Jurnal ILMU DASAR
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Antioxidant Isosantosimol from Garcinia griffithii Stem Bark
Get PDFA polyisoprenylated benzophenone, isosantosimol had been isolated from the stem bark of Garcinia griffithii T. Anders. The structure of these compound was determined on the basis of spectroscopic data including UV, IR, 1H NMR, 13C NMR, HMQC, HMBC and COSY, as well as by direct comparison with those of reported data. The antioxidant activity of this compound was observed using radical scavenging 1,1-diphenyl-2-picryl hydrazyl (DPPH) model systems
Cloning, Sequencing and Characterization of The Xylan Degrading Enzymes from Geobacillus thermoleovorans IT-08
Get PDFGeobacillus thermoleovorans IT-08 is a Gram positive, thermophilic bacterium that can utilize xylan as a sole source of carbon. This strain was isolated from Gunung Pancar hot spring, Bogor, West Java, Indonesia. A plasmid genomic library in Escherichia coli DH5α was constructed and screened for xylanase activity. One positive clone, namely DH5α (pTP510) has been isolated, sequenced and showed putative exo-xylanase (exo-xyl), β-xylosidase (xyl), and α-L-arabinofuranosidase (abfa) genes (Genebank Accession No.DQ387047, DQ345777 and DQ387046 respectively). Each gene encoded 604, 511 and 502 amino acids, respectively. The BLAST search for protein database revealed that Abfa was high similar with GH51 family Abfa of Geobacillus stearothermophilus T6, but Xyl and Exo-Xyl were slight similar with GH43 family (25-34%) respectively. The deduced protein had a molecular weight of about 70 kDa (Exo-Xyl), and 60 kDa (Xyl and Abfa). These showed good accordance with the calculated molecular weight of each protein (68.64 kDa for Exo-xyl, 57.99 kDa for Xyl and 57.03 kDa for Abfa) from deduced amino acid sequence
Interaction Mechanism of Fe with Dietary Fiber Component at in vitro Gastrointestinal System Condition
Get PDFThe aim of the research was to describe the interaction mechanism of Fe with yard long bean dietary fiber macromolecules at gastrointestinal system in-vitro, through the combination of acidity and boiling time. The research based on the factorial experimental design, with two independent variables, i.e. acidity medium (pH 3, 4, 5, 6, 7)and boiling time (0/raw, 5, 20, 35 minutes). Dependent variables were iron binding percentage, effective stability constants (Keff), and adsorption constants (Kads). Scatchard and Langmuir graph methods through Keff and Kads were then applied to determine the mechanism of iron interaction. The results of the research showed that the highest binding Fe by dietary fiber at pH 7- raw, and lowest at pH 3- boiling time 35 minutes. The iron binding pattern by dietary fiber at gastrointestinal system is through formation of complex compound that more prominent than physically adsorption, and involved two types of specific binding sites, one of which showed a higher affinity than the other
An Isoflovonoid, Warangalone from the Stem Bark of Dadap Ayam (Erythrina variegata)
Get PDFIn the course of our continuing search for novel paralytic compound from Indonesian plants, the methanol extract of the stem bark of Erythrina variegata (Leguminosae) showed significant paralytic activity against the third instar larvae of silkworm (Bombyx mori). The purposes of this research were isolation and structural elucidation of paralytic compound from the stem bark of E. variegata. Using the paralytic activity following the separation, the methanol extract was separated by combination of column chromatography to yield prenylisoflavone, warangalone. The chemical structure of warangalone was identified based on spectroscopic evidence and comparison with the previous reported. The paralytic activity of warangalone showed weak activity against the third instar larvae of silkworm (B. mori)
Preliminary Investigation: StearidoStudi Pengaruh Pengadukan dan Tanpa Pengadukan Larutan Elektrolit Terhadap Struktur Kristal, Morfologi dan Rasio Magnetoresistansi Lapisan Tipis Paduan NiFe Hasil Elektrodeposisinic Acid Production by Genetically Modifie
No full textNi-Fe alloy thin film on Cu substrate by electro-deposition method has been made without as well as with agitation of 300 rpm for a 3 minutes deposition time and potential of 3 Volt. The characterizations consist of crystal structure test by X-Ray Diffraction method, morphology and composition test by SEM/EDX and magnetoresistance measurement with two-point probes equipment. Result of the XRD test showed that the growth of crystal structure was in the same orientation on 111, 200, 220, 311with structure of fcc. Morphology test showed that the structural images of Ni-Fe surface thin film on agitation became flat and had less grain size. The composition test results showed that composition with agitation closely Ni80Fe20 (permalloy) was 87.51% for Ni and 12.49% for Fe. The magnetoresistance measurement was less with the agitation treatment i.e. without agitation was 9.1 % and with agitation was 1.9 %
Production and Characterization of Enzyme β-Endoxylanase from Bacteria of Termite-intestinal System
Get PDFA mesophile bacteria isolated from intestinal systems of local-soil termite, produced an extracellular β-endoxylanase upon growth on xylan. Optimum production of the enzyme was found in media containing oatspelt xylan at 37oC after sixth hours. The activities of its β-endoxylanase on oat-spelt xylan was investigated. It had an optimum pH and temperature, 5.0 and 40o C, respectively. However, pH stability occurred between 5.08.0. The enzyme was stable at 40o C for four hours and possessed a half life of four hours. β-endoxylanase had an apparent molecular mass of 45.000 to 66.200 Dalton as determined by SDS-PAGE. Analysis of zymogram using SDS-Xylan-PAGE indicated that enzymes could degrade oat-spelt xylan as substrates
Isolation and Characterization of the Expression of Gene for Sucrose Transporter Proteins in Sugarcane Plant (Saccharum officinarum)
