Jurnal Natural
Not a member yet
304 research outputs found
Sort by
Application of PLS SEM in Evaluations of Mathematics Statistics II Course Online Learning
No full textThe impact of the COVID-19 pandemic on the education sector includes the learning system that cannot be conducted directly (offline). With the change in the learning system, it is necessary to evaluate whether the implemented online learning system can run well and provide results as expected. The level of student satisfaction is one of the factors that can be used to evaluate online learning. This study analyzed the student satisfaction level with online learning for the Mathematics Statistics II course at the Mathematics Department of Andalas University, related to service quality by lecture and the level of student understanding. Data analysis was conducted using causal modeling, namely Partial Least Square Structural Equations Modeling (PLS-SEM). By using The PLS-SEM, it is known that there is a significant and positive effect of service quality on student satisfaction in the Mathematics Statistics II course. In addition, the service quality also affects the understanding level significantly and positively. However, the level of student understanding has no significant effect on the level of student satisfaction. The coefficient of determination (R2) from the structural model of student satisfaction toward the Mathematics Statistics II course online learning is 0.774. This value indicates that the resulting model is good because it has relatively high accuracy
Mammals abundance in Lingga Isaq Hunting Park Landscape, Aceh
Get PDFHunting-park is a conservation area with the unique attraction of periodically hunting in terms of wildlife management. Lingga Isaq Hunting-Park (LIHP) is one of 13 hunting parks in Indonesia. However, the utilization of LIHP as a hunting park is still facing obstacles; two of which are conflicts of interest among authorities and no data of the animals, especially mammals the most hunted creatures in the park. Therefore, a study was conducted to identify the mammalian abundance and distribution. This data would be usefull as essential decision-making for hunting-tourist attractions in LIHP.This study used the transect method, with 12 transect strips evenly distributed in those four function areas; three per each.We found 11 species of mammalian that belonging to 8 families. The protected forest had the highest biodiversity value (H=1.50), while other use areas had the lowest biodiversity value (H=1.16). Macaca fascicularis had the highest abundance (23 individu), while Sus scrofa was the most evenly distributed mammal in the whole landscape. Protected forests and other utilization areas have the highest habitat similarity, identified as having intense anthropogenic activities. The protected forest also has habitat similarity with hunting-park as both areas are legally protected. Nevertheless, there are only Cervus unicolor, Muntiacus muntjak, and Sus scrofa that are highly recommended for hunting tourism attractions
Natural flavoring formulations of straw mushrooms and quality test with variations in temperature and drying time
Get PDFMushrooms contain a substance known as glutamic acid, that produce a savory taste which has the potential to be developed into a natural flavoring. The research began with the determination of amino acid levels in three phases of harvesting straw mushrooms, namely: the egg phase, the bud phase and the adult phase, by using High Performance Liquid Chromatography which aims to determine the harvesting phase with the highest glutamic acid content. The test results of amino acid levels showed the adult phase contained the highest glutamic acid levels of 49.08 mg/g. Furthermore, the manufacture of straw mushroom flour was performed with temperature variations of 60 C and 70 C and drying times of 6, 7 and 8 hours. Then the flavoring formulation was completed by mixing mushroom flour and other dry additives namely shallots, garlic, white pepper, tapioca flour, salt and sugar. The resulting flavoring test is conducted using a description test by assessing the sensory properties of natural flavorings, namely color, aroma, taste and texture. Based on a panelist rating, F2 (60C, 7 hours) has the best score for color (3.88) and aroma (3.32). F5 (70C, 7 hours) has the best score for taste (3.24) and F1 (60C, 6 hours) has the best score for texture (2.08). The conclusion of this study is that mushrooms have a very high potential to be deformalized into natural flavorings, as well as the natural flavoring quality parameters of mushrooms is influenced significantly by temperature parameters and drying tim
