260 research outputs found

    Analysis of Somaclonal Variation in Cinchona succirubra Using Random Amplified Polymorphic DNA (RAPD)

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    Regenerants of C. succirubra were examined by RAPD analysis to determine theoccurrence and extent of somaclonal variation. Plantlets were regenerated by nodal culture. DNAwas extracted from the parent plant and regenerants of C. succirubra. Fifteen base syntheticoligonucleotides from Operon were chosen that gave multiple polymerase chain reaction (PCR)amplification products using cinchona DNA from parent plants grown in the field and plantlets after 4and 5 passages or 4 and 5 month old in vitro culture. The results showed that all of morphologicalcharacters on regenerated plants were clonally uniform and all of the RAPD profiles were generallymonomorphic, stable, and similar to parent plants. These results demonstrated that RAPD can beused successfully to determine the somaclonal variation among regenerated plants

    Studi Pendahuluan Kualitas Air Untuk Pengembangan Budidaya Perikanan di Kecamatan Sampoinit Aceh Jaya Pasca Tsunami

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    The preliminary study of water quality assessment for developing aquaculture effortwas done in Sampoinit sub district for one week (1-8 December 2007). The objective of the presentstudy is to evaluate the suitability of sites for developing of aquaculture. The explorative surveymethod was used in this study by determine of several sampling points at identified sites. Thesurvey was covered four villages i.e. Meunasah Kulam, Crak Mong, Krueng No dan Pulo Raya. Theresults show that Meunasah Kulam, Crak Mong, Krueng No dan Pulo Raya were suitable forbrackish water aquaculture of Scylla serrata, Mugil sp, Tilapia mossambica), Tilapia nilotica andChannos channos, while Crak Mong was suitable for freshwater aquaculture of Clarias batrachusand Channa striata. A semi intensive of aquaculture was suitable to be developed

    Aktivitas Biostatik Torosflavon D Dari Cassia Torosa Terhadap Beberapa Cendawan Patogen Tanaman

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    This research includes isolation of flavonoid Torosflavon D from Cassia Torosa, mediaformulation for pathogenic fungi growth, regeneration of pathogenic fungi, and bioactivity test of theisolated flavonoid on some pathogenic fungi. The flavonid bioactivities tests indicate that theTorosflavon D (5ppm) only effective to inhibit the radial growth of Fusarium oxysporum fsp.Lycopersici (48.75%). Statistical test (anova) indicates that concentration of the flavonid significantlyinhibit the fungi radial growth. Duncan test proves that 5 ppm concentration of Torosflavon D 5inhibits the growth of Fusarium oxysporum fsp. Lycopersici colonies optimally

    Makrozoobentos Sebagai Indikator Biologi Dari Kualitas Air Di Sungai Kumpeh Dan Danau Arang-Arang Kabupaten Muaro Jambi, Jambi

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    This research is used for know the water quality in Kumpeh River and Arang-arang Lakein Kumpeh district based on macrozoobenthic as the biological indicator. To know the communitystructures of macrozoobenthic so it used the analysis of Diversity Index, which uses the Shannon –Weaner Diversity Index. Based on the result of these identification, it was found the quality ofmacrozoobenthic index diversity for Kumpeh River and Arang-arang Lake were between 1.0 - 1.5 andboth of them has been classified as the moderately polluted group. The index of macroozobenthicdiversity of Kumpeh River are about 1.21 and Arang-arang Lake about 1.19 and 1.33. The existencesof Indicator species like Chironomous sp., Scatella sp. And Branchiura sowerbyi are also indicate thatboth of water was polluted

    Pola Aktivitas Harian Lutung (Presbytis cristata, Raffles 1821) di Hutan Sekitar Kampus Pinang Masak, Universitas Jambi

