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Production and purification of extreme xylanase from Aspergillus flavus AUMC 10331 in sub-merged fermentation
Get PDFXylan, extracted from oat spelts in a previous work, was assayed by HPLC and used as carbon source for the production of xylanase from Aspergillus flavus AUMC 10331. The produced xylanase was purified using ion exchange resin (IR-120 EP) and gel filtration column of Sephadex G-75 and Sephadex G-100. The purified xylanase showed total activity of 5.5 IU/ml and specific activity of 687.5 IU/mg, and the enzyme purified 156.75 fold with 4.43 % yield. The highest activity at pH 7.0 and 10.5 indicating two xylanases with the most interesting one with a maximum activity at pH 10.5 and 65 °C. The enzyme activity was greatly stimulated by 5 mM of FeSO4 and CuSO4, while slightly inhibited by other metal ions. Km and Vmax were determined as 8.36 mg/ml and 172.4 IU/min respectively.
DOI: http://dx.doi.org/10.5281/zenodo.258610
Phenolics content, antiproliferative and antioxidant activities of Algerian Malva sylvestris
Get PDFDue to its expected low toxicity to human use, more attention is given worldwide to antioxidants of natural sources. Therefore, the extraction of the total phenolic compounds contained in the leaves of Malva sylvestris and the analysis of the polyphenols, flavonoids and tannins contents were carried out. The antioxidant activity of the hydro-methanolic extract of Malva sylvestris was investigated employing various established in vitro systems including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, the reduction of hydrogen peroxide and the ferric reducing power assay. The antiproliferative activity of plant extract was tested against three tumor cell lines: MCF-7, Hep2 and WEHI using 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphynyl tetrazolium bromide (MTT) assay. Preliminary screening indicated the presence of substances with large therapeutic values: an important content of polyphenols, flavonoids and tannins was detected in the tested extract. Our data showed that the extract exhibited high antioxidant properties, which were demonstrated by its ability to scavenge 76.11% of DPPH free radicals, and the elimination of 69.97% of hydrogen peroxide at a concentration of 125 µg/ml. In addition, the plant extract showed strong ferric reducing power which was a function of the sample concentration. For the antiproliferative activity, the results demonstrated that the plant extract significantly inhibited tumor cell growth and colony formation in a concentration-dependent manner. The toxicity percentage of extract at 125 µg/ml on MCF-7, Hep2 and WEHI was found in the order of 45.20%, 62.62% and 82.04%, respectively.
DOI: http://dx.doi.org/10.5281/zenodo.254591
Therapeutic effect of fractionated by ultrafiltration red beetroot (Beta vulgaris L.) juice in rats with food-induced fatty liver
Get PDFThe prevalence of non-alcoholic fatty liver disease (NAFLD), being a component of metabolic syndrome, has increased (15-27%) in the industrialized world. The deep mechanism of this pathology is not clear, but it is multifactorial. There is a huge amount of food supplements and medicines with hepatoprotective effect on the market, but the NAFLD problem is far from being resolved. Hepatoprotective products have to provide wide spectra of biological effects, including antioxidant, hypolipidemic, anti-inflammatory action. It is peculiar to natural compounds, including red beetroot juice, which is well known to most of the population. This is important in view of the high prevalence of NAFLD. The aim of this study is to evaluate the curative effect of fractionated by ultrafiltration red beetroot juice in rats with food-induced liver steatosis.
