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Pengujian secara In Vitro Oligosakarida dari Ekstrak Tepung Buah Rumbia (Metroxylon sago Rottb.) sebagai Sumber Prebiotik
Get PDFIn vitro analysis of oligosaccharide from extract rumbia fruit (Metroxylon sago Rottb.) as prebioticABSTRACT. Despite a range of commercially available oligosaccharides there is plenty of room to develop new, functionally enhanced prebiotics. current generation of oligosaccharides was not rationally developed. better understanding of factors determining the prebiotic activity of a particular oligosaccharide. Despite the range of commercially available oligosaccharides mixtures (mainly fructo and galacto-oligosaccharides), very few studies are focused on the mechanisms behind the prebiotic activity of particular oligosaccharides. Probably this lack is due to the unavailability of well characterized oligosaccharide fractions for prebiotic function assessment. The objectives of this research were to asses the ability of lactic acid bacteria in fermentation of oligosaccharide and as prebiotic (in vitro). Material used was oligosaccharide of purified rumbia fruit extract. Analysis of oligosaccharide as prebiotic was conducted in vitro using lactic acid bacteria. The lactic acid bacteria consisted Bifidobacterium bifidum, Bifidobacterium animalis, Lactobacillus bulgaricus and Lactobacillus casei Rhamnosus. The growth media for bacteria was a liquid MRS basic medium where glucose was substituted by oligosaccharide of purified rumbia fruit extract. Incubation was in aerob for Lactobacillus and anaerob for Bifidobacterium in incubator 37oC. The lactic acid bacteria was calculated 24-48 hours during incubation periode. The variables observed were: oligosaccharide component, ability of lactic acid bacteria in fermentation of oligosaccharide, and growth of lactic acid bacteria (Lactobacillus and Bifidobacterium). The result showed that the oligosaccharide component from extract rumbia fruit consisted of: sucrose, stacchiose, and raffinose. The result showed that the oligosaccharide extract rumbia fruit was significantly (P0.05) the growth of lactic acid bacteria (Lactobacillus and Bifidobacterium) and fermentation of oligosaccharide. It is concluded that oligosaccharide of rumbia fruit extract could be used as prebiotic
Pengaruh Bakteriosin Produksi Bakteri Asam Laktat Isolat Indonesia terhadap Jumlah Bakteri dalam Susu Pasteurisasi
Get PDFThe effect of bacteriocin produced by Indonesia Isolate of lactic acid bacteria toward total bacteria in pasteurized milkABSTRACT. The aim of this research was to study the ability of bacteriocin (amylocin) produced by Indonesian isolate of Lactic Acid Bacteria (LAB) (Lactobacillus amylovorus US 121) and its combination with nisin in pasteurized milk. The total bacteria was measured by plate count method. The antimicrobial activity of amylosin at 64 AU/ml had the same activity with nisin at 625 AU/ml and its combination between amylosin and nisin at 32 AU/ml and 312,5 AU/ml respectively against psychrotrophic, thermoduric bacteria and L. monocytogenes in pasteurized milk. However, combination of amylosin and nisin (32 AU/ml + 312,5 AU/ml) able to reduce S. aureus in pasteurized milk. Amylosin at 64 AU/ml kept the shelf life of pasteurized milk longer until 12 days at 10oC. The addition of amylosin (the precipitate resulted by ammonium sulfate precipitation at 70% w/v) changed the color of pasteurized milk be brownish white
Daya Hambat Hidrolisis Karbohidrat Oleh Ekstrak Daun Murbei
Get PDFInhibition hydrolysis of carbohydrate by mulberry leaves extractABSTRACT. Mulberry leaves has a great potential as animal feeds because of its high nutrient content, but has deoxynojirimycin (DNJ) active matter. It is potential to inhibit carbohydrate hydrolysis process, come to monosaccharides. The objective of this experiment is to study the inhibiting ability of mulberry leave extract in carbohydrate hydrolysis process. The kinds of carbohydrates were using glucose, maltose, sucrose and starch. This experiment used twenty four of 60 days old male mice (Mus musculus). Diet and water were given ad libitum. Treatment were allocated ina factorial completely randomized design with three replications and two factors containing of completely mulberry leaves extract and variance of carbohydrates. Variable observed were feed consumtion, feed digestibility, body weight gain and blood glucose. The data were analyzed with univariate analysis of variance. The result showed that inclusion of mulberry leaves extract had decrease body weight (P 0,05) and reduce blood glucose (P 0,05)
