Jurnal Ilmu dan Teknologi Peternakan Tropis
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Physicochemical Properties and Antioxidant Activity of Multiflora Honey from Kerinci Regency, Jambi
aim of this study was to examine the physicochemical properties and antioxidant activities of multifloral honey (Apis dorsata forest honey) and (Apis cerana cultivated honey) from Kerinci, Jambi. The analysis consist of the intensity of colour, pH, viscosity, water content, ash content, sugar content, HMF content, acidity and antioxidant activity. The results showed that forest honey had light amber colour while cultivated honey had extra light amber colour. The pH, viscosity, water content, sugar content, HMF content, and acidity in forest honey were still in compliance with SNI standard, but the ash content was higher than the SNI standard. Cultivated honey had pH, sugar content, and HMF content in the range of SNI standar. However, the viscosity, water content, ash content, and acidity in cultivated honey were not in compliance with SNI standar. Forest honey and cultivated honey had antioxidant capacities about 16.74 mgVCE/g and 16.60 mgVCE/g, respectively. Meanwhile the antioxidant activity were 63.80% and 63.28%, respectively. Forest honey had more physicochemical aspects that were still in compliance with SNI standards compared to cultivated honey. The antioxidant activities of Kerinci honey, both forest honey and cultivated honey, were higher than the results of previous honey studies in other location.
Keywords: antioxidant activities; cultivated honey; forest honey; multifloral honey; kerinci hone
Chemical and Biological Characteristics of Whole Crop Corn Silage with Rice Bran and White Cabbage Additives during Ensilage
ABSTRACT
The objective of this work was to evaluate whole crop corn silage quality throughout the ensilage periods with rice bran and white cabbage as additives. Whole crop corn from 60 days aged was chopped ±2-5 cm, and added rice bran, white cabbage and without additive (control) then put on plastic bag under anaerobic condition for 1, 2, 3, and 4 weeks. Silages were analyzed in physical characteristics (flavor, color, texture); chemical characteristics (dry matter/DM, DM recovery, organic matter/OM, pH value, fleigh value, water-soluble carbohydrate/WSC), and biological characteristics (type and number of lactic acid bacteria (LAB)) weekly. The results showed that additives affected (
Effectivity of lime leaves (Citrus sp.) in inhibiting Escherichia coli: a meta-analysis
Antibiotics Growth Promoter (AGP) are currently banned. Citrus leaves have active secondary metabolites that have the potential to be used as phytogenic feed additives. Many in vitro studies have reported the activity of lime leaves against E coli. Therefore, this study aims to integrate various studies in order to obtain comprehensive information on the activity of lime leaves against E coli with a meta-analysis. This research was conducted by collecting studies of citrus leaves from various species, origin, extraction methods, and different antibacterial test methods and comparing the treatment of citrus leaves with controls in inhibiting E coli by looking at the diameter of the inhibition zone. The results showed that kaffir lime leaves as an alternative phytogenic feed additive for AGP were effective in inhibiting E. coli. The inhibitory ability of E. coli bacteria was based on differences in the origin of lime leaves and extraction methods, while differences in levels, types of lime leaves, and antibacterial test methods did not affect the inhibition of E. coli. Citrus leaf extract at a low concentration of 5 mg/ml was able to inhibit E coli bacteria.
