Coordenadoria Geral da Universidade
Repositorio da Producao Cientifica e Intelectual da UnicampNot a member yet
169449 research outputs found
Sort by
The cytotoxic effect of RNA-guided endonuclease cas9 on human hematopoietic stem and progenitor cells (HSPCs)
No full textSem informação241S225S22
Chrysobalanus icaco L. Anthocyanins reduced cell proliferation and inflammation in HT‐29 colon cancer cells
No full textSem informação291CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQSem informaçã
Species distribution and introgressive hybridization of two avicennia species from the western hemisphere unveiled by phylogeographic patterns
Full text linkMangrove plants grow in the intertidal zone in tropical and subtropical regions worldwide. The global latitudinal distribution of the mangrove is mainly influenced by climatic and oceanographic features. Because of current climate changes, poleward range expansions have been reported for the major biogeographic regions of mangrove forests in the Western and Eastern Hemispheres. There is evidence that mangrove forests also responded similarly after the last glaciation by expanding their ranges. In this context, the use of genetic tools is an informative approach for understanding how historical processes and factors impact the distribution of mangrove species. We investigated the phylogeographic patterns of two Avicennia species, A. germinans and A. schaueriana, from the Western Hemisphere using nuclear and chloroplast DNA markers. Our results indicate that, although Avicennia bicolor, A. germinans and A. schaueriana are independent lineages, hybridization between A. schaueriana and A. germinans is a relevant evolutionary process. Our findings also reinforce the role of long-distance dispersal in widespread mangrove species such as A. germinans, for which we observed signs of transatlantic dispersal, a process that has, most likely, contributed to the breadth of the distribution of A. germinans. However, along the southern coast of South America, A. schaueriana is the only representative of the genus. The distribution patterns of A. germinans and A. schaueriana are explained by their different responses to past climate changes and by the unequal historical effectiveness of relative gene flow by propagules and pollen. We observed that A. bicolor, A. germinans and A. schaueriana are three evolutionary lineages that present historical and ongoing hybridization on the American continent. We also inferred a new evidence of transatlantic dispersal for A. germinans, which may have contributed to its widespread distribution. Despite the generally wider distribution of A. germinans, only A. schaueriana is found in southern South America, which may be explained by the different demographic histories of these two species and the larger proportion of gene flow produced by propagules rather than pollen in A. schaueriana. These results highlight that these species responded in different ways to past events, indicating that such differences may also occur in the currently changing world.15CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPSem informação2007/57021-9; 2010/50033-4; 2013/08086-
Women’s values and preferences for thromboprophylaxis during pregnancy: a comparison of direct-choice and decision analysis using patient specific utilities
No full textWomen with a history of venous thromboembolism (VTE) have an increased recurrence risk during pregnancy. Low molecular weight heparin (LMWH) reduces this risk, but is costly, burdensome, and may increase risk of bleeding. The decision to start thromboprophylaxis during pregnancy is sensitive to women’s values and preferences. Our objective was to compare women’s choices using a holistic approach in which they were presented all of the relevant information (direct-choice) versus a personalized decision analysis in which a mathematical model incorporated their preferences and VTE risk to make a treatment recommendation. Multicenter, international study. Structured interviews were on women with a history of VTE who were pregnant, planning, or considering pregnancy. Women indicated their willingness to receive thromboprophylaxis based on scenarios using personalized estimates of VTE recurrence and bleeding risks. We also obtained women’s values for health outcomes using a visual analog scale. We performed individualized decision analyses for each participant and compared model recommendations to decisions made when presented with the direct-choice exercise. Of the 123 women in the study, the decision model recommended LMWH for 51 women and recommended against LMWH for 72 women. 12% (6/51) of women for whom the decision model recommended thromboprophylaxis chose not to take LMWH; 72% (52/72) of women for whom the decision model recommended against thromboprophylaxis chose LMWH. We observed a high degree of discordance between decisions in the direct-choice exercise and decision model recommendations. Although which approach best captures individuals’ true values remains uncertain, personalized decision support tools presenting results based on personalized risks and values may improve decision making.136234134
Pregnancy rates in couples with recurrent implantation failure in in vitro fertilization subjected to paternal leukocyte immunization
No full textsem informação3645125126th Latin American Symposium on Maternal-Fetal Interaction and Placenta / 5th Latin American Symposium on Reproductive Immunolog
Are there bandits at serra do mar state park? Conflicts, strategies and multiple uses of natural resources in the Atlantic Forest of São Paulo
Full text linkO histórico da relação entre os moradores e os gestores do Núcleo Picinguaba do Parque Estadual da Serra do Mar (PESM), localizado no litoral norte do Estado de São Paulo, está caracterizado pelos conflitos relacionados aos direitos de permanência e de uso dos recursos naturais. Criado em 1977, o PESM permaneceu no papel até inícios dos anos 1980, quando começaram os primeiros contatos com os moradores. Estes moradores, trabalhadores do campo e pescadores, viram, então, deslegitimados seus direitos ao trabalho e à continuação de suas atividades produtivas e culturais. Desde então, passaram a ser considerados clandestinos, ilegais ou, segundo suas próprias palavras, “bandidos”, devido a que a legislação proíbe a presença permanente de moradores neste tipo de áreas. O objetivo desta pesquisa foi analisar os conflitos, as estratégias e a organização dos diversos atores sociais vinculados ao Núcleo Picinguaba a respeito do uso dos recursos naturais e do acesso à terra e, finalmente, refletir se esta dinâmica influencia positivamente nos processos sociais associados à conservação da biodiversidade. Este