Get PDFSucrose as the major transported form of fixed carbon must be translocated from source tissue to the sites of consumption and storage or sink tissues. The translocation of sucrose is facilitated by some distinct sucrose transporters proteins (SUT). To study sucrose transporters in sugarcane, we had conducted isolation and characterization of gene encoding sucrose transporter protein. The isolation was performed with RT-PCR method using total RNA isolated from sugarcane leaf and primer designed from conservative region of SUTcDNAs of SoSUT2A (accession number AY65599), OsSUT-1(accession number AAP54842, OsSUT-1 mRNA (accession number XM 46477). Based on the conservative amino acids sequences of QILQQFA and MGKTEPV, the corresponding sequences of the primers for RT-PCR were: P1, (forward) 5’CAGATCCTTCAACAGTTCGC-3’ and P2 (reverse) 5’-TGCCCTTTGTCTCCGGAACC-3’, respectively. Agarose gel electrophoresis shown a clear single 0.5 kb cDNA band of the PCR product. Thus, the DNA was cloned into pGEMT vector (Promega) for further analysis. Sequence determination of the PCR product revealed a nucleotides sequence of 543 bp in length and has a high homology around 89%, 87.3 % and 84.8 % with maize ZmSUT-1, sugarcane SoSUT 2A and rice OsSUT-1 mRNA, respectively. We designated the cDNA as SoSUT2 and the nucleotide sequence have been submitted to GenBank data base under accession number bankit 734628. By using PSORT analysis the fragment of cDNA-SoSUT2 encoded protein may be located in the endoplasmic reticulum. To have a better understanding, the expression of SoSUT2 gene in sugarcane was determinate by RT-PCR method using total RNA isolated from leaf, petioles, stem and root and visualized the PCR product in agarose gel electrophoresis. Based on the cDNA bands intensity, it can be illustrated that the expression of SoSUT2 gene were found highest in sugarcane leafs then petioles and stem, but the expression was not found in root. Although the SoSUT2-cDNA has not been isolated in full size, the results suggest the presence of gene family of SUT in sugarcane
The Use of Nata de Coco Membrane as Biosensor Material
Get PDFNata de coco has been characterized as filter toward glucose solutions. Standard nata de coco membrane can not be used as membrane because of variations of thickness. It has unequal weight if it was made thinner. Based on its character, nata de coco had been made in various incubation times. Three days incubation time membrane have passed up 76.84 % glucose, 61.15 Lm -2hr -1 flux/permeability. In Low concentration, it’s produced permeate 87% and decrease in increasing concentration. Structures of membrane morphology showed that existence of bovine serum albumin (BSA) in glucose solution possibility didn't influence determination of glucose in blood
Data Non-normality on AMMI Models: Box-Cox Transformations
Get PDFAMMI (Additive Main Effect Multiplicative Interaction) model for interactions in two-way table provide the major mean for studying stability and adaptability through genotype × environment interaction (GEI), which modeled by full interaction model. Eligibility of AMMI models depends on that assumption of normally independent distributed error with a constant variance. In the case of non-normal data distribution, the appropriateness of AMMI model is being doubtful. Transform the observation by power family of Box-Cox transformation is an effort to handle the non-normality. AMMI model then can be applied to the transformed data appropriately following by the use of ordinary least square for estimating parameters. This approach is investigated by applying them to (i) a count data of pest population of Poisson distribution, which came from a study of leave pest in soybean genotype, and to (ii) a study of rice genotype stability of filled grain per panicle (Binomial data). One must be carefully considered what the meaning of the transformation in the AMMImodels and Biplot AMMI
Effect of Substituting Pure Sucrose by Sugarcane Juice as Carbon Source on the Fermentation of Dextran Production
Get PDFSucrose is a carbon source for dextran fermentation and it is also used as a substrate of dextransucrase enzyme for producing dextran. Sugar cane juice containing sucrose as a main sugar, hence it is potential to be used as a cheap medium for dextran fermentation. This research was conducted to study the dextran fermentation using sugar cane juice as a medium. Two main experiments were done in this research. The first experiment has been carried out to determine the optimum medium composition for dextran fermentation using pure sucrose as a carbon source by variations on type and concentrations of yeast extract and buffering minerals. The second experiment was conducted to determine the effect of substituting pure sucrose in the fermentation medium by sugar cane juice. Fermentation was conducted at static condition, room temperature and 16-20 h fermentation time. The results showed that the optimum conditions for dextran fermentation using pure sucrose were sucrose 20%, yeast extract 0.75% (technical grade yeast extract was able to be used) and K2HPO4 minerals for buffering medium. Dextran production was able to reach 51 mg/g medium. The optimum medium composition and fermentation conditions were used as a control medium. In the second experiment, pure sucrose in the control medium was substituted by sugar cane juice with variations of 0; 50, 75 and 100%. Technical grade yeast extract was still added at 0.75%. The result showed that the higher sugar cane juice concentration the lower dextran production in the fermentation. On the other hand, medium fermentation containing 100% sugar cane juice without yeast extract was able to produce 45 mg dextran/g medium, which was not significantly different to dextran production from control medium. This experiment showed that sugar cane juice was a potential material as a cheap carbon source for dextran fermentatio