Jurnal Agro
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Inisiasi Tunas Kokoleceran (Vatica bantamensis) pada Berbagai Jenis Media Tanam dan Konsentrasi BAP (Benzyl Amino Purine) Secara In Vitro
Get PDFKokoleceran (Vatica bantamensis) is an endemic plant of Banten which is only in Ujung Kulon, and has been designated as identity of Banten province. Now the existence of Kokoleceran has been endangered. Based on data from the IUCN, since 1998 there has been no research. Kokoleceran initiation needs technology for preventing from the extinction. One of the mass propagation is through the technique culture in vitro. This research aimed to get the precise medium and BAP concentration for Kokoleceran, and knowing the response of Kokoleceran growth in in vitro. This research was done in November 2015 until March 2016 at the Laboratory of Biotechnology, Department of Agroecotechnology, Faculty of Agriculture, University of Sultan Ageng Tirtayasa, Serang, Banten. This research used Completely Randomized Design (CRD) two factors. The first factor was medium that were MS and WPM media. The second factor was BAP concentration which consisted of four levels namely 0 mg L-1, 1 mg L-1, 2 mg L-1, and 3 mg L-1. The results showed that the use of different media and BAP concentrations had no impact on the time appear of shoot, shoot number, and root number. There was effect from both treatments on medium color, and growing of callus on the explants
Keragaan Hama, Penyakit, dan Musuh Alami pada Budidaya Padi Organik
Get PDFOrganic rice cultivation intents to minimize the use of inorganic fertilizers and chemical pesticides, but in fact not apart from the disruption of pests and diseases. The study aimed to determine the pests, diseases, and natural enemies on organic rice cultivation. The research was conducted at Screen Field of ICRR at WS 2015/2016 and DS 2016, using Split Plot research design with three replications. The main plot was varieties of: 1). Inpari 25, 2). Inpari 33, and 3). Sintanur. The sub-plot was application of leaf extract of: 1). Mindi, 2). Soursop, 3). Mahogany, and 4). Control. Applications were performed on 4, 6, 8, and 10 weeks after planting (WAP). Observations of pests, diseases, and natural enemies on 5, 7, 9, and 11 WAP as many as 20 clumps/plots. The results showed that varieties and leaf extract applications significantly affected on severity of stem rot disease at 5 and 7 WAP in WS 2015/2016. In the rainy season found Yellowing on Inpari 25 and Inpari 33 varieties. However, Sintanur was more resistant to Yellowing with LCC value on two growing seasons was highest compared to the other varieties. Sintanur had the lowest Cercospora Leaf Spot disease severity compared to the other varieties. The severity of disease generally increased with the age of rice increase, while the pest population density and natural enemies were fluctuate. The density of the pest was quite high in the control plot, while the natural enemy density was high enough in the application plots of Mindi, Mahogany, and Soursop extracts
Aplikasi Berbagai Bahan ZPT Alami untuk Meningkatkan Pertumbuhan Bibit Kemiri Sunan (Reutealis trisperma (Blanco) Airy Shaw)
Get PDFCandlenut cv. Sunan is alternative biofuels plant also as raw of many industrial products. Its hard pulp seed becomes barrier of germination. This condition can be overcome by giving natural plant growth regulators (PGRs), i,e shallot as auxin source, bamboo shoot as gibberellin source, banana bulb and coconut water as sources of cytokinin. The research aim was knowing effect of various PGRs and their combinations to promote germination and growth of seedling candlenut cv Sunan. The research was done from June to October 2016, in experimental design of Randomized Block Design (RBD), consisted of : a0 : control (without PGRs); a1 : shallot (BM); a2 : bamboo shoot (RB); a3: banana bulb (BP); a4 : coconut water (AK); a5 : BM+ RB; a6 : BM + BP; a7 : BM + AK; a8 : RB+ BP; a9 : RB + AK; a10 : BP + AK; a11 : BM + RB + BP + AK, all repeated three times. The result showed that application of natural PGRs had significant effect on germination percentage, plant height on 30 days after planting (dap), 37 dap, 44 dap, and 51 dap,  number of leaves 51 dap. Application of BP + AK gave the best effect on germination (97.78%), but no significant different with RB + BP; BM + RB + BP + AK. Application of BP + AK generated highest plant (13.57 cm). The best of the number of leaves was RB + AK (1.45 leaves). Overall application of natural PGRs from banana bulb + coconut water gave the best effect, besides bamboo shoot and shallot have opportunity to be developed