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    jelajah harian meliputi day range (DR), maximum radius (MR) dan night position shift (NPS) di hutan sekitarKampus Pinang Masak, Universitas Jambi. Pengamatan lutung dilakukan dengan metode scanning mulailutung aktif pada pagi hari sampai sore hari. pengamatan dilakukan selama sepuluh hari berturut-turut. Hasilpenelitian menunjukkan rata-rata persentase aktivitas lutung dalam satu hari untuk makan 57,7%, pindahtempat 10,2%, istirahat 19,4% dan aktivitas lainnya 12,7%. Jauh pergerakan harian lutung di hutan sekitarkampus pinang masak, universitas jambi yaitu rata-rata day range (DR) 669,8 m, night position shift (NPS)241,9 m dan maximum radius (MR) 336,7 m. Jenis pohon yang sering digunakan lutung sebagai tempat tiduradalah karet (Hevea brasiliensis), sengon (Albizzia stipulata) dan spesies x (tidak teridentifikasi). Sedangkanjenis tumbuhan sumber pakannya adalah karet (Hevea brasiliensis), sengon (Albizzia sputulata) dan petai(Parkia speciosa)

    PENGARUH KONSENTRASI STARTER Acetobacter xylinum TERHADAP KETEBALAN DAN RENDEMEN SELULOSA Nata de Soya

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    Soybean wasted water is the disposed water from the process of making soybean. During these years thewater produced by soybean production is disposed to the environments, it will cause stink smell and pollutethe environments. In order to solve this problem,the wasted water can be utilized to make Nata de Soya. Natade Soya is one of the food products resulting from bactria Acetobacter xylinum. Making Nata de Soya, themost important thing should be considered carefully the starter concentration of Acetobacter xylinum given towasted water. This research is purposed to find out the effect of concentration of Acetobacter xylinum on thefermentation of soybean wasted water in making Nata de Soya. The process of fermentation is carried out onplastic plate,29 cm lenght and 24 cm width sealed with pieces of newspaper. Fermentation is done on roomtemferature and for fourteen days. The result of the research shows that starter concentration of Acetobacterxylinum influences the cellulose rendement and the thickness of Nata de soya on 15% of starter concentrationof Acetobacter xylinum that produces average thickness 1,32 cm and average sellulosa rendement 22,2 gr/L.Key word : starter concentration of Acetobacter xylinum, Sellulosa rendement,Nata de Soya

    Jumlah Jenis dan Jumlah Individu Semut di Tanah Gambut Alami dan Tanah Gambut PerkebunanSawitdi Sungai Pagar, Riau Ant Diversity and Abundanceon Peat Swamp Forestand Peat Palm Oil Plantation in Sungai Pagar, Riau

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    ABSTRACT.    Peat  lands  conversion  to  palm  oil  plantation  is  a  common  phenomenon  in  Riau  Province. The  quality  and  fertility  of  peat  depends  on  the  level  and  depth  of  peat  which  was  related  to decomposition process. The objective of the research  was to findout the effect of peat  land conversion on antabundance anddiversity. The study was conducted on July 2012on peat swampforest and peat palm oil plantation. Ants were collected by pit fall traps method. The result showed that the number ofant specieson peat swampforest found in this study  was 53 species from 316 individuals, while in the one year old palm oil plantation found 24 species from 237 individuals. Ant species diversity and abundance in  peat swampforest  was muchhigher  thanthat  on  one  year  old  palm  oil  plantation  on  peat  soil.  The composition  and  abundance  of  ants  seem  relatedto physical  and  chemical  properties  of  thepeat  land, especially temperature and pH. Keywords : Ants, Peatlands, Palm oil ABSTRAK. Konversi tanah gambut menjadi kebun sawit merupakan fenomena umum di Provinsi Riau. Kualitas  dan  kesuburan  tanah  gambut  tergantung  pada  tingkat  pelapukan  dan  kedalaman  gambut  yang berkaitan dengan proses dekomposisi. Tujuan dari penelitian ini adalah untuk melihat pengaruh konversi tanah gambut terhadap jumlah jenis dan jumlah individu semut. Penelitian dilakukan pada bulan Juli 2011 pada  tanah  gambut  alami  dan  tanah  gambut  yang  sudah  ditanami  dengan  sawit  selama  satu  tahun. Semut  dikoleksi  dengan  metoda  perangkap  jebak  (pit  fall  trap).  Hasil  yang  didapatkan  menunjukkan bahwa jumlah  jenis  dan  jumlah  individu semut lebih  banyak  didapatkan  pada  tanah  gambut  alami  yaitu sebanyak 53 spesies dan 316 individu semut. Sedangkan pada tanah gambut yang sudah ditanami sawit umur satu tahun jumlah spesiesnya dan jumlah individunya lebih rendah yaitu sebanyak 24 spesies dan 237 individu. Jumlah jenis dan jumlah individu semut tergantung pada faktor fisika kimia tanah terutama temperatur dan pH. Kata kunci :semut,gambut, sawit Â