Determination of Lead Accumulation in Livers and Feces of Kacang Goats Grazing Tsunami Affected Land
Get PDFPenentuan akumulasi timbal (Pb) dalam hati kambing kacang di daerah tsunamiABSTRACT. Tujuan dari penelitian ini adalah untuk mendeteksi akumulasi timbal pada hati dan feces kambing Kacang dan sekaligus mengukur kadar akumulasi timbal tersebut. Lima belas ekor kambing Kacang yang berasal dari rumah potong di daerah Tsunami dan lima belas ekor kambing Kacang yang berasal dari rumah potong di daerah Non-Tsunami. Diikuti dengan lima ekor kambing Kacang dari desa di daerah Tsunami dan lima ekor kambing Kacang dari desa di daerah Non-Tsunami. Ke-empat puluh ekor kambing Kacang tersebut disembelih untuk diambil hati dan feces. Sebelum hati dan Feces tersebut akan dipreparasi untuk analisis akumulasi dari Timbal, dilakukan pengamatan anatomi dari hati dan feces tersebut. Timbal berakumulasi dihati dan feces dari kambing Kacang, baik yang berasal dari daerah Tsunami dan dari daerah Non-Tsunami. Akumulasi Timbal didalam hati kambing Kacang lebih signifikan tinggi di daerah Non-Tsunami dibandingkan didalam hati kambing Kacang di daerah Tsunami. Sedangkan akumulasi Timbal didalam feces kambing Kacang lebih signifikan tinggi di daerah Tsunami dibandingkan didalam feces kambing Kacang di daerah Non-Tsunami
Performa Pertumbuhan Tikus Putih (Rattus norvegicus) yang Diberi Ransum Berbagai Taraf Limbah Udang
Get PDFGrowth performance of white rat (Rattus norvegicus) fed by various of shrimp waste levelsABSTRACT. The objectives of this research are to investigate: (1) The effect of shrimp waste levels in experimental diets on feed intake, daily weight gain, and feed convertion (F/G) of white rats; (2) Optimal level of shrimp waste in white rat diets; and (3) The responses of both male and female white rats to shrimp waste levels. 15 male and 15 female white rats were given pellet ration contain shrimp waste for four weeks. The experimental design used in this research is Completely Randomized Design with 2 x 5 factorial arrangement in 3 replications. The A factor was sex (male and female) and the B factor was shrimp waste levels in ration (0% as control. 5%, 10%, 15%, and 20%). The parameters determined are feed intake, weight gain, and feed convertion of white rats. The results indicate that shrimp waste levels in ration was significantly (P0.05) affect the dry matter intake, whereas the effect on weight gain and feed convertion of white rats was highly significant (P0.01). Sex was significantly affect weight gain (P0.05%), however it was not significance (P0.05%) on dry matter intake and feed convertion of white rats. There were no interaction (P0.05%) between shrimp waste levels in ration and sex of white rats. The conclusion of this research that shrimp waste levels in ration of white rats increase dry matter intake and daily weight gain. Whereas, it decreased feed convertion. The optimal level of shrimp waste in the diet for daily weight gain was 19.44%, while optimal feed convertion 16.62%. Male daily weight gain was higher than female
Evaluasi Penampilan Reproduksi Sapi Perah (Studi Kasus Di Perusahaan Peternakan Sapi Perah KUD Sinarjaya)