Pertumbuhan dan Perkembangan Jaringan Meristem Bawang Merah (Allium ascalonicum L.) Kultivar Katumi Secara In Vitro
Get PDFThis study aimed for knowing and obtaining the best concentration of kinetin and NAA interaction effects in influencing the shoot induction, knowing how the plant growth regulators in induction mediastill affect the shoot additionin the MS0media and also knowing the largest number of roots in rooting media for shallot by in vitro. The experiment was conducted at Laboratory of Tissue Culture Seed Technology, Faculty of Agriculture, Padjadjaran University, during January 2011 until May 2011. This experiment divided in 3 stages, namely shoot induction stage, shoot subculture to MS0 media stage and shoot subculture to rooting media stage. Experimental method used in the shoot induction stage was factorial Completely Randomized Design with three replications. The first factor was the kinetin with four levels,0, 1, 2, and 3 mg L-1. The second factor was the NAA with three levels, as 0, 0.01, and 0.1 mg L-1. Basic media used for each treatment was MS. The experiment result showed there was an interaction between kinetin and NAA on shoot induction stagewith the plantlet height, leaf number, and shoot addition. The best result for leaf number was gained from interaction with 2 mg L-1 kinetin without NAA,while the treatment of 2 mg L-1 kinetin with 0.01 mg L-1 NAA gave a better interaction for theshoot addition variable
Daya Hambat Ekstrak Daun Sirsak, Kirinyuh, dan Rimpang Lengkuas terhadap Pertumbuhan Koloni Colletotrichum acutatum
Get PDFSeveral plants that are potentially used as bio-pesticides are soursop, siam weed, and galangal. The research objective was to find out the effectiveness of leaf extract of soursop and C. odorata, and extract of galangal rhizome in inhibiting the growth of Colletotrichum acutatum colonies, causing antracnose on chilli, in in vitro. The experiment was conducted in the laboratory of Agriculture Faculty, Universitas Siliwangi Tasikmalaya from July until August 2016. The research design used was a completely randomized design consisted of nine treatments and three replications. The treatments were A ( control); B (soursop leaf extract, 0,5%); C (soursop leaf extract, 1%); D (C. odorata leaf extract, 0,5 %); E (C. odorata leaf extract 1%); F (galangal rhizome extract 0,5%); G (galangal rhizome extract 1%); H (mixture of soursop leaf extract, C. odorata leaf extract, and galangal rhizome extract each 0,5%; and I (mixture of soursop leaf extract, C. odorata leaf extract, and galangal rhizome extract each 1%). The results showed that the mixture of each 1% soursop leaf extract, C.odorata leaf extract and galangal rhizome extract was effective in inhibiting the growth of C. acutatum colonies at 7 and 14 days incubation period by 66,19% and 69,94% respectively. The three extracts are potentials as anti-fungus of C. acutatum
Manajemen Pemupukan N-P-K dengan Pupuk Hayati untuk Mempertahankan Kandungan N dan P Tanah serta Hasil Kedelai
Get PDFOne effort to increase the productivity of soybeans in Inceptisols Jatinangor is through utilize inoculant of soil microorganisms such as nitrogen-fixing bacteria and phosphate-solubilizing bacteria in the form of specific biofertilizer (SB) for soybean plant without leaving inorganic fertilizer as a source of available nutrients to plants. This research aimed to determine fertilization management of N-P-K and specific biofertilizer in retaining N and P availability and yield of soybean. The Research was conducted from April to July 2016, arranged in Simple Randomized Block Design (RBD) and consisted of 10 treatments. The combination of biofertilizer and N-P-K were as follows; A = control (without fertilizer), B = Standard N-P-K, C = 0 N-P-K + 1 SB , D = 1/4 N-P-K + 1 SB, E = 1/2 N-P-K + 1 SB, F = 3/4 N-P-K + 1 SB, G = 1 N-P-K + 1 SB, H = 3/4 + 1/4 SB, I = 3/4 N-P-K + 1/2 SB dan J = 3/4 N-P-K + 3/4 SB. The results showed a dose of N-P-K and biofertilizer was recommended in the treatment E (250 kg ha-1 SB) and 1/2 N-P-K (37.5-50-50 kg ha-1). The dose was able to increase nitrogen and phosphor content in soil and plant, also on yield of soybean up to 0.4 t ha-1 or 32% of a standard N-P-K
