Buletin Peternakan
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Performance and Haematological Profile of Broiler Chickens Fed Diet Containing Atung (Parinarium glaberrimumHassk.) Seed Powder
Full text linkThis study investigated the effect of feeding atung (Parinarium glaberrimum Hassk.) seed meal on growth performance and haematology profile in broiler chickens. A total of 140 one-day-old male New Lohmann broiler chicks were randomly assigned to five treatment groups with four replicates and seven birds in each replicate pen. The dietary treatments consisted of feeding the same corn-soybean meal as the basal diet with atung seed powder inclusions at levels of 0, 0.5, 1.0, 2.0, and 4.0%, respectively. The data were statistically analyzed using One-way with five treatments and four replications and continued with Duncan's New Multiple Range Test for data with a significant difference. Results showed that feed intake increased (p<0.05) in broiler chickens fed diets with 0.5 and 1% atung seed powder. At the same time, the inclusion of atung seed powder had no effect on final body weight, weight gain, and FCR. Inclusion of 1 and 2% atung seed powder significantly increased leucocyte counts, as well as the PCV value trend increased with the inclusion of atung seed powder. It can be concluded that atung seed powder additives can be supplemented in broiler chickens feed at doses 1% by improving feed intake and some haematological profiles
Expression of Myostatin Gene in Belgian Blue and Ongole Grade Crossbred Cattle
Full text linkInvestigating Myostatin (MSTN) as a potent inhibitor of skeletal muscle growth and development to produce excessive muscles is extremely essential for livestock breeding. This study aimed to analyze the expression of the MSTN gene and its relationships with genotype and phenotype (normal-muscled vs double-muscled) of Belgian Blue (BB) x Ongole Grade (PO) crossbred cattle. For that purpose, 12 animals from BB, PO, BB x PO F1, and BB x PO F2 cattle (3 animals each) raised at Balai Embrio Ternak (BET) Cipelang Bogor, West Java were used for blood sample collection. Genotyping analysis was performed using the PCR-RFLP method withprimer F: 5’-CTC TTC TTT CCT TTC CAT ACA GAC-3’ and R: 5’-AGG GGA AGA CCT TCC ATG TT-3’, while the MSTN gene expression was analyzed using the qPCR technique. As results, three genotypes: del.11/del.11, +/del.11, and +/+ were detected. The del.11/del.11 genotype, which showed a double-muscled phenotype was found in BB cattle and BB x PO F2 cattle. The +/del.11 genotype was found in BB x PO F1 cattle and BB x PO F2 cattle. The +/+ genotype, which showed a normal phenotype was only detected in PO cattle. There was a significant difference of the MSTN gene expression in the sampled animals among genotypes and between phenotypes (normal-muscled vs double muscled). The MSTN expression in animals with del.11/del.11 genotype was higher than that in animals with +/del11 and +/+ genotypes (P<0.05). Animals with +/+ genotype showed the lowest MSTN expression. It was concluded that double-muscled animals showed higher MSTN expression than normal-muscled animals
Proportion of Sawdust as Carbon Sources in Rabbit Manure Compost for Increasing the Growth of Pennisetum purpureum cv Mott
Full text linkThe right proportion of carbon in high N organic matter source in composting process will result good decomposition process. In this study, rabbit manure was composted with different portion of sawdust, and then the chemical properties were evaluated. The compost then applied to determine the growth response of dwarf elephant grass (Pennisetum purpureum cv. Mott) in terms of plant height, number of leaves, root and shoot dry matter, shoot and shoot ratio, and N content in shoot. The results showed that compost consisted of rabbit manure + sawdust with the ratio of 3:1 and 1:1 had good chemical properties (pH around 7.56 - 7.94, C/N ratio 17 – 19, C- organic 19 – 24%, Nitrogen 0.84 – 1.31%, Phosphor 0.43 – 0.82%, and Potassium 0.27– 0.37%) as well as sole rabbit manure compost. Applying compost to Mott grass resulted a good growth response which reflected in plant height (78.29 - 83.46 cm/plant), leaves number (53.50 - 57.92 blades), shoot dry matter (39.69 – 54.56 g DM/plant), root dry matter (16.50 – 18.16 g DM/plant), shoot : root ratios (2.52 - 3.20), and shoot N content (37.14 - 48.55 g DM/plant). The study concluded that compost rabbit manure + sawdust with ratio of 3:1 resulted the same growth response with sole rabbit manure compost
Polymorphisms KIF12 GENE|BfaI and its Association on Carcass Characteristics and Lamb Meat Quality
