Jurnal Agripet
Not a member yet
462 research outputs found
Sort by
Evaluasi Validitas Human Cortisol Enzyme-Linked Immunosorbent Assay (ELISA) Kit dan Waktu Sentrifugasi Sampel Darah untuk Pengukuran Konsentrasi Hormon Kortisol pada Kambing Kacang
Full text linkABSTRAK. Penggunaan human cortisol enzyme-linked immunosorbent assay (ELISA) kit untuk pengukuran hormon kortisol pada hewan dan keterlambatan waktu sentrifugasi sampel darah untuk analisis hormon perlu dievaluasi. Penelitian ini bertujuan untuk mengevaluasi validitas human cortisol ELISA kit (EIA-1887, DRG Instruments GmbH, Germany) untuk pengukuran konsentrasi kortisol dan menguji pengaruh keterlambatan waktu sentrifugasi sampel darah terhadap stabilitas konsentrasi kortisol pada kambing kacang. Sampel darah dikoleksi dari delapan ekor kambing kacang. Uji validitas kit EIA-1887 dilakukan secara: a) analitik (uji parallelism, akurasi, dan presisi), dan b) biologis (pengukuran kortisol sebelum dan setelah transportasi). Uji keterlambatan waktu sentrifugasi terhadap stabilitas konsentrasi kortisol dilakukan dengan 5 perlakuan yaitu disentrifugasi kurang dari 1 jam (P1/kontrol), 6 jam (P6), 12 jam (P12), 18 jam (P18),dan 24 jam (P24) setelah darah dikoleksi. Data uji parallelism dianalisis dengan uji persamaan kemiringan, uji presisi dihitung % CV (coefficient variation) intra-assay dan inter-assay, uji akurasi dihitung % recovery, uji T untuk validasi biologis, dan uji ragam (One Way Anova) untuk pengaruh waktu sentrifugasi. Hasil uji parallelism menunjukkan kurva sampel kambing kacang sejajar/parallel dengan kurva standar kortisol. Akurasi kit EIA-1887 adalah 103,437,85%, dan % CV intra-assay dan inter-assay adalah 10%. Konsentrasi kortisol setelah transportasi secara signifikan lebih tinggi daripada sebelum transportasi (p0,05). Adanya penurunan secara nyata konsentrasi kortisol pada darah yang disentrifugasi 24 jam (P24) setelah koleksi (p0,05). Kesimpulan, human cortisol ELISA kit (EIA-1887) memiliki validitas yang baik secara analitik dan biologis untuk digunakan dalam pengukuran konsentrasi kortisol pada kambing kacang. Keterlambatan waktu sentrifugasi selama 24 jam berpengaruh terhadap konsentrasi kortisol. (Evaluation the Validity of Human Cortisol Enzyme-Linked Immunosorbent Assay (ELISA) Kit and Centrifugation Time of Blood Sample for Measuring the Concentration of Cortisol in Kacang Goats) ABSTRACT. The use of human cortisol ELISA kit for measuring cortisol in animals and delayed to blood centrifugation time for hormone measurement need to be evaluated. This study aimed to evaluate the validity of human cortisol ELISA kit (EIA-1887, DRG Instruments GmbH, Germany) for cortisol measurement and effect of delayed to blood centrifugation time on cortisol concentrations in kacang goats. Blood was collected from eight kacang goats. Validation test of EIA-1887 kit was performed through: a) analytical (parallelism, accuracy, and precision tests), and b) biological validations (measuring cortisol concentrations before and after transportation). Five treatments were performed to test delayed to centrifugation time: blood centrifuged at 1 h (control, P1), 6 h (P6), 12 h (P12), 18 h (P18), and 24 h (P24) after collection. Parallelism data were analyzed by slope equality test, precision and accuracy calculated by % CV of intra-and inter-assay, and % recovery, respectively. Data of biological validation and centrifugation time effects were analyzed by Student t-test, and one way ANOVA, respectively. Results of parallelism showed that serial dilution curve of kacang goat plasma was parallel with cortisol standard curves. Accuracy of EIA-1887 kit was 103.437.85%, and % CV of intra-and inter-assay were 10%. Concentration of cortisol after transportation was significantly higher than before transportation (p0.05). Concentration of cortisol was significantly decreased when blood was centrifuged at 24 h after collection (P0.05). In conclusion, human cortisol ELISA kit (EIA-1887) is a reliable assay for measuring cortisol in plasma of kacang goat. Delayed to blood centrifugation time affect cortisol concentrations
