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بررسی رابطه سرمایه فکری با عملکرد نوآورانه با نقش میانجی توانمند سازی کارکنان حوزه بهداشت دانشگاه علوم پزشکی قزوین-1399
Full text linkAnticancer Properties of Probiotic Saccharomyces boulardii Supernatant on Human Breast Cancer Cells
Full text linkSaccharomyces boulardii, a variety of S. cerevisiae, is used as a probiotic yeast in food and drug industries. However, S.
boulardii is an opportunistic pathogen, and the supernatant of this organism has recently been recommended for its healthpromoting benefts. Breast cancer is the most frequent cancer disease in women worldwide. The objective of this study was to
investigate the efects of S. boulardii supernatant (SBS) on cell viability, inducing apoptosis and suppression of survivin gene
expression in MCF-7 and MCF-7/MX as human non-drug-resistant and multidrug-resistant breast cancer cells respectively.
The IC50 value of SBS against MCF-7 was calculated 1037, 542, and 543 µg/mL for 24, 48, and 72 h treatments, respectively.
Also, this value against MCF-7/MX cells were measured 1242, 616, and 444 µg/mL after 24, 48, and 72 h respectively.
We found that suppression of survivin gene expression should be one of the main molecular antitumor mechanisms which
is contributed to apoptosis in breast cancer cells. However, anticancer activity of SBS was observed more efcient against
MCF-7 than that against MCF-7/MX cells. SBS is suggested to be considered as one of the prospective anticancer drugs to
treat human breast carcinoma. More investigations especially in vivo studies are strongly recommended to be implemented
to characterize other antitumor mechanisms of SBS against breast carcinoma.
Keywords Anticancer properties · Breast carcinoma · In vitro study · Probiotic Saccharomyces boulardii · Yeast
supernatan
Expression of virulence factor genes in co-infections with Trueperella pyogenes isolates and other bacterial pathogens; an in vivo study
Full text linkTrueperella pyogenes is an opportunistic bacterial pathogen causing several infectious diseases, including metritis,
mastitis and abscesses in domestic animals such as dairy cattle. Several virulence proteins are released by
T. pyogenes strains contributing to the pathogenic and causing disease potential of this pathogen. So far, many
aspects of T. pyogenes pathogenesis are unknown. In this study, expression levels of plo, fimA, nanH and cbpA
genes encoding pyolysin, fimbriae, neuraminidase and collagen-binding protein, respectively in T. pyogenes
isolated from totally 15 metritis, mastitis and cutaneous abscesses convenience samples in response to co-culture
with other pathogens including E. coli, St. dysgalactiae, S. aureus, F. necrophorum and L. plantarum strains in mice
study model have been investigated. We found that expression levels of plo, fimA, nanH and cbpA genes in
T. pyogenes isolates in response to co-culture with F. necrophorum and E. coli were significantly increased;
however, no significant changes was seen in the level of expression of these genes in the isolates in response to
co-culture with St. dysgalactiae and S. aureus. Notably, expression of all virulence factor genes was suppressed in
T. pyogenes in response to co-culture with L. plantarum. We observed that L. plantarum might be used to prevent
infectious diseases caused by T. pyogenes
LncRNA–miRNA–mRNA Networks of Gastrointestinal Cancers Representing Common and Specific LncRNAs and mRNAs
Full text linkGastrointestinal (GI) cancers are responsible for approximately half of cancer-related
deaths, highlighting the need for the identification of distinct and common features in
their clinicopathological characteristics. Long ncRNA (lncRNAs), which are involved in
competitive endogenous RNA (ceRNA) networks with critical roles in biological processes,
constitute a substantial number of non-coding RNAs. Therefore, our study aimed to
investigate the similarities and differences in the ceRNA networks of The Cancer Genome
Atlas (TCGA)-GI cancers. We performed a comprehensive bioinformatics analysis of
ceRNA networks for TCGA-GI cancers in terms of the deferential mRNA, lncRNA, and
miRNA expression levels, ceRNA networks, overall survival analysis, correlation analysis,
pathological cancer stages, and gene set enrichment analysis. Our study revealed several
common and distinct mRNAs and lncRNAs with prognostic values in these networks. It
was specifically noteworthy that MAGI2-AS3 lncRNA was found to be shared in almost all
GI cancers. Moreover, the most common shared mRNAs between GI cancers were
MEIS1, PPP1R3C, ADAMTSL3, RIPOR2, and MYLK. For each cancer ceRNA network, we
found that the expression level of a number of lncRNAs and mRNAs was specific.
