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Health promoting potential of herbal teas and tinctures from <i>Artemisia campestris</i> subsp. <i>maritima</i>: from traditional remedies to prospective products
Get PDFThis work explored the biotechnological potential of the medicinal halophyte Artemisia campestris subsp. maritima (dune wormwood) as a source of health promoting commodities. For that purpose, infusions, decoctions and tinctures were prepared from roots and aerial-organs and evaluated for in vitro antioxidant, anti-diabetic and tyrosinase-inhibitory potential, and also for polyphenolic and mineral contents and toxicity. The dune wormwood extracts had high polyphenolic content and several phenolics were identified by ultra-high performance liquid chromatography–photodiode array–mass-spectrometry (UHPLC-PDA-MS). The main compounds were quinic, chlorogenic and caffeic acids, coumarin sulfates and dicaffeoylquinic acids; several of the identified phytoconstituents are here firstly reported in this A. campestris subspecies. Results obtained with this plant’s extracts point to nutritional applications as mineral supplementary source, safe for human consumption, as suggested by the moderate to low toxicity of the extracts towards mammalian cell lines. The dune wormwood extracts had in general high antioxidant activity and also the capacity to inhibit α-glucosidase and tyrosinase. In summary, dune wormwood extracts are a significant source of polyphenolic and mineral constituents, antioxidants and α-glucosidase and tyrosinase inhibitors, and thus, relevant for different commercial segments like the pharmaceutical, cosmetic and/or food industries
GC content of early metazoan genes and its impact on gene expression levels in mammalian cell lines
Get PDFWith the genomes available for many animal clades, including the early-branching metazoans, one can readily study the functional conservation of genes across a diversity of animal lineages. Ectopic expression of an animal protein in, for instance, a mammalian cell line is a generally used strategy in structure–function analysis. However, this might turn out to be problematic in case of distantly related species. Here we analyzed the GC content of the coding sequences of basal animals and show its impact on gene expression levels in human cell lines, and, importantly, how this expression efficiency can be improved. Optimization of the GC3 content in the coding sequences of cadherin, alpha-catenin, and paracaspase of Trichoplax adhaerens dramatically increased the expression of these basal animal genes in human cell lines
Frame-insensitive expression cloning of fluorescent protein from <i>Scolionema suvaense</i>
Get PDFExpression cloning from cDNA is an important technique for acquiring genes encoding novel fluorescent proteins. However, the probability of in-frame cDNA insertion following the first start codon of the vector is normally only 1/3, which is a cause of low cloning efficiency. To overcome this issue, we developed a new expression plasmid vector, pRSET-TriEX, in which transcriptional slippage was induced by introducing a DNA sequence of (dT)14 next to the first start codon of pRSET. The effectiveness of frame-insensitive cloning was validated by inserting the gene encoding eGFP with all three possible frames to the vector. After transformation with one of these plasmids, E. coli cells expressed eGFP with no significant difference in the expression level. The pRSET-TriEX vector was then used for expression cloning of a novel fluorescent protein from Scolionema suvaense. We screened 3658 E. coli colonies transformed with pRSET-TriEX containing Scolionema suvaense cDNA, and found one colony expressing a novel green fluorescent protein, ScSuFP. The highest score in protein sequence similarity was 42% with the chain c of multi-domain green fluorescent protein like protein “ember” from Anthoathecata sp. Variations in the N- and/or C-terminal sequence of ScSuFP compared to other fluorescent proteins indicate that the expression cloning, rather than the sequence similarity-based methods, was crucial for acquiring the gene encoding ScSuFP. The absorption maximum was at 498 nm, with an extinction efficiency of 1.17 × 105 M−1·cm−1. The emission maximum was at 511 nm and the fluorescence quantum yield was determined to be 0.6. Pseudo-native gel electrophoresis showed that the protein forms obligatory homodimers
Deep-sea benthic communities and oxygen fluxes in the Arctic Fram Strait controlled by sea-ice cover and water depth
Get PDFArctic Ocean surface sea-ice conditions are linked with the deep sea benthic oxygen fluxes via a cascade of interdependencies across ecosystem components such as primary production, food supply, activity of the benthic community, and their functions. Additionally, each ecosystem component is influenced by abiotic factors such as light availability, temperature, water depth, and grain size structure. In this study, we investigated the coupling between surface sea-ice conditions and deep-sea benthic remineralization processes through a cascade of interdependencies in the Fram Strait. We measured sea-ice concentrations, a variety of different sediment characteristics, benthic community parameters, and oxygen fluxes at 12 stations of the LTER HAUSGARTEN observatory, Fram Strait, at water depths of 275–2500m. Our investigations reveal that the Fram Strait is bisected into two long-lasting and stable regions: (i) a permanently and highly sea-ice-covered area and (ii) a seasonally and low sea-ice-covered area. Within the Fram Strait ecosystem, sea-ice concentration and water depth are two independent abiotic factors, controlling the deep-sea benthos. Sea-ice concentration correlated with the available food and water depth with the oxygen flux. In addition, both abiotic factors sea-ice concentration and water depth correlate with the macrofauna biomass. However, at water depths >1500m the influence of the surface sea-ice cover is minimal with water depth becoming more dominant. Benthic remineralization across the Fram Strait on average is ∼ 1mmol Cm−2d−1. Our data indicate that the portion of newly produced carbon that is remineralized by the benthos is 5% in the seasonally low sea-ice-covered eastern part of Fram Strait but can be 14% in the permanently high sea-ice-covered western part of Fram Strait. Here, by comparing a permanently sea-ice-covered area with a seasonally sea-ice-covered area, we discuss a potential scenario for the deep-sea benthic ecosystem in the future Arctic Ocean, in which an increased surface primary production may lead to increasing benthic remineralization at water depths <1500m