Pengaruh Substitusi Gula dengan Puree Kurma (Phoenix Dactylifera L.) terhadap Sifat Kimia, Mikrobiologi dan Hedonik Es Krim Kefir
Full text linkABSTRAK. Kefir merupakan susu fermentasi yang belum banyak diminati oleh masyarakat karena rasanya yang asam sehingga perlu dilakukan pengembangan pada produk kefir. Salah satunya dengan penggunaan kefir sebagai bahan baku es krim yang diharapkan dapat meningkatkan minat masyarakat mengkonsumsi kefir. Karena rasanya yang asam, es krim kefir perlu ditambahkan pemanis yang dapat diperoleh dari buah buahan salah satunya adalah buah kurma (Phoenix dactylifera L). Buah kurma adalah buah yang memiliki kandungan gula yang cukup tinggi dan memiliki potensi untuk dimanfaatkan sebagai pemanis alami. Penelitian ini bertujuan untuk mengetahui pengaruh substitusi gula dengan puree buah kurma pada beberapa konsentrasi terhadap kadar gula, total padatan, total asam, total khamir, total BAL, total mikroba dan hedonik dari es krim kefir. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan puree buah kurma sebagai pemanis pada beberapa level yaitu 0%, 25%, 50%, 75% dan 100%. Hasil penelitian menunjukkan bahwa substitusi gula dengan puree buah kurma berpengaruh nyata (p0,05) terhadap kadar gula, total padatan, total asam es krim kefir, tetapi tidak memberikan pengaruh yang nyata (p0,05) terhadap total khamir, total BAL dan total mikroba. Substitusi gula dengan puree buah kurma memberikan pengaruh nyata terhadap tingkat kesukaan rasa, warna, tekstur dan overall kesukaan es krim kefir, namun tidak terdapat pengaruh yang nyata terhadap aroma es krim kefir.(Effect of sugar substitution with dates puree (Phoenix dactylifera L.) on the chemical, microbiological and hedonic properties of kefir ice cream) ABSTRACT. Kefir is a fermented milk that has not been in a great demand by people because of its sour taste, so it is necessary to develop kefir products. The use of kefir as a raw material for ice cream is expected to increase peoples interest to consume kefir. Because of its sour taste, kefir ice cream needs to be added with sweeteners which can be obtained from fruits, one of which is dates (Phoenix dactylifera L). Dates are a fruit that has a high enough sugar content and has the potential to be used as a natural sweetener. The purpose of this study was to determine the effect of sugar substitution with date palm puree with several concentrations on the value of sugar content, total solid, total acid, total yeast, total LAB, total microbes and hedonic from kefir ice cream. This study used a Completely Randomized Design (CRD) with dates puree as a sweetener at the level of 0%, 25%, 50%, 75% and 100%. The results showed that the substitution of sugar with date palm puree had a significant effect (p0,05) on the sugar content, total solid, total acid of kefir ice cream, but did not have a significant effect (p0,05) on the total yeast, total LAB dan total microbes. Substitution of sugar with dates puree had a significant effect on the taste, color, texture and overall preference and there was no significant effect on the aroma of kefir ice cream
Evaluasi Status Reproduksi Sapi Hasil Persilangan Peranakan Ongole dengan Bali
Full text linkABSTRACT. Tujuan penelitian untuk mengetahui status reproduksi hasil persilangan sapi peranakan ongole (PO) dan sapi bali (yang selanjutnya dinamakan sapi POBA) jantan dan betina melalui beberapa kegiatan lapang. Penelitian ini bersifat non eksperimental yang dilakukan secara eksploratif; menggunakan 20 ekor sapi berumur antara 32 sampai 36 bulan. Parameter yang diamati meliputi kualitas semen, kondisi organ reproduksi sapi betina dan angka kebuntingan. Hasil penelitian menunjukkan, kualitas semen sapi POBA rendah atau jelek berdasarkan hasil analisis secara mikroskopis maupun makroskopis; namun memiliki libido yang cukup tinggi yaitu rata-rata sekitar 4 menit 25 detik dari mulai mengendus atau didekatkan dengan ternak pemancing (teaser) sampai ejakulasi. Secara umum kondisi organ reproduksi sapi POBA betina normal, sehingga siap dijadikan indukan yang