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Fermentation of pineapple juice with Lactiplantibacillus plantarum subsp. plantarum Dad-13: Sensory and microbiological characteristics
Among the varieties of pineapples, honey pineapple is suitable to be processed as a probiotic beverage. The study aimed to evaluate the honey pineapple juice as a growth medium for Lactiplantibacillus plantarum subsp. plantarum Dad-13 probiotic strain. The pineapple juice was fermented by adding a starter culture of L. plantarum Dad-13. The fermentation time (0, 4, 8, 12, 16, 20, and 24 h) was used as a variable. The number of L. plantarum Dad-13 cells increased significantly after fermentation (P < 0.05). In acidic pineapple juice L. plantarum Dad-13 can still grow more than 2 log cycles. After 16 h, the results showed the best characteristics with cell count (8.86 log CFU·mL�1), pH (3.52), and titratable acidity (0.59). To balance the sour taste, the 8 sucrose addition was the most preferred by the panellists (n = 67), with a 5.74 (slightly like) overall acceptance score. After 42 days, there was no significant decrease in cell viability. The number of cells on day 42 was 8.81 log CFU·mL�1 with significant changes in pH and titratable acidity. The study showed that honey pineapple juice is a suitable growth medium for L. plantarum Dad-13. © The authors. This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
Cold Stress Response Genes of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Lantiplantibacillus plantarum subsp. plantarum Mut-7 Support the Ability to Survive in Low-Temperature Conditions
Probiotics are widely consumed in various food matrices to provide health benefits to the host. The viability of probiotic cells is influenced by several factors, including exposure to high temperatures during the production process and low temperatures during storage. In this study, we report the response to cold stress of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Mut-7 after 24 h of storage at 4°C and-20�. The cell number of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Mut-7 in low-temperature condition had no significant differences than their initial number: 11.88 log CFU/ml and 11.62 log CFU/ml at 4°C; 11.51 log CFU/ml and 11.47 log CFU/ml at-20°C for Mut-3 and Mut-7 respectively. The results indicated the survival capacity of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Mut-7 at low temperatures. The genes encoding cold shock proteins for the response to cold stress were evaluated by genome sequencing. The CspA/CspC genes of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Mut-7 possibly play a role in maintaining cell resistance at low temperatures, since the genes products predicted to have conserved motifs in the RNA binding protein (RNP)-1 and RNP-2 responsible for cold response stress which are similar to those in other bacteria. © 2023, Bogor Agricultural University. All rights reserved
Pengukuran Output Harian Linear Accelerator di Instalasi Kanker Terpadu RSUP Dr. Sardjito
Kanker adalah salah satu penyakit dengan kasus terbanyak dan tingkat mortalitas yang tinggi di Indonesia. Penyembuhan penyakit kanker sendiri dibedakan menjadi 3, yaitu operasi, kemoterapi, dan radioterapi. Salah satu teknologi radioterapi yang digunakan di RSUP Dr. Sardjito adalah radioterapi eksternal dengan menggunakan Linear Accelerator (LINAC) yang akan menembakkan partikel foton ke tubuh pasien. Pada Kerja Praktik ini, salah satu kegiatan yang dilakukan adalah pengukuran keluaran LINAC setiap harinya sebelum jadwal terapi pasien dimulai. Kegiatan ini juga disebut dengan Quality Assurance (QA). Pengukuran dilakukan
dengan pendamingan fisika medis yang bertugas. Hasil pengukuran harian ini dibandingkan dengan referensi dari pengukuran bulanan dengan perbedaan tidak boleh melebihi 3%. Hasil pengukuran menunjukkan setiap harinya modalitas masih dalam kondisi sangat baik untuk digunakan untuk terapi kanker bagi pasien. Dalam Kerja Praktik ini, dipelajari juga berbagai ilmu baru, seperti perencanaan radioterapi, etika
profesi, dan komunikasi antar profesi
Pengukuran Paparan Ruangan, Alat Pelindung Diri, serta Aktivitas Limbah Pasien sebagai Penerapan Prinsip Proteksi Radiasi di Instalasi Kedokteran Nuklir RSUP Dr. Sardjito
Kedokteran Nuklir adalah bidang ilmu kesehatan yang menggunakan
sumber radioaktif terbuka untuk keperluan diagnostik dan terapi pasien, sehingga
perlu dilakukan pemeriksaan kontaminasi pada ruangan dan alat pelindung diri
(APD) secara harian.
