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The Relationship Between Stress and Temporomandibular Disorder in Students of the Faculty of Dentistry, Baiturrahmah University Class of 2020
Get PDFBackground: Stress is a physiological response in the form of worry or mental tension in the face of a difficult situation. With high study demands, dental students often experience stress. Uncontrolled stress can have adverse effects on physical and bodily functions, one of which can increase the risk of temporomandibular disorder (TMD). Stress can trigger muscle hyperactivity which will cause fatigue in the temporomandibular joint.Methods: This research is an analytic observational study with a cross-sectional design. Respondents of this study were 95 students of the Faculty of Dentistry, Baiturrahmah University Class of 2020. Stress assessment was measured by the Student-Life Stress Inventory (SSI) questionnaire and temporomandibular disorder assessment was measured by the Helkimo Index (Anamnestic Index and Dysfunction Index). Results: The level of stress experienced by 69 people (72.6%) mild stress and 26 people (27.4%) moderate stress. Anamnestic index results were 47 people (49.5%) experiencing mild symptoms, 23 people (24.2%) experiencing severe symptoms and 25 people (26.3%) without symptoms. The results of the dysfunction index as many as 48 people (50.5%) experienced mild dysfunction, 40 people (42.1%) experienced moderate dysfunction and 2 people (2.1%) experienced severe dysfunction. The results of the chi-square test showed p=0.000 (p<0.05) there was a relationship between stress variables and temporomandibular disorder variables. Conclusion: There is a relationship between stress and temporomandibular disorder in Baiturrahmah University Faculty of Dentistry students class of 2020
The Correlation Between Caries Severity and Dental Pain Stress Level in the Community of Sucopangepok Village, Jelbuk Subdistrict, Jember Regency
Get PDFBackground: The results of the Basic Health Research in 2018 show that the prevalence of caries in Indonesia is high (88.8%), and the prevalence of active caries in East Java is also high (42.4%). One of the consequences of untreated caries is stress dental pain due to toothache which has an impact on the quality of life.Method: Observational analytical research with a cross-sectional approach on people aged 17 years and above was conducted on February 2023 – March 2023. A total of 318 respondents uses simple random sampling. The variable of the research are dental caries severity (using the PUFA index) and dental pain stress (using a modification of the Kessler Psychological Distress Scale questionnaire with Perceived Stress Scale).Result: The data obtained were tabulated and analyzed with descriptive statistics and continued with the Spearman correlation test. 36% of respondents age 26-35 years, 57.23% were female. 41.82% have primary school education and work as housewives and farmers. The average caries severity is 2.6 categorized as high with dental pain stress categorized as medium. Spearman correlation test show significance (p) <0.05 which means there is a correlation between caries severity level and dental pain stress.Conclusion: There is a relationship between caries severity and stress dental pai
Effect Of Final Irrigation Materials And Techniques On Sealer Penetration Depth Into Dentinal Tubule Of The Apical Third Root Canal
Get PDFBackground: The smear layer may interfere with the penetration of the root canal sealer. Final irrigation is known to improve chelating agents' ability in smear layer removal. This study aimed to compare the effect of various materials and final irrigation techniques on the penetration depth of bioceramic silers in the apical third of the tooth.Method: This study used 45 premolars that were prepared with a rotary instrument and then randomly divided into three experimental groups (n=15). Group 1 used 17% EDTA as the final irrigation material, group 2 used Novel Silver Citrate, and group 3 used 0.2% nanoparticle chitosan. Each group was divided into three subgroups (n=5), with subgroup A using manual agitation technique, subgroup B using sonic agitation, and subgroup C using ultrasonic agitation. The teeth were then obturated using a bioceramic sealer. Teeth then cut vertically for observation and measurement sealer penetration using a stereo microscope with a magnification of 30x followed by observation using SEM at 1000x magnification. data where analyzed using a two-way ANOVA followed by an LSD Post Hoc test, with a significance level of 95%.Result: Two-way ANOVA showed significant differences in materials, techniques, and material-technique interactions (p<0,05).Conclusion: This study concludes that there is an effect of material, technique, and material-technique interaction of final irrigation on the penetration of bioceramic sealer into the dentinal tubules, Combination of 0.2% chitosan nanoparticles and sonic agitation as material and final irrigation technique resulted in the most longest penetration of bioceramic sealer into dentinal tubules
Cytotoxicity analysis of alginate impression materials based red seaweed extract on cultured gingival fibroblast cells