DOI: http://dx.doi.org/10.5281/zenodo.254107
Biosynthesis of silver nanoparticle from fungi, algae and bacteria
Get PDFSilver nanoparticles are today considered as the backbone of nanotechnology industries. Since time immemorial silver along with its compound and associated salts have been walking together with human civilization. Although the silver has been known from such a long time it has not been recently that fabrication of silver nanoparticle was to be a reality. It has some prominent as well as pronounced application in the field of medicine, agriculture etc. It has very favorable and significant antioxidant, antibacterial and antifungal properties. It has been found effective against many of bacteria’s such as Vibrio parahaemolyticus, Citrobacter koseri, Salmonella Typhii, Pseudomonas aeruginosa, Staphylococcus aureus and even against few fungus species like Candida albicans. The mode of mechanism could be possible binding of silver ions with the biomolecules present in cells. It is believed that the whole system runs over the fact that it leads to the formation of free radical along with the production of ROS i.e. reactive oxygen species, which ultimately result in apoptotic condition and hence cell could no longer replicate. There is much more application ranging from food preservation, cosmetic etc. But the physical and chemical synthesis of Ag has been inefficient to meet the demands at the same time causing lots of damage to the environment. Hence it calls for a cleaner, efficient and eco-friendly process. That space has been traveled by biosynthesis of Ag nanoparticle from plant, algae, and bacteria etc. This review takes under consideration such efforts in the last few years.
DOI: http://dx.doi.org/10.5281/zenodo.261716
Antifungal activity of the rhizome extracts of Pulsatilla vulgaris against Candida glabrata
Get PDFPulsatilla vulgaris Mill. (“Pasque flower”, Ranunculaceae) is rare and a threatened plant species in Europe. It produces biologically active secondary metabolites. P. vulgaris is also known herbal drug used for centuries in traditional Chinese and Korean medicine. The rhizomes of P. vulgaris have been traditionally used for treatment of headaches, neuralgia, insomnia, hyperactivity, bacterial skin infections, septicemia, cough and bronchitis. In the present study, the extracts of leaves and rhizomes of P. vulgaris were evaluated for their antifungal, antimicrobial, antimalarial and cytotoxic activities. The results showed the antifungal activity of crude extracts of the rhizome of P. vulgaris against the yeast Candida glabrata with an IC50 of 11 µg/ml. These results indicate that the selected medicinal plant could be further investigated for identifying compounds that may be responsible for the observed activity and that may represent new leads in fungal drug discovery.
DOI: http://dx.doi.org/10.5281/zenodo.322889
Lassa fever and the Nigerian experience: a review
Get PDFThe occurrence, transmission and intervention strategies on the Lassa fever disease in Nigeria are presented. The Lassa virus is an enveloped, single stranded, bi-segmented RNA virus that belong to the Arenaviridae family was first reported in 1969 from Lassa village, Borno State, Nigeria. The primary animal reservoir for the virus is the multi-mammate rat (Mastomys natalensis). It is transmitted to humans through the excreta of infected carrier, often via contaminated food and human-to-human transmission. The most common treatment intervention is ribavirin which carries out its function by inhibiting virus replication. Extensive investigation is being carried out to arrive at an effective vaccine. Keeping rodents out of homes and food supplies, as well as maintaining effective personal hygiene are the most viable preventive measures against the disease.
DOI: http://dx.doi.org/10.5281/zenodo.338269
Assessment of bacteriological quality of drinking water in Belagavi City, India
Get PDFWith the growing population and industrialization, the potability of drinking water has been decreased due to pollution and improper sanitization. In this context the present study was aimed to check the potability of drinking water by Multiple Tube method and Membrane Filter technique and to identify bacteria from Membrane Filter technique. 100 samples from 10 different wards of Belagavi City were collected. Bacteriological analysis was done for the presence of fecal coliforms, fecal Streptococci, Salmonella and Shigella by presumptive coliform test and multiple tube method (MPN) and membrane filter (MF) techniques were used for estimation of coliform bacteria. Twelve out of 100 samples were found to be unsatisfactory for drinking purpose. A total of five different strains were isolated from 100 samples. Genera isolated were Corynebacterium species, Micrococci, Gram positive Bacilli, Staphylococcus aureus, Citrobacter freundii. 88% of the sources were hygienic for drinking purposes and for the rest of the sources, proper measure should be taken to maintain sanitary of the drinking water by regular check on the equipment, maintaining structural faults and proper disinfection of equipments.
