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Long-Term Follow-up of Autologous Fibroblast Transplantation for Facial Contour Deformities, A Non-Randomized Phase IIa Clinical Trial
Get PDFObjective: Recently, the promising potential of fibroblast transplantation has become a novel modality for skin
rejuvenation. We investigated the long-term safety and efficacy of autologous fibroblast transplantation for participants
with mild to severe facial contour deformities.
Materials and Methods: In this open-label, single-arm phase IIa clinical trial, a total of 57 participants with wrinkles
(n=37, 132 treatment sites) or acne scars (n=20, 36 treatment sites) who had an evaluator’s assessment score of
at least 2 out 7 (based on a standard photo-guide scoring) received 3 injections of autologous cultured fibroblasts
administered at 4-6 week intervals. Efficacy evaluations were performed at 2, 6, 12, and 24 months after the final
injection based on evaluator and patient’s assessment scores.
Results: Our study showed a mean improvement of 2 scores in the wrinkle and acne scar treatment sites. At sixth
months after transplantation, 90.1% of the wrinkle sites and 86.1% of the acne scar sites showed at least a one grade
improvement on evaluator assessments. We also observed at least a 2-grade improvement in 56.1% of the wrinkle
sites and 63.9% of the acne scar sites. A total of 70.5% of wrinkle sites and 72.2% of acne scar sites were scored as
good or excellent on patient assessments. The efficacy outcomes remained stable up to 24-month. We did not observe
any serious adverse events during the study.
Conclusion: These results have shown that autologous fibroblast transplantation could be a promising remodeling
modality with long-term corrective ability and minimal adverse events (Registration Number: NCT01115634)
The emergence of quaternary ammonium compounds resistance in escherichia coli isolated from hospitals of qazvin, iran
Get PDFIntroduction: Escherichia coli is one of the most important agents involved in healthcareassociated infection, and resistance to quantum ammonium compounds (QACs) has become a
major challenge for infection control practitioners. The aim of the current study was to
determine the frequency of qacE and qacEΔ1 genes in E. coli isolated from hospitalized
patients in Qazvin, Iran. Material and Methods: In the current cross-sectional study, 102 E. coli
were collected from hospitals of Qazvin. All bacterial isolates were identified using standard
laboratory methods and the antimicrobial susceptibility was evaluated by Kirby-Baer test. The
presence of qacE and qacEΔ1 genes was investigated using polymerase chain reaction (PCR)
technique. Results: In this study, 65 (63.7%) isolates showed a multidrug resistance (MDR)
pattern which was resistant to at least three classes of antimicrobials including ß-lactams,
aminoglycosides, and fluoroquinolones. The highest rates of resistance were observed against
cefotaxime (75.5%) and nalidixic acid (66.7%). The PCR showed that 5 (4.9%) isolates
harbored qacE gene, 62 (60.8%) isolates qacEΔ1, and 10 (9.8%) isolates carried both genes,
simultaneously. There was a significant relationship between the QACs resistance and MDR
pattern (P=0.03). Conclusion: This study indicated a significant resistance rate against
disinfectant compounds in the studied hospitals. However, more attention should be paid to
this critical issue in the infection control committees of the hospital
Calprotectin (S100A8/S100A9)-induced cytotoxicity and apoptosis in human gastric cancer AGS cells: Alteration in expression levels of Bax, Bcl-2, and ERK2
Get PDFCalprotectin is a heterodimeric EF-hand Ca2+ binding protein that is typically released by
infiltrating polymorphonuclear leukocytes and macrophages. This protein is a key player
linking inflammation and cancer. Due to the increased levels of calprotectin in different
inflammatory diseases and cancer, it is considered as a marker for diagnostic purposes. In this
study, we evaluated the mechanism of cell viability and apoptotic-inducing effects of
recombinant human calprotectin (rhS100A8/S100A9) on the gastric adenocarcinoma (AGS),
the most common type of gastric cancer cell line. AGS cells were exposed to the different
concentrations (5–100 μg/ml) of calprotectin for 24, 48, and 72 h, and cell viability was
assessed through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
Apoptotic-inducing effects of calprotectin were evaluated by sub-G1 cell cycle assay and
Annexin V/propidium iodide double staining. Furthermore, real-time polymerase chain
reaction and Western blot analysis were performed to evaluate the mechanism of action of
calprotectin. Our findings indicated that calprotectin inhibits growth and viability of AGS cells
in a time- and dose-dependent manner. The half-maximal inhibitory concentration values were
measured as 85.77, 79.14, and 65.39 μg/ml for 24, 48, and 72 h, respectively. Additionally, we
found that calprotectin downregulated the expression of antiapoptotic protein Bcl-2 and
upregulated proapoptotic protein Bax in a time- and concentration-dependent fashion.