Get PDFEvaluation performance reproduction on dairy cattle (Case study in sinarjaya dairy cattle cooperationABSTRACT. Aim of the identification of performance reproduction was to find out the successfully of reproduction management in the dairy cattle firm or farmers. Performance reproduction of dairy cattle include days open, calving interval, service per conception, first service postpartum and length of the pregnancy. All of that performance reproduction values was ideal value and close relation to the production and that values was difference between farmer and depend of management themselves. Sinar Jaya cooperation is one of dairy cattle cooperation, since 1977 with the population about 1554 dairy cattles in year 2006, has been opportunity to find out the performance reproduction of dairy cattle in that cooperation. Dairy cattle about 197 cattles was used as a sample of the treatment, which were minimum two times calving period with the completely recording of reproduction activity. The method of this research was descriptive method and the data was collected as a census method. As a conclusion, reproduction performance for the first service postpartum, days open and calving interval were ideal values but service per conception was bigger than optimal value
Pengaruh Penggunaan Perekat Sintetis terhadap Ransum Ayam Broiler
Get PDFThe effect of using synthetic binder on physical quality of chicken rationABSTRACT. The purpose of this study was learn about the influence added lignosulfonate and bentonite by some processes production on physical characteristics of pellet form ration. The experimental design had used a Randomized Completely Design with 4 x 3 factorial and 3 replocats. Factor A were processes production, i.e : A0 = without processing, A1 = added 5% water spraying, A2 = added 5% hot water spraying and A3 = steam 45 minute on 1000C and pressure 1.7-1.8 kg/cm2. Factor R were added binder, i.e: R0 = control ration, R1 = control ration + 1.25% lignosulfonate and R2 = control ration + 2.5% bentonite. The parameters observed were water content, specific weght, specific density, packed specific density, angle of repose, particle size, water activity and durability of pellet. Result from this study showed some steam process by autoclave 45 minute and added binder lignosulfonate and bentonite was obviously influence specific weight, specific density, packed specific density, angel of repose, particle size, water activity and durability of pellet by percentage was 0.67 cm, 0.68 g/cm3, 0.75 g/cm3, 1.57 g/cm3, 26.990, even the water activity 0.81 wasnt good. Added binder lignosulfonate and bentonite was obviously particle size was 0.67 cm and durability of pellet was 99.99%. Added lignosulfonate and bentonite by some process given the best on durability if it compared with commercials ration. The purpose of this study was to learn about the effect of using binder lignosulfonate and bentonite during six weeks period. The experimental design used a Randomized Completely Design with 3 x 4 factorial and 3 replications. Factor B was storage term (0. 2. 4 and 6 weeks). The parameters observed were water content, water activity factor higroskopic, particle size, specific density, bulk density, packed bulk density and pellet durability. The storage term very significant (P0.01) influenced the water content and increased the water activity, decreased the factor higroskopic, particle size, specific density, bulk density, packed bulk density and durability of pellet. The lowest water content was 10.82% in 6 weeks. The lowest water activity was 0.74 in 0 week. The highest particle size was 0.669 cm in 0 week. The highest durability of pellet was 99.95% in 0 week. The highest specific weight was 1.30 g/cm3 in 2 weeks. The highest specific density was 0.738 g/cm3 in 0 week. The highest packed density was 0.738 g/cm3 in 0 week and the lowest factor higroskopic was 0.79% in 2 weeks
Respon Pertumbuhan Ayam Lokal Pedaging terhadap Suplementasi Protein Isolasi Biji-bijian (PIB) dan Perbedaan Level Protein Ransum
Get PDFThe response of local meat chicken growth to supplementation of isolated grain protein and the difference in ration protein levelABSTRACT. A research which aims to determine the response of local meat chicken growth of protein supplementation with Isolation Grains Protein (IGB) and the difference in ration protein level has been conducted in the Laboratory of Experimental Farm, Animal Husbandry Department, Faculty of Agriculture, Syiah Kuala University-Darussalam, Banda Aceh for 90 days. This study used a completely randomized design factorial with 2 factors, consisting of factors namely male gender (JJ) and female (JB) and the ration is a combination of factors and levels IGB in the ration, ie: treatment A: 17% protein and 