Althea Medical Journal
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Knowledge, Attitude, and Perception about Autopsy among Community in Bandung
Get PDFBackground: Autopsy is a comprehensive evaluation of the body and the circumstances surrounding an individual’s death. Currently, autopsy rates in various countries have been decreased in recent years. This decrease was due to the rejection by people who had inadequate knowledge, attitudes, and perceptions about autopsies. This study aimed to determine the knowledge, attitudes, and perceptions of Bandung residents about autopsies.Methods: This cross-sectional descriptive observational study involved people in the Bandung City who were over the age of 20 during the period August to September 2020. The sample was obtained using a snowball system. Research subjects filled out a questionnaire through a Google form containing questions about knowledge, attitudes, and perceptions. The results were categorized based on the median for each variable.Results: Subjects with questionnaire data obtained were 378 people with an average age of 30.30±13.48 years, and most (66.9%) were female. A total of 259 people had adequate knowledge (68.5%), 254 people (67.2%) had a positive attitude, and 249 people (65.9%) had a positive perception, with a median value of 7, 6, and 12, respectively. However, 95 people did not agree to do an autopsy on their relatives or family to determine the cause of death.Conclusion: The subject as a whole showed adequate knowledge, positive attitude, and perception. However, there are still some people who show inadequate knowledge and poor attitudes and perceptions about autopsies. It is recommended that the government and other institutions intervene in the community to increase knowledge and attitudes and improve public perception
Snakebites and the Effect of Serum Anti Bisa Ular (SABU) Antivenom at Dr. Hasan Sadikin General Hospital Bandung, Indonesia: an Overview Period 2015–2019
Get PDFBackground: Snakebite is considered a global health issue, especially in the Southeast Asian region. However, data regarding snakebite cases in Indonesia are still very limited. This study aimed to explore the venomous snakebite cases and the serum anti bisa ular (SABU) antivenom effect in treating venomous snakebite caused by other than the snake species indicated in currently available SABU antivenom formulas.Methods: The analytical descriptive method with a cross-sectional study design was conducted in 2021 using a total sampling from the medical records of 63 patients diagnosed with snakebite at Dr. Hasan Sadikin General Hospital Bandung from 2015 to 2019. Characteristics of patients, clinical manifestation, and correlation between snakes species and outcomes after treatment with or without administration of SABU antivenom were collected.Results: Out of 63 patients, males (79%) were predominant with an average age of 39 years. Fifty-six patients arrived at the Emergency Room less than 24 hours after the occurrence (89%). Most cases were categorized as grade 2 (41.%) where the upper extremities were commonly bitten the body area (64%). Edema (83%) was the most common manifestation. There was no correlation between snake antivenom administered to a specific snake species and the stated outcome (p=0.053), meaning that SABU antivenom might be an effective alternative to treat more types of snakebites.Conclusions: Snakebites are most common in males, attack the upper extremities, categorized as grade 2 with edema. Administration of SABU antivenom provides the similar outcome compared to the group caused by the Javan spitting cobra, Banded krait, and Malayan pit viper
Application of Replicate Organism Detection and Counting Method (RODAC) in Measuring Mycobacterium Tuberculosis Contamination in High Burden Laboratories
Get PDFBackground: Technicians working in high burden tuberculosis (TB) laboratories pose a higher risk of being infected by Mycobacterium tuberculosis from clinical samples. Contamination control is mandatory to detect the release of bacteria into the working environment and to minimize the risk of exposure to the workers. The contamination measurement is rarely performed due to the lack of standard methodology. This study optimized and applied a unique culture-based method named Replicate Organism Detection and Counting (RODAC) plates to assess the presence of M. tuberculosis contaminant in the laboratory with high burden of clinical samples. Methods: RODAC was applied on twenty working surfaces in the Mycobacteriology Laboratory of Universitas Padjadjaran. The results of RODAC were compared with DNA-based detection from the same working surfaces using in-house IS6110 real-time PCR (IS6110-qPCR). The detection limit of the RODAC plate was 19.6 CFU mL-1.Results: From all working surfaces tested, two distinct colonies were found on RODAC plate stamped on the Ziehl-Neelsen staining basin. Those colonies were identified as M. tuberculosis and non-tuberculous mycobacteria (NTM), as confirmed by the MPT64 antigen test and the presence of acid-fast bacilli. IS6110-qPCR detected the presence of M. tuberculosis DNA in ten sampling points, including the ZN staining basin, incubators, and microscopy areas. IS6110-qPCR detected more working surface contamination versus RODAC. However, it was noted that RODAC, which was a culture-based method, detected live bacteria, while PCR could not distinguish between live and dead bacteria.Conclusion: The application of the RODAC plate is more suitable for monitoring the contamination of live bacteria in the working environment and to inform a proper corrective action