Full text linkOne of gene which involves on mediation an antioxidant cascade in beta cells as an intracellular target of excess fat intake is Kinesin Family 12 (KIF12). This study aims to identify the diversity of SNP g. 9617965 C>T KIF12 and association with carcass characteristic and meat quality. The total sample used were 102 ram DNA samples consisted of 10 barbados cross sheep (BCS), 52 javanese thin tail (JTT), 15 jonggol sheep (JS), 15 garut sheep (GS), and 10 compass agrinak sheep (CAS). Identification of the diversity of KIF12|BfaI was conducted using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. The results showed that the amplification product was 696 bp. The diversity of the KIF12 gene was found three genotypes namely CC, CT, and TT. The allele frequency in all types of sheep was in Hardy-Weinberg equilibrium. A SNP of the KIF12 was associated (P<0.05) with characteristic of carcass, including length of carcass. The association of KIF12 with meat quality was significant (P<0.05) too associated with pH and water holding capasity (WHC). It could be concluded that KIF12 gene is a potential candidate gene for carcass characteristic and meat quality traits in shee
Single Nucleotide Polymorphism of Partial GDF9 Gene in Three Local Goat of Indonesia Compare with Several Goat in Asia
Full text linkThe GDF9 gene is a gene that affects the maturation of oocytes. GDF9 is expressed in oocytes and granulosa cell, it can stimulate granulose cell proliferation and regulate cumulus cell function from pre-ovulation to ovulation. The GDF9 gene is associated with an increase in the ovulation rate and litter size in animals. This study aims to determine the kinship relationship of local goats compared to goats in Asia on prolific traits and to determine the restriction mapping of the GDF9 gene in goats based on the different SNP locations. The local goat comes from the Bligon goat, Kacang goat and Kejobong goats which is compared to the GenBank data (EF446168, EU883989 and KY780296). GDF9 sequences were analyzed using BioEdit and sequencing results to identify Single Nucleotide Polymorphism (SNP) and using NEBCutter V2 to determine the restriction enzyme which recognized the sequence around SNP. The result shows that three variations of SNP were found in exon 2 (g.3615T>C, g.3760T>C and g.3855A>C). Identification of SNP position found 1 SNP position identified by restriction enzyme at g.3855A> C. The identified restriction enzyme is HpaII and MspI. The results of this study are expected to provide genetic information that will be used for further research on the relationship between GDF9 gene polymorphisms to animal prolific
The Quality of Buffalo Sperm Following Preservation Using Different Diluents and Sperm Concentrations
Full text linkArtificial Insemination (AI) success depends on the quality of the frozen semen. The quality of the frozen semen of swamp buffalo in Indonesia is still low. The study was conducted to determine the quality of buffalo sperm following freezing using three different diluents and three different doses. The study used buffalo semen from the Tuah Sakato Artificial Insemination Center, Payakumbuh (n = 3). The semen collecting was carried out once a week for 10 weeks (replication). The research method used was 3x3 factorial randomized block design. The first factor was diluent (Triladyl®, Andromed® and Tris egg- yolk) and the second factor was the dose of spermatozoa (10 and 15 and 20 x 106 sperm/ml). Data were analyzed using variant analysis, while the differences between treatments were tested by Duncan Multiple Range Test. The results showed that the plasma membrane integrity of buffalo sperm was found in Andromed® diluent, while tris egg-yolk diluent gave better motility, viability, plasma membrane integrity and recovery rate at a sperm concentration of 20 x106 sperm/mL compared to triladyl® diluent and a sperm concentration of 10 and 15 x106 sperm/mL. It was concluded that andromed® diluent and tris egg-yolk gave better motility, viability, plasma membrane integrity and recovery rate at a sperm concentration of 20 x106 sperm/mL compared to triladyl® diluent and a sperm concentration of 10 and 15 x106 sperm/mL. of Buffalo of sperm abnormalities not sicnificantly by the type of diluent but are influenced by sperm concentration
The Effect of Thawing Duration on the Post Thawing Quality of Bali Cattle’s Frozen Semen and Conseption Rate in Smallholder Farms of East Lombok Regency
