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Utilization of shallot extract and administration of indole acetic acid to Chrysanthemum sp in vitro culture
No full textChrysanthemum sp is a plant that has the potential to be developed in Indonesia, apart from being an ornamental flower, it can also be used as a herbal medicine. One of the efforts to develop chrysanthemum can be done in vitro by using organic growth regulators such as shallot extract and indole acetic acid (IAA). The research aims to identify the effect of shallot extract and IAA on the growth of Chrysanthemum sp. The research was conducted at UPT Central Horticulture Seed Center, Medan Johor, in December 2019 - January 2020. The research used a factorial Completely Randomized Design (CRD) with 2 factors, namely the first factor was shallot extract which consisted of 4 levels, namely 0, 35, 70, and 105 g/L water. The second factor was IAA which consisted of 4 levels, namely 0.6, 0.9, 1.2, and 1.5 mg/L water. The results showed that the administration of shallot extract had no significant effect on the growth of chrysanthemum, but the administration of IAA had a significant effect on the number of leaves and plant height with the best concentration at 0.6 mg/L water. The interaction of the two treatments also had no significant effect on the growth of chrysanthemum
Waterlogging and restricted-below ground aeration on photosynthetic performance and root elongation rate of RRIM 600 and RRIT 251 rubber genotypes (Hevea brasiliensis Mull. Arg.)
No full textDue to extended inundation, waterlogging and restricted below-ground aeration cause inhibition in plant growth performance. This study examined two different rubber clones, RRIM 600 and RRIT 251, for waterlogging and restricted below ground aeration. To evaluate the plant performance under the stress conditions, net photosynthetic rate (PN), maximum quantum yield photosystem II (Fv/Fm) and root elongation rate were observed. RRIM 600 has a higher photosynthetic performance under normal conditions. However, the PN rate and Fv/Fm ratio trend showed that RRIM 600 seems to have difficulties recovering after exposure to restricted-below ground aeration. Although RRIT 251 had a lower tendency of PN rate under normal conditions, the PN rate and Fv/Fm ratio of this clone showed fast recovery. RRIT 251 also performed a higher trend of root elongation rate under both stress conditions than RRIM 600
Implementation of Winsorizing and random oversampling on data containing outliers and unbalanced data with the random forest classification method
Get PDFMany researchers conduct research using the classification method, to find out the best method for predicting the class of an observation. Some of these studies explain that random forest is the best method. However, the classification of data containing outliers and unbalanced data is a complicated problem. Many researchers are also conducting research to deal with these problems. In this study, we propose a winsorizing to deal with outliers by replacing the outlier values with the upper and lower limit values obtained from the interquartile range method and random oversampling to balance the data. It is also known that cases of the Human Development Index (HDI) in regencies/cities in eastern Indonesia vary widely, so cases of HDI in these areas can be used as case studies of data containing outliers and unbalanced data. The purpose of this study was to compare the performance of the random forest before and after the data were applied to the winsorizing and random oversampling to predict HDI in districts/cities in eastern Indonesia. Classification method random forest after handling data containing outliers and unbalanced data has better performance in terms of accuracy and kappa values, which are 96.43% and 93.41%, respectively. The variables of expenditure per capita and the mean years of schooling are the most important
The effect of plant growth promoting Rhizobacteria treatment on germination and seedlings growth of chilli
No full textThe aim of the research was to study the effects of biological seed treatment utilization rhizobacteria on seed germination and seedling growth, furthermore to figure out the competence of rhizobacteria isolates to dissolve phosphate, produce acetic indol acid (IAA) and siderophore. 18 rhizobacteria isolates used in this research, each isolates was anlayze in vitro; IAA, phosphate and siderophore. Then carried out the in vitro test, the pepper seeds were inoculated with rhizobacteria isolates suspension then germinated. Seedlings were transpalated into a plastic pot filled with medium soil and compost (2:1). The germination was observed everyday until 14 days, the seedlings was observed at 4 and 6 weeks after transplating. Based on the study, all the rhizobacteria isolates produced IAA, 13 rhizobacteria isolates capable to dissolve phosphate and 12 rhizobacteria isolates produced siderophore. Seed treatment using RBNA 14, RBNA 13, RBKB 5, and RBSPA 14 adequate to increase the germination in seed viability and vigor also increase seedling growt
Intercropping system of Capsicum annum L. and Tagetes erecta with Mycorrhizal application and cow waste compost
No full textFactors that cause fluctuations in chili production are nutrient content and attack by plant disturbing organisms (PDO). To overcome pest attacks can be done by planting refugia which serves as an alternative that will bring in natural enemies. In addition, the application of cow dung and mycorrhizal compost as biological agents will be able to maintain soil quality and plant growth. This study aims to determine the effect of cow waste compost and arbuscular mycorrhizal fungi (AMF) on the growth and production of red chili plants intercropped with refugia plants. The method was a factorial randomized block design consisting of 2 factors, namely: cow waste compost and AMF. Cow waste compost consists of 5 levels, namely: K0 = without giving cow waste compost, K1 = cow waste compost at a dose of 5 tons/ha-1, K2 = cow waste compost at a dose of 10 tons/ha-1, K3 = cow waste compost at a dose 15 tons/ha-1, K4= cow waste compost dose of 20 tons/ha-1. Meanwhile, arbuscular mycorrhizal fungi consisted of 4 levels, namely: M0= without AMF, M1= AMF 50 kg/ha-1, M2= AMF 100 kg/ha-1, M3= AMF 150 kg/ha-1. The results showed that AMF had a most significant effect on the number of fruits per plant, production per plant, and production per plot, but not significantly different on plant height, some branches, and flowering age of Laris variety red chili. AMF treatment with 150 kg/ha (M3) dose had the highest average value for all observed parameters
