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    Air Conditioner Condensate and Mineral Water as Alternative Solvents for Potato Dextrose Agar: Comparative Growth of Trichophyton rubrum

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    Trichophyton rubrum is the predominant cause of dermatophytosis worldwide, particularly in tropical countries such as Indonesia. Potato Dextrose Agar (PDA) is a standard medium for fungal culture, conventionally prepared with distilled water; however, its limited availability and high cost in resource-constrained laboratories necessitate alternative solvents. This study aimed to evaluate the growth of T. rubrum on PDA prepared with distilled water, air conditioner (AC) condensate, and bottled mineral water by comparing colony diameter and morphology. A quasi-experimental study with a post-test-only non-equivalent control group design was conducted. PDA was prepared using the three solvents, inoculated by the single-dot method, and incubated at a fixed temperature of 28°C for 7 days. Colony diameter and macroscopic and microscopic morphology were assessed. Statistical analyses included the Shapiro–Wilk test, One-Way ANOVA, and Independent Sample T-test (p < 0.05). All media supported typical T. rubrum growth. Colony diameters obtained using AC condensate were not significantly different from those obtained using distilled water, whereas mineral water resulted in significantly smaller colonies. These findings indicate that AC condensate can serve as an effective and economical substitute for distilled water in PDA preparation, while the use of mineral water should be limited due to variability in composition and lack of standardization.     

    Unmasking the Pandemic\u27s Shadow: A Systematic Review of The Multifaceted Impact of COVID-19 Stigma in Asia

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    The COVID-19 pandemic has exacerbated stigma toward health workers, patients, and survivors while intensifying social inequalities through rising unemployment, poverty, and social exclusion. This systematic review examined literature from ProQuest, Science Direct, Google Scholar, and PubMed, focusing on the stigma of COVID-19 in Asia. Original research employing quantitative or qualitative methods was included for articles that published in 2020-2021, while review articles were excluded. Screening followed PRISMA guidelines, with data collection and analysis conducted from November 2021 to April 2022, resulting in 24 articles. Data extracted included titles, authors, publication years, participants, methods, findings, strengths, and limitations. Quality was assessed using JBI’s critical appraisal tools, and thematic analysis was performed using Braun and Clarke’s framework. The review identified three key themes: general characteristics of COVID-19 stigma, affected populations, and the multifaceted impacts. Stigma in Asia has led to widespread fear and discrimination against infected individuals, survivors, health workers, and vulnerable groups with significant psychological, spiritual, economic, and health repercussions. We recommend that the government raise public awareness to minimize misinformation by providing education about COVID-19 and efforts to address the impacts caused by stigma, as well as integrating anti-stigma measures into policies and expanding mental health services for those affected

    The Effect of Blood Pressure Measurement Training on Early Detection and Control Efforts of Hypertension Patients in Health Service Facilities

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    Hypertension or high blood pressure is the result of systolic and diastolic blood pressure that is too high ≥140/90 mmHg. Hypertension is one of the causes of premature death worldwide Reducing the prevalence of hypertension by 33% in 2020-2030 is one of the global targets of non-communicable diseases. Blood pressure (BP) measurements performed by individuals and families have a high potential to improve the diagnosis and management of hypertension. The implementation of blood pressure measurements is carried out through increasing education and training for individuals and families in measuring blood pressure independently. Early detection through self-measurement of blood pressure and control of hypertension by health services is an effort to control hypertension. This study aimed was to identify the effect of blood pressure measurement training on early detection and control efforts for hypertensive patients at health service facilities. This research uses a pre-experimental research design with a one-group pretest-posttest design. The sampling technique used a purposive sampling method. with a total sample 24 respondents. The form of treatment is providing training to measure blood pressure using a digital blood pressure monitor. Before the training was conducted, the respondents\u27 ability  to measure BP was measured first through a pre-test (initial observation before the intervention, after which the intervention was given (BP measurement), then a post-test (final observation) was conducted after the treatment was given, using the same measuring instrument. The result of this study, there was an effect of blood pressure measurement training on early detection and control efforts of hypertension patients in health care facilities with a p-value of 0.000. Independent blood pressure measurement training in individuals with hypertension has a positive impact on increasing knowledge and changing individual behavior in conducting early detection and control efforts of hypertension in healthcare facilities

    Do Virtual Race Events Improve Physical Activity Behavior?

