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    Monitoring the in-situ Bioremediation of Spend engine-oil contaminated soil After Irrigation with Fermented Chicken-droppings

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    Preparation for the bioremediation of petroleum contaminants is explored to provide a low-cost and capable strategy for biodegradation of contaminants and renewal of soil fertility. In this study, fermented chicken droppings were used as novel in-situ bio-stimulants and bio-augmentation materials. The investigation determines the capability of fermented chicken droppings to biodegrade the residual total petroleum hydrocarbon compounds in-used engine oil contaminated soil \u2013 in the case of auto mechanic shops. The soil was collected at 10 cm depth from the ground, air-dried and sieved with 2.5mm mesh. A 4 kg of soil was weighed into 13 perforated buckets to allow aeration and prevent waterlogging. The setup consists of three replicates that were spiked with 150 ml of used engine oil. 500 ml of the fermented chicken droppings were used to irrigate the contaminated soil at ratio 1:8 periodically for every 3 days per irrigation for 21 days. The uncontaminated soil used as the control group was irrigated with normal water. The TPH composition of the contaminated and remediated soil samples was screened using gas Chromatography tandem mass spectroscopy. Results show that the uncontaminated soil (A-group) had C10 - 24.058 \ub1 0.02 ppm, C12 - 37.327 \ub1 0.01 ppm, C14 - 28.515 \ub1 0.02 ppm and C16 - 12.097 \ub1 0.02 ppm, respectively out of about 35 TPH compounds that ought to be detected from C8 to C40. The Concentration of TPH in Contaminated soil before irrigation with Chicken droppings \u2013 positive control (ppm) B-group gave a significant qualitative and quantitative presence of TPHs in contaminated soil at varying concentrations. 36 TPHs were detected out of forty, starting from C10 - 1.836 \ub1 0.01 ppm to C38 - 50.150\ub10.01 ppm. Whereas, the Concentration of residual TPH in Contaminated soil after irrigation with Chicken droppings gave varying levels of residual TPHs ranging from C8 - 1.519 \ub1 0.02 ppm to C38 - 41.487 \ub10.02 ppm. This also gave a resultant/differences in the degradation level of TPHs. Differences in TPH Concentration between before the irrigation and after irrigation of contaminated soil were calculated, C8 had - 0.317 ppm while another TPHs concentration varies accordingly. From the results, one can be deduced that despite other environmental factors that influence the degradation of TPHs, fermented chicken droppings showed great capability in the degradation of TPHs in the contaminated soil

    Effect of Fungal Infection on Defence Proteins of Musca domestica L. and Variation of Virulence with Temperature

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    With the current global drive for alternative control measures that are safer in comparison to synthetic insecticides, it was considered necessary to assess some fungal entomopathogens for efficacy against the house fly, Musca domestica. The effect of fungal infection on activity of two detoxification enzymes (esterase [EST] and glutathione-S-transferase [GST]) was determined in the laboratory. Effect of temperature on progression of mycosis was also investigated between 15 and 35\ub0C to determine the optimum temperature necessary for successful fungal infection. Two Beauveria bassiana strains (ARSEF 1564 and ARSEF 8891), Metarhizium robertsii (ARSEF 1057) and M. anisopliae s.s. (ARSEF 5471) were evaluated in this study as potential sources of mycoinsecticide formulations. The fungal strains infected larval and adult house flies successfully by inhibiting activity of EST and GST with a concomitant insect mortality of > 90%. A significantly higher number of dead specimens (80-100%) had fungal growth between 20 and 25\ub0C with a noticeable level of mycosis also at 30\ub0C. The evaluated fungal strains showed good insecticidal efficacy and could, therefore, serve as good sources of mycoinsecticide formulations against house fly. If this area of biological control is well explored it would reduce reliance on synthetic insecticide formulations

    Chemical Speciation of some Heavy Metals in Sand Filter Media [SFM] Waste Dumpsite in Yenagoa, Nigeria

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    The chemical speciation of five heavy metals [HMs] in spent sand-filter-media [SFM] waste dumpsite was investigated with a view to ascertain their distribution and chemical availability of the heavy metals in the dumpsite. Samples were collected at the surface, 100cm depth of the dumpsite and 100m away from the dumpsite and analysed using FAAS for total concentration of Cr, Fe, Mn. Ni and Pb. Speciation analysis was carried out using modified Tessier\u2019s sequential extraction method. The percentage concentrations showed that Pb occurred more at the exchangeable fraction with 90.6% at the top, 91.34% at 100cm depth. This signifies that Pb is biochemically available for in-take by living organisms. Fe had the highest concentration by mass 12mg/g but its occurrence of between 22-28% is in the residual/inert fraction. Ni had 40% in the exchangeable and carbonate fractions at the surface and at 100cm depth. Cr had highest exchangeable fraction of 46.29% and 45.59% at the surface and 100cm depth of the SFM. Mn had 37.30% and 42.26% at the surface and at 100cm depth of the SFM. Although Fe had highest concentration by mass, it is least biochemically available occurring more at the residual/inert fraction while the other four metals [Pb, Ni, Cr, Mn] are all biochemically available for intake by organisms and may enhance biochemical accumulation overtime and finally become harmful to living organisms around that dumpsite

