Jurnal Ilmu dan Teknologi Peternakan Tropis
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Effect of Thawing Technique on the Quality and Status of Acrosome Reactions
The success of Artificial Insemination is affected by the thawing technique used by the inseminator. The difference in thawing technique can affect the quality of the spermatozoa produced. This study aims to determine the effect of temperature and duration of thawing on the frozen semen quality of Bali and Madura cattle. The material used was 80 frozen semen straws for Bali cattle and Madura cattle. The method used is an experiment with four treatments and 10 replications, that is thawing in water at 37℃ for 30 seconds duration, 28℃ for 30 seconds, 28℃ for 45 seconds and 28℃ for 60 seconds. The variables observed in this study were capacitation, intact acrosome, intact plasma membrane and chromatin damage. The data were analyzed using a Randomized Block Design with replication as a block. The results of the analysis of variance showed that thawing technique had significant effect (p0.05) on the acrosome reacted and chromatin damage.Keywords: thawing technique, capacitation, intact acrosome, intact plasma membrane, chromatin damag
Asosiasi Variasi Genotipe PIT1|HinfI pada Tampilan Produksi Susu Sapi Perah Friesian Holstein Indonesia
ABSTRAK Gen Pituitary Specific Transcription Factor 1 (PIT1) adalah gen yang mengatur kelenjar pituitari dan merupakan regulatory factor hormon pertumbuhan serta hormon lain yang terkait dengan produksi susu. Oleh karena itu, penelitian ini bertujuan untuk mengasosiasikan variasi genotipe PIT1 dengan produksi susu sapi Friesian Holstein (FH) di Indonesia. Sebanyak 42 ekor sapi dipilih sebagai sumber DNA. Variasi genotipe ditentukan dengan metode Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) menggunakan enzim restriksi HinfI. Data produksi susu diambil pada laktasi 1 dan 2 yang distandardisasi ke catatan produksi 305 hari. Sebanyak 3 variasi genotipe terdeteksi yaitu AA, AB, dan BB dengan frekuensi masing-masing 0,21; 0,26; dan 0,52. Pada penelitian ini diperoleh frekuensi alel A sebesar 0,35 dan B sebesar 0,65, dan populasi berada pada kondisi setimbang (p0,05) dibanding AA dan AB pada laktasi 1, namun pada laktasi 2 antar genotipe tidak berbeda nyata. Berdasarkan hasil penelitian maka disimpulkan bahwa variasi genotipe gen PIT1 memiliki potensi untuk digunakan sebagai penanda genetik kemampuan produksi susu sapi FH di Indonesia.Kata Kunci: asosiasi, produksi susu, sapi FH Indonesia, variasi genotip gen PIT1.Association of PIT1|HinfI Genotype Variation on Milk Production of Indonesian Friesian Holstein Dairy Cattle ABSTRACT Pituitary Specific Transcription Factor 1 or known as PIT1, is a gene which function to control the pituitary gland and act as regulatory factor for growth hormone and other hormones related to milk production. The current study was aimed to associate the variant of PIT1 genotype on milk production in Indonesian Friesian Holstein. In total, 42 dairy cows were sampled for DNA source. Phenotypic data of milk yielded from first and second lactation were recorded then standardized to 305 days. To scan PIT1 genotype variation, PCR-RFLP was employed using HinfI restriction enzyme. Three types of PIT1 genotype were found, namely AA, AB, and BB; its frequencies were 0.21; 0.26; and 0.52, respectively. The allele frequencies of allele A and B were 0.35 and 0.65, and the population is in equilibrium condition (p<0.05). Significant associations (p<0.05) were found between genotypes in lactation 1. In this study, BB genotype yielded highest milk compared to other genotypes in lactation 1, but no differences among genotypes were observed in lactation 2. In a conclusion, the variation of PIT1 genotype in Indonesian Friesian Holstein shows potential use for genetic marker to improve milk production in this study.Keywords: association, milk yield, Indonesian Friesian Holstein, PIT1 genotype variatio
Penentuan Lokasi Basis Komoditas Kambing Menggunakan Analisis LQ dan DLQ di Kabupaten Lampung Timur
