Journal of Integrated -OMICS
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Proteomics Analysis of Morphogenic Changes of Human Umbilical Vein Endothelial Cells Induced by a Phorbol-Ester Mimicking Angiogenesis: DOI: 10.5584/jiomics.v3i2.154
No full textPhorbol 12-myristate 13-acetate (PMA) can induce proliferation and migration of endothelial cells, mimicking vessel formation. We analysed by two-dimensional electrophoresis and MALDI-TOF/TOF the effects of PMA on cultured Human Umbilical Vein Endothelial Cells (HUVECs) to further investigate the complex mechanisms related to protein kinase C activation in this angiogenesis model. At 1 μg/ml for 24 hours, PMA induced transition of HUVECs from quiescent type into the proliferative-migrating phenotype. After 2D gel analysis, 15 differences were detected between PMA-treated samples and controls, including 8 increased proteins and 7 decreased proteins. The three main proteins identified by mass spectrometry and increased after PMA are directly involved in cell stress (α-glucosidase, heat-shock protein 70, and 150 kDa oxygen-regulated protein). Four other proteins varied in function of time, two increasing after PMA (heat shock protein 90β, protein-disulfide isomerase A3), and two other decreasing after this treatment (glucose-related protein 75, cathepsin B). These four proteins are involved in protein folding, apoptosis or tumour dissemination. Our data show that phorbol esters modify a number of proteins involved in multiple and intricate pathways for promoting a phenotype ensuring cell survival and cell migration for new vessels formation
Arabidopsis thaliana and omics approaches: a review: DOI: 10.5584/jiomics.v5i1.179
No full textArabidopsis thaliana is a small, flowering plant that is widely used as a model organism in plant biology, mainly because it is the first plant to have its entire genome sequenced. It has since proven to be an ideal organism for studying plant development. Arabidopsis is commonly used as a model plant for genomics, metabolomics and proteomics studies, and more recently it has been utilized in metallomic studies. Because of its widespread applications, many methods for Arabidopsis sample preparation, analytes separation and data quantification have been explored. This review briefly describes the Arabidopsis thaliana characteristics, the developed researches and the primary methods using this plant in different fields of OMICS. In the future, the availability of Arabidopsis genomic information may result in its continuous development for nanoparticles and metallomics studies
Indicative of Violence in Homicidal Women: DOI: 10.5584/jiomics.v8i1.240
No full textThe purpose of the multidisciplinary investigation was to analyze the participation of violence in homicidal women (Ferro, 2012:121-129), from the criminological aspects; By means of the field work done at the Preventive and Female Rehabilitation Center of Puente Grande, Jalisco, Mexico (2008-2014).In methodology, criminological, sociological and law research methods were handled; with interviews with female inmates accused of homicide; including their life history, using a sample of 25%, and analyzing the psychobiological and socio-legal variables.In the discussion and results, there is influence of psychobiological and social aspects in the violent behavior of women, and who has had a life history impregnated with violence, mainly by the family, patron (Lima, 1991) who repeat with children and/or against those who come to affect them, feeling threatened in their integrity. In this complex analysis there are several indicators and external and internal agents that encourage women to apply violence. This entails an interfactorial and multifactorial relationship between related causalities, as in the study of criminal Victimology (Marchiori, 2000).It is concluded that violent acts carried out by homicides come from a combination of multifactorial aspects, in addition to the change of role from victim to victim, in 90% of thecases, which brings with it psychological and social problems in the behavior of The homicidas, being added in some of them the mental illnesses and with a high degree of aggressiveness
Special Issue: Selected abstracts of the VI International Caparica Conference on Analytical Proteomics (ICAP 2019): DOI: 10.5584/jiomics.v9i2.313
No full textSpecial Issue: Selected abstracts of the VI International Caparica Conference on Analytical Proteomics (ICAP 2019
S-glutathionylation of proteins in various types of neurodegenerative pathology and protective effects of pantothenic acid derivatives: DOI: 10.5584/jiomics.v10i1.307
No full textWe studied changes in S-glutathionylated proteins (PSSG) content in rat brain structures in different experimental models of neurodegenerative pathology, as well as the possibility of correcting these changes with pantothenic acid derivatives. We have shown that the content of PSSG significantly increases in brain structures in all the models of neurotoxicosis that we studied, and this increase is observed to the greatest extent precisely in those structures where a particular neurotoxin has the most pronounced effect. Thus, the content of PSSG is a sensitive marker of post-translational protein modification. Precursors of CoA reduce S-glutathionylation of proteins, since HPA, which is not a precursor of CoA, does not have a protective effect in relation to PSSG
Integrated Omics Analysis of Sjogren’s Syndrome: DOI: 10.5584/jiomics.v2i2.97
No full textSjögren's syndrome (SS) is a chronic autoimmune disorder clinically characterized by dry mouth and eyes. The pathogenic mechanism of SS is inadequately understood and a long delay from the start of the symptoms to final diagnosis has been frequently observed. In this paper, we aim to provide an overview about using omics technologies to discover biomarkers for SS diagnosis and understand potential pathways underlying SS pathogenesis. Omics databases relevant to SS such as Sjögren’s Syndrome Knowledge Base, Saliva Ontology and SDxMart are also discussed