Keragaman Genetik dan Kekerabatan Genotip Kacang Bambara (Vigna subteranea L.) Lokal Jawa Barat
Get PDFBambara groundnut (Vigna subteranea L.) is one of underutliized crops in Indonesia. Bambara groundnut is potential to be developed and can be utilized as an alternative food source in Indonesia. Bambara groundnut greatly varies and has a very wide area of adaptation. The experiment was conducted at the experimental field station at Ciparanje in Padjadjaran University. Starting on September 2014 until March 2015 with Randomized Block Design (RBD) and repeated two times. The research used 30 accessions originally from various locations in West Java (Bandung, Tasikmalaya, Garut, Sumedang, Bogor, Majalengka) and East Java (Lamongan, Madura). Genetic variability of Bambara groundnut landrace  in some West Java showed broad criteria on the characters fresh pod weight, dry pod weight, weight of 100 seeds, and weight per plot. Genotypes which had many similarities in some characters based on euclidian distance coefficient had close relationship
Kompatibilitas Jamur Entomopatogenik Paecilomyces fumosoroseus dengan Beberapa Bahan Aktif Pestisida Secara In Vitro
Get PDFUntuk mengurangi penggunaan pestisida di perkebunan teh, maka salah satu upaya pengendalian hama yang dapat dilakukan adalah mengkombinasikan aplikasi pestisida dengan jamur entomopatogenik. Penelitian ini bertujuan untuk mengetahui kompatibilitas antara jamur P. fumosoroseus  dengan beberapa bahan aktif pestisida di laboratorium. Perlakuan dirancang dengan Rancangan Acak Lengkap (RAL) terdiri dari 5 jenis bahan aktif yaitu metomil, bifentrin, imidakloprid, tembaga oksida dan metidation pada taraf konsentrasi sesuai rekomendasi lapang (RL), 0,5x RL dan 2x RL. Hasil pengamatan menunjukkan bahwa diantara kelima jenis bahan aktif pestisida yang diuji, metidation memiliki tingkat toksisitas yang paling tinggi. Persentase produksi spora tertinggi sebesar 13,77% ditunjukkan oleh perlakuan bifentrin 2x RL. Combining application of pesticides and entomopathogenic fungi is an alternative way for controlling pest in order to reduce pesticides application in the tea field. This research was aimed toexamine the compatibility of Paecilomyces fumosoroseus tosome active agents of pesticide in laboratory. Completely Randomized Block Designed (CRBD) with 5 active agents: methomyl, bifenthrin, imidacloprid, copper oxide and metidation in three different concentration was employed in this study, which was based on the existing field recommendation (FR): 0,5x FR and 2x FR. The results showed that among the five tested pesticides, metidation appeared to be the most toxic agentto P. fumosoroseus. Meanwhile, the highest percentage of spore production was counted in bifenthrin 2x RL (13,77%.
Pengendalian Alternatif Hama Serangga Sayuran dengan Menggunakan Perangkap Kertas
Get PDFSayuran merupakan bahan pangan yang sangat dibutuhkan manusia karena mengandung serat dan sejumlah vitamin dan mineral. Untuk memenuhi kebutuhan masyarakat akan sayuran banyak kendala yang dihadapi petani, salah satunya adalah gangguan hama serangga. Untuk mengendalikan hama serangga telah banyak pula cara yang dilakukan oleh petani. Penelitian ini bertujuan untuk menguji model perangkap serangga yang terbuat dari media kertas dan lampu warna. Warna yang dicobakan dalam penelitian ini terdiri dari merah, kuning, dan hijau. Penelitian ini telah dilakukan dari bulan Juli sampai dengan Agustus 2016 di kebun percobaan Sekolah Menengah Kejuruan Pembangunan Pertanian (SMKPP), Kecamatan Lembah Seulawah, Kabupaten Aceh Besar, Provinsi Aceh. Objek penelitian adalah tanaman jagung. Metode penelitian menggunakan RCBD dengan 3 perlakuan dan 4 ulangan. Variabel pengamatan populasi serangga yang terperangkap pada media kertas dan lampu warna. Hasil penelitian menujukkan tangkapan serangga tertinggi pada kertas dan lampu warna kuning, kemudian diikuti kertas dan lampu warna hijau dan merah. This study aimed to test the two models of trap insect, which were made from trapping paper media and colored light. The tested colors are red, yellow, and green, while the colors of light consist of red, yellow, and green. This study conducted from July to August 2016 at the experimental field of "Hight School Vocational Agricultural Development†Seulawah Valley Districts, Large Districts of Aceh, Aceh Province using the corn crop as the object. The research method approached in Randomized Completely Block Design (RCBD) with 3 treatments and 4 replications. The variable observation was insect populations trapped on paper media and colored light. The result showed that the highest insect population was on paper and yellow color light, the medium population was on the green paper, and the lowest population encountered on red paper
