Growth of Mustard (Brassica Juncea L.)
Get PDFOne of the recommended concepts of organic agriculture is the use of organic fertilizers and minimizing the use of inorganic fertilizers in farming activities or crop cultivation. Manure is one of the organic fertilizers that are often used as fertilizer, one of which is goat manure. In addition, there are several types of household waste that can be used as liquid organic fertilizers such as the waste rice washing water that can be applied to plants. The research objective were to determine the effect of giving rice washing water waste and manure to the growth and yield of mustard plants. This research was conducted from February to April 2017. This study used a randomized block design with two factors consisting of 16 treatments and repeated three times. The first factor isto control rice washing waterwith a concentration of 10, 20 and 30 ml/liter of water and the second factor is manure consisting of control, manure 5, 10 and 15 grams. This study uses quantitative data analysis techniques. Analysis of quantitative descriptive data used is plant height and number of leaves.Observation results of plant height showed that the highest average of plant height was experienced by 20 ml/liter rice washing water and 15 goat manure treatment. Likewise, the average number of leaves of mustard plants was not significantly different from the treatment of rice washing water, but the number of leaves in mustard plants was not significant. The results showed there were no significant interactions between the concentration of rice washing water and manure doses on growth and yield of mustard greens The rice washing water did not have a significant effect on plant height and number of leaves. The difference in the results of this study was thought to be caused by differences in the types of rice used
The Growth of Kiwi Shoot (Actinidia deliciosa) on Various Kinds of Gelling Agents
Get PDFKiwi fruit takes about 25 weeks from flower bloom until it reaches physiological maturity, so the time required to produce kiwi seeds from seeds in large quantities and uniform is very long. Tissue culture is one method that can be used to obtain a lot of kiwi seeds and uniforms with large quantities in a faster time. The purpose of this study was to examine various types of media compaction materials for the growth of kiwi shoots in vitro. This study was prepared based on Completely Randomized Design (RAL) with 5 treatments and repeated 4 times so that there were 20 experimental units, each experiment using 2 explants so that there are 40 eksplan. The treatments were: MA 1: Agar Swallow Globe 8 g / l, MA 2: Agar Swallow Globe 4 g / l + Agar Nutrijell 4 g / l, MA 3: Agar Swallow Globe 4 g / l + Agar Nutrijell 5 g / l, MA 4: Phytagel 2.2 g / l, MA 5: Agar Nutrijell 11 g / l. Observation variables are When shoots appear, Number of shoots, number of leaves, Number of Roots, number of root hair. The results showed Swallow Globe 4 g / l + Agar Nutrijell 4 g / l treatment gave the highest average number of shoots, the highest number of leaves and roots, this proved that the combination of Swallow Globe and Nutrijell agar gave a good condition for shoot growth kiwi plant
Initiation of Grape (Vitis vinifera L.) on Different Sterilization Techniques
Get PDFThe most crucial step during the development of tissue culture is the method of explant sterilization. Especially, explants is sourced directly from fields that were more susceptible to microbial contaminations. This study aimed to obtain sterilization technique from several sterilant for reduced contamination and support growth of shoots grape in vitro. This research was conducted at the Laboratory of Plant Biotechnology, Faculty of Agriculture, University of Tadulako, during January to April 2018. The study used a factorial completely randomized design, one factor was tested i.e S1 = Taft 8,3 g/L (0,1% carbendazim), S2 = taft 25 g/L (0,3% carbendazim), S3 = taft 41,67 g/L (0,5% carbendazim), S4 = Taft 8,3 g/L (0,1% carbendazim) + HgCl2 0,1%, S5 = Taft 25 gr/L (0,3% carbendazim) + HgCl2 0,1% dan S6 = 41,67 gr/L (0,5% carbendazim) + HgCl2 0,1 %. There were six treatment and each treatment was replicated three times to obtain 18 experimental units. Each experimental unit using two explant. Data were analyzed using analysis of variance and followed by Honestly Significant Difference test at level of 5% if the treatment effects were significant. The results showed sterilization technique using taft 41,67 gram / L containing 0.5% carbendazim for 30 minutes, followed by 70% alcohol for 30 seconds and HgCl2 0,1% for 10 minutes gave free contamination for explant.whereas, Taft 8.3 g / L (Carb 0.1%) for 30 min, followed by 70% alcohol for 30 seconds is a sterilization technique that does not inhibit explant growth as indicated from the most callus formation
Waste Application of Seaweed (Eucheuma Cottonii) on Plant Growth and Results of Mustard (Brassica juncea L.)
Get PDFSeeing the importance of consuming vegetables and increasing of public demand each day, to increase the maximum production using the fertilizer is one of the solutions, either organic fertilizers or inorganic fertilizers. The objective of this study is to determine the effect of seaweed Eucheuma cottonii waste application on growth and yield of mustard. This study was conducted on January and February 2016. Located in Sidondo, district torch and Seed Technology Laboratory Sciences, Tadulako University, Palu, Central Sulawesi. This study was conducted using a randomized block design (RBD), with treatment consisting of R0 (control), R1 (20% of waste seaweed), R2 (40% of waste seaweed), R3 (60% of waste seaweed), R4 (80% of waste seaweed) and R5 (100% of waste seaweed). Each treatment was repeated four times so that there are 24 experimental units. The results showed that the treatment of waste RO seaweed significantly affect the growth and yield of mustard. R5 (100% of waste seaweed) generating plant height, leaf number, fresh weight, dry weight, better than other treatments
The Effect of Soil Water Content and Gamal Bokashi on Vegetative Growth of Sweet Corn (Zea Mays Saccarata)
Get PDFThis research aimed to get the moisture content and dose of bokashi gamal accordance with the vegetative growth of the sweet corn plant (Zea mays saccarata) and the benefit of this study was to provide the information on the effects of agriculture on groundwater levels and bokashi gamal to plants. The research design using factorial completely randomized design. The first factor bokashi gliricidia with 3 levels of treatment was without bokashi gamal (B0), bokashi gamal 20 t.ha-1(B20), and bokashi gamal 40 t.ha-1 (B40). The second factor levels with 3 levels of water treatments, namely, water content 50% field capacity (K50), the water content of 75% field capacity (K75), and water content 100% of field capacity (K100). Treatment was repeated three times to obtain 27 experimental units. Data analyzed by analysis of variance according to the design used and conducted a further test using Honestly Significant Difference (HSD). The results showed the interaction between water content and Bokashi Gamal significantly affected the vegetative growth of the plants sweet corn. The water content of 50-75% and gamal bokashi 20-40 t.ha-1gave the best to plant vegetative growth of sweet corn