Immobilization of urease from Phaseolus vulgaris L. seeds using calcium alginate as a support matrix
Get PDFUrease is an enzyme that functions as a catalyst in the hydrolysis of urea into ammonia and carbon dioxide. The industrial sector has made extensive use of urease. To date, enzymes are used in free form, deemed less effective. Therefore, enzymes are used in immobilized form because they can be utilized repeatedly. This research aimed to isolate urease from kidney bean (Phaseolus vulgaris L.) seed and immobilize it using a Ca-alginate support matrix and a trapping technique. Eight days were devoted to germinating kidney bean seeds to begin the investigation. Isolation of crude urease extract from kidney beans was carried out using phosphate buffer pH 7. It was then immobilized with Ca-alginate at different concentrations of Na-alginate and contact times The crude free and immobilized urease extract was further characterized including pH, temperature and stability of repeated use. The urease activity was determined using the Nessler method using a spectrophotometer. The results demonstrated that urease immobilization from kidney bean seeds with a Ca-alginate matrix was most effective at a concentration of 5% Na-alginate and a contact period of 60 minutes, yielding a value of 5.92 U/mL. The optimal pH of free and immobilized urease was 7 and 8, respectively, and temperatures of 35 and 40 C, respectively. The immobilization of urease from kidney bean seeds using a Ca-alginate support matrix increased the stability of recurrent use by fivefold, while the relative urease activity remained at 52%
Comparison of two DNA extraction methods for dry Leaf Dipterocarpaceae
Get PDFDNA extraction is the first step in obtaining high-concentration and high-purity DNA that can use in the subsequent steps. Because the leaf structure is challenging and contains several secondary metabolites that can affect DNA extraction results, DNA extraction on dry leaves of Dipterocarpaceae is considered problematic. This research aims to find a suitable method for extracting DNA from the preserved Dipterocarpaceae leaves. Preserved leaves of Dipterocarpaceae in silica gel will dry up, making them tough to destroy. Perform DNA extraction is generally using CTAB buffers or extraction kits. According to the results of this study, extraction with modified CTAB buffer result in DNA with higher concentration and purity values than DNA extracted with the kit. DNA isolated from the modified CTAB can be used as a template in the PCR process to amplify the matK gene
Anticancer activity and apoptotic induction of Chromolaena odorata Linn leaves extract and fractions on hepatocellular carcinoma cell lines (HepG2)
Get PDFThe purpose of this study is to screen and to evaluate anticancer activity and apoptosis induction of Chromolaena odorata (C. odorata) leaves extract and its fractions on HepG2 cancer cell lines. The C. odorata leaves were extracted by maceration using ethanol 80% then fractionated by using n-hexane, ethyl acetate and ethanol. MTT assay method was used to evaluate anticancer activity and flowcytometri method used to evaluate the induction of apoptosis. The phytochemical analysis and characterization of the major compound of the extract and the fractions were done by using Liquid Chromatography-Mass Spectroscopy (LCMS). The phytochemical analyis showed that the crude ethanolic extract and fractions of C. odorata leaves contains alkaloid, flavonoids, phenolic, steroids, tannin and saponin. The result of LCMS shown the major compounds of the extract and fractions are 5,7,8,3,4-Pentamethoxyflavonone, 1-Carboethoxy--carboline, 3-Methylcanthin-2, 6- dion, Canthin-6-one. In the n-hexane fraction obtained 5,7,8,3,4-Pentamethoxyflavonone, Methyl ophiopogonanone A and Pd-C-. Meanwhile, in the ethyl acetate fraction contained, 3-(3,4-Dihydroxybenzyl)-7-hydroxychroman-4-one, 3-(4'-Hydroxy-benzyl)-5,7-dihydroxy-6-methyl-8- methoxy-chroman-4-one, Methyl opiopogonanone A and Sinensetin. The ethanol fraction, contained the compound 3-(3,4-Dihydroxybenzyl)-7-hydroxychroman-4-one, 3-(4'-Hydroxy-benzyl)-5,7-dihydroxy-6-methyl-8- methoxy-chroman-4-one and Methyl opiopogonanone A. The crude ethanolic extract, n-hexane fraction, ethyl acetate and ethanol fraction extract have anticancer activities with IC50 value of 23.44 mg/mL, 84.52mg/mL; 88.51mg/mL; and 167.49 mg/mL respectively. This research suggests the crude ethanolic extract of Chromolaena odorata leaves has a potential candidate for hepatocarcinoma treatment