0.4% IGB; treatment B 19% protein and 0.6% IGB and treatment C 21% protein and 0.8% IGB. Each combination consisted of 4 replications and each replication consists of 5 chickens. Parameters observed in the study were weight gain, achievement of final weight, consumption, conversion and efficiency of ration. DOC used a derivative result of selection of local meat chicken which are in the process of selection. Data acquired and processed by ANOVA.The results showed that supplementation of IGB and ration protein level difference was significantly effect (P 0.01) on weight gain, final weight, rate of consumption, conversion efficiency of rations and rations, but there is no interaction effect between sex and ration factors . The highest weight gain obtained in the male local chicken achieved by feeding a ration B (93.23 grams), while the hen rations achieved by providing treatment C (63.86 grams / week). The highest final body weight of male chicken on treatment B (1491.5 gram/90 days) and hens in treatment C (1061.5 gram/90 days). However, the highest ration consumption in both male and female local chickens obtained from the ration A. Feed conversion value and the best feed efficiency obtained in treatment B for the treatment of male and C for female chicken. The study concluded that there were different responses between male and female local chickens of the use of IGB in the rations. In male local chicken, IGB is more effective when added in the ration with a protein level of 19% whereas in female at the protein level of 21%
Pengaruh Jenis Susu dan Persentase Starter yang Berbeda terhadap Kualitas Kefir
Get PDFEffect of different milk and starter percentage on kefir qualityABSTRACT. The objective of this study is to study the quality of kefir with different amount of starter added and different kind of milk. The quality of kefir was evaluated by measuring lactic acid level, syneresis, crude protein, crude fat and amount of active microorganism. The result showed that the different percentage of starter and kind of milk has a high significant on syneresis and Lactic Acid level (P 0,01). The best interaction was resulted from the combination of 10% starter and UHT milk. The result also indicated that the different percentage of starter and kind of milk has no effect on crude protein, crude fat and the amount of active microorganism. The combination of 10% Starter and UHT milk, produces the best quality of kefi
Pengaruh Jarak Tanam dan Pemangkasan terhadap Kualitas Silase Dua Varietas Jagung (Zea mays L.)
Get PDFEffects of crop arrangement and cutting on the silages quality of two varieties of maize (Zea mays L.)ABSTRACT. The objective of this experiment was to study the interaction effect of varieties, spacing and cutting on the quality of silage as foodstuff. The experiment had been conducted from January to April, 2007 at the experimental Garden of Faculty of Agriculture, University of Haluoleo. This experiment was arranged in Split-Split plot design with three factors. The first factor was variety as main plot, consisting of BISI-2 variety and local variety; the second factor was space arrangement as sub plot, consisting of 60 cm x 40 cm (J1), 75 cm x 40 cm (J2), 90 cm x 40 cm (J3), the third factor was cutting period as sub plot, consisting of no cutting (Po), cutting on 40 days (P1), and cutting on 55 days (P2). So that there were 2 x 3 x 3 x 3 = 54 experimental units. Analysis of variance was applied to know the effect of treatment. If so, least significant difference (LSD) 0.05 (95 %) confidence level was used. Variables observed was plant growth determining silages quality, consisting of quality of crude protein, crude fiber, crude fat, and water. Results of research revealed that interaction affect of spacing and cutting periods on 40 days of BISI-2 variety gave the best yield of plant growth for plant height. While the local variety tended to adverse effect of cutting. However, the quality of silages of two varieties tended to be similar. Every crop spacing had a positive effect of cutting on 40 days. The best quality of silages of two varieties was resulted from the interaction between spacing 75 cm x 40 cm and cutting of 40 days