produktif. Sebanyak 66,67% menunjukkan perilaku estrus yang tampak terutama keluar lendir yang terlihat jelas menggantung di bibir vagina bahkan diantaranya mengejar dan/atau menaiki sapi-sapi lain. Namun hasil pemeriksaan kebuntingan (PKB) melalui palpasi rektal menunjukkan tidak ada sapi betina yang bunting. Dapat disimpulkan bahwa sapi POBA jantan infertil, yang diindikasikan melalui hasil pemeriksaan semen dan tidak adanya sapi betina yang berhasil bunting melalui perkawinan alam selama 3 (tiga) bulan. Sedangkan sapi betina POBA memiliki organ reproduksi normal serta menunjukkan ekspresi estrus yang baik. (The evaluation of cattle reproduction status between crosses Ongole Crossbreed with Bali cows) ABSTRAK. The purpose of this research was to determine the reproductive status of the crossbred PO and Bali cattle (hereinafter referred to as POBA cattle) through several field activities. This research is non-experimental and carried out exploratory using 20 cattle aged 32 to 36 months. Parameters observed included semen quality, cows' reproductive organ condition, and pregnancy rate. The quality of POBA bulls semen was low or bad as indicated by the results of microscopic and macroscopic analysis; but had pretty high libido, which was an average of about 4 minutes and 25 seconds, starting to sniff or being brought near to teaser until ejaculation. In general, the reproductive organs of POBA cows were normal so that they were ready to become productive sires. As many as 66.67% showed estrus behavior, especially visible mucus that was hanging on the lips of the vagina, even among them chasing and/or riding other cattle. However, the results of pregnancy examination through rectal palpation showed that there were no pregnant cows. It can be concluded that POBA bulls are infertile, which was indicated by the results of semen examination and the absence of successfully conceived cows through natural mating for 3 (three) months. Meanwhile, POBA cows have normal reproductive organs and show good estrus expression
Kecernaan, Fermentabilitas dan Produksi Protein Mikrobia Secara In Vitro pada Complete Feed Berbasis Pelepah Sawit Fermentasi
Full text linkABSTRACT. Penelitian bertujuan mengetahui kualitas complete feed dengan level pelepah sawit fermentasi berdasarkan kecernaan bahan kering, bahan organik, produksi N-NH3, produksi volatile fatty acids (VFA) dan produksi biomassa protein mikrobia serta protein total secara in vitro. Materi yang digunakan adalah complete feed tersusun atas konsentrat dan pelepah sawit fermentasi dengan berbagai level yaitu 0, 10, 20 dan 30%. Rancangan percobaan yang digunakan adalah rancangan acak lengkap (RAL) dengan 4 perlakuan complete feed dengan level pelepah sawit fermentasi yang berbeda. Data diolah menggunakan sidik ragam yang dilanjutkan dengan uji beda wilayah berganda Duncan. Hasil penelitian menunjukkan bahwa complete feed dengan level pelepah sawit fermentasi yang berbeda berpengaruh nyata (p0,05) terhadap kecernaan bahan kering dan bahan organik, produksi N-NH3, produksi VFA, dan produksi protein total, sedangkan pada biomassa protein mikrobia tidak terdapat perbedaan yang nyata (p0,05). Rata-rata nilai kecernaan bahan kering pada perlakuan T0, T1, T2 dan T3 adalah 69,59; 71,9; 69,05; dan 62,58%. Rata-rata nilai kecernaan bahan organik pada perlakuan T0, T1, T2 dan T3 adalah 63,59; 63,15; 65,50; 52,66 %. Rata-rata produksi VFA pada perlakuan T0, T1, T2 dan T3 sebesar 105,8; 142,7; 136,4; dan 135,7 mM. Rata-rata produksi NH3, biomassa protein mikrobia dan produksi protein total pada perlakuan T0, T1, T2 dan T3 berturut-turut adalah 6,48mM, 15,04mg/ml;, 34,10mg/g; 7,36mM, 15,75mg/ml, 23,72mg/g; 8,18mM, 12,59mg/ml, 33,72mg/g); dan 6,60mM, 15,31mg/ml, 40,80mg/g. Berdasarkan hasil penelitian disimpulkan bahwa penggunaan pelepah sawit fermentasi dengan level 20% dalam complete feed menghasilkan produksi VFA, kecernaan bahan kering dan bahan organik yang cukup baik sehingga dapat menjadi pakan alternatif sumber serat pengganti rumput.