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Identification of early flowering mutant gene in Phalaenopsis amabilis (L.) Blume for sgRNA construction in CRISPR/Cas9 genome editing system
No full textPhalaenopsis amabilis (L.) Blume commonly called Moth Orchid (Orchidaceae) is a natural orchid species designated as the National Flower of Indonesia for its beautiful flower shape and long-lasting flowering period. Basically, P. amabilis has a long vegetative phase that cause late flowering, about 2 to 3 years for flowering, hence a method to shorten vegetative period is desired. The latest technological approach that can be used to accelerate flowering of P. amabilis is the CRISPR/Cas9 genome editing method to inactivate the GAI (Gibberellic Acid Insensitive) gene as a mutant gene that can accelerate the regulation of FLOWERING TIME (FT) genes flowering biosynthesis pathway. The approach that needs to be taken is to silence the GAI gene with a knockout system which begins with identifying and characterizing the GAI target gene in the P. amabilis which will be used as a single guide RNA. CRISPR/Cas9 mediated knockout efficiency is highly dependent on the properties of the sgRNA used. SgRNA consists of a target sequence, determining its specificity performance. We executed phylogenetic clustering for the PaGAI protein with closely related orchid species such as Dendrobium capra, Dendrobium cultivars and Cymbidium sinensis. SWISS-Model as tool webserver for protein structure homology modeling. Results show that P. amabilis has a specific domain with the occurrence of point mutations in the two conservative domains. Therefore, a single guide RNA reconstruction needs to be implemented. © 2024, Instituto Internacional de Ecologia. All rights reserved
Survei kebutuhan sumber informasi Perpustakaan Universitas Gadjah Mada, tahun 2024
No full textSurvei kebutuhan sumber informasi Perpustakaan UGM Tahun 2024 difokuskan pada kebutuhan pemustaka terhadap sumber informasi cetak dan sumber informasi elektronik. Tujuan dilakukannya survei adalah sebagai bahan pertimbangan dalam mengambil kebijakan pengadaan koleksi melalui pembelian sumber informasi cetak dan elektronik.Sebagai salah satu perpustakaan dari universitas riset terbesar di Indonesia, Perpustakaan Universitas Gadjah Mada (Perpustakaan UGM) berperan aktif dalam kemajuan ilmu pengetahuan dan penelitian, demi terwujudnya pembangunan berkelanjutan. Peranan tersebut diwujudkan melalui ketersediaan sumber-sumber informasi baik tercetak maupun elektronik yang dapat dimanfaatkan oleh sivitas UGM dalam proses pembelajaran dan penelitian. Ketersediaan sumber-sumber informasi tersebut meliputi buku cetak, terbitan berkala cetak, buku electronik (EBooks), database elektronik, dan jurnal elektronik (eJournal). Jumlah responden yang terlibat dalam survei sebanyak 385 pemustaka. Hasil survei menunjukkan bahwa sebanyak 300 responden atau 77,92% berpendapat bahwa ketersediaan koleksi di Perpustakaan UGM sudah mencukupi kebutuhan sumber literatur mereka. Responden menyatakan bahwa mereka sangat membutuhkan sumber informasi jenis buku cetak dan buku elektronik, terbitan berkala cetak dan elektronik, dan basis data dilanggan Perpustakaan UGM. Rekomendasi survei memfokuskan untuk berlangganan sumber informasi elektronik atau database sesuai hasil survei, berlangganan terbitan berkala sesuai hasil survei, dan menyusun daftar usulan pengadaan untuk koleksi cetak sesuai usulan responden
Leaders' Sensemaking in Communicating Organizational Change
No full textOrganizational change management is highly dependent on leaders. However, more research is needed on how leaders react and interact with employees throughout organizational change, especially in the public sector. Current research commonly employs generalized beliefs, such as leadership behaviors, which frequently contradict each other, to explain leadership communication during the organizational transition. Therefore, this study aims to explore how leadership communication was conducted in organizational transformation. This research used qualitative, interpretive, and case study approaches to examine how two public organizations prepared for and integrated into a brand-new government agency. The sensemaking viewpoint was utilized to investigate leadership communication in managing organizational change. Twenty-seven participants from various organizational levels were interviewed to obtain a holistic picture of leadership communication. This article contributes to our understanding of leadership communication by illustrating how leaders integrate bracketed information from various relevant sources, select statements, and reconstruct change mindsets through communication to navigate the management change discourse within an organization. Additionally, communication by leaders implies the existence of environmental data intended for use in the development of growth-promoting policies. This study has a dichotomy between change-orientated communication and resistance to change leadership communication. This polarization suggests that a shift in the policy's professional emphasis on growth, eliminating existing tension, and constructing new institutions will be necessary. Communication among leaders is based on different organizational-level methods to gather and interpret information, which considers the organization's structure and work culture. © 2023 The authors
Effects of caffeine intake and performance pressure on working memory