Studi Tingkat Ploidi pada Lili (Lilium sp.) Hasil Kultur Antera Melalui Penghitungan Jumlah Kloroplas dan Kromosom
Get PDFLili (Lilium sp.) termasuk famili Liliaceae, merupakan tanaman hias yang memiliki nilai ekonomi tinggi karena permintaan konsumen terus meningkat. Permintaan varietas tanaman yang seragam menuntut pengembangan hibrida F1. Perakitan tanaman hibrida dapat dihasilkan melalui pembentukan tanaman haploid. Salah satu metode untuk memproduksi tanaman haploid adalah dengan kultur antera. Pengecekan tanaman hasil kultur antera dapat dilakukan dengan penghitungan jumlah kloroplas dan jumlah kromosom, namun untuk lili hasil belum diperoleh informasi mengenai korelasi antara jumlah kloroplas pada sel penjaga stomata dengan jumlah kromosom, sehingga tujuan penelitian ini adalah untuk mengatahui korelasi antara jumlah kloroplas dengan jumlah kromosom serta mengetahui tingkat ploidi pada regeneran lili hasil kultur antera. Penelitian ini dilaksanakan di Laboratorium Kultur Jaringan Balai Penelitian Tanaman Hias (BALITHI) Cianjur dari Januari-Juni 2016. Terdapat 5 nomor lili yang diuji tingkat ploidinya. Setiap nomor terdiri dari 4 ulangan, setiap ulangan ada 4 botol dan masing-masing botol terdiri dari 3 planlet. Metode analisis yang digunakan yaitu statistik sederhana rata-rata dan standar eror dan dikorelasikan. Hasil penelitian menunjukkan planlet haploid terbanyak ditemukan pada nomor 2015.1.1 kelompok Longiflorum dengan jumlah 26,67% sedangkan planlet haploid yang jumlahnya paling sedikit ditemukan pada nomor 2015.S2.3 kelompok Oriental dengan jumlah 11,11%. Metode kultur antera dapat menghasilkan planlet haploid namun pada Lilium sp. persentase keberhasilannya masih rendah. Tidak diperoleh korelasi antara jumlah kloroplas dan kromosom. Penelitian lebih lanjut terkait tingkat ploidi tanaman lili dan pengujian jumlah kromosom dengan menggunakan flowcytometer perlu dilakukan.  Lily (Lilium sp.), Liliaceae family, is an ornamental plant that has a high economic value as consumer demand continues to rise. The uniformity of crop varieties requires the development of F1 hybrids that can be generated through the formation of haploid plants. A method for producing haploid plant is by anther culture. Evaluation of anther culture can be done by counting the number of chloroplasts and the number of chromosomes in the regenerants. However, lilies yet obtained information on the correlation between the numbers of chloroplasts in stomatal guard cells with chromosome numbers. Therefore, the purpose of this research aimed to know the correlation between the number of chloroplasts and chromosomes and to determine ploidy level in the regenerants of lily from anther culture. This research was conducted at Tissue Culture Laboratory of Indonesian Ornamental Crops Research Institute (IOCRI), Cianjur from January to June 2016. There were 5 numbers of lilies regenerant from anther culture that evaluate for ploidy level test. Each number has four replications, each replication contained four bottles, and each bottle has three plantlets. The statistical analysis used statistical descriptive with average, standard error and correlation. The results showed, haploid plantlets were observed in 2015.1.1 plantlets from Longiflorum group with 26,67% while least number of haploid plantlets is found in 2015.S2.3 plantlets from Oriental group (11,11%). The method of anther culture is able to produce haploid plantlets but the success rate was low in Lilium sp. There were no correlation between the number of chloroplasts and chromosomes. Further studies related to the ploidy level of lilies from anther culture and the evaluation of chromosomes number by using flow cytometry requires to develop haploid plant of Lily