Journal of Agromedicine and Medical Sciences
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The Correlation Between The Use of Personal Protective Equipment (PPE) and Soil-Transmitted Helminths Infection in the Workers of Kaliputih Plantation Jember Regency
This study aimed to investigate the correlation between the use of Personal Protective Equipment (PPE) and the status of Soil-Transmitted Helminths infection in the workers of Kaliputih plantation in Jember Regency. The research method applied was Analytic observational with a Cross-sectional approach. The population, as well as the sample, were all workers of Kaliputih Plantation, Sumber Bulus Village, Ledokombo District, Jember Regency, by the total of 63 people. The sampling technique was the Total sampling. Laboratory analysis on faecal samples of respondents were conducted at the Parasitology Laboratory, Faculty of Medicine, University of Jember. Furthermore, the research data were analyzed using Chi-Square analysis or Fisher’s Exact Test. The results demonstrated that the prevalence of STH infection in Kaliputih Plantation workers was 25%. This figure was supported by the good awareness of the use of Personal Protective Equipment (PPE) of workers (91.67%). In addition, the Soil-Transmitted Helminths species that infested the plantation workers were Ascaris lumbricoides, found in 6 people (16.67%), and Hookworm, found in 3 people (8.33%). Finally, the result of Chi-square analysis showed a significance value of <0.05. Therefore, it can be concluded that the use of PPE in Kaliputih plantation workers had a significant correlation with the STH infection status.
Keywords: Correlation, PPE, Soil-Transmitted Helminths Infectio
The Difference Incidence of Maternal Uterine Inertia Between Labor With and Without Anemia in Hospital of dr. Soebandi Jember
One of the indirect causes of maternal death is anemia. Anemia in pregnancy is a woman with a hemoglobin level below 11 g / dl caused by iron deficiency in pregnant women so that the hemoglobin level becomes low and cannot meet the need for oxygen to perfusion to the tissues. This consumes energy in the affected muscles and buildup of lactic acid which causes fatigue and reduced muscle contraction during labor. Inadequate uterine contractions are called uterine inertia and are characterized by a prolonged phase of labor, which is weak, rarely and short duration. The purpose of this study was to determine the differences in the incidence of uterine inertia between labor and anemia released in RSD dr. Soebandi Jember. This study was analytic observational with a cross sectional research design. The study population was all pregnant women who had improved labor and were included in the medical record at RSD Dr. Soebandi Jember period January 1, 2017 - December 31, 2017. Samples from this study were mothers who had approved the delivery and fulfilled the inclusion and exclusion criteria that had been determined until the number of samples could be fulfilled. The number of samples in this study were 76 samples. The type of data used in this study is secondary data obtained by researchers from the medical records of pregnant women who have received labor. The data included in this study contain demographic data consisting of the age of pregnant women and parity, clinical data in the form of uterine inertia and laboratory data consisting of anemia status. In analyzing the data with Chi Square test (X²), a significance value of 0.011 was obtained so that it can be concluded that Ho is rejected and Ha is accepted which means that the incidence of uterine inertia between free labor and anemia is not important in RSD Dr. Soebandi Jember.
Keywords: anemia, uterine inertia, pregnancy, age, parity.
 
Bacterial Mapping Toward Patients in Intensive Care Unit Dr. Soebandi Jember Hospital
Intensive Care Unit (ICU) a part of the hospital with specialized staff and equipment specially to the observation, care and treatment of patients suffering from life threatening complications. The importance of knowing the mapping of bacterial is to know the types of bacterial and the resistance of bacterial to the therapy that will be given. The research used is descriptive by taking primary data. Criteria of research sample in the form of blood culture in patients in ICU RSD. Dr. Soebandi Jember. Sampling method is by using total sampling technique. The results is S. xylosus (55.60%), S. epidermidis (11.10%), Enterobacter cloacea (11.10%) and Stenotrophomonas maltophilia (11.105). Sensitivity test results of Staphylococcus xylosus bacteria showed resistant to class III cephalosporin group, Erythromycin, penicillin, clindamycin and macroloid. While the sensitive to chloramphenicol, linezolid, glikopeptida and vancomycin. The results of the antibiotic sensitivity test of Staphylococcus epidermidis, Enterobacter cloacea, and Stenotrophomonas maltophilia are sensitive to tetracycline and aminoglycoside antibiotics. Meanwhile, antibiotic sensitivity test of Staphylococcus epidermidis and Streptococcus sp are resistant to antibiotic penicillins, fourth generation cephalosporin, erythromycin, azithromycin. Streptococcus sp and Stenotrophomonas maltophilia are resistant to ceftriaxone antibiotic
The Analysis of Osteoblast Cell Number on Femur Fractures Provided Red Spinach Extract (Amaranthus tricolor L.)
