Jurnal ILMU DASAR
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Studies Polymorphism of Blood Plasm Protein in Gurami (Osphronemus gouramy Lac) in Jember County
Get PDFThe objective of this research was to study the blood protein polymorphism of gurami (Osphronemus gouramy Lac). Blood sample were collected from 55 gurami. Polyaacrilamide Gel Electrophoresis (PAGE) was used in the blood plasm protein analysis. Identification of protein bands on gel are determinated by comparing the molecul weight of those test group protein with the marker protein. The result showed that the locus albumin, pre-albumin and transferin are polymorphic. Locus albumin, pre-albumin and transferin are controlled by three alleles ie A, B and C. The total heterozygozity of gurami is 52 %
The Treatment of the Corn-Knob as A Raw Material for Making Furfural
Get PDFThis research is conducted to determine the furfural rendement of the corn-knob with the varieties of heating periods of 2; 2.5; 3; 3.5; and 4 hours. This methods used were, first, the hydrolysis of the corn-knob using HCl with the varieties of heating periods as mentioned above. Then it was continued by the extraction and purification of the furfural through distillation. The resulted furfural is identified by the acetic-anilin color reaction, infrared spectrophotometer, refractive index and GC-MS. The identification result was compare with the furfural standard. The result of this research shows that average rendement was 8.43% for the heating period of 2 hours, 9.67%, for 2.5 hours, 11.57% for 3 hours, 13.30% for 3.5 hours and 12.59% for 4 hours
Analysis of Blood Flow in Arterial Stenosis Using Casson and Power-Law Fluid Model
No full textSimulation of blood flow behaviour in the arteries and in arterial stenosis has been made and will be discussed in this paper. This simulation uses pulsatile flow and blood flow in artery without stenosis is considered as a dynamic fluid, compressed and condensed. Whereas, in the case of arterial stenosis has been used Casson and Power-law fluid models. In the arteries without stenosis, blood flow velocity profiles show the same pattern for each Womersley number, but with different speed value. In the case of arterial stenosis, blood flow rate decreases with increasing stenosis position away from axis of blood vessels. Resistances to flow are increases with increasing the size (height and length) of stenosis, both for the Casson and Power-law fluid models. If resistance to flow increases, it is more difficult for the blood to pass through an artery, result the flow decreases and heart has to work harder to maintain adequate circulation.Keywords : Artery, blood flow, power-law fluid, Casson fluid, stenosis Â
Preparation of Reference Material for Trigonelline Determination Inside of Green Coffee Beans Using HPTLC
Get PDFPreliminary study of preparation reference material for trigonelline determination in green coffee beans have been performed by HPTLC method. Physical characterization performed on green coffee beans include the determination of moisture content, ash content and metal content. Water content values obtained on average 6.4% (%RSD 4.8) and ash content 3.9% (%RSD 16.2). Metal determination (Cu, Zn, Fe) with atomic absorption spectrometry method found Cu 9.4 (mg/kg), Zn 12,0 (mg/kg) and Fe 64.0 (mg /kg). Sonication as extraction method using methanol solvent for 30 minutes and separation method HPTLC on aluminum plates coated with silica gel 60 F254. Eluent used was mixture of n-propanol: methanol: water (4:1:4, v/v/v) with elution length 8 cm. Linear regression obtained in concentration range 200-1200 ng spot-1 with R2 = 0.9857. Detection limits obtained 140.7 ng and 468.9 ng for quantitation limit. Repeatability was achieved from 800 ng spot-1 (% RSD 13.8). Keywords : Reference material, Trigonelline, green coffee, HPTL
Finite Difference Method and Newton's Theorem to Determine the Sum of Series
Get PDFProblems that are often faced to prove the truth of a formula if the presented series is a series that is not the formula of arithmetic and geometric series. One proof among the most commonly proofs used is the proof by mathematical induction. This study was conducted to determine the sum of the first n terms formula of: (1) arithmetic series, storied arithmetic series with the basis of arithmetic series, (2) geometric series, (3) storied arithmetic series with the basis of geometric series, and (4) series which are not arithmetic and geometric series that the formula of the n terms is given, by using the finite difference method and Newton's theorem. The formula of the sum of the first n terms obtained from the results of this study and then it is verified by using mathematical induction. Keywords : Series, finite difference, mathematical induction, Newton’s theore
The spesies composition and distribution pattern of Gastropod at Forrest Mangrove Block Bedul Segoro Anak, Alas Purwo National Park