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    Physical inactivity remains a major global health concern, with approximately one in four adults not meeting recommended activity levels. Virtual competitions have emerged as an alternative approach to encourage physical activity, especially following the COVID-19 pandemic. This study aimed to evaluate the impact of a virtual race event on physical activity behavior in adults with a sedentary lifestyle. A cohort study was conducted involving 68 participants, of whom 32 completed both pre- and post-event assessments. Physical activity levels were measured using the Global Physical Activity Questionnaire (GPAQ) before and four weeks after the virtual race. Participants were categorized into a sedentary group (<600 MET-minutes/week) and an active group (≥600 MET-minutes/week) based on baseline data. The McNemar test was used to assess changes in physical activity classification. Results showed that in the sedentary group, the number of active participants increased from 0 to 5 (41.67%), while in the active group, the number of participants who remained active decreased from 20 to 13 (65%). However, the difference between the groups was not statistically significant (p = 0.56). These findings suggest that while virtual race events may offer motivational value, their short-term effect on physical activity behavior may be limited without additional support mechanisms. Future studies should consider larger samples, longer follow-up periods, and the integration of behavioral strategies to enhance engagement and promote sustained physical activity, especially among previously inactive populations.                 

    The Effect of Self-Resignation Exercise on the Quality of Life of HIV Patients

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    People living with HIV/AIDS (PLWHA) are vulnerable to anxiety related to their illness, which can adversely affect inflammatory markers such as the Neutrophil-to-Lymphocyte Ratio (NLR) and overall quality of life (QOL). This study aimed to examine the effect of Self-Resignation Exercise as a complementary and holistic intervention on NLR and quality of life among HIV patients experiencing anxiety disorders. A randomized controlled trial with a pretest–posttest control group design was conducted in the Infection Subsection of Dr. Moewardi Hospital, Surakarta, starting in January 2024. Participants were HIV patients with anxiety disorders who had stable physical conditions and received routine treatment, and they were randomly assigned to a control group (n = 20) and an intervention group (n = 20). Anxiety levels were assessed using the Hamilton Rating Scale for Anxiety (HRSA), quality of life was measured with the WHOQOL-BREF, and inflammatory status was evaluated through blood tests to determine NLR. The intervention group received Self-Resignation Exercise in addition to standard care, while the control group received standard care alone. The results demonstrated a significant reduction in anxiety levels and a significant improvement in quality of life in the intervention group compared to the control group, with all measured outcomes showing p-values < 0.001. In conclusion, Self-Resignation Exercise is effective in reducing anxiety and improving quality of life in people living with HIV/AIDS, indicating its potential as a complementary approach to enhance both psychological well-being and overall health status

    Phytochemical Compounds of Ethanol Extract Kigelia africana (Lam.) Benth. from Pericarpium, Fructus, and Semen using LCMS/MS and in Silico Study (Potential of Phosphodiesterase-5 Inhibitor)

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    Erectile dysfunction can be treated by pharmacological therapy with phosphodiesterase-5 inhibitors (PDE5-I). One plant that has a function as a phosphodiesterase-5 inhibitor is Kigelia africana (Lam.) Benth. Research on Kigelia africana (Lam.) Benth. An extract as a Phosphodiesterase type 5 (PDE 5) inhibitor is still lacking. This research aims to determine the phytochemical compounds in the 70% ethanol extract of the pericarpium, fructus and semen of Kigelia africana (Lam.) Benth. with LC-MS/MS and to determine the ligand-protein interaction through in-silico studies. In previous studies, no one compared each part of the Kigelia africana (Lam.) Benth. as an aphrodisiac. The results of the identification of the 70% ethanol extract of Kigelia africana (Lam.) Benth. found several alkaloids, flavonoids, iridoids, phenolics, polyphenols, coumarins, steroids and fatty acids. Based on the results of molecular docking on 70% ethanol extract of pericarpium, fructus and semen of Kigelia africana (Lam.) Benth., 18 compounds, 20 compounds and 18 compounds were obtained sequentially, which were analyzed using LC-MS/MS. The sitosterol compound with (∆G = -8.90 kcal/mol) was identified in the three parts of the 70% ethanol extract sample of kunto bimo fruit, which showed the highest affinity for binding to the target protein compared to sildenafil with (∆G = -8.68 kcal/mol). Sitosterol compound has the same amino acid residues as the native ligand, namely ILE A:824, TYR A:664, ALA A:779, ILE A:768, ALA A:767, LEU A:765, ASN A:661, HIS A:613, PHE A:786, VAL A:782, LEU A:804, MET A:816, PHE A:820 in hydrophobic bonds. Phytochemical compounds from Kigelia africana (Lam.) Benth. It has the potential as an alternative additional therapy and a promising source for the discovery of new drugs as aphrodisiacs targeting PDE5