Furthermore, our study provided compelling evidence that several genes, most notably
KDELC1, can act as novel proto-oncogenes in cancers. This, in turn, can highlight their role
as new prognostic and therapeutic targets. Moreover, we found cell cycle and extracellular
matrix structural constituent as the top shared KEGG and molecular function, respectively,
among GI cancers. Our study revealed several known lncRNAs and known and unknown
mRNAs in GI cancers with diagnostic and prognostic value
Clinicopathological Significance of PTEN Expression and Its Prognostic Effect in Colorectal Adenocarcinoma Patients
Full text linkBackground & Objective: Phosphatase and tensin homolog (PTEN) is a tumor
suppressor gene located at chromosome 10. PTEN is a regulator of the PI3K/AKT
signaling pathway that inhibits cell proliferation and promotes apoptosis. PTEN loss of
function occurs in a spectrum of cancers, including colorectal adenocarcinoma. This
study aimed to investigate the probable correlation of negative PTEN expression with
clinicopathological features and colorectal adenocarcinoma (CRC) patients' survival.
Methods: In this cross-sectional study using Immunohistochemistry stainingPTEN
expression status on 151 CRC tissues was evaluated. Then the results of IHC staining
was compared to those of clinicopathological features. The relationship between PTEN
and KRAS mutation status was also investigated.
Results: Of 151 CRC samples, 89 (58.9%) were negative for PTEN expression. Loss
of PTEN expression was associated with KRAS mutation (P<0.0001), lymph node
metastasis (P=0.002), and advanced tumor stage (P=0.016), whereas no significant
association was found with other clinicopathological features. Multivariate analysis
indicated that tumor site and KRAS mutation were independent prognostic CRC
patients (P<0.05). The Kaplan-Meier analysis indicated a correlation between loss of
PTEN expression and overall survival of patients with colorectal adenocarcinoma (P=
0.01).
Conclusion: The current study suggests that decreasing PTEN expression or its negative
expression may be associated with a higher stage and poor prognosis. Combined analysis of
mutated KRAS and PTEN expression could be a good predictor of disease prognosis as well
as its clinical outcome
The effect of dynamic loading on bacterial microleakage of the dental implant fixture-abutment interface: An in vitro study
Full text linkEvaluation of Pregnancy consequences in Women with Polycystic Ovary Syndrome: A Case-Control Study in Qazvin, Iran
Full text linkBackground and Aim: Polycystic ovary syndrome (PCOS) is a common disorder in women
of childbearing age that can be associated with adverse pregnancy complications. The aim of
this study was to compare the adverse pregnancy consequences between pregnant women
with PCOS and control group in Qazvin.
Materials and Methods: In this study, 150 pregnant women with PCOS aged 18-35 years
with gestational age of more than 20 weeks were selected as our case group based on
Rotterdam criteria and our control group included 150 pregnant women without PCOS. The
groups were compared in terms of pregnancy complications including gestational diabetes,
preeclampsia, preterm delivery, low birth weight, and neonatal admission to the neonatal
intensive care unit (NICU).
Results: There were no significant differences between the two groups in terms of
demographic data including age, weight, height and BMI. In the case group (PCOS) we found
gestational diabetes in 33 cases (64.7%) (P=0.021), preeclampsia in 44 cases (67.7%)
(P=0.002), and preterm delivery 70 in cases (75.3%) which indicated a significant difference
between the case and control groups (P<0.0001). The case group had higher neonatal
complications than control group in regard to low birth weight (40 cases, 64.5%, P=0.008)
and NICU admission (30 cases, 66.7%, P=0.015).
Conclusion: The results of this study showed that PCOS, due to its metabolic effects, may
increase the incidence of gestational diabetes, preeclampsia, preterm delivery, low birth
weight or macrosomia and long term NICU admission.
Keywords: Polycystic ovary syndrome, Premature birth, Gestational diabetes, Pre-eclampsi
Molecular identification and phylogenetic analysis of free-living amoeba in the water resources of Arak, Iran
Full text linkThe aim of the present study was to detect free-living amoeba (FLA) in the water resources of Arak, Iran using molecular tools. A total of 154
samples were collected from different water supplies. Molecular analyses, sequencing, and phylogenetic study were conducted to confirm
the species and genotypes of FLA. Fisher exact test was used to determine the significance. Of 154 water samples, 19 (12.3%) samples were
tested positive for FLA. Three genotypes of Acanthamoeba including T4, subtype D, and T5 were identified among the isolates. The pathogenicity assay showed that the isolate of Acanthamoeba in drinking water was highly pathogenic. Three species of Naegleria, including
N. australiensis, N. pagei, and N. gruberi were found among the samples. Six isolates of Vermamoeba were identified as V. vermiformis.
Meanwhile, three other species including Vannella sp., Vahlkampfia avara, and Stenamoeba polymorpha were also recovered from the
water samples. Statistical analysis showed a significant difference between the various water resources contaminated with FLA. This is
the first study to reveal the presence of S. polymorpha in water sources in Iran. According to the findings of the present study, health officials
should be beware of potential public health impacts of FLA in water resources
