Jurnal ILMU DASAR
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Flooded Area Mapping and Its Relationship to the Land Use, Soil Type, and Rainfall in North Konawe Regency
Full text linkThe flood incident in North Konawe Regency, Southeast Sulawesi that occurred on June 2nd, 2019 was the largest flood disaster in that area since the last 42 years, so it is interesting to study. As part of disaster risk management, it is necessary to do flood mapping to determine the distribution of flooded areas and identify areas that have potential for flooding. Mapping of flood inundation areas was carried out using Sentinel-1 data. Land use, rainfall and soil types are used as an analysis of their were relationship to the distribution of flood. The distribution of flood based on the identification of the presence of inundation covered 3 sub-districts, namely Oheo District, Asera District and Andowia District. Correlation of flood distribution to the land use, rainfall and soil type was identified using Pearson correlation value (r). The correlation between flood distribution and land use was -0.59 that indicates the correlation is moderate. Moreover, the correlation of flood distribution to the rainfall was 0 which means the correlation was very weak, and lastly, the correlation value of the flood distribution with soil type was 0.88 or the correlation was very strong
The Binding Prediction of 6-Paradol and its Derivatives on TRPV1 Agonist as a New Compound for Treating Painful Diabetic Neuropathy
Full text linkGinger was reported to have a suppressive effect on pain in patients with Painful Diabetic Neuropathy (PDN). Our latest study revealed that 6-shogaol, one of the ginger components, had the best affinity in the Transient Receptor Potential Vanilloid 1 (TRPV1), a key receptor in PDN). Paradol, which obtained from gingerol and shogaol metabolism, also had potent activities in several diseases, compared to the other derivatives of gingerol and shogaol. However, shogaol and paradol is very similar in chemical structure with only different in one double bond in 4-5 position. Until now there is no explanation about paradol mechanism in TRPV1. Based on this, our study was designed to predict the activity of 6-paradol and its derivatives to TRPV1 as target receptor in PDN using in-silico model. 2-paradol, 4-paradol, 6-paradol, 8-paradol and 10-paradol were used as ligands. Capsaisin, the agonist of TRPV1, was used as a native ligand in this study. TRPV1 was obtained from protein data bank (PDB). Ligand bond prediction and affinity was performed using Molegro Virtual Docker. The results showed 2-paradol, 4-paradol, 6-paradol, 8-paradol and 10-paradol had good affinity against TRPV1. These result indicated that 6-paradol and the derivatives had potential as a drug compound for PDN therapy
The 32 kDa Outer Membrane Proteins of Klebsiella pneumoniae Acts as A Bacterial Adhesin
Full text linkKlebsiella pneumoniae is a bacterium that often causes infection in the human body. At present K. pneumoniae can resist some of the antibiotics it has associated with modification of one of the virulence factors possessed by K. pneumoniae. One virulence factor of K. pneumoniae as pathogen bacteria is Outer Membrane Protein (OMP). The study of adhesin factors in K. pneumoniae involving hemagglutinin and adhesin proteins that have been found in the OMP 20 kDa and 40 kDa, but there is still no research that discusses the role of 32 kDa OMP as a hemagglutinin protein and adhesin. The purpose of this study is to determine the role of 32 kDa outer membrane of K. pneumoniae as hemagglutinin and adhesin proteins. After isolation of Outer Member Protein (OMP) from the K. pneumoniae, which then carried out a hemagglutination test using mice erythrocyte cells and adhesion test using mice enterocyte cells. The results of the hemagglutination test using mice erythrocyte cells obtained the highest hemagglutination titer for the molecular weight of 32 kDa in titers 1/4. The adhesion index with dilution titer has a significant relationship, with a conversion coefficient of 0,813 which means the dilution titer with the OMP adhesion index has a strong relationship with the direction of a positive relationship. The regression test results obtained an R-value of 0,813 which shows a strong relationship, while the R2 value is 66.1%. Conclusion in this study is 32 kDa outer membrane proteins of K. pneumoniae acts as a bacterial adhesin
Optimized Purification of CIDRα-PfEMP1 Plasmodium falciparum Recombinant Protein with Affinity Chromatography
Full text linkInteraction of Cysteine-rich Interdomain Region (CIDR)α-Plasmodium falciparum Erythrocyte Membrane Protein (PfEMP1) and Endothelial Cell Receptors especially CD36 on host cells is main malaria pathogenesis, makes this domain as a malaria vaccine candidate. Recently, the development of the malaria vaccine is conducted by recombinant technology, and the purification of the CIDRα-PfEMP1 recombinant protein is a pivotal step. This study aimed to determine an optimal condition to purify the CIDRα-PfEMP1 recombinant protein by affinity chromatography through imidazole and NaCl concentration. The purified recombinant protein was visualized using SDS-PAGE and its concentration was measured using Image J software and Bradford Assay. The data were analyzed using SPSS 26 software, and the Paired T-Test analysis was conducted to compare the concentration of purified recombinant protein from two different methods. The result showed that thetarget band of purified recombinant protein was 27 kDa. The thickest target protein band was observed in purified recombinant protein using 140 mM imidazole and 300 mM NaCl. The recombinant protein concentration using Image J software was 0.025 µg/µL, while the Bradford Assay was 0.56 µg/µL. The Paired T-Test analysis has a significance value of 0.010 (p<0.05), meaning there was a significant difference between the concentration measurement using Image J software and Bradford Assay. In conclusion, the optimized condition to purify the CIDRα-PfEMP1 recombinant protein by affinity chromatography was using 140 mM imidazole and 300 mM NaCl. It is suggested to measure the purified CIDRα-PfEMP1 recombinant protein concentration using the Bradford Assay method due to its convenience and sensitivity