(Digestibility, fermentability and in-vitro production of microbial protein on complete feed based on fermented palm frond) ABSTRAK. This study aimed to determine the quality of a complete feed containing fermented palm fronds based on the digestibility of dry matter, organic matter, N-NH3, VFA, microbial protein biomass, and total protein in vitro. The material used was complete feed composed of concentrates and fermented palm fronds at various levels, i.e., 0, 10, 20, and 30%. The experiment was conducted as a completely randomized design (CRD) with four complete feed treatments containing different levels of fermented palm fronds. The data were processed using analysis of variance, followed by Duncans multiple range test. The results demonstrated that the complete feed with different levels of fermented palm fronds had a significant effect (p0.05) on the digestibility of dry matter and organic matter, N-NH3 production, essential fatty acids production, and total protein production, whereas there was no significant difference (p 0.05) on microbial protein biomass. The average dry matter and organic matter digestibility values of T0, T1, T2, and T3 treatments were 69.59; 63.59, 71.9; 63.15, 69.05; 65.50, and 62.58%; 52.66% respectively. The average production of volatile fatty acids of T0, T1, T2, and T3 treatments were 105.8; 142.7; 136.4; and 135.7 mM. respectively, while the average N-NH3 production, microbial protein biomass, and total protein production of the T0, T1, T2, and T3 treatments were 6.48, 7.36, 8.18, 6.60 mM; 15.04, 75, 12.59, 15.31 mg/ml; and 34.10, 23.72, 33.72, 40.80 mg/g. In conclusion, the use of fermented palm fronds at a 20% level in complete feed gave the best result in the production of volatile fatty acids, improved digestibility of dry matter, and organic matter, so it can be used as an alternative feed to replace grass fiber
Pengaruh Pemberian Ekstrak Daun Gaharu (Grynops versteegii) terhadap Stres Transportasi pada Broiler Jantan
Full text linkABSTRACT. Penelitian bertujuan mengetahui pengaruh penambahan ekstrak daun Gaharu (Grynops verstegii) sebelum transportasi terhadap stres transportasi dan penyusutan bobot badan pada broiler jantan. Materi yang digunakan sebagai sampel yaitu 60 ekor dari 200 ekor broiler jantan yang dipelihara, dan ekstrak daun Gaharu dari hasil destilasi. Penelitian menggunakan metode eksperimental dengan Rancangan Acak Lengkap (RAL) dengan uji lanjut Duncans. Broiler jantan yang digunakan dalam penelitian terbagi menjadi 4 perlakuan level pemberian ekstrak daun Gaharu yang berbeda. Pemberian ekstrak daun Gaharu berdasarkan pada bobot badan (BB) broiler, yaitu P0 (tanpa pemberian ekstrak daun Gaharu sebagai kontrol ), P1 (250 mg/kg BB), P2 (300 mg/kg BB), P3 (350 mg/kg BB), setiap perlakuan memiliki lima ulangan dan setiap ulangan menggunakan 3 ekor. Parameter yang diamati adalah penyusutan bobot badan, frekuensi denyut jantung, suhu tubuh, dan frekuensi Pernafasan. Hasil penelitian menunjukkan bahwa penambahan ekstrak daun Gaharu (Grynops verstegii) tidak berpengaruh nyata (P0,05) terhadap persentase susut bobot badan broiler, frekuensi denyut jantung, suhu tubuh dan frekuensi pernafasan. Disimpulkan bahwa pemberian ekstrak daun Gaharu sebelum transportasi pada broiler tidak berpengaruh terhadap penurunan stres setelah transportasi hal ini ditunjukkan oleh perubahan suhu tubuh, frekuensi pernafasan, peningkatan denyut jantung dan penyusutan bobot badan yang tidak berbeda nyata (P0.05) dibandingkan dengan kontrol. (The effect of agarwood leaf extract (Grynops versteegii) on transport stress in male broilers) ABSTRAK. This study aims to determine the effect of adding agarwood leaf extract a source of antioxidants before transportation on body weight loss and the physiological conditions of broilers. The materials used were 60 male broilers as samples and agarwood leaf extract obtained through the extraction process using the distillation method. This study used experimental methods with a completely randomized design (CRD) with Duncan's test. The broilers used in the study consisted of 4 treatment groups with different levels of Gaharu leaf extract, they