Acute toxicity evaluation of ethanolic extract of the leaves of Anredera cordifolia in wistar rats (Rattus Norvegicus)
Get PDFAnredera cordifolia (Binahong) is one of the medicinal plants that has been widely used for its properties of inhibiting the growth of microorganisms, decreasing blood uric acid, and healing wounds. This study was conducted to evaluate the acute toxicity effects of ethanolic extract of binahong to support its use as a medicinal plant. Ethanolic extracts of binahong leaves were prepared. Twenty-five white male rats (Rattus norvegicus) were divided into 5 groups and administered with a single dose of Na-CMC suspension or a dose of 300, 600, 1200, or 2400 mg/kg BW of binahong ethanolic leaf extract, respectively. Toxicity symptoms were monitored at three-time intervals: -1, 1-2, and 3-6 hours after the administration. A macroscopic evaluation of the rats liver was carried out to identify any lesion. The rats liver and whole-body weights were determined to assess the weight reduction. The results showed that there was no dead rat in any of the groups, indicating that no LD50 was identified. Also, there were several toxic effects observed including a decrease in movements, somatic response, insensitivity, and stool consistency. There was no evidence of a lesion on liver macroscopy. However, the weights of rats livers increased in tandem with the reduction in their body weights. The findings from this study reveal that single dose of binahong ethanolic extract ranging from 300 to 2400 mg/kg BW are safe, while minimal toxic effects were observed
Coconut oil effect on the hygroscopic properties of Simalambuo wood (Lophopetalum spp.)
Get PDFSimalambuo (Lophopetalum spp) trees are endemic to Southeast Asia and widely grow in the natural forests in Nias island. Local people whose experience of utilizing the simalambuo wood have claimed that the wood easily absorbs water. Throughout our searches, not many efforts to modify the physical properties of the simalambuo woods have been carried out yet. Therefore, in this study, we modified simalambuo wood by thermal treatment in coconut oil-ambient conditions. The heat treatment became an option to support the efforts in reducing the use of non-environmental-friendly materials and methods. The simalambuo wood was treated under coconut oil at 130-210oC for 6 hours. Coconut oil uptake was up to 174%, while the leaching test (AWPA E-06 standard) showed the uneasily permeate out from the wood and high retention differently in terms of treatment and cooling temperature. Heat treatment with coconut oil affected water absorption ability and improved dimensional stability of simalambuo wood. Water absorption reduced from 162% to 16%, and dimensional stability increased from 8.4% to 4.2% at 192 hours after being soaked in water. The higher the difference between treatment and cooling temperature, the better-affected water absorption ability was and the higher dimensional ability could be achieved
Differential tolerance of Trichoderma harzianum and Rhizoctonia solani towards silver nanoparticles: potential for agricultural applications?
Get PDFIn the previous study, we examined the effect of silver nanoparticles (AgNPs) on beneficial soil fungus including Trichoderma harzianum (T22), and pathogenic soil-borne fungus, Rhizoctonia solani (AG3-PT). The result exhibited that T. harzianum (T22) is tolerance towards AgNPs. On the other hand, the pathogenic fungi, R.solani (AG3-PT), is more sensitive to AgNPs. T. harzianum is well known as biocontrol agent to suppress R. solani. Therefore, in this study we investigated the combination of T. harzianum (T22) and AgNPs at low concentration to control two strains of R. solani (AG3-PT and AG2-1). The effect of AgNPs at two different levels (20 mg L-1 and 50 mg L-1) was examined over the growth of the two strains of R. solani and T. harzianum (T22) using dual culture technique. The results shows that this combination have a potential to reduce colony growth of R. solani (AG2-1) at higher AgNPs concentration. However, it was not the case for R. solani (AG3-PT). It can be concluded that AgNPs toxicity depend on several factors including species strain and the size of AgNPs particle
Evaluation of wound healing potential of a sea cucumber (Actinopyga mauritiana) extract in mice (Mus musculus)
Get PDFSea cucumbers are a marine source with biological activities that have been used in food as well as medicine in many Asian countries. Actinopyga mauritiana is one of the sea cucumber species with limited research about its bioactive activities. In this study, the wound healing activity of A. mauritiana extract in the form of cream, gel, and serum formulations in mice was investigated. The histopathology test was performed to evaluate the fibroblast and collagen dense levels in wound healing. Phytochemical screening has been carried out by the qualitative test of alkaloids, flavonoids, glycosides, steroids, and saponins compounds. The A. mauritiana ethanol extract (SCAE) was added into cream, gel and serum formulations with various concentrations (3, 6, and 9%) and applied to the wounded area of mice. The wound healing activities and histopathology results indicated that serum 9%-SCAE performed the highest decrease of wound length 0.55 0.32 compared to other groups with a density level of fibroblast and collagen are 10.00 1.00 and 10.67 0.00, respectively. To the best of our knowledge, this is the first report on the wound healing activity of A.Mauritiana, which demonstrated promising therapeutic agents for wound healing and cosmetics applications in the future