Pengukuran paparan lingkungan dilakukan pada tiap ruang di instalasi
Kedokteran Nuklir menggunakan surveymeter inspector. Hasil pengukuran
menunjukkan tidak ada ruangan untuk masyarakat umum yang rerata paparannya
melebihi 0,5 uSv/jam, hal ini telah memenuhi standar untuk paparan masyarakat
umum berdasarkan Perka Bapeten No. 4 tahun 2013.
Pemeriksaan kontaminasi APD dilakukan dengan mengukur APD dengan
surveymeter dan membandingkannya dengan rerata hasil pengukuran paparan
ruangan +
Pengukuran Dosis Injeksi Radiofarmaka pada Pasien Bone Scan di RSPAD Gatot Soebroto Jakarta
Di Indonesia, kanker merupakan salah satu penyakit penyebab kematian
terbesar dengan penderitanya sebesar kisaran 70% dan terlebihnya lagi ditemukan
dalam keadaan stadium yang sudah lanjut. Rumah Sakit Pusat Angkatan Darat
Gatot Soebroto merupakan rumah sakit kepresidenan yang menjadi salah satu
instansi pelayanan kesehatan dengan teknologi perawatan untuk pasien kanker yang
cukup lengkap dan memiliki fasilitas Kedokteran Nuklir dan Radioterapi. Instalasi
Kedokteran Nuklir dan Radioterapi merupakan instalasi yang penting karena dapat
melakukan diagnosis penyakit tumor/kanker ketika masih dalam stadium awal
sehingga dapat mengantisipasi penyakit kanker tersebut. Instalasi Kedokteran
Nuklir sendiri memiliki kelebihan yang mampu mencapai target organ spesifik
tertentu.
Pada Kerja Praktik di instansi Kedokteran Nuklir dan Radioterapi ini,
banyak dilakukan berbagai pembelajaran terkait dengan bidang Fisikawan Medis
seperti proteksi radiasi, dekontaminasi sumber radioaktif terbuka, pengelusian
radiofarmaka, kendali mutu radiofaramaka (Quality Control dan Quality
Assurance), SPECT, LINAC, dann TPS. Selain pembelajaran terkait hardskill,
penulis juga mendapatkan pengalaman berharga untuk dapat bekerja dibawah
naungan tentara, dan juga keterampilan terkait softskill seperti etika dan sopan
santun terhadap berkomunikasi.
Dalam menjalani KP hingga KP berakhir, didapatkannya pembelajaran
yang sangat berguna bagi penulis dan rekan rekannya seperti ilmu pengetahuan
yang hanya dapat diperoleh dari lapangan, etika dalam bekerja, selalu senyum sapa
salam, dan menjalin silaturahmi terhadap pekerja rumah sakit.
Kata Kunci: Kedokteran Nuklir, Radioterapi, SPECT, LINA
Network analysis reveals overlapping roles of stakeholders related to seagrass-data provisioning in Indonesia
Seagrass data support conservation areas, carbon budget, natural resource assessment, and other national interests in Indonesia. However, to what extent seagrass-related data are available and how the data are managed are still unknown. In this study, we assess the status of data availability and the roles of stakeholders in seagrass-related data provisioning at a national level in Indonesia. Data were collected in August and September 2022 through Focus Group Discussion (FGD) and a questionnaire. The participants comprised representatives from the central governments (planning, ministerial, research, geospatial agencies), local governments, universities, and non-governmental organizations (NGOs) working in the fields related to the coastal ecosystem. Stakeholders’ roles were analyzed using Stakeholders’ Analysis (SA), while their relationships were examined with Social Network Analysis (SNA). The results showed that the seagrass areal extent (61.7) was the most crucial data needed, followed by the percent cover (12.77). Using seagrass data as coastal management was the highest priority among stakeholders. There were overlapping roles between stakeholders related to seagrass data, as producers or users, due to their urgent needs. Almost all stakeholders were key players, and there was a lack of coordination among them relating to seagrass data provisioning. There was an urgent need to strengthen seagrass data governance. The results of this work form an important pathway for Indonesia to develop approaches to map the seagrass areal extent and share knowledge. © 2023 Elsevier Lt
Integrating gender mainstreaming in disaster risk reduction through providing geospatial information to create community resilience in Muntuk Village, Bantul Regency
Muntuk Village, Bantul Regency has a high multi-risk disaster potential, in the form of earthquakes and landslides. This is due to the location of Muntuk Village which is in the Bukit Batur Agung formation and is directly connected to the Opak Fault. The implications of these threats result in high disaster vulnerability. The focus of this research is to examine aspects of social vulnerability with the main subject being women, especially since the number of victims of the 2006 Bantul earthquake was dominated by women. This research seeks to integrate the development of Prima Village to increase the resilience of women's communities. The method used is a concurrent mixed method using questionnaires, interviews, and observations, as well as mapping data processing. The results of the analysis produced several outputs, including Sex Ratio Map, Economic Resilience Index Map, Prima Village Feasibility Standard Map, Landslide Prone Area Map, Logistics Warehouse Coverage Effectiveness Map, Logistics Warehouse Development Plan Map, and policy recommendations. It is hoped that this output can be used as input in designing regional disaster planning policies, both pre-disaster, during a disaster, and post-disaster, especially for women to improve community empowerment programs to achieve the goals of Prima Village. © 2023 EDP Sciences. All rights reserved