Comparison of Single Centrifugation, Double Centrifugation and Turn down-Turn up Techniques for Platelet-Rich Plasma Quality
Get PDFBackground: Platelet-rich plasma (PRP) is a new concept used in medical world, especially for wound healing. The main process that affects the PRP quality is the centrifugation process. This study aimed to assess the PRP separation process and determine the best technique of various centrifugation process.Methods: This experimental study used acid citrate dextrose (ACD) blood taken from 11 healthy respondents and compared three-techniques including the single centrifugation (SC), the double centrifugation (DC), and the double centrifugation turn down - turn up (DC-TDTU) techniques. The quality of PRP was measured based on blood cells count (platelet, leukocyte, erythrocyte count, and Ht value) at each stage of centrifugation. The examination was carried out in 2021 at the Hematology Laboratory, Poltekkes Jakarta 3.Results: The mean values of platelets, leukocytes, and Ht were increased in PRP compared to plasma supernatant both using the DC and DC-TDTU techniques, wherase the SC technique decreased in plasma compared with whole blood. When the procedures using DC and DC-TDTU are carried out properly, platelets would be concentrated in the second centrifugation. However, some erythrocyte and leukocyte contamination occurred by DC-TDTU technique compared to the DC technique.Conclusion: The double centrifugation technique is the best Platelet-rich plasma separation technique compared to the DC-TDTU and SC techniques
Clinical Characteristics of Pediatric with Germ Cell Tumor: Experience in a Developing Country
Get PDFBackground: Germ cell tumor (GCT) occurs in 3% of pregnancy malignancies and increases by 15% during adolescence. In developing countries, the five-year survival rate is 98% and 80% for GCT earlier stage and malignant tumor, respectively. This study aimed to gather information about clinical manifestations of GCT and to evaluate the treatment outcome in pediatric GCT patients at Dr. Hasan Sadikin General Hospital, Bandung, IndonesiaMethods: A descriptive cross-sectional study design with a total sampling method was conducted using secondary data from the medical record of pediatric patients diagnosed with GCT from 2015 to 2018. Data on nutritional status, clinical characteristics, histopathology stage, hematological profiles, chemical markers, treatment methods, completeness of treatment, and outcome at the end of treatment were collected and presented using tables and charts.Results: Of the 44 subjects, girls (77.3%) were predominant, with a mean age of 2 years old and normal nutritional status (59.1%). Patients generally had abdominal mass (97.7%) and most had intragonadal GCT (95.5%), with ovarium (76.2%) as the most common location. The most frequent GCT histopathology was yolk sac tumor (34.1%), with an Alpha-fetoprotein (AFP) level of >100.000 (6.8%). Almost all patients (97.7%) had undergone surgery; however, 47% of patients did not complete their treatment. At the end of treatment, the majority of patients survived (86.4%).Conclusion: Germ cell tumor has various clinical characteristics. Understanding these characteristics will enable clinicians to make a proper diagnosis and provide immediate management that will lead to a better prognosis
Anti-migration Effect of Aaptos suberitoides Fraction in HCT-116 Colorectal Cancer Cell Line
Get PDFBackground: Colorectal cancer is the second leading cause of mortality and the most prevalent cancer worldwide. Most patients, who come with late-stage, have ineffective treatments and some side effects in chemotherapy. Aaptos suberitoides has potential anti-cancer effects due to its bioactive compounds such as aptamine. This study aimed to evaluate the migration inhibition effect of Aaptos suberitoides fraction in HCT-116 cell line.Methods: This study was an experimental study. Aaptos suberitoides specimen was taken in Tinjil Island and fractionated with ethyl acetate. HCT-116 cell line was added with Aaptos suberitoides fraction and cellular migration activity was observed in 48 hours of which the scratch assay was performed. The gap closure area was determined with ImageJ software.Results: The data showed that a low concentration of Aaptos suberitoides fraction inhibited migration activity in HCT-116 cell line as follow; 1 and 5 mg/L Aaptos suberitoides fraction inhibit 3-4 % cancer cell migration in 24 hours, and 10-11% inhibition in 48 hours, respectively. However, 10 mg/L fraction concentration only inhibited 7-14% of the migration effect.Conclusion: Aaptos suberitoides fraction suggests insignificant migration inhibition in colorectal cancer cells and only inhibits less than 15 % HCT-116 cell line
Lethal Dose of Calcium Bentonite in Wistar Rats
Get PDFBackground: Calcium bentonite is a type of clay produced by a devitrification of volcanic ash which is often used as a traditional medicine to absorb toxins and waste products of metabolism. The aim of this study was to determine the lethal dose of calcium bentonite in Wistar rats to explore its toxicity level and safe use.Methods: Fifty male and female Wistar rats were randomly divided into five groups for each sex. The control group was given 5 cc aquadest whereas the other four groups received calcium bentonite solution of 50 mg/kgBW, 300 mg/kgBW, 2,000 mg/kgBW, and 5,000 mg/kgBW, respectively, at a single dose. The weight was observed for up to 7 days and analyzed using the unpaired t-test and Mann-Whitney test. The death rate was calculated using the probit analysis. Median lethal dose results were then classified according to Loomis Criteria. Results: No deaths occurred at the highest dose, suggesting that the median lethal dose value of calcium bentonite was >5,000 mg/kgBW. No weight loss occurred due to the administration of calcium bentonite and a significant increase in the body weight was even observed in the male rat group. Interestingly, a significant decrease was found in the female rats group when compared to the control group.Conclusions: Calcium bentonite is classified as a practically non-toxic material with a median lethal dose of over 5,000 mg/kgBW