Bone fracture is a musculoskeletal injury with a high incidence rate. The healing process of the fracture can be inhibited by oxidative stress, which occurs due to Reactive Oxygen Species (ROS) that exceeds the antioxidant capacity in the body that neutralizes it. Antioxidants that have red spinach have the potential to suppress the level of oxidative stress. This study aims to determine the effect of red spinach ethanol extract on the healing process of fracture in male Wistar rats through osteoblast cell count. A sample of 30 male Wistar rats was divided into five groups; negative control group, positive control group, and three groups of red spinach ethanol treatment with doses of 35.4 mg / 150 g body weight (BW), 70.8 mg / 150 gBW, and 141.6 mg / 150gBW induced fracture, splinted, then treated for one week. One Way Anova test results showed a significance of 0.000 (p <0.05) in which there was a significant difference in the osteoblast level between the treatment group and the control group. The result of LSD test between P1 group and P3 group showed significantly different result where the increase of osteoblast cell number was in line with the increase of the dose of red spinach ethanol extract. The conclusion is that red spinach ethanol extract has a positive impact during the fracture healing process with osteoblast cell number parameters.
Keywords: Red spinach ethanolic extract, fracture healing process, osteoblast, oxidative stres
Correlation of Cholinesterase Levels to Lung Function in Farmer Exposed by Organophosphate Pesticides in Sukorambi Village, Jember Regency
Abstract
Most of the Indonesia population work as a farmer. Pesticides are used in agriculture sector for pest and plant diseases. Pesticides can cause intoxication. The World Health Organization (WHO) estimates that 1-5 million cases of pesticides poisoning among agricultural workers occur in developing countries. Indonesia pesticide poisoning cases reach 771 cases. Organophosphate enter the body through inhalation. Organophosphate pesticides work systemic to inhibit the cholinesterase enzyme causing lung function disorders. The purpose of this study was to analyze the correlation of cholinesterase levels to lung function in farmers exposed to organophosphate pesticides in Sukorambi Village, Jember Regency. This is an observational analytic study using a cross sectional design. Cholinesterase levels test using the DGKC method to determine the presence of poisoning, while for lung function test using spirometry with FVC, FEV1 and FEV1 / FVC ratios as parameters. The results of the test in 30 samples showed 14.33% (4/30) abnormal cholinesterase levels or decreased. Lung function test showed 20% (6/30) had obstructive disorders, 43.33% (13/30) restrictive disorders and 36.67% (11/30) were normal. Data analysis using chi square showed a significant relationship (p = 0.049) between cholinesterase levels and lung function. Conclusion: There is a significant relationship between the level of cholinesterase to decrease in pulmonary physiology of farmers exposed to organophosphate pesticides in Sukorambi Village, Jember Regency.
Keyword :Organophosphate, cholinesterase level, lung function
 
The Relationship Between Clean Water Sources And The Incidence Of Diarrhea In Kampung Baru Resident At Ngagelrejo Wonokromo Surabaya
Abstract
Water is a good medium for bacterial growth, so that contamination is happening especially if less attention in terms of hygiene and sanitation. The use of bed water sources can increase the risk of diarrhea. Diarrhea is one of the environmentally based disease is a major cause of morbidity and mortality. Kampung Baru village located around Jagir Kali Surabaya Most people are in the middle to low socioeconomic level. The lifestyle of them, also pays less attention to environmental hygiene and sanitation, so that it can cause water pollution which can increase the risk of diarrhea. This study was an observational analytic cross sectional study. Entire study population villagers of Kampung Baru. A sample of 75 respondents drawn with simple random sampling technique. The data used is primary data obtained through the distribution of questionnaires, interviews, and observations. Data analysis using the Spearman rank test with a significance level (α = 0.1). The results showed that 53,3% affected diarrhea and 46,7% didn’t affected (P =0.087, r=0.463). The results showed a medium correlation between the source of water with the incidence of diarrhea in Kampung Baru Resident At Ngagelrejo Wonokromo Surabaya.
Keyword : Diarrhae, Water Sanitatio
Difference between Location of Soil with Risk of Contamination of Soil-transmitted Helmints Eggs and Larvae (Observational Study of Coffee Plantation Area in Silo District, Jember Regency)
Soil-transmitted Helminths (STH) is a group of worms whose life cycle through the soil. The species of STH are roundworm (Ascaris lumbricoides), whipworm (Trichuris trichiura), hookworms (Necator americanus and Ancylostoma duodenale), and Strongyloides stercoralis. STH causes helminthiasis that infects more than 1.5 billion people or 24% of the world's population. Jember has many plantation areas. One of the plantations in Jember is Garahan Kidul plantation located in Sidomulyo village, Silo Sub-district, Jember. Soil on plantations tends to be moist and loose. This condition is ideal for the development of STH eggs and larvae. The general purpose of this study was to differentiate the numbers of soil contamination by STH eggs and larvae in soil samples in the garden area, riverside, and worker housing taken in the coffee plantation area in Silo District, Jember. This type of research is observational analytic with cross sectional research design. Soil samples are taken in the garden area, riverside, and worker housing that has a loose or not hard and moist soil texture. The results of the observation found that there were 4 eggs of Ascaris lumbricoides, 6 eggs and larvae of hookworm, and 2 eggs and larvae of Strongyloides stercoralis. There is the same amount of soil contamination at the location of the garden and riverside, which are 2 positive samples or 5.71% with a density of 0.02 eggs and larvae / gram of soil, while the number of soil contamination at the workers housing location is 8 positive samples or 25.71 % with a density of 0.11 eggs and larvae / gram of soil. Data were analyzed using Fisher's test. The results of data analysis showed a value of p = 0.0693 (p> 0.05) so that there were no differences in the number of soil contamination by STH eggs and larvae in the garden and river bank locations and p = 0.042 (p <0.05) so that there were differences the number of soil contamination by STH eggs and larvae at the location of worker housing with the location of garden and riverside.