Calprotectin also slightly upregulated the expression of extracellular signal-regulated protein
kinase 2 (ERK2), while it significantly decreased the levels of phospho-ERK in a timedependent manner. Overall, these findings indicated that calprotectin has cytotoxicity and
apoptosis-inducing effects on AGS cell lines in high concentration by modulating Bax/Bcl-2
expression ratio accompanied by inhibition of ERK activation
Study of HSA interactions with arachidonic acid using spectroscopic methods revealing molecular dynamics of HSA-AA interactions
Get PDFThe interaction between human serum albumin
(HSA) and arachidonic acid (AA) as an unsaturated fatty
acid were investigated in the present study using methods
including UV-VIS spectrophotometry, fluorescence and
circular dichroism (CD) spectroscopy, lifetime measurements,
fluorescence anisotropy measurements and visual molecular
dynamics(MD). The thermodynamic parameters were assessed
from HSA thermal and chemical denaturation in the presence
and absence of AA. From the thermal denaturation, the Tm and
ΔG˚(298K) magnitudes obtained were 327.7 K and 88 kJ/mol,
respectively, for HSA alone, and 323.4 K and 85 kJ/mol, respectively, following treatment with a 10 µM AA concentration. The
same manner of reduction in Gibbs free energy as a criterion
of protein stability was achieved during chemical denaturation
by urea in the presence of AA. The present study investigates
HSA binding nature through MD approaches, and the results
indicated that the binding affinity of AA to the subdomain
IIA of HSA is greater compared with that of subdomain IIIA.
Although the HSA regular secondary structure evaluation by
CD exhibited a minor change following incubation with AA,
its tertiary structure revealed an observable fluctuation. Thus,
it appears that the interaction between AA and HSA requires
minor instability and partial structural changes
بررسی تاثیر مکمل یاری الاژیک اسید بر وضعیت آنتی اکسیدانی، فاکتورهای التهابی و کیفیت زندگی در بیماران دارای سندرم روده تحریک پذیر: مطالعه کارآزمایی بالینی دوسوکور تصادفی شده (فایل دفاع نهایی)
Get PDFComparison of the performance of fire risk assessment method for engineers (FRAME) and hospital safety index (HSI) in assessing and analyzing the fire risk level of high-risk wards of Velayat hospital in Qazvin in 2019
Get PDFThe effect of ginger and metoclopramide in the prevention of nausea and vomiting during and after surgery in cesarean section under spinal anesthesia
Get PDFObjective
Postoperative nausea and vomiting is one of the most common side effects after anesthesia in surgeries, such as
cesarean section. This study aimed to investigate the effect of ginger and metoclopramide in the prevention of
nausea and vomiting during and after cesarean section.
Methods
This clinical trial was conducted on 180 patients aged 18–40 years who underwent cesarean section under spinal
anesthesia. The first group received 10 mg of metoclopramide via intravenous injection (metoclopramide group), and
the second group received 1 g of oral ginger (ginger group) half an hour before spinal anesthesia. The frequency and
severity of nausea and vomiting during surgery and at 2, 6, 12, and 24 hours postoperatively were compared in both
groups. To analyze the results, the t-test, chi-square test, and Mann-Whitney test were used.