ABSTRAKPenelitian bertujuan untuk mengetahui kecamatan basis komoditas kambing supaya pemerintah dapat mengembangkan secara maksimal sektor pertanian, khususnya komoditas kambing di Kabupaten Lampung Timur. Data pemotongan dan populasi ternak sepanjang tahun 2016-2020 diperoleh dari instansi terkait yang selanjutnya dianalisis menggunakan Location Quotient (LQ) yang digunakan untuk mengetahui kecamatan basis di Kabupaten Lampung Timur, analisis Dynamic Location Quotient (DLQ) digunakan untuk menentukan kecamatan basis berdasarkan pertumbuhan komoditas kambing di Kabupaten Lampung Timur. Kecamatan unggulan komoditas kambing ditentukan berdasarkan nilai LQ dan DLQ >1 yang memberi kontribusi besar terhadap daerah karena memungkinkan ekspor serta potensi pengembangan komoditas berkembang dengan cepat. Hasil analisis kecamatan unggulan komoditas kambing yakni Kecamatan Marga Tiga, Sekampung Udik, Jabung, Labuhan Maringgai, Mataram Baru, Way Jepara, dan Sukadana. Setelah didapat kecamatan unggulan diharapkan dapat membantu fokus pengembangan komoditas kambing agar tepat sasaran pada kecamatan yang berpotensi berkembang dengan baik.Kata Kunci: DLQ, kambing, komoditas basis, LQ.Determining the Location of Goat Commodity Base Using LQ and DLQ Analysis in East Lampung RegencyABSTRACTThis study aims to determine the sub-district of the goat commodity base so that the government can optimally develop the agricultural sector, especially the goat commodity in East Lampung Regency. Slaughter and livestock population data during 2016-2020 were obtained from relevant agencies which were then analyzed using Location Quotient (LQ) which was used to determine the base sub-district in East Lampung Regency, Dynamic Location Quotient (DLQ) analysis was used to determine the base sub-district based on the growth of the goat commodity in East Lampung Regency. The superior sub-districts for goat commodities are determined based on the LQ and DLQ values >1 which make a major contribution to the region because it allows exports and the potential for developing commodities to develop quickly. The results of the analysis of the leading sub-districts for goat commodities are Marga Tiga District, Sekampung Udik, Jabung, Labuhan Maringgai, Mataram Baru, Way Jepara, and Sukadana. After obtaining the superior sub-districts, it is hoped that it can help focus the development of goat commodities so that it is right on target in sub-districts that have the potential to develop well.Keywords: DLQ, goat, base commodity, L
External and Internal Quality of the Quail Eggs Fed Ration with Different Level of Metabolizable Energy and Protein
Quail eggs are a source of animal protein that the public can consume. In addition to the low price, eggs also contain nutrients needed by the body. So it is necessary to pay attention to the quality of the eggs before consuming them. Egg quality that can be assessed is derived from internal and external quality. The quality of the eggs is influenced by various factors, including the content of the ratio given, especially the energy-protein balance of the feed. This study aimed to assess the external and internal quality of quail eggs with different protein and energy levels. The research was carried out for 42 days in the poultry house of the Animal Husbandry Study Program, Faculty of Agriculture, Djuanda University. The feed ingredients formulated in this study as the basic ration were yellow corn, fine bran, soybean meal, fish meal, premix, DCP, CaCO3, CPO, and Cr-O. The design used was a completely randomized design (CRD) with four treatments and four replications with six quails per cage unit. The research data were analyzed by analysis of variance (ANOVA) if the data showed significantly different results (P<0.05), followed by Duncan's test. The variables observed in this study were egg weight, shell weight, percentage of eggshell weight, eggshell thickness, egg index, egg white weight, egg yolk weight, egg white weight percentage, egg yolk weight percentage, Haugh unit (HU). The results showed that the balance of energy and protein differences were not significantly different in all variables. Based on the research results, it can be concluded that the quail ration with the balance of protein 17% and metabolizable energy 2800 kcal/kg has been able to maintain the internal and external quality of eggs in quail and produce standard egg quality.Keywords: protein energi balance; egg white, yolk, haugh uni
Pengaruh Frekuensi Pemberian Pakan Hijauan yang Berbeda terhadap Produktivitas Ternak Kambing Saanen
ABSTRAKPenelitian ini bertujuan untuk mengetahui penampilan produksi kambing saanen dengan perlakuan pakan hijauan dengan cara mengatur frekuensi pemberiannya (frekuensi pemberian pakan perhari). Materi yang digunakan dalam penelitian ini adalah kambing saanen jantan sebanyak 12 ekor umur 6 bulan, penelitian dilakukan dengan 3 perlakuan masing-masing 4 ulangan, dengan bobot badan awal 15-17 kg. Kambing penelitian diberikan pakan BK (bahan kering) sesuai dengan kebutuhannya (3% x bobot badan). Pakan yang digunakan dalam penelitian ini adalah konsentrat dan rumput gajah dengan perbandingan konsentrat: rumput gajah adalah 70% : 30% dari total bahan kering ransum. Perlakuan yang ditetapkan terdiri dari 3 perlakuan (P1, P2 dan P3) dan 4 kali ulangan (kambing) yaitu frekuensi pemberian pakan hijauan: P1 = frekuensi 1 kali perhari (pukul 08.00), P2 = frekuensi 