were P0 (control), P1 (250 mg/kg body weight, P2 (300 mg/kg body weight), P3 (350 mg/kg body weight), each treatment was repeated five times and each repetition used 3 individuals. The parameters observed were weight loss, heart rate frequency, increase in body temperature, and respiratory rate. The results showed that the addition of agarwood leaf extract (Grynops verstegii) had no significant effect (P 0.05) on the percentage of broiler body weight loss, heart rate frequency, increased body temperature and respiratory frequency. It was concluded that the addition of agarwood leaf extract before transportation could not reduce body weight loss and the physiological conditions of broilers
Populasi Bakteri Usus Halus dan Performan Ayam Kampung Silangan Kampung-Leghorn Akibat Ditambahkan Ekstrak Umbi Dahlia dalam Ransum
Full text linkABSTRACT. Penelitian bertujuan untuk mengkaji perkembangan bakteri usus halus dan performan ayam kampung silangan kampung-leghorn (KL) akibat ditambahkan ekstrak umbi dahlia dalam ransum. Ternak percobaan yang digunakan adalah persilangan ayam kampung silangan KL sebanyak 200 ekor umur 22 hari dengan rata-rata bobot badan 180,46 1,21 g. Bahan perlakuan meliputi umbi dahlia, ethanol 70%, kertas saring halus serta bahan pakan penyusun ransum (jagung giling, bekatul, bungkil kedelai, tepung ikan, CaCO3 dan mineral dan vitamin mix). Rancangan percobaan yang digunakan rancangan acak lengkap dengan 4 perlakuan dan 5 ulangan (masing-masing unit percobaan diisi 10 ekor). Perlakuan yang diterapkan meliputi: P0=Ransum kontrol/RK, P1=RK+0,39% ekstrak umbi dahlia, P2=RK+0,78 ekstrak umbi dahlia, dan P3= RK+1,17 ekstrak umbi dahlia. Parameter yang diukur meliputi populasi bakteri asam laktat, Escherichia coli, pH digesta tiap masing-masing segmen usus halus dan pertambahan bobot badan harian (PBBH). Data dianalisis dengan menggunakan uji sidik ragam dan jika berpengaruh nyata dilakukan uji beda nyata Duncan pada taraf 5%. Hasil penelitian menunjukkan bahwa penambahan ekstrak umbi dahlia berpengaruh nyata terhadap populasi bakteri asam laktat, Escherichia coli, pH digesta tiap masing-masing segmen usus halus dan PBBH. Kesimpulan adalah penambahan ekstrak umbi dahlia sampai taraf 1,17% sebagai sumber inulin di dalam ransum dapat meningkatkan populasi bakteri asam laktat, menurunkan pH dan Escherichia coli pada masing-masing segmen usus halus serta memperlambat laju digesta dan meningkatkan PBBH. (Population of small intestine bacteria and performance of native chicken-leghorn crossbreed duo to feeding of dahlia tuber extract) ABSTRAK. This study aims to examine the development of small intestine bacteria and the performances of native chicken-leghorn crossbreed due to the addition of dahlia tuber extract in the diet. Experimental animals were 200 unsex native chicken-leghorn crossbreed, 22 days old with an average body weight of 180.46 1.21 g. Treatment materials include dahlia tuber, ethanol 70%, fine filter paper and feed stuff (yellow corn, rice bran, soy bean meal, fish meal, CaCO3, and vitamin-mineral mix). The present experiment was assigned in a completely randomized with 4 treatments and 5 replications (10 birds each). The treatments were: P0=Control Diet/CD, P1=CD+0,39% dahlia tuber extract, P2=CD+0,78% dahlia tuber extract and P3=CD+1,17% dahlia tuber extract. Parameters measured were the number of Lactic Acid Bacteria (LAB), Escherichia coli, intestinal potential hydrogen (pH) (duodenal, jejunal and ileal), rate of passage and daily body weight gain (DBWG). Data were subjected to ANOVA and followed by Duncan Multiple Range Test on 5% level. The results indicated that feeding native chicken-leghorn crossbreed with the diet containing dahlia tuber extract (DTE) significantly (P0.05) increased LAB population and DBWG, decreased E. coli population and intestinal pH, and slow the rate of passage down. In conclusion, feeding of dahlia tubers as a source of inulin in the form of dahlia tuber extract to a level of 1.17% increased the population of lactic acid bacteria, reduced pH and Escherichia coli in each segment of the small intestine and retard the rate of digestion and increased body weight gain