    Transformasi Genetik Nicotiana benthamiana dengan Gen CP untuk Mendapatkan Ketahanan Tanaman terhadap Peanut Stripe Virus

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    Transformasi genetik tanaman menggunakan Agrobacterium tumefaciens yang membawagen CP (coat protein) telah dilakukan untuk memperoleh tanamn transgenik tahan Peanut Stripe Virus(PStV). Penelitian ini bertujuan untuk mengevaluasi (1) transformasi genetik (proses rekayasagenetika) tanaman dan (2) ketahanan tanaman transgenik model (Nicotiana benthamiana) generasi T0yang membawa gen CP dan gen GUS/NPTII terhadap infeksi PStV. Hasil penelitian menunjukkanbahwa masa kritis untuk regenerasi tanaman transgenik hasil kultur jaringan adalah tahapanaklimatisasi. Tanaman transgenik N. benthamiana yang pertumbuhannya baik dengan batang lebihbesar, akar dan daun yang banyak dalam kultur in vitro, biasanya akan lebih berhasil diaklimatisasi.N. benthamiana transgenik yang membawa berbagai tipe gen CP-1, CP-2, CP-3, CP-4, dan genNPTII/GUS telah dapat dihasilkan. Sedangkan ketahanan tanaman transgenik terhadap PStV terjadiakibat integrasi gen CP dan bukan karena integrasi gen marker atau karena proses-proses lain yangdilalui dalam kegiatan transformasi tanaman dengan bantuan Agrobacterium

    Daily Activity And Microhabitat Preference of Sambar Deer (Cervus Unicolor Kerr, 1792) In The University of Lampung Sanctuary

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    The study to learn the daily activity and microhabitat preference of sambar deer was conducted in theUniversity of Lampung Sanctuary by scanning method. The general condition of the sanctuary was observed by rapidassessment method, vegetation registration was noted by one meter-quadrant. The highest daily activity proportionwere feeding (45.8%), followed by sleeping (30.6%) and resting (23.7%) respectively. Sambar microhabitatpreferences included Imperata- bushed areas for sleeping and resting activities and areas with grasses, shrubs andtrees for feeding activity

    Studi Keanekaragaman Drosophila Sp. di Kota Jambi

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    Telah dilakukan penelitian yang bertujuan untuk mengidentifikasi keanekaragaman spesies Drosophila hasil tangkapan di Kota Jambi. Penangkapan Drosophila dilakukan dengan sistem perangkap menggunakan umpanmedium pemeliharaan TGPR (Tepung-Gula-Pisang-Ragi). Penelitian bertujuan untuk mengetahui keanekaragamanspesies Drosophila yang terdapat di Kota Jambi. Sampel diambil dengan cara purposive sampling dan data dianalisissecara deskriptif. Determinasi dan identifikasi dilakukan dilaboratorium FKIP, UPMIPA dan Laboratrium BioteknologiUniversitas Jambi pada bulan September - Desember 2007. Dari 8 Kecamatan di Kota Jambi, keanekaragamanspesies Drosophila yang diperoleh adalah termasuk Sub Genus Sophophora, jenis Drosophila ananassae, Drosophilabipectinata, Drosophila pallidosa, dan Drosophila melanogaster

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