The Growth of Urban Heat Island Effect Monitored in a Rapidly Developing City of Lombok Island
The study aims to monitor urban development around Mataram City and the correlation between Urban Heat Island. Also, analyze the vegetation and built-up changes surrounding Mataram City with Land Surface Temperature (LST) growth. The boundary of the study location is a 25-kilometer radius from the Mataram City centroid. The data used in this research is Landsat 8 Surface Reflectance for the period June-September in 2013, 2019 and 2023 obtained from Google Earth Engine. The Random Forest method is used to obtain land use classification with urban, rice fields, dry land, water bodies, and vegetation. NDBI values are processed from Shortwave infrared (SWIR) and Near-infrared (NIR), NDVI from Near-infrared (NIR) and Red, and LST from thermal bands. The data processing results indicate that Mataram City and its neighboring areas are showing growth, marked by rising NDBI and LST values. This growth is characterized by an expansion of built-up areas to the north, east, and south especially rings 4 and 5, simultaneously by higher NDBI values. Additionally, there is a decrease in vegetation cover, particularly in lowland areas within these rings, leading to a decline in NDVI values. Areas with increased NDBI values also show an increase in LST. © 2023 EDP Sciences. All rights reserved
Coastal sedimentation and topographic changes in the Mataram Plain, Lombok (Indonesia) following the 1257 CE eruption of Samalas volcano
The 1257 CE eruption of Samalas volcano, Indonesia, buried the entire island of Lombok beneath various thicknesses of pyroclastic material during this Volcanic Explosivity Index (VEI) 7 event. This study aims to reconstruct the paleo-topography of western Lombok before the Samalas eruption in 1257 CE and analyse the sedimentation processes that led to its landscape evolution over the last 700 years. Stratigraphic data were collected from various surveys, such as outcrops, coring, drilling, hand auger and wells observation. Electrical resistivity tomography (ERT) measurements were performed to complement the stratigraphic data. A combination of stratigraphy, ERT and topographic data from various sources (topographic map, Digital Elevation Model Nasional DEMNAS and Real Time Kinematic RTK measurement) is employed to determine the depth of the paleo-surface. Topographic modelling was performed by subtracting the delta-DEM (DDEM) from the current-DEM (CDEM). The result of this operation is a paleo-DEM, which is used to reconstruct the paleo-hydrographic features such as shoreline positions and river channels. Modelling results demonstrate that the relief has not been significantly modified, except in lowland areas, which is the sediment accumulation zone. River channels have experienced minor changes, except for the location of the river mouth and the degree of meandering. Significant changes occurred at the shoreline that has prograded by approximately 1.6 km during the last 700 years. A schematic model is built to illustrate the evolution processes of the study area, consisting of the pre-eruption condition, the immediate post-eruption condition and development of the current condition. This model further develops the previously proposed model with a higher resolution and simultaneously revises the estimated boundary of the pyroclastic density current (PDC) deposit from previous work. © 2023 John Wiley & Sons Ltd
Identification of urban form in the Bandung metropolitan area
Urban forms can transform from monocentric to polycentric structures. Identification of urban forms can be done with various methodologies such as using commuter flows and employment density. This research aimed to identify the location of the employment sub-centre and find out urban forms based on commuting patterns in the Bandung metropolitan area. The method used is quantitative analysis with locally weighted regression (LWR) and general functional polycentricity (Pgf) index. The results showed that traditional commuting still dominates with Bandung city as the main destination for commuting and Bandung Regency as the largest commuting sending area. There are six sub-districts that become employment sub-centres that have spread across three regencies/cities. However, the polycentricity index produces a value of 0.028 indicating that urban forms still tend to be monocentric. This condition indicates that although there is a sub-centre of employment in the suburban area, the flow of population mobility still tends to lead to Bandung city as a metropolitan center. Thus, the urban form in the Bandung metropolitan area still tends to be monocentric or weakly polycentric. © 2023, The Author(s), under exclusive licence to Springer Nature B.V