No full textPerformance pressure and caffeine consumption, a common combination in daily life, have both been shown to affect cognitive performance. However, previous research has not fully elucidated the extent to which the effects of caffeine and performance pressure impact cognitive function, especially working memory. This study aims to examine the possibility that caffeine can enhance working memory performance under pressure. A total of 61 participants aged 18 to 32 participated, divided into four groups. Experiment-based data collection was conducted with a single-blind design. Working memory was measured by Modular Arithmetic Tasks with the OpenSesame program. All participants were asked to perform arithmetic tasks and arousal levels were measured using the Galvanic Skin Response (GSR). The findings revealed no evidence of an interaction effect of caffeine intake and performance pressure on working memory (F = .632, p = .431, ηp2 = .012). Given the prevalence of caffeine intake prior to facing high-pressure situations, the consumption of a cup of coffee does not improve cognitive performance as many would expect. However, caffeine intake had a stabilizing effect on the skin conductance response values during performance under pressure. Clinical psychologists can use a daily dose of caffeine as an alternative intervention or preventative measure to help patients reduce performance pressure-related anxiety. Copyright © 2023 Psikohumaniora: Jurnal Penelitian Psikologi
Improved Dynamic Threshold Timer for Emergency Vehicle Preemption Exit Strategy
No full textThis paper examines the Dynamic Threshold Timer (DTT) as an exit strategy for Emergency Vehicle Preemption (EVP), a critical service that prioritizes emergency vehicles' passage through traffic by pre-empting traffic signals. However, the use of EVP can lead to traffic congestion and delays for other vehicles. To address this issue, an Exit Strategy can be used to optimize traffic signal timing and improve traffic flow, thereby mitigating the negative impacts of EVP on other vehicles. The study focuses on a case study of one such strategy: Dynamic Threshold Timer, which was enhanced and evaluated for its effectiveness in reducing waiting time. The results indicate that the improvisation of DTT into the Dynamic Exit Phase can significantly reduce the average waiting time by 18% across all traffic flows saturation level. In contrast, the Fixed Phase does not reduce congestion but rather increases it by 10%, compared to a traffic light system without the Exit Strategy. Furthermore, the study reveals that applying DTT with an exit to the South Phase yields the most effective results, reducing waiting time by 20%. The findings from this research underscore the potential of utilizing DTT with exit strategies for the Dynamic Phase to improve traffic flow and mitigate delays. The implications of this study are significant for managing smart city transportation systems and enhancing emergency service provision. © 2023 IEEE
Analyzing Recent Seismic Activity of the Opak Fault System in Central Java, Indonesia, From 2009 To 2021
No full textA strong earthquake occurred on May 26, 2006, at 22:53:58 UTC, with a magnitude of Mw6.4. The shock was felt with an intensity of VI-VII MMI around Yogyakarta, Indonesia. The earthquake was presumably caused by the movement of the Opak Fault. Following the strong earthquake, seismic activity along the fault has remained high to this day. In order to explain the progression of seismic activity and understand the mechanism of the Opak fault, we conducted catalog relocation, focal mechanism inversion, and statistical analysis of the earthquake events from 2009-2021. The events were relocated using the Double Difference Method. To improve the accuracy of the focal mechanism inversion, we updated the 1-D velocity model from Crust 1.0 to a local velocity model. We inverted the mechanism of earthquakes with a magnitude of Mâ�¥3.0. The results indicate that the recent hypocenters are clustered in the southeastern part of the Opak Fault. This cluster is located within the rupture zone of the Mw6.4 2006 mainshock, providing further evidence that post-earthquake deformation from 2006 is still ongoing and primarily involves left-lateral oblique-slip faulting. The mechanism results are consistent with the observable morphological contrast on the surface. Cross-section plots of seismicity and dip angle, perpendicular to the mainshock strike, reveal a flower structure pattern, indicating a complex mechanism. The fault system is believed to be in the interseismic period, supported by the low b-value. The suspicion is further strengthened by an increase in microseismic activity and a decrease in M\textgreater3.0. This evidence suggests that the Opak Fault is currently experiencing strain accumulation. © Int. J. of GEOMATE All rights reserved, including making copies, unless permission is obtained from the copyright proprietors
Effect of chemical composition on hot cracking susceptibility (HCS) using various hot cracking criteria
No full textThe paper aims to evaluate the effect of chemical composition on the Hot Cracking Susceptibility (HCS) using mechanical and non-mechanical hot cracking criteria during solidification. The criteria were SKK as a mechanical criterion. Feurer, Clyne Davis, and Katgerman as non-mechanical criteria. The criteria were implemented at various parameters to evaluate their abilities in the hot cracking susceptibility (HCS) prediction at varied chemical composition. In this study, The Mg content was varied in Al9Zn (1, 1.5, 2, 2.5 %wt.) Mg2Cu alloys and Cu content in Al9Zn2Mg (1, 1.5, 2, 2.5 %wt.) Cu alloys. The validation of the result is also conducted by comparing with the experimental data. Based on Feurer criterion, The hot cracking initiates at lower temperature and at higher critical rate of feeding and shrinkage with Cu content, and the hot