DOI: http://dx.doi.org/10.5281/zenodo.279615
Removal of hydrocarbons from crude oil contaminated agricultural soil by phytoremediation using Mariscus alternifolius and Fimbristylis ferruginea
Get PDFCrude oil extraction is one major route through which hydrocarbons are released into the environment and hydrocarbon contamination is highly hazardous to the ecosystem. This study investigated the removal of hydrocarbons from crude oil contaminated agricultural soils using Mariscus alternifolius Vahl. and Fimbristylis ferruginea plant species. Before planting, the polluted soil (negative control) had a total petroleum hydrocarbon concentration of 17962.11±1000.00 mg/kg and polycyclic aromatic hydrocarbon concentration of 440.97±1.00 mg/kg. Likewise, the soil oil and organic carbon contents were 3.25±0.10 ppm and 3.06±0.02% respectively. Results, 90 days after planting, indicated a significant decrease in the total petroleum hydrocarbon concentrations of M. alternifolius (100.82±46.31 mg/kg) and F. ferruginea (110.41±39.68 mg/kg) treated soils. Likewise, there was a significant decrease in the polycyclic aromatic hydrocarbon concentration of M. alternifolius treated soil (95.69±65.44 mg/kg). The oil content of the treated soils significantly decreased to 1.03±0.28 ppm and 0.84±0.33 ppm in M. alternifolius and F. ferruginea treated soils respectively, while the organic content of the treated soils significantly decreased to 2.16±0.09% and 2.20±0.20% in M. alternifolius and F. ferruginea treated soils respectively. Phytoremediation using M. alternifolius and F. ferruginea has proven to be potent in the remediation of hydrocarbon contaminated soil through enhancement and recovery of the polluted soil. These plant species which improved the cultivation and germination competence of the treated soils thus making the soil probable for agricultural and other related purposes are therefore recommended for used in the phytoremediation of crude oil contaminated soils.
DOI: http://dx.doi.org/10.5281/zenodo.260391
Efficacy of octenidine against Pseudomonas aeruginosa strains
Get PDFPseudomonas aeruginosa is a Gram-negative bacterium causing skin and soft tissue infections, complicated urinary tract infections, blood infections, and nosocomial (hospital-acquired) infections. One of the most often used antiseptics in the skin and soft tissue infections is octenidine dihydrochloride. The aim of this study was an evaluation of octenidine activity against strains of P. aeruginosa. Additionally, were compared two staining methods (TTC and MTT) for confirmation of bacterial growth. The study involved eight strains of P. aeruginosa. In order to determine the minimum inhibitory concentration (MIC) of octenidine, the microdilution method was used. For bacterial growth detection was used staining method with 2,3,5-triphenyl-tetrazolium chloride (TTC) and with 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT). In the study has been demonstrated the excellent activity of octenidine against all strains of Pseudomonas aeruginosa. For all tested strains, MICs of octenidine were 0.00039% or 0.00078%, what is equivalent to 3.9 µg/ml and 7.8 µg/ml, respectively. In the study, test with MTT for three strains was more sensitive than a test with TTC. Concluding, octenidine is an antiseptic with high efficacy against Pseudomonas aeruginosa strains. Simultaneously, it was stated that a test with MTT is more sensitive than study with TTC.
DOI: http://dx.doi.org/10.5281/zenodo.333949
Microbial-aided phytoremediation of heavy metals contaminated soil: a review
Get PDFAnthropogenic exercises as well as industrial enterprise and agricultural practices contribute considerably to the degradation and contamination of environment that considerably affects the soil. The normal physical and chemical know-how soil washing used for soil remediation render the land useless as a medium for plant growth, as they take away all biological activities. Others are labor-intensive and have high maintenance value phytoremediation, a cheaper and sustainable in situ remediation technique was so thought of. This data can enable proposing solutions to issues of contamination and eventually convalescent sites and soils. However, plants don't have the aptitude to degrade several soil waste matters particularly the organic pollutant. It's so imperative to require advantage of the degrading ability of soil microorganisms. This review so focuses on phytoremediation techniques improved by microbial colonies.
DOI: http://dx.doi.org/10.5281/zenodo.324417