Toyohashi University of Technology Repository

豊橋技術科学大学博士(工学)doctoral thesi

MEGA, MUTIARA SARI

Mercury Contamination Levels in Small-scale Gold Mining Areas in Indonesia

Nur Liyana Binti , Tajul Lile

Successive Forging of Quenchable Steel,Aluminium and Stainless Steel Sheets Having Thickness Distribution and Inclined Cross Section

978-981-4749-25-1(hardcover)978-981-4749-27-5 (ebook)boo

稲田, 亮史

Inada, Ryoji

Chapter 1.5: AC Loss of Bi-2223 Wires

Toyohashi  University  of  Technology
2 0 1 6
THE  LARK  HILL
Bulletin
of  the  Institute of Liberal Arts
and  Sciences
No.38
Contents
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Mineo Ikematsu
Yasuyuki Nakamori
Manabu Okada
Yuri Shakouchi
Hiroyuki Shibusawa
Yuzuru Miyata
Makoto Yamaguchi
Yosuke Sasao
Makoto Kobayashi
Mihoko Kato
Mineo Ikematsu
Kazuhisa Kawai
David Levin
Eugene Ryan
Yuri Shakouchi
Bovine Odorant Binding Protein
- Binding Model, Its Proof and Practical Applications -
Development of an English Vocabulary Learning Support System for
Science and Technology Students
A Study on the Spatial Computable General Equilibirum Model with
External Economies of Technological Propagation
The Ambiguous Non-Conformism: Representations of the Beatniks in
William F. Brown’ s ?????????????? and Consumerism
W.M.Vories Bibliography