No full textBackground: Alginate is an impression material that is widely used in dentistry. Alginate can actually also be obtained from natural materials such as red seaweed. The impression procedure causes the impression material to come into contact with oral tissues including the gingiva. Ideally, the materials used must also meet requirements such as low toxicity or non-toxicity so that tissue damage does not occur. This study aims to analyze the cytotoxicity of alginate impression materials from red seaweed extract in gingival fibroblast cell. Method: This experimental laboratory design using post-test only control group design. The research groups consisted of: sodium alginate extract group, red seaweed extract-based alginate impression material, positive control and negative control. Cytotoxicity was tested on gingival fibroblast cell cultures and the effect was analyzed using the MTT assay. Exposure to gingival fibroblast cell cultures was differentiated in three time durations: 5 minutes, 10 minutes and 15 minutes. Each time duration was repeated three times. MTT-formazan production is a method used to measure cell viability (living cells). The data obtained were statistically analyzed using two-way ANOVA test and Tukey HSD post hoc test. Result: There was no significant difference in the average cell viability between the red seaweed extract-based alginate impression material group and the negative control group at an exposure duration of 5 minutes, which was more than 90%. Conclusion: The red seaweed extract-based alginate impression material has no toxic effect on gingival fibroblast cells at 5 minutes exposure
Caries Risk Assessment Using the "CARA" Application in Students of Candijati 01 Elementary School in Jember
No full textBackground: The key to successful treatment to prevent dental caries in children is accuracy in determining the risk factors that cause dental caries. Caries risk is a person's chance of having several caries lesions over a certain period of time. Measuring the risk of caries is carried out so that preventive measures can be directed directly at people who have a higher risk of cariesMethod: Research used is analytical observational with a cross sectional approach. The total population in this study were 170 students aged 6-12 years at Candijati 01 Elementary School. The results of filling out the questionnaire by respondent, dietary survey sheet and also a clinical examination of the oral cavity will be entered into the CARA application to obtain caries risk level for each individual and will display all data in a bar chart.Result: Based on this study, 53,5% students in Candijati 01 elementary School is low caries risk. Based on the indicators used in the CARA application, the results showed that caries indicators and dental treatment history play an important role in causing dental caries. Meanwhile, dental health indicators act as predisposing factors for dental caries.Conclusion: Based on the CARA analysis found that 6-12 years old students of Candijati 01 Elementary School who had were at a low risk category, but still having medium potential of avoiding new caries occurrence by controlling risk factors. CARA Application can be used as caries risk assessment because this application is easy to use and simpl
THE EFFECT OF IMMERSION DURATION OF NON-DENTAL GLASS FIBER REINFORCED COMPOSITE IN ARTIFICIAL SALIVA ON TENSILE STRENGTH
No full textBackground: The type of fiber often used in dentistry is E-glass fiber, which has limited availability and relatively high prices. Non-dental glass fiber can be employed as an alternative in dentistry because its composition is nearly identical to E-glass fiber. Exposure of restorative materials to artificial saliva, designed to mimic the properties of normal human saliva, affects the mechanical properties of non dental glass fiber, including tensile strength. This research aims to determine the effect of immersion duration of non-dental glass fiber reinforced composite in artificial saliva on tensile strength.Method: The research method used is laboratory experimental analytic with a post-test only control group design. Samples are divided into three treatment groups with immersion in artificial saliva for three times (2, 7, and 14 days). Tensile strength is conducted using a Universal Testing Machine (UTM).Conclusion: The conclusion drawn from this research is that immersion duration in artificial saliva has a significant effect on tensile strength of non-dental glass fiber reinforced composite. The tensile strength of non-dental glass fiber reinforced composite after immersion for 14 days shows higher results compared to immersion for 7 and 2 days
EFFECT OF EXTRACT GEL GREEN TEA (Camelia Sinensis) ON NEUTROPHIL OF POST EXTRACORONAL BLEACHING RAT’S TEETH