    Obesity Among Female Lecturers During the Covid-19 Pandemic in Aceh, Indonesia: A Retrospective Study

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    The Covid-19 pandemic has led to changes in uncontrolled consumption patterns and decreased physical activity among female teaching staff members. Therefore, they tend to gain weight, leading to non-communicable diseases (NCDs) and comorbidities. In support of the government’s efforts, the prevalence of obesity must be reduced through healthy behavioral changes. This study aimed to identify the cause of obesity among female lecturers in Aceh during the Covid-19 pandemic. This study used a case-control design and was conducted in Aceh Province from March to June 2021. The sample consisted of 65 female lecturers (obese) and 65 controls (non-obese), obtained by purposive sampling. Physical activity, NCDs history, anthropometric, BMI, and blood pressure data were collected and analyzed using the chi-square test at a 95% confidence interval (CI). Results found that Female lecturers in Aceh had a higher prevalence of obesity (55.4%). This was because of age > 45 years (p= 0.013; AOR= 2.42), junk food consumption (p= 0.017; AOR= 2.33), not on a diet (p= 0.034; AOR= 2.13), and less physical activity (p= 0.012; AOR= 2.79). During the Covid-19 pandemic, neither income nor pickle consumption were risk factors for obesity (p > 0.05). However, arthritis (p= 0.034; AOR= 2.12) and hypertension (p= 0.003; AOR= 2.93) were present in a high proportion of obese female lecturers (p < 0.05). Additionally, the risk of obesity during the Covid-19 pandemic in Aceh was unrelated (p > 0.05) to diabetes, high cholesterol, and heart disease. In conclusion, obesity among female lecturers in Aceh was caused by risk factors such as age, junk food consumption, and lack of physical activity during the Covid-19 pandemic. It is also associated with NCDs including arthritis and hypertension. It is hoped that avoiding obesity will prevent the emergence of non-communicable diseases

    Efektivitas ASI Eksklusif Terhadap Tumbuh Kembang Bayi melalui Tes SDIDTK Berbasis Android

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    The future of the country depends on the optimal growth and development of children. About 10% of Indonesia\u27s population are toddlers, and most of them experience growth and development problems. It is important to stimulate, detect and intervene in child development, including exclusive breastfeeding for 6 months. The aim of this study is to examine the effectiveness of exclusive breastfeeding on infant growth and development using the Android-based Stimulation, Early Detection, and Intervention of Child Growth and Development test. The research method utilized is an analytical cross-sectional observational design with 83 respondents purposively selected and conducted from June to August 2023. The data analysis utilized includes the chi-square test, Phi (Φ), and Cramer\u27s V. The instruments included a checklist form, the mobile application for Stimulation, Early Detection, and Intervention of Child Growth and Development version 1.1, a weighing scale, a height measurement tool, and the Maternal and Child Health Book. The research findings indicate that there is no significant relationship between Exclusive Breastfeeding and Infant Growth (p-value = 0.737, phi/Cramer\u27s V = 0.165). However, there is a significant relationship between Exclusive Breastfeeding and Infant Development (p-value = 0.003, phi/Cramer\u27s V = 0.356). It can be concluded that there is no correlation between Exclusive Breastfeeding and infant growth, but there is a relationship with infant development. Therefore, it is recommended to enhance public education regarding the importance of Exclusive Breastfeeding, and further research to understand the relationship between exclusive breastfeeding and infant development, and to apply the findings for targeted intervention programs, particularly utilizing Android-based apps for easier access to information and support for mothers.      

