Lampung University

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    Pelaksanaan pembiayaan mikro menggunakan AKAD Ijarah di Bank Syariah Mandiri Cabang Kedaton [ CD-Laporan Akhir ] / Fuji Lestari

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    PERBANKAN 332.1 Les

    The complete genomic sequence of Rhinolophus gammaherpesvirus 1isolated from a greater horseshoe bat

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    AbstractIn a comprehensive research project on bat viruses, we successfully isolated a novel herpesvirus from the spleen of a greaterhorseshoe bat (Rhinolophus ferrumequinum) in Japan using a cell line established from the kidney of the same bat. Thisherpesvirus was a novel gammaherpesvirus (Rhinolophus gammaherpesvirus 1; RGHV-1), which belonged to the genusPercavirus. The whole RGHV-1 genome (147,790 bp) showed that 12 of the 84 genes predicted to contain open readingframes did not show any homology to those of other herpesviruses

    Analysis of fitness differences of hepatitis B virus genotypes D and Fusing a cotransfection assay

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    AbstractHepatitis B virus (HBV) circulates as a collection of genetically related variants that evolve throughout the chronic infection.Those viral variants that have the greatest fitness are fixed. We recently showed different fitness for HBV variants involved intwo epidemiological situations. To understand these fitness differences better, we determined the levels of extracellular HBVDNA, the synthesis of HBV DNA intermediates, and the expression of HBeAg and HBsAg in transfection and cotransfectionassays. Our results show that for the subgenotype (sgt) D1, which has an 8-nucleotide deletion (sgtD1del) and exhibits lowerfitness, the levels of extracellular DNA and intracellular replicative intermediates were much lower than with sgtD1wt orsgtD1mut (G1896A), which had higher fitness. In addition, in the cotransfection assay, sgtD1del inhibited sgtD1mut but notsgtD1wt replication. We also found that sgtF1b, which exhibits higher fitness, produces significantly higher levels of bothextracellular DNA and intracellular replicative intermediates than does the lower-fitness sgtF4. These results demonstratea relationship between fitness and the replicative ability of the HBV genome in the transfection assay. In addition, the dataobtained by cotransfecting cells with sgtD1del and sgtD1mut provide new information about the impact of simultaneousreplication of two viral variants in the same cell system on HBV replication

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