Nature Precedings
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A potential diagnostic biomarker: Proteasome LMP2/b1i-differential expression in human uterus neoplasm
Get PDFUterine leiomyosarcoma (ULMS) develops more often in the muscle tissue layer of the uterine body than in the uterine cervix. The development of gynecologic tumors is often correlated with female hormone secretion; however, the development of uterine ULMS is not substantially correlated with hormonal conditions, and the risk factors are not yet known. Importantly, a diagnostic-biomarker which distinguishes malignant ULMS from benign tumor leiomyoma (LMA) is yet to be established. Accordingly, it is necessary to analyze risk factors associated with uterine ULMS, to establish a treatment method. Proteasome low-molecular mass polypeptide 2(LMP2)/b1i-deficient mice spontaneously develop uterine LMS, with a disease prevalence of ~40% by 14 months of age. We found LMP2/b1i expression to be absent in human LMS, but present in human LMA. Therefore, defective-LMP2/b1i expression may be one of the risk factors for ULMS. LMP2/b1i is a potential diagnostic-biomarker for uterine ULMS, and may be a targeted-molecule for a new therapeutic approach
Computational system biology
Get PDFSystems biology is a multi-disciplinary field that deals with mechanisms involved in complex biological processes by considering them as integrated systems of multiple interacting components. The huge amount of data involved in this study necessitates the use of computational tools
Synchronized Mammalian Cells in Culture: A Comparison of Normal and KB Cancer Cell Growth
Get PDFExperimentation and tests with cells in culture and cell synchronization or synchrony are important in order to be able to make both reliable and high-sensitivity measurements. This is also very important for mechanistic studies of cell division control, oncogenesis and tumor growth. A comparison is made in this article between the initially synchronized cell growth of rabbit and KB cells in synchronized cultures
Visual Molecular Dynamics Investigations of the Impact of Hydrophobic Nanoparticles on Prognosis of Alzheimer’s Disease and Cancers
Get PDFThe possible impact of hydrophobic lectin nanoparticles on the prognosis and progression of Alzheimer's disease (AD) and cancers was investigated by Visual Molecular Dynamics (VMD) computer modeling programs available from the Beckmann Advanced Research Institute at the University of Illinois at Urbana. Our results indicate the possibility of impeding pathological aggregation of certain proteins such as modified tau- or beta-amyloid that are currently being considered as possible causes of Alzheimer's disease. VMD programs serve as useful tools for investigation hydrophobic protein aggregation that may play a role in aging of human populations
A call for disruptive innovation in science publishing with a new open data-sharing platform for the life sciences
Get PDF“A disruptive innovation is an innovation that helps create a new market and value network, and eventually goes on to disrupt an existing market and value network (over a few years or decades), displacing an earlier technology. The term is used in business and technology literature to describe innovations that improve a product or service in ways that the market does not expect.” -Wikipedia
On April 3rd, 2012 Nature Precedings, Nature Publishing Group’s experiment in free pre-print publishing was shut down and no longer accepts submissions. According to the Nature Precedings website it was created in 2007 as “a place for researchers to share documents, including presentations, posters, white papers, technical papers, supplementary findings, and non-peer-reviewed manuscripts.” It was designed to “provide a rapid means for scientists to share preliminary findings, disseminate emerging results, solicit community feedback, and claim priority over discoveries.” It was designed in a way to “make such material easy to archive, share and cite.” Now that Nature Precedings is no more, a new disruptive open data-sharing platform (ODSP) for the life sciences is needed. Based, in part, by the model Nature Precedings established. Here I propose 5 qualities of an ideal ODSP and outline 10 benefits (see Table 1) to scientists for embracing such a potentially disruptive model
An integrated solution for rapid biosensing combining linker free binding, freeze drying and high sensitivity ellipsometric detection
Get PDFA novel integrated biosensor methodology is proposed and demonstrated. The methodology utilizes a nitrogen-containing plasma polymer to achieve linker-free binding of biorecognition molecules that allows the sensor surface to be freeze dried for long shelf life under ambient conditions. The sensor is configured for single wavelength ellipsometric detection providing a low cost, versatile, and rapid sensing and diagnosis platform suitable for a wide range of applications and end-users. The merits of the methodology were demonstrated using three antigen-antibody pairs