Keywords: escherichia coli, lime leaf, meta-analysi
Characteristics and Adaptation Mechanisms of Saline-Tolerant Fodder Plants
Coastal areas and small islands in Indonesia, reaching approximately 17,000 islands, will be directly affected by increasing sea levels, seawater intrusion, and salinization. This paper provides insight into salinity and its effect to plant, saline-tolerant animal feed crops' characteristics, adaptation mechanisms, and some proposed strategies to deal with salinity issues. This research was conducted through literature study and data were analyzed descriptively. Salinity affects the photosynthesis process, thereby decreasing the net Photosynthetic Rate (Pn), the stomatal aperture, Fv/Fm (efficient quantum), and quantum yield (light intensity/rate of photosynthesis). Salt-stressed plants can be seen from the severity by measuring the levels of Cl and Na in the roots, leaves, and chlorophyll. Plant tolerance to salinity is divided into two groups: glycophytes that are tolerant of low salt levels and halophytes that are tolerant of high salts. Salinity inhibits cell enlargement and division, increases plant mass, and increases protein and nucleic acid production. The plant adapts to saline stress, including osmotic adjustment, stress protein, free radical oxidative species (ROS) removal, ABA synthesis, and cross-adaptation. Increasing the productivity of animal feed in saline areas could be achieved in various ways by modifying water, land, chemical and biological properties, and farmer habits
Use of Propolis Extract as an Ingredient for Skin Cancer Cell Apoptosis Transfersome Cream with Gelatin as an Emulgator
Transfersome has been commonly used to increase the penetration of drugs derived from natural ingredients. Propolis is a natural ingredient with many benefits, such as antimicrobial, antiviral, anti-inflammatory, antitumor, antioxidant, and anticancer. In various in vitro studies, propolis showed proapoptotic activity in cancer cells. Gelatin is a chemical compound that can be used as an emulsifier because it has a hydrophobic part in the peptide chain. This study aimed to determine the optimal gelatin content based on physical tests as an emulsifier in the manufacture of emulsions for transfersome cream ingredients. The research method used was an experimental laboratory with 6 gelatin concentration treatments, namely 1% (P1), 2% (P2), 3% (P3), 4% (P4), 5% (P5), and 6% (P6) with 3 replicates. To make the emulsion, 5% propolis extract was added. The results of statistical analysis showed that the manufacture of emulsions using different concentrations of gelatin had a very significant effect (
The effect of red ginger extract as a feed additive of broiler quail
This study aims to evaluate the effect of dietary inclusion of red ginger extract (Zingiber officinale var. rubrum) on total coliform of intestinal bacteria, digestibility, and performance of broiler quail. A total of 100 unsexed broiler quail were randomly assigned into five groups of treatment with four replications (5 quail each). Dietary treatments applied were P0 = basal feed + 0 mg/kg red ginger extract ; P1 = basal feed + 500 mg/kg red ginger extract; P2 = basal feed + 750 mg/kg red ginger extract; P3 = basal feed + 1000 mg/kg red ginger extract and P4 = basal feed + 4000 mg/kg tetracycline. Experimental feed were given to quail after 2 to 6 weeks old. Data were analyzed to test of variance and followed by Duncan test. The results reveal that the supplemented of red ginger extract in the feed had a significant effect (P0.05) on growth performance. In conclusion, red ginger extract has a positive effect on total coliform of intestinal bacteria and nutrient digestibility but has no significant effect on growth performance broiler quail
Ukuran Populasi Aktual, Ukuran Populasi Efektif, dan Laju Inbreeding Per Generasi Kambing Kacang di Kabupaten Muna Barat, Sulawesi Tenggara
Sperm Quality of Native Chickens with Different Extenders at a Storage Temperature of 5°C