Full text linkThis research aimed to identify the effect of the length of thawing time on the quality of Bali cattle postthaw semen and to investigate the resulting pregnancy rate. This research was conducted at Wanasaba Village, specifically Tanaq Mira Village, Wanasaba District, East Lombok Regency. Five samples of Bali cattle semen were collected for insemination from each of three different farmer groups. The semen sample from the remaining frozen semen inseminated by the inseminator was used in this research. Tanaq Mira Village's inseminator performed thawing during the trip from the artificial insemination (AI) station to the farmer group's location. This research observed the microscopic quality of the postthaw frozen semen covering motility, viability, and abnormality. Moreover, the pregnancy rate on AI acceptors using the non return rate (NRR) parameter or the number of female parents who returned to estrus after being inseminated were observed. The observation was conducted on three farmer groups with different distances and lengths of thawing time. Each farmer group was observed five times. The collected data were then analysed by using one-way analysis of variance(ANOVA). The research indicated that the distance of the farmer group’s location significantly affected (P0,05) the sperm abnormalities. The Sapeng farmer group had the highest NRR, as much as 80%. According to the NRR score, it can be concluded that the thawing process using the thermos within less than 10 minutes resulted in the best yield
Comparison of Productivity of Sentul and Kampung Chickens until the Age of 3 Months in the First Generation Selection Population (G1)
Full text linkThis study aimed to compare the productivity of Sentul chickens and first-generation Kampung chickens (G1) until the age of 3 months. The research material were Sentul chicken and Kampung chicken. The method used was an experiment method or direct observation with a sample of 174 chickens from each strain. The data collected were egg weight, doc weight, body weight, body weight gain, body measurements, and selection response. Data on egg weight, body weight, and body measurements were analyzed using the average difference test (t-test), while the average value vector of body measurements Sentul chicken and Kampung chicken were analyzed using the T2-Hotelling statistical test. To identify the body size and body shape characteristics of Sentul chickens and Kampung chickens were analyzed using principal component analysis. Data processing used the statistical software Minitab version 18. The results of this study showed that egg weight, body weight at the age of DOC-3 months and body measurements of Sentul chickens were significantly different (P<0.05) higher than the Kampung chickens. This study concludes that the egg weight, body weight, body weight gain, and body sizes of Sentul chickens are higher than Kampung chickens. The size characteristic of Sentul and Kampung chickens is chest circumference. The characteristic of the shape of the Sentul chicken is the length of the wings, while the shape of the native chicken is the width of the chest. The selection response and the heritability value of the Sentul chickens was higher than Kampung chikens.
Comparison of Electronic Cow Record (REKS-EL) Feature with Recording Components Used by Dairy Farmers In Sleman Regency, Yogyakarta
Full text linkThe study was conducted in 60 respondents of dairy farmers in Sleman Regency, Yogyakarta from August 2020 to February 2021. The study aims to make comparison between recording in the REKS-EL version 1.0 and those in dairy farms recording, in order to improve REKS-EL features. Data of the farmer characteristics and recording components were collected by interviewed. Identification of components the dairy farmers recording was carried out using a list of questions in a table. The list contains 43 components, which were arranged based on the recording guidelines as written in the Indonesian Minister of Agriculture No. 100, 2014. The result of comparison between dairy farmers recording and REKS-EL version 1.0 feature showed differences on recording content. Software REKS-EL has more recording components than dairy farmers recording, as much as 23 vs. 13 (46% vs. 23% of total components). The REKS-EL feature showed advantages on data of pedigree, and reproduction but needs to be improved with the additional components derived from farmer records, namely daily and weekly milk production. In the REKS-EL feature also needs to be added feed data, body condition score and calf after birth.
The Effect of Using Organik Protein in Feed on Abdominal Fat and Internal Organs of Broiler
Full text linkThe purpose of this research was to determine the effect of organik protein in feed on the quality of abdominal fat and internal organs in broilers. The material used 500 broilers. Levels of organik protein in feed P0 (0% organik protein); P1 (1.5% organik protein); P2 (3.0% organik protein); P3 (4.5% organik protein) and P4 (6.0% organik protein). The variables measured were abdominal fat and internal organs weight of broiler (heart, liver, gizzard, pancreas and spleen). The data were analyzed by using ANOVA and if it shoes significant different among treatment continued by using Duncan’s Multiple Range Test. The result showed that the treatment gave no significant effect (P>0.05) on abdominal fat and weight of heart, liver and spleen. The use of organik protein in feed can reduce `abdominal fat and improve the performance of internal organs such as the gizzard, liver, spleen, pancreas and heart of broilers