Effect of Black Soybean (Glycine soja) Extract on Ovarian Histology of Swiss Webster Mice (Mus musculus L) Unilateral Ovariectomy
Full text linkEstrogen production can be reduced due to menopause and ovariectomy. Decreased estrogen levels in the body cause physiological changes in the female reproductive system. Therefore, the intake of estrogen from outside the body is needed, in this case phytoestrogens from soy bean extract can be used to replace the role of endogenous estrogen. The study used 30 Swiss Webster mice (Mus musculus) aged 90 days weighing 35 grams which were unilateral ovariectomy. Mice are ready to be used as test animals after an estrogen deficiency period of 60 days. Mice are ready to be used as test animals after an estrogen deficiency period of 60 days. Mice were divided into five groups: negative control, positive control, dose 0.21, 0.42, and 0.63 g/mL/day . Data obtained from the results of the study were analyzed using the One Way ANOVA test with a confidence level of 99% or α = 0.01. Based on the results of the study, the administration of black soybean flour extract in unilateral ovariectomy mice can increase the average number of primordial follicles at doses of 0.42 g/ml/day and dosage 0.63 g/ml/day, primary follicles at a dose of 0.42 g/ml/day and doses of 0.63 g/ml/day, and secondary follicles at a dose of 0.63 g / ml / day, and tend to increase the average number of primordial follicles at doses of 0.21 g/ml/day, follicles primary at doses of 0.21 g/ml/day, secondary follicles at doses of 0.21 g/ml/day and 0.42 g/ml/day, de Graaf follicles at doses of 0.21, 0. 42 and 0.63 g/ml/day
Antioxidant of Effervescent Tablet Formulated from Combination of Secang Wood and Red Ginger Extracts
Full text linkSecang wood has strong antioxidant properties because it contains phenolic compounds such as flavonoids and brazilin. Also, red ginger has antioxidant derived from nonvolatile phenolic compounds. Until now, there has been no use of a combination of secang wood extract and red ginger specifically for the manufacture of effervescent tablets. The purpose of this study was to formulate an effervescent tablet by doing combination of secang wood and red ginger extracts with using various concentrations of citric and tartaric acids. The formulations conducted were three acid sources of 20%, 25%, 30%, using wet granulation. The evaluation subjected to the formulas included the physical properties of the granules, such as the water content and flow properties of the granules. Meanwhile, the evaluation performed to the tablet included the hardness test, tablet friability, weight uniformity, dissolution time, and acidity (pH). The analysis was carried out in accordance with the requirements of the Indonesian Pharmacopoeia Edition III and BPOM. The results obtained are effervescent tablet with an acid variation of 20% with physical quality values of water content of 2.91% and granule flow properties of 6.03 s. Meanwhile, the physical quality requirements of effervescent tablets were tablet hardness of 4.02 kp; tablet friability 0.46%; weight uniformity 0.132%; 71 s dissolution time; and 6.4 degrees of acidity
Biological Aspects of Three Species Family Gobiidae in the Bilah River, Labuhanbatu Regency, Sumatera Utara Province
Full text linkThe Bilah river is known to have a fairly high fish biodiversity. Some species of Gobiidae are usually caught by fishermen in the estuary area. But until now there is no data and information related to the biological aspects of the Gobiidae family. The purpose of this study was to determine aspects of the size class and long relationship of the weight of Gobiidae fish caught by fishermen in the Bilah river. This study is exploratory, where fish sampling stations are determined based on fish habitat from fishermen's information (purposive sampling). Fish are caught using traditional fishing gear in the form of splint. The fish caught is then measured in total length and weight. Then the fish obtained based on the catch are grouped in small, medium, and large sizes. Data analysis using Microsoft Excel 2010 applications. In this study, three species of fish that are included in the family Gobiidae namely Butis butis, Butis aureus, and Eleotris fusca. The small category B. butis (9-11.9 cm) fish size class is obtained as many as 2 individuals, medium size (12-14.9 cm) 9 individuals, and large size (15-17.9 cm) 2 individuals. The small size class (6-8.9 cm) of B. aureus fish obtained 2 individuals, medium (9-11.9 cm) 8 individuals, and large 12-14.9 cm) 4 individuals. Small size class (9-11.9 cm) E. fusca as many as 6 individuals, medium (12-14.9 cm) 7 individuals, and large (15-17.9 cm) 3 individuals. The growth pattern of the crazy fish (B. butis) (value b = 0.10), B. aureus (value b = 0.27), and E. fusca (value b = 0.20)