2 kali perhari (pukul 08.00 dan 11.00 WIB) dan P3 = frekuensi 3 kali perhari (pukul 08.00; 11.00 dan 14.00 WIB). Variabel pengamatan yaitu konsumsi pakan, pertambahan berat badan harian dan konversi pakan. Data dianalisis menggunakan analisis variansi (ANOVA) pola searah dan dilanjutkan dengan uji Duncan’s Multiple Range Test (DMRT) bila terdapat perbedaan. Hasil penelitian menunjukkan rerata konsumsi pakan berturut-turut adalah P1=426,36±7,91; P2=433,56±3,43 dan P3= 431,13±6,72 gr/ek/hari berbeda tidak nyata. Pertambahan berat badan harian berturut-turut P1=59,14±4,73; P2=60;42±3,08 dan P3=58,35±2,54 gr/ekor/hari menunjukkan berbeda tidak nyata. Konversi pakan berturut-turut P1=7,34±0,62; P2=7,38±0,45 dan P3=7,46±0,35 menunjukkan tidak berbeda nyata. Kesimpulan pada penelitian yaitu pemberian pakan hijauan pada waktu yang berbeda tidak memberikan pengaruh yang nyata terhadap produktivitas kambing saanen.Kata kunci: pakan hijauan, frekuensi, kambing, produktivitasThe Effect of Different Forage Feeding Frequency on Saanen Goat's Livestock ProductivityABSTRACTThis study aims to determine the appearance of saanen goat production with forage treatment by adjusting the frequency of feeding (frequency of feeding per day). The material used in this study were 12 male saanen goats aged 6 months, the study was conducted with 3 treatments each with 4 replications, with an initial body weight of 15-17 kg. The research goats were given dry matter based feed according to their needs (3% x body weight). The feed used in this study was concentrate and elephant grass with a ratio of concentrate: elephant grass is 70%: 30% of the total dry matter of the ration. The treatment set consisted of 3 treatments (P1, P2 and P3) and 4 replicates (goats), namely the frequency of forage feeding: P1 = Frequency 1 time per day (at 08.00), P2 = Frequency 2 times per day (at 08.00 and 11.00 WIB) ) and P3 = Frequency 3 times per day (at 08.00; 11.00 and 14.00 WIB). Observation variables were feed intake, average daily gain and feed conversion. Data were analyzed using Analysis of Variance (ANOVA) in a unidirectional pattern and continued with Duncan's Multiple Range Test (DMRT) if there were differences. The results showed that the average of feed intake was P1=426.36±7.91; P2=433.56±3.43 and P3= 431.13±6.72 g/head/day were not significantly different. Average daily gain P1=59.14±4.73; P2=60.42±3.08 and P3=58.35±2.54 g/head/day showed no significant difference. Feed conversion P1=7.34±0.62; P2=7.38±0.45 and P3=7.46±0.35 showed no significant difference. The conclusion of this study were that feeding forage at different times does not have a significant effect on the productivity of saanen goats.Keywo rds: forage feed, frequency, goats, productivit
Effect of Pre Slaughter Fasting On Physical Quality of Broiler Meat
Assessment of meat physical quality is the way to choosing meat. Pre slaughter time is one method to restorate livestock (including poultry) prior to deductions so that animals do not wage for too long at the time of slaughter. This study aims to determine the effect of pre slaughter fasting on the quality of meat produced. This study using 24 samples of broiler chickens. The sample is divided into 4 groups where group 1 without treatment; group 2 4 hours of fasting; group 3 8 hours of fasting; and group 4 12 hours of fasting. The parameters include specific gravity, pH, color, tenderness, and water holding capacity. The result of this study found that fasting on broiler chickens up to 12 hours did not give a significant effect on specific gravity, tenderness, and water holding capacity. Pre slaughter fasting until 12 hours only gives effect to pH and color of meat. The fasting time is 8 - 12 hours before slaughter not recommended because it has the biggest pH possible to get pale, soft, exudative (PSE)
Exploitation reduction and conservation of local beef cattle as a result of demand for beef through massive artificial insemination programs in Indonesia
Indonesian Ministry of Agriculture has made a breakthrough in increasing the cattle population to reduce supply inequality through a SIWAB/SIKOMANDAN program. The program focuses on massive artificial insemination and is supported other activities, namely feed aid, animal health, veterinary public health, and community services. The purpose of the study was to determine the conservation of local cattle genetic resources, increasing population, and economic valuation. The research data was collected from Directorate General Livestock and Health (DGLH) for four years. Mathematics models were used to analyze the target and realization the number of frozen semen distribution, acceptor cows, pregnant cows, and calves born. The incremental capital-output ratio (ICOR) model was used to determine the economic valuation. The results show that the program could conserve local cattle, the number of pregnant cows was 8.17 million heads (87.84%), and the number of calves born was 7,040,160 heads or more than 100% of the target for four years as many as 7,040,000 cattle. The average service per-conception (S/C) value was 2.06 or in the normal range. The economic valuation of government investment could generate economic value added marked with an ICOR < 1 or efficient investment