Get PDFThis research aims to determine the spesies compossition and distribution pattern of Gastropod and its relationship with abiotic factors (pH, salinity, soil texture, and organic content of the soil, and the high of tide of the eustuary area) in the Mangrove forest at Blok Beduk Segoro Anak Alas Purwo National Park. This research was conducted on February 2013. Data was taken four times using a week time interval during one month. Every observation covered eight stations, where station 1 to station 4 consist of four transects and 40 plots, while station 5 to station 8 consist of 3 transects with 42 plots and each plot was 5 m × 5 m. Gastropod observed from each plot are preserved with 70% alcohol and identified in Malakologi Laboratory, Indonesian Institute of Sciences (LIPI). Results of the research reveated that the Gastropod observed consist of 19 families and 37 species, and the dominant family is Ceritidae. The Diversity index of Shanon Wiener was 0.53 (low deversity). Two available of Distribution pattern, group (Canarium labiatum, Cassidula nucleus, Cerithium coralium, Chicoreus brunneus, Cassidula vespertilionis, Cerithidea cingulata, Cerithidea quadrata, Chicoreus capucinus,Conus rattus, Conus striolatus, Ellobium aurisjudae, Littorina carinifera, Littorina scabra, Monodonta labio, Nassarius melanoides, Nassarius olivaceus, Nerita balteata, Nerita planospira, Nerita undata, Pugilina ternatana, Sphaerassiminea miniata, Telescopium telescopium, Terebralia sulcata, Thais intermedia), random (Angaria delphinus, Conus catus, Conus omaria, Cymatium moniliferum, Erronea errones, Oliva oliva, Polinices aurantius, Pollia undosa, Tectus pyramis, Trochus californicus, Turbo argyrostoma). The abiotic factors had relatianship not significantly (p = 0.067) on Gastropod distribution pattern. Keywords : Distribution pattern,Gastropod, species compossitio
Resistance against Bacillus thuringiensis endotoxins in a laboratory population of Helicoverpa armigera is based on an elevated immune status
No full textRecent  observations  suggest  that  lectin-like  toxins,  such  as  endotoxins  from Bacillus  thuringiensis are sequestered inside the gut of immune-induced insects providing tolerance to Bt-formulations. To investigate the induction  and  sequestration  process,  we  used  toxin  and  lectins  that  bind  to  galactosamine  (Gal)  and  N-acetylagalactosamine (GalNAc) to identify binding sites in the midgut of induced and non-induced insects using confocal  microscopy.  Our  observations  suggest  that  immune  and  metabolic  hemolymph  components  are transported across the gut epithelium and accumulate inside the gut lumen in Bt-tolerant insects. Co-location of immune components and toxin indicates that some of the toxin is inactivated by coagulation reactions inside the gut lumen before it can reach the brush border membran
Design of Supercritical Water Reactor with Thorium Fuel Cell
Get PDFThe research of desain of super critical water reactor (SCWR) thorium fuel has been done. The objective of this research is to obtain the design with high thermal power and inherent safety features. The analyzed parameters were:corefuelenrichment,reactorsize,criticality,andpowerdensitydistribution.Acorecalculationof x,y,z was achieved by CITATION code. Fuel composition and core configurations in critical condition were calculated by variation of fuel enrichment, reactor size and configuration of the fuel in the core. SCWR used thorium as fuel, stainless steel as cladding, light water as moderator and coolant as well. This research obtained critical core design that is size x = 85 cm, y = 85 cm and z = 180 cm. The critical condition can be achieved when the inner fuel loaded about 2.23% fuel enrichment and outer fuel 1.5% fuel encrichmant. The design produced 1000 MW thermal power, the maximum power density is 625.675 Watts /cc and k-effective value is 1.000274. Thereactorcoreinthisresearchfulfillstheinherentsafetystandardintermofneutronicaspect. Keywords : Core design, SCWR, thorium, power density, passive safet
The 35,2 kda Hemaglutinin Protein of Pili’s Proteus mirabilis P355 as Adhesin on Rabbit’s Blader Epitelial
Get PDFUrinary tract infection represent one of nosocomial infection in hospital. One of agent nosocomial urinary tract infection is Proteus mirabilis bacteria and common occur on patient with urinary catheter. Urinary tract infection with caused by P. mirabilis was persistent, very difficult to eradicated. Further more is caused some complication such as cystitis, acut and cronic pyelonephritis, kidney bladder stone, bacterimiae and sepsis. This bacteriae has same virulence factors. Fimbriae is one of it. In the adhesion test utilization of protein hemaglutinin pili 35.2 kDa resulted in the electroelusion which salluted in vesica urinaria epitelial by dose 400μl , 200μl, 100μl, 50μl, 25μl, 12,5μl and 0μl as control. Form adhesion test on vesica urinary epitelial that salluted with 35.2kDa protein pili we faund that the higher dose of protein can decrease bacteriae consentration at vesica urinary epitelial. This result was significant with r= 0.93 and p value = 0.005. This conclusion of this study is 35.2 kDa molecular weight protein pili of P. mirabilis P355 were adhesion protein.Keywords : hemaglutinin, pili, Proteus mirabilis, adhesi
Screening and Identification of Alkaloid Compounds in Kayu Kuning Stem (Arcangelisia Flava Merr)
Get PDFExtraction of kayu kuning stem (Arcangelisia flava Merr) with petroleum ether, chloroform and methanol 80% to screen of alkaloid compound has been carried out. Extract of chloroform was added by 10% of NH3 solution and Al2O3, then put into column chromatography and flowed by chloroform. Eluat was tested by dragendorf reagant to know the present of the alkaloid compound. This eluat was identified by thin layer chromatography with chloroform-methanol (1:4 v/v) as a mobile phase. It gave four spots that had the Rf value of 0.78; 0.64; 0.41; 0.18. After these spots were added by dragendorf reagant, only the first spot gave positive result. Then the chloroform fraction was separated by column chromatography to take the fraction which had Rf value of about 0.78 and thenit was analyzed by spectroscopic method (uv-vis and infra red). The data suggested that the alkaloid compound was the pyridin alkaloids group