English

CONTENTS（雲雀野 第38号）

イケマツ, ミネオ

池松, 峰男

Ikematsu, Mineo

? ??
Bovine Odorant Binding Protein
– Binding Model, Its Proof and Practical Applications –
Mineo Ikematsu
Abstract
?Bovine odorant-binding protein ?OBPb??among OBP family, assumes a dimeric 
structure. We indirectly confirmed, by measuring the fluorescence of intrinsic 
tryptophan and tyrosine residues of OBPb that odorant enters the central pocket 
formed by the dimerization when OBPb first encounters odorant, and odorant with 
high affinity with OBPb subsequently enters the internal cavity ?suggested binding 
site??releasing the pre-bound odorant. The internal cavity-bound odorant can be 
released by the binding of other odorants at another internal cavity or at the central 
pocket, depending on the affinity of the binding odorants to OBPb.  This ?switching? 
mechanism enables OBPb to bind coming odorants even with pre-bound odorant, and 
hence is considered to be more reactive than monomeric OBP.  However the 
suggested mechanism must be directly confirmed before OBPb?s practical applications 
by some visualization techniques.  Here, we suggested to prove the mechanism using 
a high-speed atomic force microscope that has recently been developed and presented 
some issues to be tackled for OBPb to be practically applied to sensor fields.
? ??
?????????????????????????
?????
???????
??????
??????????????????????????????????????????
???????????????????????????????????????????
???????????????????????????????????????????
???????????????????????????????????????????
???????????????????????????????????????????
????????????????????????????????????????? 1, 2?
???????????????????????????????????????????
?????????????????????? 3, 4????????????????????
? 5, 6???????????????????????????????????????????
???????????????????????????????????????????
???????????????????????????????????????????
???????????????????????Odorant-Binding Protein: OBP?????
OBP ????????????????????????????????????????
??????????????????? OBP?OBPb???????????????????
???????????????????????????????????????????
???OBPb?????????????????????????????????????
???????????????????????????????????????????
????????????????????????
????? ???
OBP???? 7, 8???? 9, 10?????????????????????????OBP??
? ??
??????????????????????????????
???????????????????? 11, 12?????????????????????
???????????????? 13??????????????????????????
???? OBP??????????? 14?????? OBP?OBPb?????????? 15??? 1?
OBPb???????????????????????????? OBP????????
???????????????????? OBPb?????????????OBPb????
???????????????????????????????????????????
??? 15, 16?????????????? OBPb?????????????????????
????????????????????
? ????????????????????
???????????????????????????????????????????????????????
?1?OBPb?????
?????????????? 17??????OBPb????????????????? 2??
????A????????????????????????Central Pocket: CP??????
??????????????????????????? 2???????????B???
?????????????????Internal Cavity: IC??????????????CP??
???????????????? 2???? 1??????C?????? IC???? CP?
????????????? IC?????????????? 2???? 2-1?2-2?????
???OBPb?????????????????????????????????????
??????C????????????? IC?CP???????????????? OBPb
???????????????????????????????????????????
??????????????? 2???????????????????????????
???? 3??????????????Pevsner? 11????????????????????
???????????????????????????????????????????
? ??
???????????????????????????????????????????
???????????????????????????? 4???????
A. ??? C. ??B. IC??
???? 1 ???? 2-1????? ???? 2-2
? ?????? ?????????????
??? ???
????
? ????????????????????????????????? ?? ??????
?? ? ???????????????????? ?????? ?????????????
? ??
??????????????????????????????
?!
? ???????????
?????????????????????????????
?2?OBPb?????????????????
??????????????????????????????????????????
???????????????????????????????????????????
?????????????????????????????????????? IC? CP
???????????????????????????????????????????
? 1?????????????????????????????????????????
???????????????????????????????????????????
????????????????in situ????????????????????? 20?
?????????????????????????????????????High-Speed 
Atomic Force Microscope: HS-AFM?18-20?????????????????? OBPb????
?????????????????OBPb???????????????????????
?????????? 2??????
?????? CP???????
???????? IP? CP?????? IP?????? IP????????
????
?????? ??????????
?1???
??????????????????????????????????????????
???????????????????????????????????????????
???????????????????????????????????????????
?????? HS-AFM?????????????????????? 19??????????
???????????? 21??????????????
? ??
OBPb? 20kDa??????????????????????????????????
???????????OBPb?????????????? HS-AFM???????????
?2?????????
HS-AFM??????????????????????????????????????
???????????????? OBPb???????????????????? OBPb
????????????? CP????????????????????????????
????????????????????????????
? 5????????????OBPb?????????????????????????
??????????????????????????????????????????
CP?????????????????????????????????????????
?????????C?????????????????????????????????
???????????????????????????????????????????
?? C???????????????? 5?a??????????????????????
????CP?????????????????????????????? 5?b?????
???????????????????????????????????????????
???????????????????????????????????????????
???????????????????????????????????????????
??
(b)(a)
IC
? ?????? ????????
????????????????????? ??????????????????????
? ??
??????????????????????????????
?????? ?????????????
?1???????????
OBPb???????????????????????????????????????
???????????????????????????????????????????
????????????????????????????????
????????
??????????????????????????????????????????
???????????????????????????????????????????
???????????????????????????????????????????
???????????????????????????????
??????????????????????????????????????????
???????????????????????????????????????????
OBPb????OBPb?????????????????????????????????
???????????????????????????? OBPb?????????
??????????????? OBPb???????????????????????
????????????????????? OBPb??????????????????
??????????????????????? OBPb????????????????
???????????????? OBPb????? 17????? HS-AFM?????????
??????????????????????????????????????????
OBPb?????????????????? OBPb???????????????????
????????????????
??????
OBPb????????????? IC? CP????? IC? IC?????????????
???????????????????????????????????????????
???????????????????????????????????????????
???????????????????????????? OBPb???????????
?????????????????
??????????????????????????????OBPb?????????
???????????????????????????????????????????
???????????????????????????????OBPb?????????
???????????????????????????????????????????
?????????? 3???????????????????????????????
???????????????????????????????????????????
????????????????????????????????Fluorescence Resonance 
? ??
Energy Transfer: FRET???? 22???????OBPb??????????????????
????????????????????
??????????????????????????????????????????
?????
i =?S/d?dV/dt???? :????S:????d:???V:?????
??? OBPb????????????????????????????????????
???????????????????????????????????????????
???????????????????????????????????????????
???????????????????????
?2????
??????????????????????????????????????????
???????????????????????????????????????????
???????????????????????????????????????????
????????????????????????????OBPb????????? 2??
?????????????????????????????????
??????
OBPb???????????????????????????????????????
????????????????????????????????????? OBPb???
????????????????????????????? 2?????????????
?????????????????OBPb???????????????????????
???????????????????????????????????????????
?????????????????????????????? OBPb?????????
?????????????????????????????????OBPb???????
????????????????????????????????????????????
???????????????????????????????OBPb?????????
???????????
????
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Bovine Odorant Binding Protein– Binding Model, Its Proof and Practical Applications –

長尾, 信義

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MOHD ZAMZURI , BIN MOHAMMAD ZAIN

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渡邊, 悠太

Evaluation of metallic coating by solid particle deposition process and mechanism of particle deposition

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佐藤, 智治

Color Information Representation in the Visual System and Its Task Dependency

Toyohashi University of Technology

Not a member yet

Mercury Contamination Levels in Small-scale Gold Mining Areas in Indonesia

Successive Forging of Quenchable Steel,Aluminium and Stainless Steel Sheets Having Thickness Distribution and Inclined Cross Section

Chapter 1.5: AC Loss of Bi-2223 Wires

CONTENTS（雲雀野第38号）

Bovine Odorant Binding Protein– Binding Model, Its Proof and Practical Applications –

Genome-wide analysis of the extracellular nucleic acids excretion mechanism in Rhodovulum sulfidophilum and its application to RNA production

Studies on the device structure of electrochemically prepared copper oxide photovoltaic devices

Evaluation of metallic coating by solid particle deposition process and mechanism of particle deposition

A Study on an amplified redox sensor integrated with semiconductor technology and electrochemical sensor technology

Color Information Representation in the Visual System and Its Task Dependency

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