Implementation of Youth Information and Counseling Centers Related to HIV/AIDS Prevention in a Junior High School in Bandung
Get PDFBackground: Adolescents are the population group most at risk of HIV infection due to low knowledge about HIV/AIDS. The National Population and Family Planning Board (Badan Kependudukan dan Keluarga Berencana Nasional, BKKBN) has developed the Youth Information and Counseling Center (Pusat Informasi dan Konseling Remaja, PIK-R) Program as a forum for information and counseling services, one of which is about Adolescent Reproductive Health (ARH) including HIV/AIDS. This study aimed to explore whether the implementation of the programs related to HIV/AIDS prevention in adolescents, specifically in junior high school in Bandung City.Methods: A qualitative descriptive study was carried out at a junior high school in Bandung in August 2018. This study used a purposive sampling technique and data were collected by conducting in-depth interviews with nine program administrators who were then transcribed and coded based on the PIK-R management guidelines using the NVIVO12 software, observing the program activities and documentations study which were then triangulated.Results: Several indicators in the growing stage had been implemented according to the guidelines, consisting of mastering ARH education materials including HIV/AIDS, conducting activities in the form of providing materials and peer counseling although without a secretariat room, and establishing networks and partnerships.Conclusion: The PIK-R program can be a media for preventing HIV/AIDS among junior high school students in Bandung regardless of resource limitations. Therefore, supports from BKKBN and the school itself is needed to improve the implementation of this program
Profile of Osteomyelitis Patients Visiting the Orthopedic Clinic of Dr. Hasan Sadikin General Hospital, Indonesia, in 2017–2018
Get PDFBackground: Osteomyelitis is an inflammatory process caused by microorganism infection that leads to bone destruction. Osteomyelitis may affect all bones, particularly long bones, and infects all ages. This disease is hard to diagnose and the treatment is complex due to the disease’s heterogenicity, pathophysiology, clinical presentation, and management. This study aimed to determine the profile of osteomyelitis inpatients and outpatients presented to the Orthopedic Clinic of Dr. Hasan Sadikin General Hospital Bandung, IndonesiaMethods: This was a descriptive cross-sectional study conducted from July to October 2019 using the total sampling method. Data were collected from the medical records of osteomyelitis inpatients and outpatients presented to the Orthopedic Clinic of Dr. Hasan Sadikin General Hospital Bandung, Indonesia from 2017–2018. Data were then analyzed descriptively and the results were presented in frequencies.Result: In total, 90 data were retrieved. Most of data presented male patients (n= 69, 76.7%) with the age range of 20–29 years old (24.4%). The majority of these patients experienced chronic onset of disease (94.4%) located on the tibia (51.1%), which was caused by a post-operative procedure (61.1%). The most common treatment was operative procedure without antibiotic beads (51.1%). Staphylococcus aureus was the common pathogens identified in these patients (22.2%).Conclusion: The majority of osteomyelitis patients are males in productive age with chronic onset of disease located on the tibia caused by a post-operative procedure. Staphylococcus aureus is the common pathogen involved and the most common treatment is an operative procedure without antibiotic beads
Patient Satisfaction on Surgical Service Decision Time in Emergency Department of A Tertiary Hospital in Indonesia
Get PDFBackground: Patient satisfaction survey is essential for evaluating and developing plans to improve health services' quality. One of the emergency department service quality indicators is decision time. Overcrowding of the emergency room as a result of long decision time may cause dissatisfaction among patients. This study aimed to evaluate the satisfaction level of patients receiving surgical services and the correlation between the satisfaction level and surgical service decision time.Methods: A cross-sectional descriptive analytical study was conducted from September to November 2019 at the emergency department of Dr. Hasan Sadikin General Hospital. The consecutive sampling method was applied and a total of 110 patients met the inclusion and exclusion criteria. Patient satisfaction was assessed using a validated questionnaire and data on decision time was obtained from the patient’s medical record. Spearman correlation test was used to analyze the data.Results: This study discovered that most of the respondents were satisfied with the service (92.7%) and that decision was mostly made in less than 2 hours (79.1%). A weak negative correlation (ρ = - 0.144), was identified between decision time and patient satisfaction, albeit insignificant (p = 0.067), with a confidence interval of 95% (α = 5%).Conclusion: This study suggested that there is no correlation between patient satisfaction and decision time. Thus, decision time is not the main factor that determines patient satisfaction