Spatial variability of biogeochemistry in shallow coastal benthic communities of Potter Cove (Antarctica) and the impact of a melting glacier
Get PDFMeasurements of biogeochemical fluxes at the sediment–water interface are essential to investigate organic matter mineralization processes but are rarely performed in shallow coastal areas of the Antarctic. We investigated biogeochemical fluxes across the sediment–water interface in Potter Cove (King George Island/Isla 25 de Mayo) at water depths between 6–9 m. Total fluxes of oxygen and inorganic nutrients were quantified in situ. Diffusive oxygen fluxes were also quantified in situ, while diffusive inorganic nutrient fluxes were calculated from pore water profiles. Biogenic sediment compounds (concentration of pigments, total organic and inorganic carbon and total nitrogen), and benthic prokaryotic, meio-, and macrofauna density and biomass were determined along with abiotic parameters (sediment granulometry and porosity). The measurements were performed at three locations in Potter Cove, which differ in terms of sedimentary influence due to glacial melt. In this study, we aim to assess secondary effects of glacial melting such as ice scouring and particle release on the benthic community and the biogeochemical cycles they mediate. Furthermore, we discuss small-scale spatial variability of biogeochemical fluxes in shallow water depth and the required food supply to cover the carbon demand of Potter Cove’s shallow benthic communities. We found enhanced mineralization in soft sediments at one location intermediately affected by glacial melt-related effects, while a reduced mineralization was observed at a location influenced by glacial melting. The benthic macrofauna assemblage constituted the major benthic carbon stock (>87% of total benthic biomass) and was responsible for most benthic organic matter mineralization. However, biomass of the dominant Antarctic bivalve Laternula elliptica, which contributed 39–69% to the total macrofauna biomass, increased with enhanced glacial melt-related influence. This is contrary to the pattern observed for the remaining macrofauna. Our results further indicated that pelagic primary production is able to fully supply Potter Cove’s benthic carbon demand. Therefore, Potter Cove seems to be an autotrophic ecosystem in the summer season
Sensitivity of the current Antarctic surface mass balance to sea surface conditions using MAR
Get PDFEstimates for the recent period and projections of the Antarctic surface mass balance (SMB) often rely on high-resolution polar-oriented regional climate models (RCMs). However, RCMs require large-scale boundary forcing fields prescribed by reanalyses or general circulation models (GCMs). Since the recent variability of sea surface conditions (SSCs, namely sea ice concentration, SIC, and sea surface temperature, SST) over the Southern Ocean is not reproduced by most GCMs from the 5th phase of the Coupled Model Intercomparison Project (CMIP5), RCMs are then subject to potential biases. We investigate here the direct sensitivity of the Antarctic SMB to SSC perturbations around the Antarctic. With the RCM "Modele Atmospherique Regional" (MAR), different sensitivity experiments are performed over 1979-2015 by modifying the ERA-Interim SSCs with (i) homogeneous perturbations and (ii) mean anomalies estimated from all CMIP5 models and two extreme ones, while atmospheric lateral boundary conditions remained unchanged. Results show increased (decreased) precipitation due to perturbations inducing warmer, i.e. higher SST and lower SIC (colder, i.e. lower SST and higher SIC), SSCs than ERA-Interim, significantly affecting the SMB of coastal areas, as precipitation is mainly related to cyclones that do not penetrate far into the continent. At the continental scale, significant SMB anomalies (i.e greater than the interannual variability) are found for the largest combined SST/SIC perturbations. This is notably due to moisture anomalies above the ocean, reaching sufficiently high atmospheric levels to influence accumulation rates further inland. Sensitivity experiments with warmer SSCs based on the CMIP5 biases reveal integrated SMB anomalies (+5 % to +13 %) over the present climate (1979-2015) in the lower range of the SMB increase projected for the end of the 21st century
Rising oceans guaranteed: Arctic land ice loss and sea level rise
Get PDFPurpose of Review This paper reviews sea level contributions from land ice across the Arctic, including Greenland. We summarize ice loss measurement methods, ice loss mechanisms, and recent observations and projections, and highlight research advances over the last 3-5 years and remaining scientific challenges. Recent Findings Mass loss across the Arctic began to accelerate during the late twentieth century, with projections of continued loss across all future greenhouse gas emission scenarios. Recent research has improved knowledge of ice hydrology and surface processes, influences of atmospheric and oceanic changes on land ice, and boundary conditions such as subglacial topography. New computer models can also more accurately simulate glacier and ice sheet evolution. Summary Rapid Arctic ice loss is underway, and future ice loss and sea level rise are guaranteed. Research continues to better understand and model physical processes and to improve projections of ice loss rates, especially after 2050
A metaheuristic for the containership feeder routing problem with port choice process
Get PDFIn this paper, we focus on understanding the joint problem of container ship route generation and consolidation center selection, two important sub-problems influencing the effectiveness of the liners shipping industry, which addresses the ship-routing problem. Two different metaheuristics procedures are presented that both consist of two stages: a solution construction phase (either nearest neighborhood with greedy randomize and Clark and Wright with greedy randomize selection) and a solution improvement phase, based on local search. Both metaheuristics are compared in terms of quality of solution, robustness analysis and computing time under variety of instances, ranging from small to large. A thorough comparison evaluation uncovers that both metaheuristics are close-to-each other. An argument in favor of the nearest neighborhood with greedy randomize approach is that it produces better performance than Clark and Wright configuration. Additionally, through sensitivity analysis, we investigate and test two hypotheses in this paper