conflito originou novas formas de organização no PESM: lideranças locais surgiram e se formaram associações comunitárias. Ao longo dos últimos anos, o diálogo entre gestão e moradores tem se intensificado e novos espaços de discussão e negociação, assim como novos atores com seus próprios interesses, têm aparecido. Três questões são transversais a este conflito: a questão da terra, a questão da identidade e a própria questão do uso e da conservação dos recursos naturais. Assim, os moradores têm se organizado em torno da luta pelo direito à terra, recorrendo para isso a estratégias identitárias e a categorias como populações tradicionais, quilombolas e caiçaras. Tudo isto em um contexto de uma UC de Proteção Integral, localizada em uma região não só altamente biodiversa, mas com um forte histórico de uso dos recursos naturais e de ocupação humana387799The history of the relationship between inhabitants and managers of the Picinguaba Administrative Nucleus in the Serra do Mar State Park (SMSP) is characterized by conflicts related to the rights to permanence and to the use of natural resources. The SMPS is located in the northern coast of São Paulo State, the richest and more developed state of Brazil. Created in 1977, the park remained on paper until the beginning of the 1980s decade. At this moment, these inhabitants, rural workers and fishermen, were delegitimized and lost their rights to work and to continue their cultural and productive activities. Since then, the inhabitants were considered illegal or, on their own words, “bandits”, due to the Brazilian law prohibiting the presence of inhabitants inside the Protected Areas with strictly protection. This research aims to analyze the conflicts, strategies and organization of the different social actors (inhabitants, managers, members of NGOs and researchers) related to the Picinguaba Nucleus, the use of natural resources and the access to land. All these actors have their own interests, perspectives, struggles and action strategies, and organize themselves around them in a very complex and multi-level arena. This conflict caused new forms of social organization in the SMSP: local leaders arose and communal associations that aim the struggle for the inhabitants rights were created. Three issues are transversal to this conflict and the strategies followed by the actors along the years: land issue, identity issue and use and conservation of natural resources issue. Thus, the inhabitants were organized around the struggle for their right to land, and using categories such as traditional people. All of this in a context with a Protected Area with strictly protection, located in a region not only with high biodiversity, but with a history of use of natural resources and human occupatio
Fatigue crack growth rate of two pearlitic rail steels
No full textA study of fatigue crack growth rate was conducted in two pearlitic rail steels, namely R260 and R370CrHT. Two crack plane orientations with respect to the rolling direction were tested and separate experiments were performed to cover a wide variation in fatigue crack growth, from the fatigue threshold (circa 10 (9) m/cycle) to rapid fracture conditions. The fracture surfaces were examined and some correlations between microstructural features such as interlamellar spacing and mechanical properties were made1386372Brazilian Nanotechnology National Laboratory (LNNano, Campinas); Medellin City Hall with his program "Enlazamundos"; Departamento Administrativo de Ciencia, Tecnologia e Innovacion Colciencias; Metro system of Medellin-Colombi
Evans syndrome at childhood‐onset systemic lupus erythematosus diagnosis: a large multicenter study
No full textEvans syndrome (ES) in childhood‐onset systemic lupus erythematosus (cSLE) patients has been rarely reported and limited to small populations. A retrospective multicenter cohort study (Brazilian cSLE group) was performed in 10 Pediatric Rheumatology services including 850 patients with cSLE. ES was assessed at disease diagnosis and defined by the combination of immune thrombocytopenia and autoimmune hemolytic anemia. ES was observed in 11 of 850 (1.3%) cSLE patients. The majority of them had hemorrhagic manifestations (91%) and active disease (82%). All patients with ES were hospitalized and none died. Comparisons of cSLE patients with and without ES at diagnosis revealed similar frequencies of female gender, multiorgan involvement, autoantibodies profile, and low complement (P > 0.05). Patients with ES had a lower frequency of malar rash (9% vs. 53%, P = 0.003) and musculoskeletal involvement (18% vs. 69%, P = 0.001) than those without this complication. The frequencies of intravenous methylprednisolone (82% vs. 43%, P = 0.013) and intravenous immunoglobulin use (64% vs. 3%, P < 0.0001) were significantly higher in the ES group, with similar current prednisone dose between groups (1.1 [0.76–1.5] vs. 1.0 mg/kg/day [0–30], P = 0.195). Our large multicenter study identified ES as a rare and severe initial manifestation of active cSLE with good outcome. Diagnosis is challenging due to the lack of typical signs and symptoms of lupus and the requirement to exclude infection and primary immunodeficiency.63712381243CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ303422/2015‐
Curli fimbria: an Escherichia coli adhesin associated with human cystitis
Full text linkEscherichia coli is the major causative agent of human cystitis. In this study, a preliminary molecular analysis carried out by PCR (polymerase chain reaction) demonstrated that 100% of 31 E. coli strains isolated from patients with recurrent UTIs (urinary tract infections) showed the presence of the curli fimbria gene (csgA). Curli fimbria is known to be associated with bacterial biofilm formation but not with the adhesion of human cystitis-associated E. coli. Therefore, this work aimed to study how curli fimbria is associated with uropathogenic E. coli (UPEC) as an adhesion factor. For this purpose, the csgA gene was deleted from strain UPEC-4, which carries three adhesion factor genes (csgA, fimH and ompA). The wild-type UPEC-4 strain and its mutant (ΔcsgA) were analyzed for their adhesion ability over HTB-9 (human bladder carcinoma), Vero (kidney cells of African green monkey) and HUVEC (human umbilical vein) cells in the presence of α-D-mannose. All the wild-type UPEC strains tested (100%) were able to adhere to all three cell types, while the UPEC-4 ΔcsgA mutant lost its adherence to HTB-9 but continued to adhere to the HUVEC and Vero cells. The results suggest that curli fimbria has an important role in the adhesion processes associated with human UPEC-induced cystitis.472414416CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQSem informaçã