Results
There was no significant difference in the frequency of nausea and vomiting between the 2 groups during operation,
2 hours and 6 hours after surgery (P=0.182, 0.444 and 0.563 respectively). The severity of nausea and vomiting was
also similar in the 2 groups (P=0.487 and 0.652 respectively); however, the metoclopramide group had a lower systolic
blood pressure (P<0.001; df=2.176; f=18.66) and mean arterial pressure (P<0.001; df=2.176; f=6.36) than the ginger
group.
Conclusion
The results revealed that ginger reduced nausea and vomiting to the same extent as metoclopramide in patients
undergoing cesarean section
Molecular epidemiology of Blastocystis spp. in children referred to Qods hospital in northwest of Iran
Get PDFAbstract Blastocystis sp. is a polymorphic intestinal parasite in humans and animals. The parasite has a worldwide
distribution, especially in developing countries with poor
sanitation, exposure to animals, and improper disposal
systems. The aim of this study was to identify the subtypes
of Blastocystis sp. among children of Qazvin, northwest
Iran. Totally, 864 stool samples were collected from the
children referred to Qods hospital in Qazvin, Iran. Fecal
specimens were investigated by formalin-ethyl acetate
concentration method and trichrome staining as well as
cultivation of all samples in clotted fetal bovine medium.
DNA extraction of culture-positive specimens and PCR
amplification of 18S ribosomal RNA gene region was
performed. The sequences detected were compared with
reference genes in the GenBank, and the sequences further
deposited in the GenBank database. Data analysis was
performed by Chi square test while a p value of\0.05 was
considered as significant. Of 864 isolates, 4.1% (36/864)
were positive for Blastocystis sp. with infection rate
insignificantly higher among the females than males. The
highest infection rate was estimated at 6.8% in 6–9 years
old age group with abdominal pain as the most common
(33%) gastrointestinal sign. No statistically significant
difference was found between the variables and Blastocystis infection. Molecular analysis clarified the presence
of three subtypes of Blastocystis including ST1 (56%), ST2
(28%), and ST3 (16%) of among specimens with ST1 as
the predominant subtype. A significant association between
intestinal signs and the subtypes was not found. Considering ST1 as the predominant subtype, it seems that zoonotic transmission is a main route of human infections with
Blastocystis sp. in the area studied
Distribution and molecular analysis of Blastocystis subtypes from gastrointestinal symptomatic and asymptomatic patients in Iran
Get PDFIntroduction: Blastocystis is a common intestinal parasite of human and animal hosts. The parasite has 17 subtypes, and
among those at least nine subtypes (ST1-ST9) are found in human hosts.
Objective: The aim of the present study was to investigate the presence of different subtypes of Blastocystis spp. among the
patients referred to Velayat hospital of Qazvin province, Iran.
Methods: Overall, 864 stool samples were examined by using formalin-ethyl acetate concentration method and Trichrome
staining. All specimens were cultured in clotted fetal bovine medium. Later, DNA extraction and PCR amplification of 18S
ribosomal RNA gene region was conducted and phylogenetic tree constructed.
Results: The results revealed 7.9% (68/864) of the study population were infected with Blastocystis. Intestinal symptoms
were observed in 61% (36/59) of individuals positive for Blastocystis, with abdominal pain in 58% (21/36) of cases which was
more frequent than other intestinal signs. No significant relationship was observed among the study variables. By molecular
and phylogenetic analysis, three subtypes ST1 (45%), ST2 (30%) and ST3 (23%) of parasite were identified.
Conclusion: This study showed ST1 subtype was the predominant subtype among the positive specimens, meanwhile the
highest haplotype and nucleotide diversity were clarified in ST3 subtype