    Ameliorating Effects of Aqueous Extract of Tetracarpidium conophorhum against Iron- Overload Induced damage in Rats

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    Iron, though an important nutrient but in excess can produce serious damaging effects on organs of the body. Intake of plants and or plant products with iron chelating ability, instead of synthetic iron chelators, can produce less adverse effects and may be more effective. The potential effect of Tetracarpidium conophorhum (T. conophorhum) as a chelating agent of plant origin has not been determined hence the present study. Iron overload condition was induced by oral administration of iron II chloride in the rats. Twenty five rats were randomly divided into five groups. Groups A and B were the normal and negative control (iron overload only) respectively. Groups C-E were the iron overload induced rats treated with 250, 500 and 1000mg/kg body weight of T. conophorhum extracts. Animals in groups C-E received daily oral administration of extracts for thirty days. Thereafter, we studied the effects of T. conophorhum on liver function enzymes, antioxidant enzymes, hematological parameters and lipid peroxidation. Iron overloaded rats showed significant increase (p<0.05) in aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total and direct bilirubin levels and a significant decrease (p<0.05) in total protein, albumin, antioxidant enzymes. T. conophorhum extract at a high dose of 1000mg/kg body weight was able to restore the levels of these parameters to normal. These findings therefore suggest that high dose of T. conophorhum seed extract may be effective in reducing the resultant effects produced in the iron overload-induced liver damage

    Development of a Model and Sensor Based Smart Irrigation System

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    Water availability is a critical variable for virtually every economic activity, including agriculture and industry, the energy sector and public use. Specifically, Farmers could waste thousands of cubic meters of water daily through Irrigation. This project developed a smart irrigation system capable of making autonomous decision of irrigating the soil with an appropriate amount of water required through monitoring of soil irrigation factors. The soil moisture level and the rainfall status were the soil irrigation factors considered. The threshold values of the irrigation factors required for soil to be irrigated were established. Subsequently, a system was developed to sense the soil irrigation factors and monitor when the threshold values are reached so that necessary information is communicated to switch on or off a water pump designed for irrigation purpose. The system is implemented with a PIC16f876A microcontroller. The results of performance evaluation conducted on the system revealed that the system is able to monitor and estimate the soil irrigation factors with good accuracy. Also, the system is able to start the water pump to irrigate soil and stop it as appropriate depending on whether the soil irrigation factors value indicates need for irrigation or not

    A Mathematical Model of a Tuberculosis Transmission Dynamics Incorporating First and Second Line Treatment

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    This paper presents a new mathematical model of a tuberculosis transmission dynamics incorporating first and second line treatment. We calculated a control reproduction number which plays a vital role in biomathematics. The model consists of two equilibrium points namely disease free equilibrium and endemic equilibrium point, it has been shown that the disease free equilibrium point was locally asymptotically stable if the control reproduction number is less than one and also the endemic equilibrium point was locally asymptotically stable if the control reproduction number is greater than one. Numerical simulation was carried out which supported the analytical results

    Water Level and the Potentials for Wetland Formation and Sustainability in Lower Ogun River Floodplain, Nigeria

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    Identification, delineation and monitoring of wetlands which requires the understanding and application of their ecological characteristics are critical factors in wetland management and conservation. This study assessed the potentials of wetland formation and sustainability in the lower Ogun river basin by determining the levels of surface and ground water. The study area was divided into 1 km2 grids of 67 units using 0.052 % sampling intensity for the assessments in dry and wet seasons. Monthly water table levels were assessed in 50 cm soil pit while inundation levels were measured during the flooding event using staff gauge. Assessment was carried out for one year during which frequency and periodicity of water table and inundation events were recorded. Results revealed that low water table level ranged from 5 to 45cm of soil depth and occurred between August and September within 6 grids. Water table and surface water was recorded within a period of 2 \u2013 10 weeks during the rainy season. The peak of water levels coincided with the peak of precipitation (> 200 mm) ranging from 30 to 280 cm between September and October while covering 28.75 km2. Conclusively, surface and groundwater levels were recorded during the rainy season having direct variation with the volume of precipitation for a duration that is long enough to support the formation of wetland hydrophytes and hydric soil

