Qazvin University of Medical Sciences

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    Association of Depression and Body Mass Index with Inflammation Markers in Tehran Oil Refinery Shift Workers

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    Background: Shift working with the sleep disturbance may lead to an increased body weight, depression levels and inflammation. The purpose of this study is to determine the association between depression scores, Body Mass Index (BMI) and inflammation markers among rotational shift workers in one of the Iranian Oil Refineries. Material and Methods: A cross-sectional study was conducted among 189 shift workers aged 21-52 years old with mean 30.58(±6.970) in one of the oil refineries in Tehran city. Studied population was in an 8 h backward shift (from night to morning). Demographic characteristics were gathered by a general questionnaire and anthropometric variables including weight and height were measured for calculating body mass index (BMI), were calculated. 21-items Beck Depression Inventory, which was translated in Persian, was used to assess the depressive symptoms. Level of Cortisol, IL-6 and hs-CRP was measured by 8 ml fasting blood sample, using RIA kit in radioimmunoassay method, R&D (Germany) kit in enzyme-linked immunosorbent method and quantitative diagnostic kit in immunoturbidimetry method produced by Pars Azmoon Company, respectively. Result: This study showed that Il-6 and hs-CRP were higher, although not statistically significant, in workers with depression scores more than 9 in comparison with workers by depression scores equal or below 9 measured by the 21- Item Beck depression inventory. There was inverse association between cortisol level and BMI (PV<0.01) and positive association between CRP and BMI (PV<0.01) and borderline positive association between IL-6 concentration with BMI (PV<0.05). Conclusion: The results of the study revealed that there was a direct association between inflammation factors with Body Mass Index in Tehran oil refinery shift workers. But, the significant relationship between cortisol level, IL-6 and CRP with Shift Work experience were not found

    The serum level of cystatin C does not predict dopaminergic cell death in Substantia Nigra, evidence from 6 hydroxydopamine-induced animal model of Parkinson's disease

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    Introduction: Cystatin C (CysC) is an indicator of renal function, and has been recently reported that associates with neurodegenerative diseases. To investigate the role of this substance in Parkinson's disease (PD), we evaluated the association between serum levels of CysC and other markers of renal function with 6- hydroxydopamine (6-OHDA) induced Parkinsonism in rat. Methods: The 6-OHDA was microinjected into the medial forebrain bundle by stereotaxic surgery. After behavioral tests, immunofluorescence and biochemical studies were carried out to determine the number of survived dopaminergic (DA) neurons in the substantia nigra pars compacta (SNc) and striatal dopamine level. The blood samples were collected from the caudal vain and the heart of animals. CysC was measured using the enzyme-linked immunosorbent assay (ELISA) kits. Results: There was no difference in serum level of CysC between the 6-OHDA treated and control groups, as well as before and after the toxin in the 6-OHDA group. Also, no association was found between CysC and DA neuronal death in SNc or striatal dopamine level. In addition, there was no significant difference in serum levels of creatinine, urea and potassium ions between the control and 6-OHDA treated groups. Conclusion: Since the death of DA neurons in SNc is the main pathophysiological mechanism underlying in the development of both 6-OHDA induced Parkinsonism and PD in human being, CysC and other markers of renal function cannot reflect DA neuronal death and accordingly cannot use for early diagnosis of PD

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