Anatomical changes of Ipomoea reptans due to mercury uptake and accumulation in contaminant soil
Get PDFHeavy metal contaminants like mercury is a serious problem to human, animals, and some plants health. Phytoremediation is an alternative technique, which can remediate the contaminants from soil using a hyperaccumulator plant. The goal of this research was to study anatomical changes of plant main organs (roots, stems, and leaves) of Ipomoea reptans which are assumed as a hyperaccumulator plant that grows in mercury contaminant. The mercury concentration of the growth medium was 0, 61.871, 92.258, and 107.046 ppb. Ipomoea reptans were harvested after 27 days. The anatomical changes of the plants main organs were observed by preparing the cross-section of roots, stems, and leaves of I. reptans. The result showed that mercury treatment has caused anatomical damage at the xylem vessel of the root, and decreased bulliform cell size. The anatomical damage was found only in the root of I. reptans. The mercury concentration in media decrease to 0 ppb (P0), 50,420 ppb (P1), 58,583 (P2), and 96,120 (P3)
Seed size selection from M3 mutant soybean offspring of Kipas Merah Bireun variety in Aceh province
Get PDFKipas Merah Bireun is one of the superior soybeans which was once widely cultivated in Bireun Regency, Aceh Province, used to be as a centre of origin of Kipas Merah Bireun variety. The weaknesses of the Kipas Merah Bireun variety are relatively small seed size (dry weight 12 g/100 seeds), long harvest period and less resistance to drought stress. Therefore it is necessary to improve the quality of Kipas Merah Bireun variety soybeans in order to get soybeans with better properties, one way is to use mutation techniques. The objectives of the study reported here were to evaluate the genetic variability for seed size of single plant selection for large seed in the mutants of gamma irradiated population. The research successfully selected 41 lines soybean mutant with both of high weight seed per plant and large seed size. Several mutant lines of Kipas Merah Bireun were promising to be investigated and developed further in accordance with attempts to increase productivity in Aceh province. Therefore, further research is needed to see how the growth and production of selected mutant soybeans with large seeds in this study in various locations and planting seasons
Optimizing the gonadal performance of broodstock Helostoma temminckii with addition of enrichment ingredients in feed
No full textThe problem in the development of Kissing gourami fish culture was quality seeds that cannot be available continuously. Purpose of this research was to examine the need for additional enrichment materials in feed that can optimize the process of gonad maturation of Kissing gourami fish. Treatment A=Commercial feed added with Vegetable Oil, Glutathione, and Egg White; B=Commercial feed added with Vegetable Oil, Vitamin E 300 mg/Kg, Glutathione, and Egg White; C=Commercial feed added with Vegetable Oil, Vitamin E 500 mg/Kg, Glutathione, and Egg White. and D=Commercial feed added with Vegetable Oil, Vitamin E 700 mg/Kg, Glutathione, and Egg White. The results showed that all treatments with the addition of glutathione and vitamin E fortifications with levels of 300 mg-700 mg gave the effect of increasing IGS, IHS, higher fecundity and larger egg diameter than treatment A. The amount of initial IGS increase in treatment A was 33.49%, treatment B was 172.52%, treatment C was 208.60% and treatment D was 691.20%. While the IHS value at the end of the study decreased in treatment A by 35.30% and treatment C which also experienced a decrease in IHS by 26.23%. While in treatments B and D there was a not too large increase, in the range of 42.56% (B) and 5.66% (D)