Pengaruh Pemberian PGF2 Sebelum Koleksi terhadap Peningkatan Kualitas Semen dan Level Testosteron Sapi Aceh
Full text linkABSTRAK. Penelitian ini bertujuan mengetahui pengaruh pemberian hormon PGF2 sebelum koleksi terhadap peningkatan kualitas semen dan level testosteron sapi Aceh di UPT. Hewan Coba Universitas Syiah Kuala. Penelitian ini dilaksanakan dari bulan Januari sampai dengan Maret 2019. Data hasil pemeriksaan kualitas semen dianalisis secara deskriptif. Dalam penelitian ini digunakan satu ekor sapi Aceh jantan sebagai kontrol dengan 2 perlakuan, yaitu P1 (tanpa pemberian PGF2 sebelum koleksi) dan P2 (pemberian PGF2 30 menit sebelum koleksi) dengan dosis 25 mg tiap perlakuan. Setiap perlakuan dilakukan sebanyak lima kali dan dilakukan secara bergantian setiap minggunya. Rata-rata volume (ml); konsentrasi (106 sel/ml); motilitas (%), viabilitas (%); abnormalitas (%); dan level testosteron (ng/ml) pada P1 vs P2 masing-masing adalah 5,21,30 vs 5,20,97 (P0,05); 145,436,58 vs 172,8 46,27 (P0,05); 63,876,44 vs 75,050,08 (P0,05); 69,300,22 vs 75,050,08 (P0,05); 16,900,08 vs 10,460,09 (P0,05); dan 28,0714,37 vs 24,196,11 (P0,05). Disimpulkan bahwa pemberian PGF2 30 menit sebelum koleksi dapat meningkatkan kualitas spermatozoa tetapi tidak dapat meningkatkan konsentrasi testosteron. (Case study the effect of giving pgf2 before the collection of the quality of Aceh cattle semen) ABSTRACT. This study aims to determine the effect of giving PGF2 hormone before collection to improve the quality of Acehnese semen in UPT. Hewan Coba Syiah Kuala University. This research was conducted from January to March 2019. Data on semen quality examination were analyzed descriptively. In this study, one Aceh cattle was used as a control with 2 treatments, namely P1 (without administration of PGF2 before collection) and P2 (administration of PGF2 30 minutes before collection) at a dose of 25 mg per treatment. Each treatment was carried out five times and carried out alternately every week. The results showed that the average volume of cement (ml) and pH P1 and P2 respectively 5.2 1.30 and 5.2 0.97, and 6.60.55 and 6.5 0.50. While the average concentration of spermatozoa (106 cells / ml) in P1 and P2 were 145.4 36.58 and 172.8 46.27 respectively. The average percentage (%) of progressive spermatozoa motility, circularity, non motility, viability, and abnormalities in P1 and P2 respectively: 63.87 6.44 and 73.63 9.42, 11.34 3, 70 and 9.0 7.01, 24.79 4.04 and 17.37 4.44, 69.30 0.22 and 75.05 0.08, 16.90 0.08 and 10.46 0.09. It was concluded that administration of PGF2 30 minutes before collection can increase the quality of spermatozoa but cannot increase the concentration of testosterone
Efek Penambahan Ruminer dalam Ransum Sapi Perah terhadap Produksi 4% FCM dan Nutrien Susu
Full text linkABSTRACT. Sapi perah yang berproduksi tinggi membutuhkan ransum berenergi tinggi dan asam lemak esensial. Penambahan lemak dalam ransum dapat mengatasi kekurangan energi dan asam lemak esensial. Permasalahan umum dari pemberian lemak atau minyak pada dosis tertentu dapat mengganggu ekosistem rumen, sehingga dalam pemberiannya perlu diproteksi. Ruminer adalah suplemen komersial berupa minyak sawit yang diproteksi mineral kalsium. Penelitian ditujukan untuk mengetahui penambahan ruminer dalam ransum lengkap terhadap produksi susu 4% FCM dan produksi nutrien susu sapi perah. Penelitian dilakukan secara eksperimental terhadap 10 ekor sapi perah Friesian Holstein pada masa laktasi sama. Sapi tersebut dibagi dua, masing-masing lima ekor. Perlakuan berupa ransum tanpa ditambah ruminer (P0) dan ransum ditambah 250 gram ruminer.ekor-1.hari-1 (P1). Peubah yang diamati adalah produksi susu 4% FCM diukur setiap hari selama 60 hari dan kualitas susu diukur setiap 10 hari. Hasil penelitian menunjukkan bahwa produksi susu 4% FCM, produksi total padatan, lemak, protein dan laktosa susu pada sapi diberi ruminer lebih tinggi (P0,05) daripada sapi yang tidak diberi ruminer. Disimpulkan bahwa pemberian ruminer meningkatkan produksi susu 4% FCM, total padatan, lemak, protein dan laktosa susu. Pemberian ruminer sebanyak 250 gram.ekor-1hari-1 mampu meningkatkan 2 kg produksi susu 4% FCM pada sapi perah laktasi.