No full textBackground: Free radicals produced by hydrogen peroxide after teeth bleaching which diffuse through the enamel and dentinal tubules can trigger inflammation of the pulp. To overcome this negative side effect, exogenous antioxidants have been proposed. This research was conducted to determine the effect of natural antioxidants, namely 5% and 10% green tea, which was applied on rat molars teeth that were bleached using 40% hydrogen peroxide. Methods: Fifteen molar teeth of male Wistar rats were bleached using 40% hydrogen peroxide on their occlusal surface. Furthermore, the control group (Group I) rinsed using warm distilled water, while in Group II and Group III, the teeth were rinsed using 5% and 10% green tea extract gel. Wistar rats were sacrificed on the fifth day after treatment, followed by hematoxylin-eosin staining. Histological examinations were observed under a light microscope using 400x magnification and the number of the neutrophil were counted. Result: One-way ANOVA test showed that there was a significant effect of green tea extract gel concentration on the number of neutrophils (p 0.05). Post hoc test results showed that there were significant differences in all pairs of groups compared (p 0.05). Conclusion: Group III which was treated using10% green tea extract gel has the lowest number of neutrophil compared to Group II which was treated using 5% green tea extract hel and the control group
The Effectiveness of Moringa Oleifera Lam Extract as an Oral Antimicrobial: a Systematic Literature Review
Get PDFBackground: The use of mouthwash to maintain oral hygiene has been carried out for many years. However, long-term use of mouthwash can cause side effects such as tooth discoloration, burning sensation and the death of normal oral flora. The use of herbal plant, such as Moringa oleifera lam has been studied as an alternative medicine because of its pharmacological properties and it is hoped to have minimum side effect. In this systematic literature review, we included the studies that have been investigate the antimicrobial capacity of Moringa oleifera lam as a basic knowledge to develop a preparation for oral hygiene maintenance.Method: A comprehensive literature search was performed utilizing four different electronic databases: PubMed, Scopus, Cochrane library, and EBSCO. Articles included in this study were published from 2013 to September 2023, with broad search criteria comprising MeSH-terms and free-text keywords Moringa Oleifera, Oral Antimicrobial, and Mouthwash.Result: At the beginning our search resulted in 17 articles, with 14 articles remaining after removal of duplication. We exclude five studies that do not meet the PICO criteria, resulting seven articles which included to this study.Conclusion: According to the literature review that has been carried out, Moringa oleifera lam has antimicrobial capacity which allows it to be used as a material in medicinal preparations. Further research, both in vitro and clinical research, will determine the effectiveness of Moringa oleifera lam in suppressing microorganisms
Viability of BHK-21 Fibroblast Cells Resulting from Synthesis of Collagen Powder (Thunnus albacares) with Variation of NaCl Concentration
Get PDFBackground: Collagen plays a crucial role in post-extraction wound healing. Thunnus albacares skin, a collagen source synthesized through the papain soluble collagen (PaSC) method with varying NaCl concentrations, is assessed for non-toxicity using the MTT assay on BHK-21 fibroblast cell cultures. This research is to determine cell viability resulting from synthesizing collagen powder from the skin of yellowfin tuna (Thunnus albacares) with varying NaCl concentrations using the papain soluble collagen (PaSC) method. Method: Collagen powder was synthesized by cleaning Thunnus albacares skin measuring 1x1 cm, soaking it in 0.1 M NaOH, hydrolyzing it with CH3COOH, extracting it with papain enzyme, and then dividing the samples into four groups: without NaCl, NaCl 0.9 M, NaCl 1.3 M, and NaCl 1.7 M. Samples were centrifuged (6000 rpm) and followed by freeze-drying. Cell viability was obtained by conducting a cytotoxicity test using the MTT Assay method on BHK-21 fibroblast cells. Result: The percentage of cell viability in groups K, P1, P2, P3, and P4 were 100%, 10.708%, 113.750%, 107.833%, and 105.958%, respectively. The Kruskall-Wallis test yielded a significance value 0.000, indicating a significant difference (p<0,005). The Mann-Whitney test confirmed significant differences between groups. Conclusion: Collagen powder from Thunnus albacares skin with NaCl concentrations of 0.9 M, 1.3 M, and 1.7 M showed no toxic effects, while the group without NaCl showed toxic effects. Collagen powder with a NaCl concentration of 1.7 M yielded ideal results and showed no toxic effects
Potential Natural Antibacterial Agent for P. gingivalis Periodontitis Infection: A Comprehensive Review of Source, Structure and Mechanism actions
Get PDFThe pathogenic bacteria P. gingivalis grows in the oral cavity. This bacterium could attack immune system which lead to inflammation of most tissues. P. gingivalis can cause a variety of serious and dangerous condition such as periodontitis, Alzheimer, rheumatoid arthritis, diabetes, and pneumonia. Antibiotics have been used for years as a treatment against this bacterium, like metronidazole, amoxicillin, and clindamycin, however, P. gingivalis is reported to be resistant to these antibiotics, thus exploration to discover alternatives has been demanded. Natural product compounds are known to have antibacterial activity and cause fewer side effects. Turmeric, eucalyptus, and several other plants have been reported to have antibacterial activity against P. gingivalis with a MIC of 1g/mL from an ethyl acetate leaf extract of eucalyptus. Decent antibacterial activity could be used as a reference to discover new drugs as alternatives against P. gingivali