Addition of various types and levels of vegetable oil on the physical quality of Mozzarella cheese
This study aims to prove that adding various types and levels of vegetable oil has good characteristics in terms of physical quality. The research material was fresh bovine milk, starter cultures, citric acid, rennet, sodium citrate (Na₃C₆H₅O₇), and vegetable oil. The method used is a laboratory experiment using a nested, Completely Randomized Design (CRD). Treatment A, namely the type of vegetable oil, and treatment B, namely the level of vegetable oil. Data analysis used analysis of variance (ANOVA), followed by Duncan's Multiple Range Test (DMRT) if the results showed a significantly different or highly significant effect. The results showed that the addition of vegetable oil (treatment A) had no significant effect (P>0.05) on pH, color (L*a*b*), stretchability, reliability, elasticity, hardness, and free oil. The level of vegetable oil nested in the type of vegetable oil (treatment B) had a highly significant effect (
Study on Egg Quality Control Product at PT. X Through The Statistical Quality Control Method Approach
PT. X does not implement statistical quality control. This study aims to determine the factors that cause damaged egg products at PT. X, knowing the level of damage to PT. X based on the control chart and corrective actions needed to reduce defective products that meet quality standards. This study uses a descriptive method with a case study approach for 30 working days. Data collection techniques used include observation through manual visual inspection, interviews with staff of PT. X and documentation. This study uses quality control analysis tools in the form of check sheets, histogram diagrams, pareto diagrams, control charts and fishbone diagrams. Controlling the level of damage to egg products at PT. X was outside the control limits, namely 1.99% pale shells, 1.80% cracked shells and 7.09% gross shells of the total production. This research suggests that PT. X implements quality control using the SQC method and applies protectors and dividers to all products to prevent eggs from cracking during storage and distribution as well as routinely removing dirt. PT. X needs to conduct training for production employees so that they can carry out the production process accompanied by inspections in accordance with the company's standard operating procedures.Keywords: eggs, statistical quality control, quality contro
Utilization of Pineapple Bromelain Enzymes (Ananas comosus) on Organoleptic, Physical and Chemical Quality of Mozzarella Cheese
This study aims to determine the effect of using pineapple bromelain enzymes with different levels on the organoleptic, physical and chemical quality of mozzarella cheese and to obtain the best levels of bromelain enzymes in the manufacture of mozzarella cheese. This research was conducted from July to October 2021 at the Animal Husbandry Laboratory, Faculty of Agriculture, Kadiri Islamic University. The method used is a completely randomized design (CRD). This study used 4 treatments and 5 replications, namely: T0 (100% pasteurized cow's milk + 1% rennet enzyme), T1 (100% pasteurized cow's milk + 1% bromelain enzyme), T2 (100% pasteurized cow's milk + 3% bromelain enzyme) and T3 (100% pasteurized cow's milk + 5% bromelain enzyme). The research variables included the organoleptic, physical and chemical quality of Mozzarella cheese. Data were analyzed using Microsoft Excel program, then statistical analysis was performed using Completely Randomized Design Experiment (CRD) and analysis of variance, followed by Duncan's Multiple Range Test (UJBD) if there were different results. The results showed that the use of the bromelain enzyme had a very significant effect (P<0.01) on color, aroma, texture, pH, melting power and moisture content of Mozzarella cheese and significantly (P<0.05) on taste, curd weight, yield and cheese weight. The conclusion is that the use of bromelain enzymes with a level of 1% is the best level, namely color, aroma, texture, curd weight, yield, cheese weight and moisture content of Mozzarella cheese.Keywords: bromelain enzyme, mozzarella cheese, organoleptic quality, physical and chemica