The legacy of bio-molecules as a bio-fertilizer: Context of single cell fertilizer Isolation and partial characterization of an amylolytic bacterium
Get PDFRepeated cultivation of crop plants is the reason for the depletion of nutrients in an agricultural land. Therefore, modern procedures of agriculture cascaded with the addition of organic and inorganic fertilizers, the use of insecticides and pesticides, the addition of proper water, etc. Various inorganic molecules are used as fertilizers. However, the use of organic manures is also in practice. They have many roles such as improving soil porosity, air holding capacity, water holding capacity, structure, texture, etc. Agricultural scientists suggest using organic molecules for many reasons. Bio-fertilizers of many kinds are used by farmers of all nations. However, these fertilizers are unable to cause tremendous effects on the growth and development of crop plants, even though these fertilizers have cumulative effects. The present work focuses on the use of bio-molecules as bio-fertilizer. To make these molecules, an amylolytic bacterium was isolated and partially identified based on microscopic observations and biochemical tests. The optimum pH, temperature, substrate concentration, etc. were studied. The optimum pH and temperature for the growth of the isolate were pH 7.0 and 37.0°C, respectively. However, the organism grows even in 60.0°C. The organism uses four commonly available natural substrates as carbon source. Among these, potato starch is the most conveniently utilized by the organism. The amy gene of the strain was cloned using a vector. It expressed a high amount of amylase (data is not shown). The recombinant organism was used to make bio-molecules. It was grown in the presence of various natural substrates and enzymatic activities, and other associated studies were also carried out. The experimental results obtained in this study showed that the recombinant organism can be utilized to make a huge amount of bio-molecules. It will be a unique fertilizer for future generations
Incidence response to a suddenly increased cancer risk
Get PDFIncidence rates of several cancers, including melanoma of the skin and breast cancer, have increased since the middle of the 20th century in Western countries. Here, we developed a model for cancer incidence over time, when considering a suddenly increased cancer risk from an environmental change. A two parameter exponential function was used to simulate cancer risk over calendar time since birth, taking into account the time lapse since the environmental change took place. The calculated incidence for all age cohorts over calendar time was used to calculate the age-standardized rates. The best fit between calculated and reported age-standardized rates was sought by parameter variation. The model was tested using reported rates for melanoma and breast cancer. The best fit was obtained when an increased cancer risk took place from 1955 and with the starting age being that of puberty. Age-standardized rates of melanoma and breast cancer were easily modeled and fit well with reported data. In addition, age-specific rates fit well with reported data without further parameter adjustments. The good fit with reported data when using this simple exponential model strongly supports the hypothesis that an environmental change in the mid-20th century was the main cause of the cancer epidemic noted after 1955. Other forms of cancer should be analyzed in a similar manner
PROM Khad an efficient source of P to replace the costly chemical Phosphatic Fertilizer
Get PDFThe subsidy on phosphate fertilizers rose to an unacceptable level while alternate cost effective technologies are available. Fertilizer industry needs to develop innovative products using the recent advances in the agricultural sciences. Manure producing industries to be supplied rock phosphate mineral (at subsidized costs) as being supplied to chemical fertilizers industries suitable to produce PROM Khad (Phosphate Rich Organic Manure)
Some Computational Aspects of Essential Properties of Evolution and Life
Get PDFWhile evolution has inspired algorithmic methods of heuristic optimisation, little has been done in the way of using concepts of computation to advance our understanding of salient aspects of biological evolution. We argue that under reasonable assumptions, interesting conclusions can be drawn that are of relevance to behavioural evolution. We will focus on two important features of life--robustness and fitness optimisation--which, we will argue, are related to algorithmic probability and to the thermodynamics of computation, subjects that may be capable of explaining and modelling key features of living organisms, and which can be used in understanding and formulating algorithms of evolutionary computation