Semen extenders are developed to increase ejaculate volume, provide essential nutrients, and maintain spermatozoa quality and viability during storage at both refrigeration temperatures and cryopreservation. This study aimed to analyze the spermatozoa quality of native chickens using different extenders at a storage temperature of 5°C. The research was conducted at the Regional Breeding and Animal Feed Center (UPTD Balai Perbibitan dan Pakan Ternak), Department of Food Crops and Animal Husbandry, Southeast Sulawesi Province, from January to February 2024. A completely randomized design (CRD) was used in this study, consisting of four treatments and five replications (replications refer to semen collections). The semen was stored at 5°C and evaluated at different storage durations (0, 6, 12, 18, and 24 hours). The treatments included different semen extenders: (P1) physiological saline solution, (P2) coconut water, (P3) tris-egg yolk, and (P4) a combination of 50 ml coconut water + 50 ml tris-egg yolk. The research variables included macroscopic evaluation of fresh semen (semen volume, color, odor, consistency, and pH) and microscopic evaluation of fresh semen (mass motility, individual motility, intact plasma membrane (IPM), spermatozoa concentration, and spermatozoa abnormalities). Post-dilution evaluations included individual motility and IPM. The results showed type of extender significantly affects the quality of native chicken spermatozoa during storage at 5°C. Semen storage at this temperature reduces motility and intact plasma membrane (IPM) percentage due to cold shock, which disrupts membrane integrity. Tris-egg yolk extender (P3) is the most effective in maintaining motility and membrane integrity, as its lecithin and lipoproteins protect spermatozoa from cold shock, maintain osmotic balance, and supply ATP for viability. Thus, tris-egg yolk (P3) is the best extender for preserving native chicken spermatozoa quality at 5°C.Keywords: semen extender, spermatozoa quality, storage temperature 5°C, native chickens, intact plasma membran
Genetic Diversity of Simpo and Limpo Cattle Based on Five Blood-protein Locus Polymorphism
Simpo and Limpo cattle is a crossbred cattle of Indonesian local cattle and exotic cattle that popular among traditional farmers in Indonesia. The objective of this study was to identify genetic diversity of Simpo and Limpo cattle based on blood-protein polymorphism. A total of 70 blood samples were collected from unrelated 35 heads of Simpo cattle and 35 heads of Limpo cattle from Grobogan dan Kendal population. The blood plasm was used to identify the blood proteins. Blood protein analysis was performed using Sodium Dodecyl Sulfate-Polyacrilamide Gel Electrophoresis (SDS-PAGE). Observed bands were used to perform allele interpretation of five locus, namely Pre-albumin (Pe-Alb), Albumin (Alb), Post-albumin (Po-Alb), Ceruloplasmin (Cp) and Amylase-1 (Am-1). Results showed there were polymorphism in all of the five blood protein locus in both cattle; 4 of 5 and 2 of 5 of the blood-protein locus studied showed deviation from Hardy-Weimberg Equilibrium (HWE) for Simpo and Limpo cattle, respectively and the average heterozygosity value (H) was 0.478 and 0.362 for Simpo and Limpo cattle, respectively. In conclusion, the study indicated that population of Simpo and Limpo cattle has high genetic diversity.
Keywords: allele frequency; genotype frequency; heterozygosit
Effect of In-Ovo Injection of L-Arginine on Age Embryo Died, Embryo Performance, and Chick Quality of Local Chicken
This study aims to determine the Effect of In-Ovo injection of L-arginine on age embryo died, embryo performance, and chick quality of local chicken. A total of 160 eggs were put into a semi-automatic incubator which has a temperature range of 37-38 oC with a relative humidity of 55-65%. The injected L-arginine solution has a concentration of 0.5% (m/v). In-Ovo Feeding treatment was divided into 4 groups, the first treatment was without injection (control), the second treatment was injection of 0.2 ml L-arginine solution 0.5% (m/v)/egg, the third treatment was injection of 0.4 ml L-arginine solution. 0.5% (m/v)/egg, and Fourth Treatment was injection of L-arginine solution 0.6 ml 0.5% (m/v)/egg. The results showed that embryo mortality and hatchability were lower with L-arginine injection treatment than without injection. All treatments showed no effect on extraembryonic fluid absorption, but there was an increase in embryo weight in the injection treatment 0.2, 0.4, 0.6 ml L-Arginine 0.5% /eggs 2,355 g, 2,577 g, 2,705, respectively. In conclusion, In-Ovo injection of L-arginine in local chicken eggs have a good effect (the beneficial effect) on embryo died and embryo performance. Injection 0.4 ml L-Arginine 0.5% /eggs improved Hatchability and NHCW