cracking initiates at higher temperature with Mg content, and it initiates at higher critical rate of feeding and shrinkage from 1 up to 1.5 of Mg, and the critical rate of feeding and shrinkage remains constant from 1.5 up to 2.5 of Mg. Based on Clyne & Davies, the HCS decreases with Cu content from 1 up to 2 of Cu, and it increases from 2 up to 2.5 of Cu. The HCS decreases with Mg content from 1 up to 2 of Mg, and it remains constant from 2 up to 2.5 of Mg. Based on Katgerman criterion, the HCS decreases with Cu content from 1 up to 1.5 of Cu, it increases from 1.5 up to 2 of Cu, and it decreases from 2 up to 2.5 of Cu. The HCS decreases sequentially with Mg content. Based on SKK criterion, the HCS curves shift to the right with Cu content which means that the hot cracking initiates at lower temperature, and the HCS curves shift to the left with Mg content which means that the hot cracking initiates at higher temperature with Mg content. The Feurer, Clyne & Davies, and some specific range for SKK criteria are in agreement for the effect of Cu content on HCS of alloys, and Katgerman and some specific range for Clyne&Davies criteria are in agreement for the effect of Mg content on HCS of alloys
A decision-making model for supporting selection of green building materials
No full textThe manufacture and use of building materials result in numerous environmental concerns in the construction industry. Green building materials (GBMs) are environmentally friendly compared to their traditional counterparts. However, identifying the criteria for selecting GBMs and assessing their sustainability have been challenging. In this study, the Analytic Hierarchy Process (AHP) technique was used to decide between selecting steel building structure and reinforced concrete (RC) building structure as an alternative. In the first phase, the criteria for selecting GBMs were identified through literature review, experts' opinions, and questionnaire survey. In the second phase, the purposive sampling technique was used to select experts and professionals in materials selection to compare the different GBMs criteria and also, to compare and weight the provided alternatives. The findings of this study identified that 29 criteria were ranked using a scatter plot of means and standard deviation scores. Besides, the results indicated that RC building structure, with a weight value of 0.5225, was a better alternative to steel building structure. Additionally, the results show that the environmental and economic criteria are more important than the social criteria. This study, therefore, developed a decision-making model for helping stakeholders to select materials required to achieve sustainable buildings
Failure in power plant system related to mitigations and economic analysis; A study case from steam power plant in Suralaya, Indonesia
No full textThe failure mitigation and economic analysis related to the damage of the boiler tube in the power plant system were evaluated in the present study. The research was conducted at Indonesia's Suralaya power plant (PP). The boiler was built in 2011 and experienced a boiler tube failure after operating for less than 5 years. This condition is not common since boilers generally can be used with a lifetime of approximately 30 years. In the present study, different analyses were used i.e., macro-fractography, metallography inspection, hardness test, SEM/XRD analysis, and hydro test. These tests investigated the failure modes, evaluated the tube's mechanical properties, and assessed the boiler water feed's quality. The results showed that the damage is caused by the below-standard quality materials and is triggered by the poor quality of boiler feed water and exhibit corrosion. It is proven that the high value of sodium, conductivity, and silica content causes corrosion of the inner tube, which came out from methane gas. It is also shown that the damage drastically decreased the quality of the water wall tube strength, indicated by the HRV value from 179 HRV to 112 HRV. The analysis was a brief study that can be used for manufacturers and industries that use the boiler to check the material quality and the detailed specification. Moreover, the below-standard materials have proven to cause economic loss and burden many sectors' maintenance and operation costs
Review of Financial Penalties Implementation on Non-Compliance With Road Performance Indicators
No full textIndonesia's national road preservation system, mainly routine road maintenance, was initially carried out independently by the project manager as a road section manager. This maintenance has been transformed into a "long segment" scheme since 2016. The contractor is subject to financial penalties in the form of withholding payments for each failure to meet the road performance indicators within the specified repair response times. The government, specifically the project manager, has not adequately applied the current financial penalties for late fulfillment of road performance indicators in Indonesia. The implementation of financial penalties that is not adequate is inversely proportional to the number of public complaints related to road performance, which represents the impact of road performance that does not meet the criteria. This study recommends developing a comprehensive policy for penalties related to the late fulfillment of road performance indicators. The policy should consider multiple indicators and components and adjust the coefficient of each component based on the severity of the condition. In addition, this study advises revising the formulation of financial penalties using relevant data, such as average daily traffic volume or public complaints