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Kinerja keuangan pada PT Ban Lampung Kantor Pusat dengan rasio beban operasioanl dan pendapatan operasional [ CD-Laporan Akhir ] /Amelia
No full textMANAJEMEN KEUANGAN 658.15 Ame
Koordinasi Antar Instansi Pemerintah Terhadap Pemberantasan Peredaran Narkotika : (Studi Kasus Di Kecamatan Tegineneng Kabupaten Pesawaran) [CD-Skripsi] /Diah Wilantika R.
No full textPengembangan Sistem Informasi Pendaftaran Seminar Akademik Di Jurusan Teknik Elektro Menggunakan Metode Rapid Application Development [CD-Skripsi] /Nyoman Herman A.
No full textAnalysis of the efficacy of an adjuvant?based inactivated pandemicH5N1 influenza virus vaccine
No full textInteraction of the intrinsically disordered C?terminal domainof the sesbania mosaic virus RNA?dependent RNA polymerasewith the viral protein P10 in vitro: modulation of the oligomeric stateand polymerase activity
No full textAbstractThe RNA-dependent RNA polymerase (RdRp) of sesbania mosaic virus (SeMV) was previously shown to interact withthe viral protein P10, which led to enhanced polymerase activity. In the present investigation, the equilibrium dissociationconstant for the interaction between the two proteins was determined to be 0.09 ?M using surface plasmon resonance, andthe disordered C-terminal domain of RdRp was shown to be essential for binding to P10. The association with P10 broughtabout a change in the oligomeric state of RdRp, resulting in reduced aggregation and increased polymerase activity. Interestingly,unlike the wild-type RdRp, C-terminal deletion mutants (C del 43 and C del 72) were found to exist predominantlyas monomers and were as active as the RdRp-P10 complex. Thus, either the deletion of the C-terminal disordered domainor its masking by binding to P10 results in the activation of polymerase activity. Further, deletion of the C-terminal 85 residuesof RdRp resulted in complete loss of activity. Mutation of a conserved tyrosine (RdRp Y480) within motif E, locatedbetween 72 and 85 residues from the C-terminus of RdRp, rendered the protein inactive, demonstrating the importance ofmotif E in RNA synthesis in vitro
Queueing theoretic analysis of labor and delivery Understanding management styles and C-section rates
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Complete genome sequence of a novel negevirus isolated from Culextritaeniorhynchus in China
No full textAbstractA novel negevirus, tentatively named Manglie virus (MaV), was isolated from Culex tritaeniorhynchus from the villageof Manglie, Yunnan, China, in August 2011. It was identified by high-throughput sequencing of cell culture supernatants,and the complete genome was sequenced using an Illumina MiSeq sequencer. The complete MaV genome comprised 9,218nt encoding three hypothetical proteins and had a poly(A) tail. BLASTn analysis showed that the genome had the greatestsimilarity to Ngewotan virus strain Nepal22, with query coverage of 100% and 79% identity. Genomic and phylogeneticanalyses demonstrated that MaV should be considered a novel negevirus
Development of a sensitive and reliable reverse transcription dropletdigital PCR assay for the detection of citrus yellow vein clearing virus
No full textAbstractIn 2009, a new viral disease of citrus caused by citrus yellow vein clearing virus (CYVCV) was first discovered in China.CYVCV is considered to be the most serious pathogen affecting lemon production. In this study, a sensitive and reliablereverse transcription droplet digital polymerase chain reaction (RT-ddPCR) assay was developed to detect and quantifyCYVCV without references. The specificity of the assay was demonstrated by its failure to amplify other relevant citrusviruses. The quantitative linearity, sensitivity and accuracy of RT-ddPCR for detecting CYVCV were compared to thoseof real-time RT-PCR. The results showed that both methods had a high degree of linearity (R2 = 0.9776) and quantitativecorrelation. Furthermore, RT-ddPCR was found to be 100 times more sensitive than real-time RT-PCR, and it can thereforebe used to detect CYVCV in individual arthropods. In summary, the results demonstrated that the RT-ddPCR assay is apromising approach for quantitative detection of CYVCV with high precision and accuracy
First report of a novel alphapartitivirus in the basidiomyceteRhizoctonia oryzae?sativae
No full textAbstractRhizoctonia oryzae-sativae is a soil-borne basidiomycete fungus that causes aggregate sheath spot disease on rice worldwide.Here, we report the complete genome sequence of a partitivirus designated as Rhizoctonia oryzae-sativae partitivirus 1(RosPV1) infecting this fungus. The genome of RosPV1 consists of two double-stranded RNA (dsRNA) segments. The largersegment, designated as dsRNA-1 (1,961 bp), contains a single open reading frame (ORF) that encodes a putative polypeptidewith a conserved RNA-dependent RNA polymerase (RdRp) domain. The smaller segment, dsRNA-2 (1,819 bp), also hasa single ORF, which is predicted to encode the capsid protein (CP). BLAST searches and phylogenetic analyses suggestedthat RosPV1 is a representative member of a new species within the genus Alphapartitivirus. This is the first report of analphapartitivirus infecting the fungus R. oryzae-sativae