(Effects of ruminer addition in dairy cattle rations on milk 4% FCM and nutrient production) ABSTRAK. High-yielding dairy cows require high-energy rations and essential fatty acids. The addition of fat in the ration can overcome the lack of energy and essential fatty acids. The general problem of giving fat or oil at certain doses can disrupt the rumen ecosystem, so that it needs to be protected. Ruminer is a commercial supplement in the form of calcium mineral protected palm oil. This research was aimed to determine the addition of ruminants in complete rations on the production of 4% FCM milk and the nutrient production of dairy cow milk. The research was carried out experimentally on 10 dairy cows during the same lactation period. The cow is divided into two, five each. The treatments were rations without adding ruminer (P0) and rations added by 250 grams of ruminer. head-1 day-1 (P1). The variables observed were 4% FCM milk production measured every day for 60 days and milk quality was measured every 10 days. The results showed that the production of 4% FCM milk, the total production of solids, fat, protein and milk lactose in cows fed with ruminer was higher (P 0.05) than cows that were not given ruminer. It was concluded that the provision of ruminer increased milk production by 4% FCM, total solids, fat, protein and milk lactose. Giving a ruminer of 250 grams of head-1.day-1 was able to increase 2 kg of 4% FCM milk production in lactating dairy cows
Dampak Pemberian Mikroenkapsulasi Minyak Ikan dalam Pakan terhadap Kolesterol Darah dan Performa pada Domba
Full text linkABSTRAK. Tujuan penelitian ini untuk mengukur seberapa besar pengaruh minyak ikan yang sudah terenkapsulasi terhadap kolesterol darah dan performa pada Domba. Penelitian telah dilakukan di Sub Unit Pelayanan Pengembangan Pembibitan Ternak Domba dan Kambing (SUPPPTDK) Bunihayu, Subang pada tanggal 20 Januari 2020 sampai 27 Maret 2020. Sebanyak 18 ekor domba Ekor Tipis jantan dengan bobot 15,990,98 kg dialokasikan ke dalam 3 perlakuan secara acak. Domba tersebut diberi ransum perlakuan yang disuplementasi dengan mikroenkapsulasi minyak ikan sebanyak 0% (P0), 2,5% (P1), dan 5% (P2). Data yang terkumpul dilakukan analisis ragam dan bila hasil berbeda nyata maka dilanjutkan dengan uji Duncan. Hasil menunjukkan bahwa mikroenkapsulasi minyak ikan dapat menurunkan kolesterol darah (P0,05), namun tidak berpengaruh nyata terhadap pertambahan bobot badan, konsumsi dan konversi ransum (P0,05). Penurunan kadar kolesterol terjadi pada kelompok domba dengan perlakuan mikroenkapsulasi sebanyak 2,5% (P1) dan mikroenkapsulasi 5% (P2). Kadar kolesterol darah masing-masing perlakuan berturut-turut adalah 100,70 mg/dl (P0); 96,20 mg/dl (P1); dan 78,76 mg/dl (P2). Rataan yang terbaik pada pertambahan bobot badan, konsumsi bahan kering harian, dan konversi ransum terdapat pada P2 yaitu 63,96 g/hari, 574,13 g/hari, dan 9,08. Hasil penelitian dapat disimpulkan bahwa mikroenkapsulasi minyak ikan dapat menurunkan kolesterol darah, dengan penurunan tertinggi pada pemberian 5%. (The impact of microencapsulation fish oil in feed on blood cholesterols and performance on sheep) ABSTRAK. This study measured the effect of encapsulated fish oil on blood cholesterol and sheep performance. The study was conducted at the Bunihayu Sheep and Goat Breeding Services Bunihayu, Subang on January 20, 2020 to March 27, 2020. A total of 18 male thin-tailed sheep 15.990.98 kg were allocated randomly into 3 treatments. The sheep were given feed supplemented with fish oil microencapsulation of 0% (P0), 2.5% (P1), and 5% (P2). The data were collected and analyzed by analysis of variance and, if the result is significantly different, continued by Duncan's test. The results showed that fish oil microencapsulation decreased blood cholesterol levels (P0.05). However, it did not affect body weight gain, consumption, and feed conversion (P0.05). Decreased levels of cholesterol occurred in the group of sheep with 2.5% microencapsulation (P1) and 5% microencapsulation (P2). Blood cholesterol levels of each treatment were 100.70 mg/dl (P0); 96.20 mg/dl (P1); and 78.76 mg/dl (P2), respectively. Averagely, the highest body weight gain, dry matter intake and feed conversion were found in P2 as much as 63.96 g/day, 574.13 g/day, and 9.08, respectively. The results can be concluded that microencapsulation of fish oil can reduce blood cholesterol, with the highest decrease at the level of 5%