    Synthesis and Characterization of ZnO-MMT Nanocomposite for Antibacterial Activity Studies

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    ZnO oxide Nanoparticle and ZnO oxide with Montmorillonite nanocomposite were prepared by an environmentally friendly, efficient, and inexpensive method that was synthesized using the chemical method. ZnO nanoparticles as an effective antibacterial material were immobilized on the surface of montmorillonite (MMT). The objectives of this paper are to summarize our research activities in (a) developing processes to disperse nanomaterials (undoped and doped zinc oxide powders) in the polymers matrix, (b) using X-ray diffraction (XRD), Fourier Transform Infra-Red Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM) and Thermo Gravimetric Analysis (TGA) techniques to characterize polymer matrix structures, (c) studying structure-property relationship of these types of new materials, and (d) evaluating the antibacterial performance of these materials for different applications. The results showed that the ZnO nanocomposite was uniformly dispersed in the polymer matrix and the particles remained their average size (20 - 150 nm) before incorporation into the polymer matrix

    Use of Sedimentological and Geochemical Parameters to Evaluate the Lithologies and Geochemical (Na/Zn and K/Mn) Ratios of OGE-1 Well, Niger Delta Basin

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    This work focuses on subjecting ninety (90) sidewall core well samples to sedimentological and geochemical parameters to determine the lithologies and the geochemical ratios of the OGE-1 Well in the Niger Delta Basin. The sedimentological analysis with sample description yielded lithologies that are sand, shaly sand, sandy shale and shale. The sand lithologies ranged from fine to coarse grained, well sorted to poor sorted at different depths, while the shale lithologies ranged from light to dark coloured shales which is controlled by the organic matter content in the shale. This finally produced a lithologic frame work of the well. The result of the geochemical analysis led to the creation of the geochemical ratio of Na/Zn (0.739 \u2013 5.610) and K/Mn (21.170 \u2013 69.37) for 90 side wall core samples. Na and K had variations in their graphs showing intermittent alternating abundance downhole. Na and K being components of the weathering of feldspars are indicative of abundance of clay minerals. The variation of the Na/Zn and K/Mn profile indicates the alternate deposition of clay and sand size deposits giving the environment of deposition to be paralic

    Assessment of Physicochemical Characteristics of Lower River Niger at Kpata, Adankolo and Gadumo Stations in Kogi State, Nigeria

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    The present work was conducted to study the physicochemical parameters of lower River Niger at Lokoja and Ajaokuta local government area of Kogi state, with the aims to determine the physical and chemical situation of the River at this point in time, compare the physical and chemical situation of the river with earlier work done on River Niger and other relevant water bodies by relevant authors also to determine its suitability for fish growth and survival at this point in time. Water samples were collected for four month (January to April) at three sampling site (two site from Lokoja and one from Ajaokuta local government area) and analysed for various physicochemical parameter. The mean temperature values ranged from 23.55 - 28.350C at Kpata station, 24.65- 29.750C at Adankolo station and 23.90-28.900C at Gadumo station. The mean pH values ranged from 6.69-8.15 at Kpata station, 6.60-8.30 at Adankolo station and 6.10-8.20 at Gadumo station. The mean electrical conductivity values ranged from 68.50-135.50\u3bcS/cm at Kpata station, 68.00-122.50 \u3bcS/cm at Adankolo station and 68.00-122.50 \u3bcS/cm at Gadumo station. The mean dissolved oxygen (DO) values ranged from 6.10-9.30 mg/l at Kpata station, 5.65-7.78 mg/l at Adankolo station and 6.10-9.30 mg/l at Gadumo station. The mean transparency values ranged from 0.31-0.60m at Kpata station, 0.31-0.60m at Adankolo station and 0.30-0.44m at Gadumo station. The mean biochemical oxygen demand (BOD) values ranged from 2.10-2.95 mg/l at Kpata station, 2.05-3.25 mg/l at Adankolo station and 2.10-4.00 mg/l at Gadumo station. The mean nitrate values ranged from 0.02-0.05 mg/l at Kpata station, 0.02-0.03 mg/l at Adankolo station and 0.02-0.04 mg/l at Gadumo station. The mean phosphate values ranged from 0.48-0.61 mg/l at Kpata station, 0.51-0.65 mg/l at Adankolo station and 0.49-0.64 mg/l at Gadumo station. The study indicated that the mean value of the studied parameters which include pH, temperature, dissolved oxygen, Electrical conductivity, biochemical oxygen demand, nitrate, transparency and phosphate of the River compares slightly favourably with the work of other researchers and within the range that support fish growth and survival. This indicates that the river is in a healthy state and is favourable for fish growth and survival

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