Keywords: STH, garden, riverside, wooker housin
Protective Effect of Cassava Leaf Extract on Gentamicin-Induced Hepatotoxity In Mice
Abstract
Gentamicin usage can cause the damage of liver structure and function. The basic mechanism inducing liver damage from gentamicin is lipid peroxidation in cell membrane and the suppresion of antioxidant defence system in liver. Antioxidant in cassava leaf such as vitamin C, carotene, flavonoid, dan mineral can protect liver from drug toxicity effect. This research aimed to determine hepatoprotective effect of cassava leaf through microscopic observation of liver histopathology slide of mice induced by gentamicin. The research design was post test only control group design. Mice were divided into five groups, normal group, positive control (gentamicin 80 mg/kg b.w.); P1, P2, and P3 (gentamicin 80 mg/kg b.w. and cassava leaf extract 150 mg/kg b.w., 300 mg/kg b.w., 450 mg/kg b.w. respectively, for 14 days). The average score of liver cell damage was determined by microscopic observation of 200 liver cells undergoing parenchymal degeneration, hidrophic degeneration, and necrosis. One Way Anova analysis showed significant difference among the groups (p<0,05) and Post Hoc Tukey HSD test showed that cassava leaf extract at the dose level of 450 mg/kg b.w. resulted significant liver cell characteristic improvement in liver histophatology slide (p<0,05) compared to positive control group. It could be concluded that cassava leaf extract had protective effect on gentamicin-induced hepatotoxicity in mice.
Keywords: cassava leaf extract, hepatotoxicity, gentamicin, liver histopatholog
Analysis of Morphology and Erythrocyte Count in Rat after Electrical Exposure
Electrical burns cause high mortality and morbidity and various complications, although the incidence is only 4% of all burns. Joule heating phenomenon occurs in electric burn patients which cause changes in erythrocyte morphology resulting hypercoagulability that contribute to the formation of thrombus. Changes in erythrocyte morphology may cause tissue oxygenation disorders and decrease in erythrocyte count leading to hypoxia. Until now there have been no reports of changes in erythrocyte morphology and reduction in the number of erythrocytes in burn patients due to electricity. This study aims to observe and analyze morphological and erythrocyte count in rats after electrical exposure of 140 V for 17 seconds. This is a true experimental laboratories study with post-test only control group design. The sample used were 24 rats consisting of one control group (K) and five treatment groups (P). Treatment groups were distinguished by days of blood sampling that is days 0 (P1), 3 (P2), 7 (P3), 10 (P4), and 14 (P5) post electrical exposure of 140 V for 17 seconds. Assessment in erythrocyte morphology was performed using giemsa staining that observed with 1000x magnification while erythrocyte count was performed using improved neubauer counting chamber. One Way Anova showed that there was no significant change in rats erythrocyte count after electrical exposure of 140 V for 17 seconds (p = 0.110). Descriptive analysis showed that there is morphological changes in erythrocyte morphology after electrical exposure of 140 V for 17 seconds, in terms of shape there are more fragmentosit, microsite, tear drops, stomatocytes, and cell target, in terms of size there are more microsite, and in terms of color there are more hypochromic erythrocytes.
Keywords: Burn Injury, Erythrocyte Morphology, Erythrocyte Coun
The correlation between Mother's Knowledge About Language Stimulation and Language Development Of Toddlers in Lengkong, Mumbulsari, Jember
Abstract
The first three years of age is an important period for the development of children’s languange. Early stimulation as a parent especially mother to promote children’s development needs to be done. Before doing stimulation, the mothers need sufficient knowledge about development stimulation so mothers can do stimulation properly to their children. The aim of this study is to analyze the correlation between the mother’s knowledge about language stimulation and language development of toddlers in Lengkong , Mumbulsari, Jember. An analitic observational study with cross sectional study design which the subjects were 70 mother who have toddlers at Lengkong, Mumbulsari, Jember who met inclusion and exclusion criteria. The result of spearman correlation test between mother’s knowledge about language stimulation and language development of children aged 1-3 years is p=0,000 (p<0.005) which mean there is significant correlation between the mother’s knowledge about language stimulation and language development of children aged 1-3 years in Lengkong , Mumbulsari, Jember.
Keywords: knowledge, stimulation, language development