    Healthcare Support Related to Family Awareness Efforts Towards The Risk of Stunting in Children

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    Preventing stunting necessitates collaborative efforts from multiple stakeholders, with the family being the most pertinent social unit in guaranteeing adequate nutrition for children.  The study aimed to examine the correlation between healthcare supports and family efforts in raising awareness about the risk of stunting in young children. This study used a cross-sectional survey that examined family characteristics and various efforts to prevent stunting in children within the community. In addition, the study investigated healthcare support and family awareness. A total of 200 mothers from families with stunted children participated in the study. Data were collected using questionnaires and analyzed descriptively. Rank Spearman Correlation was used to investigate the correlation between healthcare worker support and the behaviors of mothers of toddlers with stunting before marriage, during pregnancy, and while breastfeeding. The average age of mothers was 29.6 years, with the youngest being 20 and the oldest 46 years. The majority of the mothers had a high school education, were housewives, came from nuclear families, had access to clean drinking water, lived in clean and well-maintained homes, and had incomes below the regional minimum wage. Efforts to prevent stunting were fair before pregnancy, during pregnancy, and while breastfeeding. Most of the support from healthcare workers in preventing stunting were classified as good. The analysis results indicate a significant positive relationship between healthcare worker support and the behaviours of mothers of toddlers with stunting, both before marriage and while breastfeeding (p<0.05). Our findings revealed that stunting was observed in families of productive age, with high school education, nuclear family structures, and low economic income below the regional minimum wage.  Preventive efforts have been made since before marriage, during pregnancy, and while breastfeeding, but have not been fully optimized. Support from healthcare workers must be continually improved to meet the healthcare needs of families in an effort to prevent stunting in children

    Probiotic Edamame Yoghurt: A Study on Sensory, Microbiological, and Physicochemical Properties of Fermentation Using Mixed Cultures

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    One of the primary risk factors for cardiovascular diseases (CVDs) that caused deaths is hypercholesterolemia, which can be identified by high levels of total cholesterol, low-density lipoprotein (LDL), and very low-density lipoprotein (VLDL). Dietary interventions, involving the consumption of soy protein, have been indicated to be effective in reducing LDL cholesterol levels. Edamame, a kind of soybean, is a potential source of soy protein that could be processed into functional foods. The aim of the research was to evaluate the chemical and sensory properties of edamame yoghurt fermented with lactic acid bacteria and probiotic bacteria, potentially contributing to a cholesterol-lowering benefit. The research methodology was a two-factor Randomized Block Design (RBD). The edamame yoghurt was made by combining edamame juice, skim milk powder, and sugar, followed by heat (80-85 °C for 15 minutes), and cooling to 45 °C. The mixture was then inoculated with a combination of lactic acid bacteria (Lactobacillus bulgaricus and Streptococcus thermophilus) and probiotic bacteria (Bifidobacterium bifidum and Lactobacillus acidophilus). Fermentation proceeded at 37 °C for 7 hours.  The pH, total titratable acidity, lactic acid bacteria count, and soluble protein content were analysed. Sensory properties were evaluated using preference and hedonic tests. The results exhibited that the combination of bacterial cultures significantly affected (p<0.05) pH, total soluble protein, and sensory properties of the yoghurt. Bifidobacterium bifidum led to higher acidity pH (3.73-3.78), while Lactobacillus acidophilus resulted in the lowest titratable acidity (0.55-0.63%). The combination of Lactobacillus bulgaricus and Streptococcus thermophilus improved flavor, texture, and fermentation efficiency. The yoghurt’s texture was smooth due to proteolytic activity by lactic acid bacteria. Edamame yoghurt\u27s green color fades during fermentation, and its aroma is less favoured according to panelists, but its creamy texture and mildly acidic taste make it appealing overall.  Edamame yoghurt, a source of soy protein fermented with lactic acid and probiotic bacteria, shows potential for cholesterol reduction

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