Pengaruh Penambahan Pakan Ampas Kelapa yang Difermentasi dengan Penambahan Ammonium Sulfat terhadap Produksi dan Kualitas Telur Ayam Ras
Full text linkABSTRACT. Penelitian bertujuan untuk mengetahui pengaruh penambahan pakan ampas kelapa (AK) yang difermentasi dengan penambahan ammonium sulfat (AS) terhadap produksi dan kualitas telur telah dilaksanakan. Ampas kelapa ditambahkan AS dengan konsentrasi 0, dan 4% dan 0.1% Ragi (Fermipan) dan diinkubasi selama 5 dan 7 hari. Substrat yang dihasilkan dicampur kedalam pakan dan diberikan kepada 75 ekor ayam petelur fase bertelur umur 20 minggu selama 24 minggu. Pakan perlakuan yang diberikan adalah: pakan kontrol (KTL), kontrol + AK tanpa AS yang difermentasi selama 5 hari (F5-0AS), kontrol + AK dengan 4% AS yang difermentasi selama 5 hari (F5-4AS), kontrol + AK tanpa AS yang difermentasi selama 7 hari (F7-0AS) dan kontrol + AK dengan 4% AS yang difermentasi selama 7 hari (F7-4AS). Penelitian ini menggunakan rancangan acak lengkap dengan 5 perlakuan, 5 ulangan dan 3 ekor ayam per ulangan. Data yang diperoleh dianalisis dengan analisis ragam dan dilanjutkan dengan uji Tukey. Hasil penelitian menunjukkan bahwa konsumsi ransum, produksi telur, bahan kering ekskreta dan kualitas telur dipengaruhi oleh pakan perlakuan (P0,05). Perlakuan tidak memberikan pengaruh yang nyata (P0,05) terhadap bobot telur, kecernaan bahan kering, persentase kerabang telur, indeks telur dan tinggi albumen. Total bobot telur tertinggi diperoleh pada ayam yang diberi perlakuan fermentasi baik tanpa ataupun dengan penambahan AS. Fermentasi selama 7 hari dapat meningkatkan produksi henday dan jumlah telur. Kualitas terbaik dari aspek indeks kuning telur dan Haugh unit adalah pada perlakuan F7-4AS. Penelitian dapat disimpulkan bahwa fermentasi dapat meningkatkan total bobot telur dan AS dapat meningkatkan kualitas telur dalam bentuk Haugh unit. (The effect of coconut dregs fermented with the addition of ammonium sulfate on production and quality of eggs) ABSTRAK. An experiment was done to determine the effect of coconut dregs (CD) with the addition of ammonium sulfate (AS) prior to fermentation on productive performance and egg quality. Coconut dregs were added with 0 and 4% AS and 0.1% yeast S. cerevisiae (Fermipan) after autoclaving. The substrates were incubated for 5 and 7 days and mixed into diets and offered to 75 laying hens for 24 weeks. The experimental diets were: control diet (KTL), basal + 5-days- fermented coconut dregs (FCD) without AS (F5-0AS), basal + 5-days FCD with 4% AS (F5-4AS), basal + 7-days- FCD without AS (F7-0AS), basal + 7-days-FCD with 4% AS (F7-4AS). Data were analyzed using analysis of variance with 5 treatments and 5 replications. Data were further tested by the Tukey test. Results of the study indicate that feed intake, hen day production, total egg, total egg mass, excreta dry matter, yolk height, yolk index, and the Haugh unit were statistically affected by the treatments (P0.05). The effects of treatments were not statistically significant (P0.05) on individual egg mass, dry matter digestibility, eggshell percentage, egg shape index, and albumen height. The highest total egg mass was found in birds fed the FCD diets. Fermentation for 7 days could improve hen day production total egg. The higher yolk index and Haugh unit were produced by the hens fed the F7-4AS diet than those eggs produced by controlled laying hens. In conclusion, fermentation could improve total egg mass and AS supplementation could increase the Haugh unit